##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_B_16_GGCTAC_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	27772345	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.052357408061866	34.0	33.0	34.0	31.0	34.0
2	33.231300957841334	34.0	34.0	34.0	31.0	34.0
3	33.27002224695106	34.0	34.0	34.0	31.0	34.0
4	36.5813221029769	37.0	37.0	37.0	35.0	37.0
5	36.52303080636511	37.0	37.0	37.0	35.0	37.0
6	36.51180827546251	37.0	37.0	37.0	35.0	37.0
7	36.50568459379286	37.0	37.0	37.0	35.0	37.0
8	36.49572637816504	37.0	37.0	37.0	35.0	37.0
9	38.31328164762464	39.0	39.0	39.0	37.0	39.0
10	38.28180234690301	39.0	39.0	39.0	37.0	39.0
11	38.26804754873958	39.0	39.0	39.0	37.0	39.0
12	38.25296970781545	39.0	39.0	39.0	37.0	39.0
13	38.26216443011924	39.0	39.0	39.0	37.0	39.0
14	39.855075111590324	41.0	40.0	41.0	38.0	41.0
15	39.83152067281319	41.0	40.0	41.0	38.0	41.0
16	39.79622775822495	41.0	40.0	41.0	38.0	41.0
17	39.752885217290796	41.0	40.0	41.0	38.0	41.0
18	39.74182421397977	41.0	40.0	41.0	38.0	41.0
19	39.75619149193199	41.0	40.0	41.0	38.0	41.0
20	39.73017683598558	41.0	40.0	41.0	38.0	41.0
21	39.685385407678034	41.0	40.0	41.0	37.0	41.0
22	39.65985609065421	41.0	40.0	41.0	37.0	41.0
23	39.62024222297397	41.0	40.0	41.0	37.0	41.0
24	39.60268558524676	41.0	40.0	41.0	37.0	41.0
25	39.55665090578415	41.0	40.0	41.0	37.0	41.0
26	39.479224062642174	41.0	39.0	41.0	37.0	41.0
27	39.42731814688317	41.0	39.0	41.0	37.0	41.0
28	39.38022882115284	41.0	39.0	41.0	36.0	41.0
29	39.31834769444208	41.0	39.0	41.0	36.0	41.0
30	39.25554201490728	40.0	39.0	41.0	36.0	41.0
31	39.189254382372106	40.0	39.0	41.0	36.0	41.0
32	39.117836754512446	40.0	39.0	41.0	36.0	41.0
33	39.0229809546151	40.0	39.0	41.0	36.0	41.0
34	39.14171514864877	40.0	39.0	41.0	36.0	41.0
35	39.16530602655267	40.0	39.0	41.0	36.0	41.0
36	39.11548790712487	40.0	39.0	41.0	36.0	41.0
37	39.09317221862252	40.0	39.0	41.0	35.0	41.0
38	39.06392110568985	40.0	39.0	41.0	35.0	41.0
39	39.0329076280739	40.0	39.0	41.0	35.0	41.0
40	38.82490041802376	40.0	39.0	41.0	35.0	41.0
41	38.84529235827943	40.0	38.0	41.0	35.0	41.0
42	38.807804166338855	40.0	38.0	41.0	35.0	41.0
43	38.561710363312855	40.0	38.0	41.0	35.0	41.0
44	38.62849075222132	40.0	38.0	41.0	35.0	41.0
45	38.63298263074292	40.0	38.0	41.0	35.0	41.0
46	38.59958714325348	40.0	38.0	41.0	35.0	41.0
47	38.55096960663567	40.0	38.0	41.0	35.0	41.0
48	38.48687599120636	40.0	38.0	41.0	35.0	41.0
49	38.432633506461194	40.0	38.0	41.0	34.0	41.0
50	38.486165608269665	40.0	38.0	41.0	35.0	41.0
51	38.20055623678879	40.0	38.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	9.0
12	23.0
13	47.0
14	81.0
15	149.0
16	309.0
17	524.0
18	929.0
19	1806.0
20	3297.0
21	5754.0
22	10147.0
23	16093.0
24	24332.0
25	34694.0
26	48159.0
27	64434.0
28	85683.0
29	112165.0
30	147214.0
31	190449.0
32	250087.0
33	338281.0
34	496545.0
35	771739.0
36	1216512.0
37	2082755.0
38	4844688.0
39	1.7009429E7
40	16010.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.445594858701234	28.375392870770995	28.172644214342384	24.006368056185394
2	21.88039105935042	28.031828888219557	30.52471252334389	19.563067529086133
3	20.31056274125713	26.866174310076623	31.70748833323731	21.115774615428933
4	21.110553886383098	26.73897555132375	31.14401054606538	21.006460016227766
5	24.532166801182974	26.96543630003156	28.383624069195452	20.11877282959001
6	22.215025211780905	26.759738666544912	30.1132666775504	20.91196944412378
7	24.395815333562936	27.478234193043477	27.65244706559709	20.473503407796496
8	21.591903744534356	27.071556254972347	30.3472465144733	20.989293486019996
9	21.28265006069887	27.01168734581109	30.758306509587147	20.94735608390289
10	21.630031601580637	27.15565070216433	30.30860375672274	20.905713939532294
11	24.13206287397627	27.038514647517527	27.842358273528276	20.98706420497793
12	21.568362340306518	29.660696639048666	27.687103843769766	21.083837176875054
13	23.058600429183482	27.409584185607294	28.244647331865657	21.287168053343567
14	22.65072322844902	27.517129000089835	28.40581520933864	21.4263325621225
15	22.686204567889387	27.515425866991066	28.24880290087135	21.549566664248193
16	22.65220671859002	27.43314617472885	28.247855915659986	21.66679119102114
17	22.697781773265486	27.50147102827857	28.17034909007281	21.63039810838314
18	22.54590672843795	27.444131923321564	28.285476073410436	21.72448527483005
19	22.297764196721594	27.6623273979925	28.426947742439467	21.61296066284644
20	22.187139085855446	27.66416527681226	28.460802288850573	21.687893348481722
21	22.336086491796063	27.639416117004163	28.272056248761135	21.752441142438638
22	22.268123199535363	27.677219910670132	28.355686205107993	21.69897068468651
23	22.36850723264456	27.722797624759448	28.180497541709208	21.72819760088678
24	22.22954165375664	27.696289240249605	28.19838944100687	21.875779664986876
25	22.274053559395146	27.800677256457817	28.14624764311404	21.779021541032996
26	22.324913925149986	27.756825676194374	28.05731169933826	21.86094869931738
27	22.32035941942819	27.706905113950768	27.999786406217638	21.972949060403398
28	22.307597719962068	27.786346453639403	28.01919679450907	21.886859031889458
29	22.38104488475856	27.77205525856747	27.976197184645375	21.870702672028592
30	22.347990419966337	27.706061551518246	27.99559777901362	21.9503502495018
31	22.450869741103965	27.68813724588255	27.882211602945304	21.978781410068184
32	22.463792668570118	27.787592297301504	27.786994580400037	21.96162045372834
33	22.42840494743962	27.725753802928775	27.770193694482764	22.075647555148837
34	22.475871293014084	27.72436647605868	27.797200178427627	22.00256205249961
35	22.50277699971255	27.714736190518472	27.732412344155755	22.05007446561322
36	22.519469121601006	27.743704872093648	27.670394444308215	22.066431561997128
37	22.46741137631698	27.708942114898832	27.754001327579648	22.06964518120454
38	22.464368781246236	27.763276741665134	27.78439127124483	21.9879632058438
39	22.443344269272185	27.60006762122536	27.789432257160858	22.1671558523416
40	22.551023329142712	27.80341019096515	27.63781020291949	22.00775627697265
41	22.49029842012873	27.630974479002568	27.782476481973262	22.09625061889544
42	22.440463675084487	27.65738272466673	27.73997376430953	22.162179835939252
43	22.60566692676961	27.726982428594322	27.58663773956077	22.08071290507529
44	22.558923734910348	27.607439632382437	27.67432272541673	22.15931390729049
45	22.591001948161022	27.5627823289679	27.65552566771009	22.19069005516099
46	22.46986705659893	27.645144837427303	27.698698111376626	22.186289994597143
47	22.481613300065547	27.69827191433813	27.658808761576122	22.1613060240202
48	22.494081792516983	27.5844009571392	27.659731290245748	22.261785960098077
49	22.606833555274804	27.64550206919929	27.588560516171178	22.159103859354722
50	22.47474202771815	27.64011497836116	27.624547677730014	22.260595316190674
51	22.501695891383335	27.61524516299109	27.59247822907437	22.290580716551204
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	43.45196291488663
2	41.443458588436556
3	41.42633735668607
4	42.117013902610864
5	44.65093963077298
6	43.12699465590469
7	44.869318741359436
8	42.58119723055435
9	42.23000614460176
10	42.53574554111293
11	45.11912707895419
12	42.65219951718157
13	44.34576848252705
14	44.07705579057152
15	44.23577123213758
16	44.31899790961116
17	44.32817988164862
18	44.270392003268
19	43.910724859568035
20	43.875032434337164
21	44.0885276342347
22	43.967093884221875
23	44.09670483353134
24	44.10532131874352
25	44.05307510042814
26	44.18586262446736
27	44.29330847983159
28	44.19445675185153
29	44.25174755678716
30	44.29834066946813
31	44.429651151172145
32	44.42541312229846
33	44.50405250258845
34	44.47843334551369
35	44.55285146532577
36	44.58590068359813
37	44.537056557521524
38	44.452331987090034
39	44.61050012161378
40	44.558779606115365
41	44.58654903902417
42	44.602643511023736
43	44.6863798318449
44	44.71823764220083
45	44.78169200332201
46	44.65615705119607
47	44.64291932408575
48	44.75586775261506
49	44.76593741462953
50	44.735337343908824
51	44.79227660793454
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14871.0
1	9235.0
2	3599.0
3	5059.0
4	6519.0
5	8970.5
6	11422.0
7	18449.5
8	25477.0
9	21554.0
10	17631.0
11	20635.5
12	23640.0
13	31768.0
14	39896.0
15	56757.0
16	73618.0
17	108066.5
18	142515.0
19	180531.5
20	218548.0
21	260651.0
22	302754.0
23	358563.5
24	414373.0
25	491839.0
26	667343.5
27	765382.0
28	829660.5
29	893939.0
30	953668.5
31	1013398.0
32	1091539.5
33	1169681.0
34	1244005.5
35	1318330.0
36	1441192.5
37	1564055.0
38	1631975.0
39	1699895.0
40	1774327.0
41	1848759.0
42	1858741.0
43	1868723.0
44	1877241.0
45	1885759.0
46	1857786.5
47	1829814.0
48	1789840.5
49	1749867.0
50	1672992.0
51	1596117.0
52	1490828.0
53	1385539.0
54	1287450.5
55	1189362.0
56	1081267.5
57	973173.0
58	863557.5
59	753942.0
60	673085.5
61	592229.0
62	513000.5
63	433772.0
64	375795.5
65	317819.0
66	266962.0
67	216105.0
68	206175.5
69	196246.0
70	176508.0
71	156770.0
72	134671.0
73	112572.0
74	97153.0
75	68474.0
76	55214.0
77	52868.5
78	50523.0
79	50838.5
80	51154.0
81	44738.5
82	38323.0
83	34712.0
84	31101.0
85	28416.5
86	25732.0
87	29409.5
88	33087.0
89	20537.5
90	7988.0
91	4686.5
92	1385.0
93	968.0
94	551.0
95	321.0
96	91.0
97	61.5
98	32.0
99	23.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03384301901765947
2	3.240633803159222E-4
3	0.004122806338463677
4	0.04949528028691852
5	0.0
6	3.960774648305715E-5
7	0.0
8	0.0
9	0.0
10	0.0
11	1.0081971832050912E-4
12	0.0
13	2.664521127042027E-4
14	0.0
15	0.0
16	0.0
17	0.01567746619883917
18	0.0
19	0.0
20	0.003064199296098331
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	1.8003521128662345E-5
27	3.6007042257324684E-6
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0031002063383556554
35	0.0016779281691913304
36	1.2242394367490396E-4
37	0.0
38	0.0
39	0.0
40	0.0
41	0.003942771127177053
42	0.006402052113352329
43	2.8805633805859747E-5
44	1.8003521128662345E-5
45	0.0
46	0.0
47	0.007691104226164553
48	0.0
49	2.8805633805859747E-5
50	0.009354629578452954
51	0.14093876480362028
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.7772345E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	27.994448424875735
#Duplication Level	Relative count
1	100.0
2	9.945304579646294
3	2.518242236879983
4	1.1984179631275267
5	0.7472120354957007
6	0.4962639398225215
7	0.40176727536703505
8	0.31916091968409327
9	0.2540771242975331
10++	9.280698899833535
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	7804365	3.2806737	3.9328227	16
TGTGT	4358285	2.93924	25.83993	4
TGTTT	4141545	2.2051334	20.49887	6
CTTTG	2869630	1.993499	25.913061	1
GTTTG	2921785	1.9704605	25.052814	7
GAGGG	1748820	1.9412494	7.5683837	11
TTTGA	3417965	1.86708	20.56921	8
TTTGT	3456895	1.8405967	20.206415	2
GTGTT	2605665	1.7572681	24.98326	5
TTGTG	2462620	1.660798	24.985441	3
TGAGG	1885120	1.6520692	9.596191	10
TTGAG	2194665	1.5184851	15.573327	9
>>END_MODULE