FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_B_13_GTCCGC_L008_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_B_13_GTCCGC_L008_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 29798025
Filtered Sequences 0
Sequence length 51
%GC 45

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 47719 0.160141485887068 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 12975705 6.1009755 6.8681703 18
AAAAA 7312425 3.5415475 3.9487433 44
GGGGG 3363520 3.4796202 4.6361713 17
CCCCC 2367850 3.1273148 4.325477 47
TGTGT 4157145 2.6794848 14.30005 7
TGTTT 4420485 2.4335046 12.63758 9
GTTTG 3496130 2.2534285 14.158863 10
TTTGA 3967075 2.1968746 12.40344 11
GTGTT 3148045 2.0290701 13.897725 8
CTTTG 2930455 1.9833852 14.34294 4
AACTT 3151485 1.8434812 12.99853 2
ACTTT 3128985 1.8195093 12.435935 3
TTTGT 3228340 1.7772212 11.826943 5
TTGTG 2587080 1.6675007 13.636445 6
TTGAG 2425105 1.5723866 8.33955 12
TGAGG 1957100 1.4857156 5.091981 13
TAACT 2186395 1.2789457 12.347559 1
GTAAC 1166120 0.79865754 5.6061444 1
Produced by FastQC (version 0.10.1)