FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_B_13_GTCCGC_L008_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_B_13_GTCCGC_L008_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 29798025
Filtered Sequences 0
Sequence length 51
%GC 45

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 71486 0.23990180557268478 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 12005430 5.7775874 6.553178 18
AAAAA 6626160 3.079485 3.3989556 1
TGTGT 3086280 2.074303 5.9998064 1
TGTTT 3436835 1.9546162 5.656568 3
TTTGA 3038680 1.7161578 5.3843913 5
GTTTG 2521360 1.6946175 5.9244437 4
CTTTG 2446345 1.6848799 5.214897 1
GTGTT 2164815 1.454982 6.036843 2
TTGTG 1775725 1.1934727 5.2137885 3
Produced by FastQC (version 0.10.1)