FastQCFastQC Report
Mon 11 Aug 2014
140701_JD_B_12_GGCTAC_L002_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_B_12_GGCTAC_L002_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 35734689
Filtered Sequences 0
Sequence length 51
%GC 45

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[WARN] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 86327 0.2415775886562214 No Hit
TTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 83891 0.23476068309983053 No Hit
TAACTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 43661 0.12218099897273486 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGGGG 13133545 9.378768 18.957727 43
TTTTT 24621550 7.489593 10.153844 18
AAAAA 9251810 4.452207 4.933497 47
TGTGT 10402190 4.4515986 38.244446 4
GTTTG 7808530 3.341647 37.610886 7
TGTTT 9191235 3.3162014 32.22524 6
TTTGA 8244430 3.2603848 34.95876 8
GTGTT 7573140 3.240912 37.700966 5
TTGTG 6939395 2.969702 37.788467 3
CTTTG 5810215 2.8918464 44.121418 1
GAGGG 4050585 2.672998 12.207174 9
TTTGT 6579330 2.373825 32.130455 2
TTGAG 5022685 2.3559623 23.676779 9
GGGGA 3541565 2.3370934 8.619806 22
AGGGG 3504825 2.3128486 10.760539 10
GGGAG 3407825 2.248838 5.294396 23
GGGGC 2528425 2.0999343 8.501048 45
TGAGG 3553460 1.9770063 13.445593 10
GGCCC 1681040 1.8885009 5.016051 46
ACTTT 4087920 1.8801928 22.253134 3
AACTT 3655280 1.8427303 24.994726 2
GGGCC 1876730 1.8127972 6.4240713 45
GGAGA 2885210 1.7594428 5.0336328 6
GGGAA 2874335 1.7528113 5.8199253 22
CGGGG 1933395 1.6057439 7.159655 40
TGAGC 2232785 1.4447585 5.6245155 10
TAACT 2835440 1.4294257 24.772512 1
GGCGG 1563345 1.2984059 7.3870535 13
GGGCG 1561655 1.2970023 6.4429255 12
TTGAT 3223950 1.2749598 8.64355 9
TGAGT 2580135 1.2102494 6.546221 10
GAGTG 2105760 1.1715626 5.039163 11
GCGGG 1375270 1.1422039 6.6181574 14
TTGAC 1984330 1.0825254 7.0659914 9
GTAAC 1284085 0.7678194 6.1630454 1
Produced by FastQC (version 0.10.1)