FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_B_16_GGCTAC_L005_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_B_16_GGCTAC_L005_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 27772345
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 7804365 3.2806737 3.9328227 16
TGTGT 4358285 2.93924 25.83993 4
TGTTT 4141545 2.2051334 20.49887 6
CTTTG 2869630 1.993499 25.913061 1
GTTTG 2921785 1.9704605 25.052814 7
GAGGG 1748820 1.9412494 7.5683837 11
TTTGA 3417965 1.86708 20.56921 8
TTTGT 3456895 1.8405967 20.206415 2
GTGTT 2605665 1.7572681 24.98326 5
TTGTG 2462620 1.660798 24.985441 3
TGAGG 1885120 1.6520692 9.596191 10
TTGAG 2194665 1.5184851 15.573327 9