##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_B_12_GGCTAC_L002_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	35734689	
Filtered Sequences	0	
Sequence length	51	
%GC	45	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.704439235500274	33.0	31.0	34.0	30.0	34.0
2	31.802282986148278	34.0	31.0	34.0	30.0	34.0
3	31.842370560437786	34.0	31.0	34.0	30.0	34.0
4	35.23217742289572	37.0	35.0	37.0	33.0	37.0
5	35.26218568741427	37.0	35.0	37.0	33.0	37.0
6	35.296960384907784	37.0	35.0	37.0	33.0	37.0
7	35.33126777736893	37.0	35.0	37.0	33.0	37.0
8	35.291830999284755	37.0	35.0	37.0	33.0	37.0
9	36.8776259952899	39.0	37.0	39.0	34.0	39.0
10	36.87035132724955	39.0	38.0	39.0	34.0	39.0
11	36.8870581187932	39.0	38.0	39.0	34.0	39.0
12	36.83663652424679	39.0	37.0	39.0	34.0	39.0
13	36.82995371808049	39.0	38.0	39.0	33.0	39.0
14	38.24893735607997	40.0	38.0	41.0	34.0	41.0
15	38.16167450065117	40.0	38.0	41.0	33.0	41.0
16	38.1201784350215	40.0	38.0	41.0	33.0	41.0
17	38.048391214486294	40.0	38.0	41.0	33.0	41.0
18	38.066816588217684	40.0	38.0	41.0	33.0	41.0
19	38.038978903664166	40.0	38.0	41.0	33.0	41.0
20	38.017242433535664	40.0	38.0	41.0	33.0	41.0
21	38.023074777564176	40.0	38.0	41.0	33.0	41.0
22	38.043318916249696	40.0	38.0	41.0	33.0	41.0
23	38.03338291260909	40.0	38.0	41.0	33.0	41.0
24	37.92798647275201	40.0	38.0	41.0	33.0	41.0
25	37.80481374834408	40.0	38.0	41.0	33.0	41.0
26	37.7052431322405	40.0	38.0	41.0	32.0	41.0
27	37.59607772156629	40.0	38.0	41.0	32.0	41.0
28	37.533402151618	40.0	38.0	41.0	32.0	41.0
29	37.47459945712694	40.0	38.0	41.0	32.0	41.0
30	37.42662682750646	40.0	38.0	41.0	32.0	41.0
31	37.39564113738334	40.0	38.0	41.0	31.0	41.0
32	37.30514386175293	40.0	38.0	41.0	31.0	41.0
33	37.21235268061239	40.0	38.0	41.0	31.0	41.0
34	37.14210404909358	40.0	37.0	41.0	31.0	41.0
35	37.04906887534407	40.0	37.0	41.0	30.0	41.0
36	36.98222419117737	40.0	37.0	41.0	30.0	41.0
37	36.90965294255114	40.0	37.0	41.0	30.0	41.0
38	36.875999872280964	40.0	37.0	41.0	30.0	41.0
39	36.7910866385321	40.0	37.0	41.0	30.0	41.0
40	36.703774475272475	40.0	36.0	41.0	30.0	41.0
41	36.89251043992575	40.0	37.0	41.0	30.0	41.0
42	36.91010712308144	40.0	37.0	41.0	30.0	41.0
43	36.93456758501522	40.0	37.0	41.0	30.0	41.0
44	36.93415291791122	40.0	37.0	41.0	30.0	41.0
45	36.865794998243864	40.0	37.0	41.0	30.0	41.0
46	36.74862011531708	40.0	36.0	41.0	30.0	41.0
47	36.70323771391994	40.0	36.0	41.0	30.0	41.0
48	36.60515386044076	40.0	36.0	41.0	30.0	41.0
49	36.51883722844209	40.0	36.0	41.0	29.0	41.0
50	36.39052871007217	40.0	35.0	41.0	29.0	41.0
51	34.908850584931635	38.0	34.0	39.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	109.0
3	168.0
4	135.0
5	255.0
6	2475.0
7	49247.0
8	232296.0
9	281024.0
10	158061.0
11	80349.0
12	56077.0
13	53875.0
14	57255.0
15	60399.0
16	63514.0
17	65187.0
18	66751.0
19	69478.0
20	73791.0
21	79802.0
22	87265.0
23	98364.0
24	111658.0
25	128784.0
26	150115.0
27	177963.0
28	212187.0
29	257852.0
30	314978.0
31	396464.0
32	508908.0
33	678938.0
34	965306.0
35	1447637.0
36	2243504.0
37	3460832.0
38	7320375.0
39	1.5721188E7
40	2123.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	20.54369947537279	24.250645695458754	32.36081069327425	22.844844135894196
2	22.18636918634744	27.820301250765027	32.31779065047915	17.67553891240839
3	24.033306005572737	27.086842529831408	30.328594982298522	18.551256482297333
4	21.188114361697735	23.747924391557167	33.280723685751646	21.783237560993456
5	29.31123266405204	23.37287030269981	28.47184324195294	18.844053791295213
6	21.306083860126744	22.762070381916757	36.90573676330987	19.026108994646627
7	26.327404081545136	23.122662762744074	32.31921313330797	18.230720022402824
8	23.64928553178426	24.298311083947112	33.62036594720315	18.432037437065482
9	24.89680117938631	24.822515827475964	31.89494085521403	18.385742137923696
10	23.498562925280005	28.309885058088845	29.725112481256854	18.466439535374292
11	28.496546242973565	23.93812323271971	28.495286748766286	19.070043775540444
12	22.900145972170936	28.98521757824799	28.751801491058437	19.362834958522637
13	26.1738643699436	24.195444340402258	29.674985106612638	19.9557061830415
14	27.430401473311967	24.737039423829785	27.534845969068567	20.297713133789685
15	25.07295292821212	27.198216854842578	26.785872513551002	20.942957703394296
16	26.354162135386233	25.339046147763604	27.495591326568004	20.81120039028216
17	25.757558754381833	25.55950953735055	27.940737307047257	20.74219440122036
18	25.653137540458914	25.42455188937376	28.152512856840982	20.769797713326344
19	25.07769510195139	25.746622956855514	28.565376116407805	20.610305824785293
20	24.3904441005348	25.982819754344032	28.762686487802558	20.864049657318606
21	24.80670470023526	26.063375655838954	28.368619375975197	20.761300267950592
22	24.52267915533854	26.256617697957402	28.598165298987393	20.622537847716664
23	24.54236945876675	26.413546822753382	28.346683627973924	20.697400090505948
24	24.323615634413127	26.53834636514655	28.278723910336822	20.859314090103503
25	23.836534272668	27.177384876948828	28.16233722706044	20.82374362332273
26	23.212538533234813	27.74623070601211	28.128571900979825	20.912658859773256
27	23.57424773089518	27.420498192795463	28.001144826583836	21.00410924972552
28	23.32534294456078	27.705466460236316	27.957891589590172	21.01129900561273
29	24.058826683849208	26.99867112527868	27.81934472516824	21.123157465703873
30	24.128776723619996	26.874470350042117	27.819509456604543	21.17724346973334
31	24.33719727116853	26.710523473052938	27.69136169666394	21.26091755911459
32	24.32670392255879	26.77943899001356	27.656999528039794	21.236857559387857
33	23.949046115399756	27.054837831376155	27.642110464972937	21.354005588251155
34	24.225420986631434	26.92083827843339	27.5171809570728	21.336559777862373
35	23.872391949567497	27.27455997479741	27.460923359523896	21.392124716111194
36	23.444252828574612	27.341270706429487	27.778976407048418	21.435500057947483
37	23.218216801666188	27.094389312502777	28.01325334832362	21.674140537507416
38	22.88589330703478	26.88857672715075	28.693001223653237	21.53252874216124
39	22.808249877910907	26.722790002642054	28.343314730893574	22.125645388553462
40	23.320881724195054	26.731090647755586	27.914647626142457	22.033380001906906
41	24.150805926567838	26.629671801778954	27.61089922405111	21.608623047602094
42	24.111138969226285	26.528434307275372	27.677575493400514	21.682851230097828
43	24.3347429038212	26.56253179749335	27.49940299440818	21.60332230427727
44	24.481845291296523	26.500042387104	27.448430399521616	21.569681922077866
45	24.40768300740111	26.527800550213144	27.42262685414475	21.641889588240993
46	24.07153550704708	26.66273047489618	27.383247877299677	21.882486140757067
47	24.283257135937486	26.512601304243233	27.41625746361344	21.78788409620584
48	23.755506800663444	26.432214778631057	27.306543591908955	22.505734828796548
49	23.27172104712619	26.534428269789593	27.314443429225356	22.879407253858865
50	23.592084498632023	26.517788620532283	27.342543312459295	22.5475835683764
51	23.74131228127406	26.48760675910351	27.35139817210479	22.41968278751764
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	43.38854361126699
2	39.861908098755826
3	42.58456248787007
4	42.971351922691184
5	48.15528645534725
6	40.332192854773375
7	44.558124103947954
8	42.08132296884974
9	43.28254331731
10	41.9650024606543
11	47.566590018514006
12	42.26298093069357
13	46.1295705529851
14	47.72811460710165
15	46.015910631606424
16	47.16536252566839
17	46.4997531556022
18	46.422935253785255
19	45.688000926736684
20	45.25449375785341
21	45.56800496818585
22	45.14521700305521
23	45.2397695492727
24	45.182929724516626
25	44.66027789599073
26	44.12519739300807
27	44.578356980620704
28	44.33664195017351
29	45.18198414955308
30	45.30602019335333
31	45.59811483028312
32	45.56356148194665
33	45.30305170365091
34	45.56198076449381
35	45.26451666567869
36	44.8797528865221
37	44.89235733917361
38	44.41842204919602
39	44.93389526646436
40	45.35426172610196
41	45.75942897416993
42	45.793990199324114
43	45.93806520809847
44	46.051527213374385
45	46.049572595642104
46	45.95402164780415
47	46.071141232143326
48	46.26124162945999
49	46.15112830098505
50	46.13966806700842
51	46.16099506879169
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	52607.0
1	31392.0
2	10177.0
3	12353.5
4	14530.0
5	93286.0
6	172042.0
7	188793.5
8	205545.0
9	129075.5
10	52606.0
11	51953.5
12	51301.0
13	55631.0
14	59961.0
15	76818.5
16	93676.0
17	126107.5
18	158539.0
19	196380.5
20	234222.0
21	280968.5
22	327715.0
23	391545.5
24	455376.0
25	534220.0
26	710552.0
27	808040.0
28	897249.0
29	986458.0
30	1074686.5
31	1162915.0
32	1262158.0
33	1361401.0
34	1452994.0
35	1544587.0
36	1678464.0
37	1812341.0
38	1908752.5
39	2005164.0
40	2100398.5
41	2195633.0
42	2249722.0
43	2303811.0
44	2339610.0
45	2375409.0
46	2377770.5
47	2380132.0
48	2349994.5
49	2319857.0
50	2242334.0
51	2164811.0
52	2035840.5
53	1906870.0
54	1770455.5
55	1634041.0
56	1495805.5
57	1357570.0
58	1212145.5
59	1066721.0
60	953338.0
61	839955.0
62	724759.5
63	609564.0
64	528699.0
65	447834.0
66	383083.0
67	318332.0
68	295781.0
69	273230.0
70	253358.5
71	233487.0
72	208746.5
73	184006.0
74	165440.5
75	135924.5
76	124974.0
77	119833.5
78	114693.0
79	114688.0
80	114683.0
81	105823.5
82	96964.0
83	90510.0
84	84056.0
85	77573.5
86	71091.0
87	72290.0
88	73489.0
89	59391.5
90	45294.0
91	36919.5
92	28545.0
93	24038.5
94	19532.0
95	15994.5
96	12457.0
97	9399.0
98	6341.0
99	4253.0
100	2165.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0031482014576928318
2	0.006710566307153254
3	0.005020331924534169
4	0.010074244664617062
5	0.013068534051044911
6	0.0015866935346771872
7	0.029719021760620332
8	0.0031314110499184697
9	0.006176071659669404
10	8.143347770565457E-4
11	0.016961110253401114
12	0.01859817501140139
13	0.006682582294195984
14	0.015850144938997508
15	0.011515421331916447
16	0.03019754838218964
17	0.004622958940540941
18	0.001679040777436177
19	0.038189782482785845
20	0.005006339918055534
21	3.2461455030432753E-4
22	0.025426274172975175
23	6.156482850599315E-5
24	0.0446205086603664
25	0.016381841185185635
26	0.058872766459503824
27	0.07338527557914384
28	0.0025885211985474397
29	0.06722879272854453
30	0.016518962848676256
31	0.005274986442445322
32	0.0020820105640208596
33	0.006218047679105308
34	0.02543466937686235
35	0.014078756918802345
36	0.011361509260651463
37	0.01864015103083729
38	0.04086225572020509
39	0.024430043311696376
40	0.033197434571208946
41	0.022692236107049932
42	0.028011996970226886
43	0.029573504893242532
44	0.012542434607448243
45	0.036088183109694895
46	0.05113238847552304
47	0.02902221983798432
48	0.05846979667291914
49	0.01434740344319213
50	0.05684112711880604
51	0.11247334487785804
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.5734689E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	28.530690477303548
#Duplication Level	Relative count
1	100.0
2	11.318027210884354
3	3.6688906331763476
4	1.8642072213500784
5	1.2454212454212454
6	0.8869701726844584
7	0.6580324437467294
8	0.5422553636839351
9	0.43563579277864994
10++	10.202119309262166
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	86327	0.2415775886562214	No Hit
TTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	83891	0.23476068309983053	No Hit
TAACTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43661	0.12218099897273486	No Hit
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGGGG	13133545	9.378768	18.957727	43
TTTTT	24621550	7.489593	10.153844	18
AAAAA	9251810	4.452207	4.933497	47
TGTGT	10402190	4.4515986	38.244446	4
GTTTG	7808530	3.341647	37.610886	7
TGTTT	9191235	3.3162014	32.22524	6
TTTGA	8244430	3.2603848	34.95876	8
GTGTT	7573140	3.240912	37.700966	5
TTGTG	6939395	2.969702	37.788467	3
CTTTG	5810215	2.8918464	44.121418	1
GAGGG	4050585	2.672998	12.207174	9
TTTGT	6579330	2.373825	32.130455	2
TTGAG	5022685	2.3559623	23.676779	9
GGGGA	3541565	2.3370934	8.619806	22
AGGGG	3504825	2.3128486	10.760539	10
GGGAG	3407825	2.248838	5.294396	23
GGGGC	2528425	2.0999343	8.501048	45
TGAGG	3553460	1.9770063	13.445593	10
GGCCC	1681040	1.8885009	5.016051	46
ACTTT	4087920	1.8801928	22.253134	3
AACTT	3655280	1.8427303	24.994726	2
GGGCC	1876730	1.8127972	6.4240713	45
GGAGA	2885210	1.7594428	5.0336328	6
GGGAA	2874335	1.7528113	5.8199253	22
CGGGG	1933395	1.6057439	7.159655	40
TGAGC	2232785	1.4447585	5.6245155	10
TAACT	2835440	1.4294257	24.772512	1
GGCGG	1563345	1.2984059	7.3870535	13
GGGCG	1561655	1.2970023	6.4429255	12
TTGAT	3223950	1.2749598	8.64355	9
TGAGT	2580135	1.2102494	6.546221	10
GAGTG	2105760	1.1715626	5.039163	11
GCGGG	1375270	1.1422039	6.6181574	14
TTGAC	1984330	1.0825254	7.0659914	9
GTAAC	1284085	0.7678194	6.1630454	1
>>END_MODULE