##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_B_13_GTCCGC_L008_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	29798025	
Filtered Sequences	0	
Sequence length	51	
%GC	45	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.283067955007084	33.0	31.0	34.0	31.0	34.0
2	32.41673986782681	34.0	31.0	34.0	31.0	34.0
3	32.497412294942364	34.0	31.0	34.0	31.0	34.0
4	35.914147833623204	37.0	37.0	37.0	35.0	37.0
5	35.94850873505878	37.0	37.0	37.0	35.0	37.0
6	36.00444257631168	37.0	37.0	37.0	35.0	37.0
7	35.974968072548435	37.0	37.0	37.0	35.0	37.0
8	35.98492087982341	37.0	37.0	37.0	35.0	37.0
9	37.710616928470934	39.0	38.0	39.0	35.0	39.0
10	37.689739001158635	39.0	38.0	39.0	35.0	39.0
11	37.663358695752486	39.0	38.0	39.0	35.0	39.0
12	37.62389034172567	39.0	38.0	39.0	35.0	39.0
13	37.60772896861453	39.0	38.0	39.0	35.0	39.0
14	39.121501139756745	41.0	40.0	41.0	36.0	41.0
15	39.07569525161483	41.0	40.0	41.0	36.0	41.0
16	39.035486177355715	41.0	40.0	41.0	36.0	41.0
17	38.982362421670565	41.0	39.0	41.0	36.0	41.0
18	38.94492903472629	41.0	39.0	41.0	36.0	41.0
19	38.94963172894848	41.0	39.0	41.0	36.0	41.0
20	38.90634577291616	41.0	39.0	41.0	36.0	41.0
21	38.85539414105465	41.0	39.0	41.0	36.0	41.0
22	38.77666570183762	41.0	39.0	41.0	35.0	41.0
23	38.73581484007749	41.0	39.0	41.0	35.0	41.0
24	38.676573766214375	41.0	39.0	41.0	35.0	41.0
25	38.63317286967845	41.0	39.0	41.0	35.0	41.0
26	38.564356563899786	41.0	39.0	41.0	35.0	41.0
27	38.4951195590983	40.0	39.0	41.0	35.0	41.0
28	38.4310735023546	40.0	39.0	41.0	34.0	41.0
29	38.33651851087446	40.0	38.0	41.0	34.0	41.0
30	38.27436851939013	40.0	38.0	41.0	34.0	41.0
31	38.22889772057041	40.0	38.0	41.0	34.0	41.0
32	38.13840205852569	40.0	38.0	41.0	34.0	41.0
33	38.06742366314546	40.0	38.0	41.0	33.0	41.0
34	38.00845039226593	40.0	38.0	41.0	33.0	41.0
35	37.92652774806384	40.0	38.0	41.0	33.0	41.0
36	37.85114493326319	40.0	38.0	41.0	33.0	41.0
37	37.77593786836544	40.0	38.0	41.0	33.0	41.0
38	37.67405416969749	40.0	38.0	41.0	33.0	41.0
39	37.59112347210931	40.0	38.0	41.0	32.0	41.0
40	37.48826118509532	40.0	37.0	41.0	32.0	41.0
41	37.69569291924549	40.0	38.0	41.0	33.0	41.0
42	37.720919792503025	40.0	38.0	41.0	33.0	41.0
43	37.68737662982698	40.0	38.0	41.0	33.0	41.0
44	37.634293917130414	40.0	37.0	41.0	33.0	41.0
45	37.58353961378313	40.0	37.0	41.0	33.0	41.0
46	37.509623775401224	40.0	37.0	41.0	32.0	41.0
47	37.4355889358439	40.0	37.0	41.0	32.0	41.0
48	37.33184867117871	40.0	37.0	41.0	32.0	41.0
49	37.25981926654535	40.0	37.0	41.0	32.0	41.0
50	37.13807203665343	40.0	36.0	41.0	31.0	41.0
51	35.63655826183111	38.0	35.0	39.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	6.0
4	65.0
5	230.0
6	1711.0
7	8793.0
8	26972.0
9	37017.0
10	38916.0
11	39536.0
12	43009.0
13	49029.0
14	53480.0
15	56198.0
16	56730.0
17	56574.0
18	56511.0
19	56993.0
20	58026.0
21	60921.0
22	65665.0
23	71979.0
24	80989.0
25	91587.0
26	105460.0
27	122072.0
28	144627.0
29	173377.0
30	210382.0
31	262251.0
32	337777.0
33	454667.0
34	663940.0
35	1030180.0
36	1549266.0
37	2534904.0
38	5639181.0
39	1.5555164E7
40	3839.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	28.468613450052853	34.936568750231785	18.18529520002	18.409522599695357
2	25.808611901625884	32.34116369449929	22.795277613399108	19.054946790475714
3	23.425596100249386	28.778772728700524	26.28167292256583	21.51395824848426
4	22.097592467570813	26.7441984167205	28.86666925522718	22.29153986048151
5	22.599780441817217	27.605085491574876	28.9560878264948	20.839046240113102
6	21.962800018739532	26.067102821628264	29.456699267990455	22.513397891641755
7	22.958187097361535	26.097847340253118	30.091938999693586	20.852026562691762
8	23.642825674546938	26.640985111639043	27.67978984865013	22.03639936516388
9	23.525812964269964	26.84523378568381	28.180857083101184	21.448096166945042
10	24.04599849436063	27.560678612452865	27.229778967589485	21.16354392559702
11	23.125244540690446	26.042846365193494	29.29419997563865	21.5377091184774
12	22.56946417505438	26.34665279705247	29.604868712535453	21.479014315357688
13	22.89922326590808	26.268534784543725	29.23683240708358	21.59540954246462
14	23.832185439571642	26.535940315900923	27.966804564270053	21.66506968025738
15	23.10641921145298	27.503117914212723	27.510340466834656	21.88012240749964
16	23.748895922693034	26.815293120858076	27.516191678967022	21.91961927748187
17	23.47006998853354	26.820004769161347	27.615605595390864	22.09431964691425
18	23.439415169228532	26.448668174506146	27.634330612606135	22.477586043659194
19	23.47573332307581	26.61146400994147	27.627452151225018	22.2853505157577
20	23.297439737050265	26.799112537935443	27.54779035571308	22.35565736930121
21	23.277782064236085	26.754649402371317	27.543744052818774	22.423824480573824
22	23.156654179583388	26.881839613592472	27.736467279168064	22.225038927656072
23	23.046074386247188	26.939484331603254	27.67818989797658	22.33625138417298
24	23.00381886480265	26.98296921942591	27.66569118968349	22.347520726087943
25	23.01408809771514	27.042933924330736	27.642104819431495	22.30087315852263
26	23.0106043256927	27.0956107900339	27.60736400187178	22.28642088240162
27	22.938106628862215	26.85787371991555	27.598730278727235	22.605289372495005
28	23.04371235281451	26.99495513618098	27.512702107356336	22.448630403648178
29	22.97764016709421	27.195042656848976	27.357196449886178	22.470120726170638
30	23.039214550527237	27.039921850474496	27.312207481993724	22.60865611700455
31	23.149439643631986	27.054260698304493	27.226517949047086	22.56978170901643
32	23.183196249166357	27.074664331376237	27.17861094199056	22.56352847746685
33	23.124842374045848	26.96225325632185	27.098454836809367	22.814449532822938
34	23.277744387394332	26.881962129934067	27.158003473495064	22.682290009176537
35	23.199988400176977	26.98263161452553	27.101086751706525	22.716293233590967
36	23.2465052008103	26.903282096360954	27.083127544668095	22.76708515816065
37	23.154014156812426	26.94180562627398	27.078644519949535	22.82553569696406
38	23.262123112098184	26.96818370289793	27.108408412945423	22.661284772058465
39	23.118043853799904	26.8409995818416	27.20857820169601	22.83237836266249
40	23.330732569050898	26.764224931000058	27.073593548322762	22.83144895162629
41	23.208510016702615	26.913478623692633	27.139256960827428	22.73875439877732
42	23.147451940179366	26.772368507564842	27.130059359282495	22.950120192973298
43	23.378937427635517	26.837606676915605	27.018031280558763	22.76542461489011
44	23.343166934128526	26.936108894849394	26.897632652074805	22.823091518947276
45	23.23956551711589	26.928331192449694	26.939302507697544	22.89280078273687
46	23.20622881539147	26.99650626007616	26.89570102674434	22.901563897788034
47	23.17337715843263	26.847804010697423	26.96786587897757	23.01095295189238
48	23.264619019373033	26.738510331120874	26.921748576296622	23.075122073209474
49	23.23882562282836	26.967115597088558	26.916303291662636	22.877755488420448
50	23.07585313799716	26.889555227525353	27.134056296310625	22.900535338166858
51	23.256464046403835	26.845005697319284	26.89327917939785	23.005251076879034
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	46.87813604974821
2	44.8635586921016
3	44.939554348733644
4	44.38913232805232
5	43.43882668193032
6	44.47619791038128
7	43.8102136600533
8	45.67922503971082
9	44.973909131215
10	45.20954241995766
11	44.66295365916785
12	44.04847849041207
13	44.4946328083727
14	45.49725511982902
15	44.98654161895262
16	45.66851520017491
17	45.56438963544779
18	45.91700121288772
19	45.761083838833514
20	45.653097106351474
21	45.701606544809906
22	45.381693107239464
23	45.38232577042017
24	45.351339590890596
25	45.314961256237765
26	45.29702520809432
27	45.54339600135722
28	45.49234275646268
29	45.44776089326484
30	45.647870667531784
31	45.71922135264842
32	45.746724726633204
33	45.93929190686879
34	45.96003439657087
35	45.916281633767944
36	46.01359035897095
37	45.97954985377648
38	45.923407884156646
39	45.950422216462385
40	46.16218152067719
41	45.947264415479935
42	46.09757213315267
43	46.144362042525636
44	46.1662584530758
45	46.13236629985276
46	46.1077927131795
47	46.18433011032501
48	46.33974109258251
49	46.116581111248806
50	45.97638847616402
51	46.26171512328286
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	61865.0
1	36877.0
2	11889.0
3	12544.5
4	13200.0
5	24954.5
6	36709.0
7	37478.0
8	38247.0
9	33046.0
10	27845.0
11	29418.0
12	30991.0
13	35979.0
14	40967.0
15	56991.0
16	73015.0
17	108621.5
18	144228.0
19	181789.0
20	219350.0
21	253546.5
22	287743.0
23	336978.0
24	386213.0
25	462089.0
26	649611.0
27	761257.0
28	819383.5
29	877510.0
30	916194.0
31	954878.0
32	1024296.0
33	1093714.0
34	1157192.5
35	1220671.0
36	1385495.5
37	1550320.0
38	1618610.0
39	1686900.0
40	1797710.5
41	1908521.0
42	1895814.0
43	1883107.0
44	1885050.0
45	1886993.0
46	1872132.0
47	1857271.0
48	1836000.0
49	1814729.0
50	1755788.0
51	1696847.0
52	1602985.5
53	1509124.0
54	1422269.0
55	1335414.0
56	1250814.5
57	1166215.0
58	1057531.5
59	948848.0
60	863036.0
61	777224.0
62	690481.5
63	603739.0
64	541250.0
65	478761.0
66	404476.0
67	330191.0
68	337004.0
69	343817.0
70	306131.5
71	268446.0
72	234007.0
73	199568.0
74	170955.5
75	122097.5
76	101852.0
77	97822.5
78	93793.0
79	95889.5
80	97986.0
81	83870.0
82	69754.0
83	66007.0
84	62260.0
85	55766.5
86	49273.0
87	58930.0
88	68587.0
89	47669.5
90	26752.0
91	18243.0
92	9734.0
93	7648.5
94	5563.0
95	4433.0
96	3303.0
97	2563.5
98	1824.0
99	1266.5
100	709.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007987106528033317
2	0.0021276577894004723
3	5.7050760914523696E-5
4	8.524054866052363E-4
5	3.3559271126190413E-6
6	1.7786413696880917E-4
7	0.027763585002697325
8	0.01301764126984926
9	0.018182413096169966
10	0.01577956928353473
11	0.016531296956761397
12	0.014625130356793782
13	0.013574725170544021
14	0.0042888748499271346
15	0.008272360332605935
16	0.04353644243200682
17	0.008154902883664269
18	0.007356192230860938
19	0.017638752903925678
20	0.0023323693432702334
21	0.004517077893585229
22	0.02519630076154376
23	0.01665882218704092
24	0.04800653734601538
25	0.008453580396687364
26	0.020558409491904246
27	0.0655110531654363
28	0.009963747597365933
29	0.07787428864832485
30	0.05760784481521846
31	0.025830570985828756
32	0.0388616359641285
33	0.028042126953044706
34	0.03023354735758494
35	0.014990926412069257
36	0.013792860432864257
37	0.015420485082484494
38	0.04354315428623206
39	0.01841397206694068
40	0.05286927573220037
41	0.01707160122189306
42	0.010141611734334742
43	0.040861768523249443
44	0.018843530737355917
45	0.03778438336097778
46	0.033186763216689695
47	0.016954143772951395
48	0.05197324319313109
49	0.020232884561980198
50	0.08851593352243982
51	0.12891794003126045
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.9798025E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	42.61089218955239
#Duplication Level	Relative count
1	100.0
2	13.931476460438748
3	4.803690270784182
4	2.4240290151061656
5	1.593718088665094
6	1.0591922250783479
7	0.8155216732983556
8	0.6641078911229269
9	0.547906616430156
10++	15.010387689707384
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	47719	0.160141485887068	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	12975705	6.1009755	6.8681703	18
AAAAA	7312425	3.5415475	3.9487433	44
GGGGG	3363520	3.4796202	4.6361713	17
CCCCC	2367850	3.1273148	4.325477	47
TGTGT	4157145	2.6794848	14.30005	7
TGTTT	4420485	2.4335046	12.63758	9
GTTTG	3496130	2.2534285	14.158863	10
TTTGA	3967075	2.1968746	12.40344	11
GTGTT	3148045	2.0290701	13.897725	8
CTTTG	2930455	1.9833852	14.34294	4
AACTT	3151485	1.8434812	12.99853	2
ACTTT	3128985	1.8195093	12.435935	3
TTTGT	3228340	1.7772212	11.826943	5
TTGTG	2587080	1.6675007	13.636445	6
TTGAG	2425105	1.5723866	8.33955	12
TGAGG	1957100	1.4857156	5.091981	13
TAACT	2186395	1.2789457	12.347559	1
GTAAC	1166120	0.79865754	5.6061444	1
>>END_MODULE