FastQCFastQC Report
Mon 11 Aug 2014
140701_JD_B_12_GGCTAC_L002_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_B_12_GGCTAC_L002_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 35734689
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC 651909 1.824302990296068 TruSeq Adapter, Index 11 (100% over 51bp)
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 104853 0.2934207710608591 No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 44424 0.12431617916137455 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 19504675 6.290242 8.193788 18
AAAAA 10176965 4.325102 4.7362037 46
TGTGT 7469810 3.7194426 45.929306 4
CACAC 3447980 2.7149303 30.742428 12
TGTTT 6742925 2.7020712 36.964066 6
GTTTG 5290515 2.6343062 45.186687 7
CTTTG 4848455 2.5217018 47.34745 1
GTGTT 5047255 2.5131798 45.17905 5
TTTGA 5834290 2.4706225 38.664074 8
CTCCA 3177330 2.3674805 28.522581 24
TCCAG 3305265 2.3578074 27.428463 25
GGAAG 3356595 2.3191395 27.20655 5
TTTGT 5560560 2.228266 36.76229 2
TTGTG 4460135 2.220835 45.22489 3
GAAGA 3676585 2.1603422 23.268906 6
GAGGG 2646375 2.1499546 11.977611 11
GGGGG 2177835 2.0804288 5.065935 13
AAGAG 3495755 2.0540876 23.078548 7
AGAGC 2841150 2.050426 27.910269 8
GAGCA 2735100 1.9738909 27.807446 9
GCACA 2519075 1.8989478 28.78609 11
TTGAG 3599330 1.8939123 26.705853 9
TGAGG 2877205 1.8811736 15.985786 10
AGCAC 2446825 1.8444837 28.760405 10
CTGAA 3151905 1.8306412 22.467346 19
CCAGT 2557995 1.8247433 26.893082 26
TCTGA 3147470 1.7299032 21.238577 18
GTCTG 2670645 1.7259425 24.717758 17
CATCT 2961355 1.7000929 20.777195 39
CAGTC 2314905 1.6513351 26.680534 27
ATGCC 2214350 1.5796043 25.592587 47
ACTCC 2110020 1.5722103 27.807863 23
AGGGG 1924580 1.5635575 6.6229444 12
TGAAC 2661975 1.5460875 22.108229 20
AGTCA 2655970 1.5425998 21.800657 28
GTCAC 2152410 1.5354195 26.442186 29
GAACT 2593710 1.5064389 22.079704 21
AACTC 2430640 1.474595 22.906443 22
TGAGA 2543190 1.4141221 5.089182 10
TGAGC 2052555 1.4017639 6.047561 10
CTACA 2295515 1.3926189 22.334406 36
ACATC 2273885 1.3794965 21.512093 38
ATCTC 2296595 1.3184589 20.218483 40
GCTAC 1840275 1.3127583 26.199495 35
GGCTA 1895305 1.2943722 25.142632 34
CACGG 1443860 1.2798144 32.15089 31
CGGCT 1502845 1.2605677 30.658264 33
GAGTG 1872890 1.2245325 5.669888 11
ACGGC 1355580 1.2015644 32.10364 32
TGAGT 2260460 1.1894196 7.3204317 10
TACAT 2456375 1.1481665 16.910025 37
TTGAT 2663405 1.1278609 10.030157 9
GTATG 2005785 1.0554134 18.950033 45
CGGAA 1461430 1.0546976 27.114248 4
TGATT 2400455 1.0165106 6.0003414 10
ACACG 1332775 1.0046823 27.887108 13
TATGC 1781415 0.9790961 19.37145 46
CGTCT 1415285 0.9553799 24.959507 16
CTCGT 1415115 0.95526516 23.619797 42
CACGT 1328555 0.94772345 26.330215 14
TCTCG 1375660 0.92863125 23.362553 41
TTGAC 1685520 0.9263906 7.5737143 9
TCGGA 1340385 0.91539735 25.608784 3
TCACG 1245000 0.8881196 25.710129 30
GATCG 1270045 0.8673595 25.569933 1
ACGTC 1148215 0.81907797 26.225441 15
ATCGG 1167900 0.79760104 25.517838 2
TCGTA 1157455 0.63615704 19.249704 43
CGTAT 1149925 0.6320184 19.225384 44
Produced by FastQC (version 0.10.1)