##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_B_13_GTCCGC_L008_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	29798025	
Filtered Sequences	0	
Sequence length	51	
%GC	45	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.67281281225853	34.0	31.0	34.0	31.0	34.0
2	32.920215886791155	34.0	31.0	34.0	31.0	34.0
3	33.01342904437458	34.0	31.0	34.0	31.0	34.0
4	36.45571654497236	37.0	37.0	37.0	35.0	37.0
5	36.38685926332366	37.0	37.0	37.0	35.0	37.0
6	36.40377259902292	37.0	37.0	37.0	35.0	37.0
7	36.394113636725926	37.0	37.0	37.0	35.0	37.0
8	36.386857887393546	37.0	37.0	37.0	35.0	37.0
9	38.17473399663233	39.0	39.0	39.0	37.0	39.0
10	38.165479759145114	39.0	39.0	39.0	37.0	39.0
11	38.158739748691396	39.0	39.0	39.0	37.0	39.0
12	38.13183216001732	39.0	38.0	39.0	37.0	39.0
13	38.10234946779191	39.0	38.0	39.0	37.0	39.0
14	39.66237517419359	41.0	40.0	41.0	37.0	41.0
15	39.6599292738361	41.0	40.0	41.0	37.0	41.0
16	39.63516709580585	41.0	40.0	41.0	37.0	41.0
17	39.604770987338924	41.0	40.0	41.0	37.0	41.0
18	39.587219991929	41.0	40.0	41.0	37.0	41.0
19	39.59122492178592	41.0	40.0	41.0	37.0	41.0
20	39.564949925372574	41.0	40.0	41.0	37.0	41.0
21	39.52856590327715	41.0	40.0	41.0	37.0	41.0
22	39.4847005799881	41.0	39.0	41.0	37.0	41.0
23	39.43901530386662	41.0	39.0	41.0	37.0	41.0
24	39.40008816020525	41.0	39.0	41.0	36.0	41.0
25	39.34029463362085	41.0	39.0	41.0	36.0	41.0
26	39.26443497513677	41.0	39.0	41.0	36.0	41.0
27	39.17945343693081	40.0	39.0	41.0	36.0	41.0
28	39.10475516414259	40.0	39.0	41.0	36.0	41.0
29	39.03828351040044	40.0	39.0	41.0	35.0	41.0
30	38.971349913291235	40.0	39.0	41.0	35.0	41.0
31	38.890142584953196	40.0	38.0	41.0	35.0	41.0
32	38.79051151880032	40.0	38.0	41.0	35.0	41.0
33	38.7236294351723	40.0	38.0	41.0	35.0	41.0
34	38.87257561533021	40.0	39.0	41.0	35.0	41.0
35	38.89037152630082	40.0	39.0	41.0	35.0	41.0
36	38.85863220800707	40.0	38.0	41.0	35.0	41.0
37	38.820452731347125	40.0	38.0	41.0	35.0	41.0
38	38.7728632350634	40.0	38.0	41.0	35.0	41.0
39	38.695066166297934	40.0	38.0	41.0	35.0	41.0
40	38.50720233975238	40.0	38.0	41.0	35.0	41.0
41	38.502715935032604	40.0	38.0	41.0	35.0	41.0
42	38.326489456935484	40.0	38.0	41.0	34.0	41.0
43	38.27484808137452	40.0	38.0	41.0	34.0	41.0
44	38.28901767818505	40.0	38.0	41.0	34.0	41.0
45	38.26408837498458	40.0	38.0	41.0	34.0	41.0
46	38.17985051022677	40.0	38.0	41.0	34.0	41.0
47	38.11073495642748	40.0	38.0	41.0	34.0	41.0
48	38.0501483907071	40.0	37.0	41.0	34.0	41.0
49	37.97953354291098	40.0	37.0	41.0	33.0	41.0
50	38.05515993090146	40.0	37.0	41.0	34.0	41.0
51	37.75000628397352	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	4.0
8	9.0
9	13.0
10	21.0
11	44.0
12	73.0
13	163.0
14	337.0
15	556.0
16	831.0
17	1385.0
18	2319.0
19	3914.0
20	7027.0
21	11714.0
22	18426.0
23	27847.0
24	39731.0
25	54278.0
26	71823.0
27	93869.0
28	121079.0
29	154170.0
30	197206.0
31	252322.0
32	327022.0
33	437857.0
34	646306.0
35	1038234.0
36	1566146.0
37	2600252.0
38	5565650.0
39	1.6545306E7
40	12090.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	27.98580055965975	36.79113191210257	15.995232167495796	19.227835360741878
2	24.12740318850953	31.38701654662526	23.536074977575296	20.949505287289913
3	20.908277452672852	27.49912456260852	28.84735184300295	22.74524614171568
4	20.49226335733122	27.83766721095466	30.131635832426795	21.53843359928733
5	22.454202921166754	28.527924250013214	28.185156566584528	20.8327162622355
6	22.767196492373582	27.052942612044404	27.241777423265972	22.93808347231604
7	23.3026215663622	27.22027718279987	28.101791981179964	21.375309269657972
8	22.68843992177334	27.508172102010114	27.04804429152603	22.755343684690512
9	22.537490320247734	27.361024765903107	27.929777225168444	22.17170768868071
10	23.045654871421846	27.524465128141884	27.606054428103878	21.823825572332396
11	22.736907598863144	26.84787004232833	28.375384799186254	22.03983755962227
12	22.578442698803023	27.163189506687104	28.230709921211222	22.02765787329865
13	22.60901183887187	27.218763659672074	28.159433385266308	22.01279111618975
14	22.568552633204046	27.092117571501372	28.217152274388273	22.12217752090631
15	22.76657932866356	26.937385950914532	28.04181149589612	22.254223224525788
16	23.03900677981175	27.070159851198195	27.679532452234668	22.211300916755388
17	23.00572132621359	27.19970152287235	27.47695138785689	22.317625763057165
18	22.86716317608298	26.849343203114973	27.703416585495177	22.58007703530687
19	22.952138606501606	27.03567434418892	27.580824568071204	22.43136248123827
20	22.863226673579877	27.146289057747953	27.53680151620787	22.4536827524643
21	22.920639874622566	27.00578310139682	27.47239120713537	22.60118581684524
22	22.922562820858094	27.074032591086155	27.55668538435014	22.446719203705616
23	22.90805514795024	27.058622173785007	27.50922921905059	22.52409345921416
24	22.808135656835095	27.06279533010457	27.531131942627585	22.597937070432753
25	22.906608743364703	27.096305879332604	27.54056686642823	22.456518510874464
26	22.925036139140094	27.08187203682123	27.41945481286092	22.573637011177755
27	22.891033885635036	26.86011572914648	27.40211809339713	22.846732291821354
28	22.97726107686667	27.064458131033852	27.415363937710634	22.54291685438884
29	22.963770249873942	27.19952412953543	27.268448160574398	22.568257460016223
30	22.94689329242458	27.006286490463715	27.328713899662816	22.718106317448893
31	23.067384499476056	27.02751944130525	27.201799448117786	22.703296611100903
32	23.103320287723818	27.12830919638285	27.14765611943344	22.6207143964599
33	22.990510277107294	26.969720308644618	27.062659354101488	22.977110060146604
34	23.10597536036141	26.98501396167434	27.162354710896388	22.74665596706786
35	23.01313332624785	27.05738648126548	27.16189681250873	22.767583379977946
36	23.11007524827568	26.970250545128412	26.983271542325372	22.936402664270535
37	23.057642243068123	26.9932520695583	27.010051840684074	22.939053846689504
38	23.232499469344027	26.9921345458298	27.062679489664166	22.712686495162014
39	23.020203520199743	26.879398886335586	27.162112254083954	22.938285339380716
40	23.257061499881285	26.97578446893712	27.00610191447252	22.761052116709077
41	23.107402932885773	26.966895072380687	27.192720866356396	22.732981128377148
42	23.138780842454338	26.93166710338666	26.993164750468225	22.936387303690786
43	23.27735299754618	26.97938047769142	27.01328205565853	22.729984469103872
44	23.266819289793034	26.930848219502384	26.887905766081488	22.914426724623095
45	23.21034267759299	26.929232083928827	26.919946228622187	22.940479009855995
46	23.09571053704045	27.020266202301034	27.033412235261565	22.850611025396947
47	23.069592171877062	26.99630013004605	26.99988800070724	22.934219697369652
48	23.166603760527437	26.809043743674003	27.0556228321966	22.968729663601952
49	23.232012859912697	26.99493003311461	27.019357826567365	22.75369928040533
50	22.997005249380038	26.891422813124414	27.17029812020356	22.94127381729199
51	23.19241928513691	26.93020935303491	26.868219220614787	23.009152141213388
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	47.213635920401636
2	45.07690847579944
3	43.653523594388524
4	42.03069695661855
5	43.286919183402254
6	45.70527996468962
7	44.677930836020174
8	45.44378360646385
9	44.70919800892844
10	44.86948044375424
11	44.77674515848541
12	44.606100572101674
13	44.62180295506162
14	44.690730154110355
15	45.02080255318935
16	45.25030769656714
17	45.32334708927076
18	45.44724021138985
19	45.38350108773987
20	45.31690942604418
21	45.521825691467804
22	45.36928202456371
23	45.4321486071644
24	45.40607272726784
25	45.36312725423917
26	45.49867315031785
27	45.73776617745639
28	45.52017793125551
29	45.53202770989017
30	45.664999609873476
31	45.77068111057696
32	45.724034684183714
33	45.9676203372539
34	45.852631327429265
35	45.780716706225796
36	46.046477912546216
37	45.99669608975763
38	45.94518596450604
39	45.95848885958046
40	46.01811361659036
41	45.84038406126292
42	46.075168146145124
43	46.00733746665005
44	46.181246014416125
45	46.15082168744898
46	45.9463215624374
47	46.00381186924671
48	46.135333424129385
49	45.98571214031802
50	45.938279066672024
51	46.2015714263503
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	87273.0
1	49282.5
2	11292.0
3	11815.5
4	12339.0
5	17299.5
6	22260.0
7	23108.0
8	23956.0
9	21591.0
10	19226.0
11	22591.5
12	25957.0
13	31297.5
14	36638.0
15	54494.0
16	72350.0
17	110345.5
18	148341.0
19	187885.5
20	227430.0
21	259728.0
22	292026.0
23	340266.0
24	388506.0
25	470929.5
26	670320.5
27	787288.0
28	838486.0
29	889684.0
30	925881.5
31	962079.0
32	1026767.0
33	1091455.0
34	1156758.0
35	1222061.0
36	1390376.0
37	1558691.0
38	1617689.5
39	1676688.0
40	1789695.0
41	1902702.0
42	1884113.5
43	1865525.0
44	1864845.0
45	1864165.0
46	1848206.5
47	1832248.0
48	1819948.0
49	1807648.0
50	1747447.5
51	1687247.0
52	1603260.5
53	1519274.0
54	1437100.0
55	1354926.0
56	1278568.5
57	1202211.0
58	1080155.5
59	958100.0
60	871328.5
61	784557.0
62	696469.5
63	608382.0
64	545239.5
65	482097.0
66	406759.0
67	331421.0
68	340857.5
69	350294.0
70	310786.5
71	271279.0
72	235285.5
73	199292.0
74	169268.0
75	117115.0
76	94986.0
77	93123.0
78	91260.0
79	93545.5
80	95831.0
81	81755.0
82	67679.0
83	61064.0
84	54449.0
85	49042.0
86	43635.0
87	52772.5
88	61910.0
89	38574.5
90	15239.0
91	8737.0
92	2235.0
93	1665.0
94	1095.0
95	621.0
96	147.0
97	97.0
98	47.0
99	27.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.022518270925673767
2	0.0017752854425754725
3	0.0050036873249149905
4	0.026968230277006615
5	0.0
6	0.0018558276932783295
7	0.0
8	0.0
9	0.0
10	0.0
11	5.0338906689285614E-5
12	0.0
13	0.0
14	0.007493785242478319
15	0.0
16	0.0
17	0.004822467260833562
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	4.362705246404754E-5
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	1.6779635563095205E-5
33	0.0
34	7.718632359023794E-5
35	4.698297957666657E-5
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.012819641570204737
42	4.5976201442880863E-4
43	6.711854225238083E-6
44	2.3491489788333286E-5
45	5.369483380190466E-5
46	0.0065910408491837965
47	0.010718831197705217
48	0.007755547557262604
49	0.0
50	0.00521511073300999
51	0.06407136043412273
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.9798025E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	44.971653543307085
#Duplication Level	Relative count
1	100.0
2	15.203331139283977
3	5.099733852273703
4	2.6550382050768393
5	1.5961709069055319
6	1.1604613227255816
7	0.8893054402884698
8	0.6582148069713534
9	0.557336233294222
10++	15.270583521735398
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	71486	0.23990180557268478	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	12005430	5.7775874	6.553178	18
AAAAA	6626160	3.079485	3.3989556	1
TGTGT	3086280	2.074303	5.9998064	1
TGTTT	3436835	1.9546162	5.656568	3
TTTGA	3038680	1.7161578	5.3843913	5
GTTTG	2521360	1.6946175	5.9244437	4
CTTTG	2446345	1.6848799	5.214897	1
GTGTT	2164815	1.454982	6.036843	2
TTGTG	1775725	1.1934727	5.2137885	3
>>END_MODULE