##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_B_12_GGCTAC_L002_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	35734689	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.67113616128015	34.0	31.0	34.0	31.0	34.0
2	32.91322146388345	34.0	31.0	34.0	31.0	34.0
3	32.975185596270336	34.0	31.0	34.0	31.0	34.0
4	36.388392466491034	37.0	37.0	37.0	35.0	37.0
5	36.29934395119543	37.0	37.0	37.0	35.0	37.0
6	36.24825723262906	37.0	37.0	37.0	35.0	37.0
7	36.24135150581554	37.0	37.0	37.0	35.0	37.0
8	36.204526447676656	37.0	37.0	37.0	35.0	37.0
9	37.97533164483396	39.0	38.0	39.0	35.0	39.0
10	37.96223020158368	39.0	38.0	39.0	35.0	39.0
11	37.941967817321704	39.0	38.0	39.0	35.0	39.0
12	37.90216067082604	39.0	38.0	39.0	35.0	39.0
13	37.92210227434748	39.0	38.0	39.0	35.0	39.0
14	39.3791064755034	41.0	39.0	41.0	36.0	41.0
15	39.34448042908671	41.0	39.0	41.0	36.0	41.0
16	39.36707281823553	41.0	39.0	41.0	36.0	41.0
17	39.34604140531348	41.0	39.0	41.0	36.0	41.0
18	39.2234415416348	40.0	39.0	41.0	36.0	41.0
19	39.28684936925014	40.0	39.0	41.0	36.0	41.0
20	39.2470802810121	40.0	39.0	41.0	36.0	41.0
21	39.23373641225757	40.0	39.0	41.0	36.0	41.0
22	39.18347891596314	40.0	39.0	41.0	36.0	41.0
23	39.166207686878145	40.0	39.0	41.0	36.0	41.0
24	39.13106631486285	40.0	39.0	41.0	36.0	41.0
25	39.00915488588693	40.0	39.0	41.0	35.0	41.0
26	38.92462763562879	40.0	39.0	41.0	35.0	41.0
27	38.90186043035102	40.0	38.0	41.0	35.0	41.0
28	38.81879282620873	40.0	38.0	41.0	35.0	41.0
29	38.77870701491204	40.0	38.0	41.0	35.0	41.0
30	38.68244976191062	40.0	38.0	41.0	35.0	41.0
31	38.597733759485074	40.0	38.0	41.0	35.0	41.0
32	38.45984267555819	40.0	38.0	41.0	34.0	41.0
33	38.42424105048179	40.0	38.0	41.0	34.0	41.0
34	38.47728452317019	40.0	38.0	41.0	35.0	41.0
35	38.55370639996335	40.0	38.0	41.0	35.0	41.0
36	38.559988587000156	40.0	38.0	41.0	35.0	41.0
37	38.53018264689529	40.0	38.0	41.0	35.0	41.0
38	38.45462522424639	40.0	38.0	41.0	34.0	41.0
39	38.44049198245436	40.0	38.0	41.0	34.0	41.0
40	38.29742687840378	40.0	38.0	41.0	34.0	41.0
41	38.11823925485961	40.0	38.0	41.0	33.0	41.0
42	38.014266249805615	40.0	38.0	41.0	33.0	41.0
43	38.05630730408763	40.0	38.0	41.0	33.0	41.0
44	37.76805906999778	40.0	37.0	41.0	33.0	41.0
45	37.87906557686846	40.0	37.0	41.0	33.0	41.0
46	37.82637537435963	40.0	37.0	41.0	33.0	41.0
47	37.76539779036555	40.0	37.0	41.0	33.0	41.0
48	37.74957977107342	40.0	37.0	41.0	33.0	41.0
49	37.49599427044125	40.0	37.0	41.0	33.0	41.0
50	37.39092476780755	40.0	37.0	41.0	32.0	41.0
51	37.327296118345956	40.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	14.0
10	25.0
11	50.0
12	81.0
13	131.0
14	234.0
15	432.0
16	704.0
17	1190.0
18	2240.0
19	4131.0
20	7668.0
21	13712.0
22	22792.0
23	35688.0
24	53071.0
25	76095.0
26	104387.0
27	140109.0
28	183974.0
29	240358.0
30	309516.0
31	398740.0
32	514706.0
33	684473.0
34	969240.0
35	1463962.0
36	2224249.0
37	3548701.0
38	7398084.0
39	1.7327171E7
40	8756.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	21.102391826993728	26.322715400607017	28.14075751684865	24.4341352555506
2	21.179333690317097	27.997059431360405	31.204744647237405	19.618862231085096
3	19.740662639096882	24.94725060785036	35.0929653904829	20.21912136256985
4	21.247668268583435	24.93405592863445	31.82488457023221	21.99339123254991
5	28.32084532763109	24.95199272617148	27.492585705727002	19.23457624047043
6	23.26595874389728	24.51438992515088	32.0731348746312	20.146516456320633
7	26.104936858412287	27.08918776374408	27.285990931668664	19.519884446174977
8	21.82801423009446	27.242310685843663	31.155821728293198	19.773853355768676
9	22.817755598768468	26.000156318696376	31.364506908119445	19.817581174415704
10	21.40716545763138	27.327846619848856	31.3676187303603	19.897369192159474
11	28.209048317577732	25.030742827648357	26.773175944682553	19.987032910091354
12	21.357334325758366	30.13521679172862	26.258482339107523	22.248966543405484
13	23.82153519186974	27.743449954029213	27.669485883384827	20.765528970716215
14	23.03192564513434	25.957265222036774	27.975234372404923	23.03557476042397
15	23.30584995436787	28.03318086803554	27.667925135713368	20.993044041883223
16	23.1961746953452	25.910470567892435	27.588064081895624	23.305290654866738
17	25.167636391734465	26.171592263271172	27.452252884386464	21.208518460607902
18	22.944622915004217	26.083443626879976	29.8498034864934	21.12212997162241
19	22.472295757212272	26.323595540456502	28.108385104456907	23.095723597874322
20	22.180181839556514	26.388177045559285	30.2670606703755	21.164580444508697
21	24.3949905370661	26.39411805151012	27.956305986040626	21.254585425383162
22	22.15059714105809	28.67088895050969	28.00509051582903	21.173423392603194
23	22.22929658069782	28.683554514774144	27.848357656057953	21.23879124847008
24	22.035229134332905	26.645063561207433	27.83737700110203	23.48233030335764
25	22.100989881288736	26.764215018073894	29.760051920418277	21.374743180219085
26	22.107834770858087	26.73981016037386	27.622960423693627	23.529394645074426
27	22.135899937452933	26.712662869404014	27.55525030594222	23.596186887200837
28	22.13388632730993	28.886526975876407	27.55935430468441	21.42023239212925
29	24.346242386494534	26.775391832849028	27.436956286369245	21.44140949428719
30	22.17087435684693	26.722809872502317	29.600044931131205	21.506270839519548
31	22.274068203028154	26.683385995048113	27.35339602367884	23.689149778244886
32	22.31409933356353	28.837791200589436	27.2686800212533	21.57942944459374
33	22.290252622396654	26.679255372050648	27.232989198895257	23.797502806657445
34	24.37703101907836	26.759956282053736	27.187453231502396	21.67555946736551
35	24.44801029274672	26.708808549812357	27.16746385213087	21.67571730531006
36	22.39187956605246	26.67546802343787	27.15674890488619	23.77590350562348
37	22.35655108121971	26.6592301950634	29.31337670239693	21.670842021319956
38	22.31698168689813	28.75852368548667	27.277584534176302	21.646910093438898
39	22.260551924769796	26.63808267647159	27.21498429719089	23.88638110156772
40	22.28490226690849	28.776448128520926	27.224004654316538	21.71464495025405
41	22.376339193298243	26.653988853035486	29.251768969753055	21.717902983913213
42	22.225236772478407	26.599513058873132	27.2371590000295	23.938091168618968
43	22.27658249631387	26.71272991666096	29.205073968438406	21.805613618586765
44	22.483312660371237	26.622808868437687	27.08915384209827	23.804724629092807
45	24.495700887230623	26.512961702777137	27.083650666489877	21.907686743502357
46	22.284835552321717	26.691801053640518	29.204278927889295	21.819084466148475
47	22.289298748824233	28.806548417818316	27.11623953773635	21.787913295621095
48	22.269841792692446	26.61335522367726	29.117312386381734	21.99949059724856
49	24.47758535130948	26.608148177811202	27.03109295284478	21.883173518034535
50	22.316124906738487	26.449142629427612	27.006549584197163	24.228182879636734
51	22.35238546142711	26.568687637795524	26.98135736626134	24.097569534516026
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	45.53652708254433
2	40.79819592140219
3	39.95978400166674
4	43.24105950113334
5	47.555421568101515
6	43.41247520021791
7	45.62482130458726
8	41.60186758586314
9	42.63533677318417
10	41.30453464979085
11	48.19608122766908
12	43.60630086916385
13	44.58706416258595
14	46.067500405558306
15	44.298893996251095
16	46.50146535021194
17	46.37615485234237
18	44.06675288662663
19	45.56801935508659
20	43.34476228406521
21	45.64957596244926
22	43.32402053366128
23	43.4680878291679
24	45.517559437690544
25	43.475733061507825
26	45.63722941593251
27	45.73208682465377
28	43.55411871943918
29	45.78765188078172
30	43.67714519636648
31	45.96321798127304
32	43.89352877815727
33	46.08775542905409
34	46.05259048644387
35	46.12372759805678
36	46.16778307167594
37	44.02739310253966
38	43.96389178033703
39	46.146933026337514
40	43.99954721716254
41	44.09424217721145
42	46.16332794109737
43	44.08219611490063
44	46.28803728946404
45	46.403387630732986
46	44.10392001847019
47	44.07721204444533
48	44.269332389941006
49	46.36075886934402
50	46.54430778637522
51	46.44995499594314
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	82755.0
1	48636.5
2	14518.0
3	15853.5
4	17189.0
5	47808.5
6	78428.0
7	125068.0
8	171708.0
9	106023.0
10	40338.0
11	44001.5
12	47665.0
13	53425.5
14	59186.0
15	78457.0
16	97728.0
17	135338.0
18	172948.0
19	213605.5
20	254263.0
21	300575.0
22	346887.0
23	415141.0
24	483395.0
25	566952.0
26	750749.0
27	850989.0
28	929188.5
29	1007388.0
30	1087666.5
31	1167945.0
32	1262792.0
33	1357639.0
34	1443197.5
35	1528756.0
36	1655920.0
37	1783084.0
38	1871947.5
39	1960811.0
40	2047065.5
41	2133320.0
42	2183414.5
43	2233509.0
44	2271494.0
45	2309479.0
46	2311256.5
47	2313034.0
48	2300319.0
49	2287604.0
50	2219926.5
51	2152249.0
52	2056950.0
53	1961651.0
54	2155407.5
55	2349164.0
56	1883204.5
57	1417245.0
58	1257285.5
59	1097326.0
60	978591.5
61	859857.0
62	737488.5
63	615120.0
64	530346.0
65	445572.0
66	374978.5
67	304385.0
68	281412.0
69	258439.0
70	234010.0
71	209581.0
72	179307.0
73	149033.0
74	124414.0
75	83484.0
76	67173.0
77	63108.5
78	59044.0
79	60795.5
80	62547.0
81	54799.0
82	47051.0
83	42385.5
84	37720.0
85	34828.5
86	31937.0
87	37429.5
88	42922.0
89	27735.0
90	12548.0
91	7360.0
92	2172.0
93	1520.5
94	869.0
95	518.5
96	168.0
97	102.0
98	36.0
99	23.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025966365623050477
2	2.9103373475560396E-4
3	4.309537995419521E-4
4	0.03144003855749242
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0032209598913817326
12	0.0
13	0.0031426046551013775
14	0.0
15	0.0
16	6.380354954257472E-4
17	0.007667619550291874
18	0.0014103942530463887
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0017601944150122588
25	0.0
26	0.0
27	0.0
28	5.596802591453923E-6
29	0.0
30	0.0
31	0.0
32	0.0
33	3.0782414252996575E-5
34	4.617362137949487E-4
35	2.7984012957269618E-5
36	1.315248608991672E-4
37	0.0
38	0.0
39	0.0
40	3.3300975419150845E-4
41	0.006856083174531056
42	0.001665048770957542
43	1.5671047256070984E-4
44	0.0011557397351352353
45	0.0019253000914601494
46	0.004026899464551098
47	0.007552885097167069
48	0.007410166631084994
49	0.0
50	0.016387437987777087
51	0.06255266416338477
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.5734689E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	31.689003420835338
#Duplication Level	Relative count
1	100.0
2	12.226450683084607
3	3.9152005419514238
4	2.044936805541719
5	1.3309689374165654
6	0.9351318682048523
7	0.7358850212862048
8	0.5625402644669815
9	0.45162618634893437
10++	10.628490970797055
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC	651909	1.824302990296068	TruSeq Adapter, Index 11 (100% over 51bp)
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	104853	0.2934207710608591	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	44424	0.12431617916137455	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	19504675	6.290242	8.193788	18
AAAAA	10176965	4.325102	4.7362037	46
TGTGT	7469810	3.7194426	45.929306	4
CACAC	3447980	2.7149303	30.742428	12
TGTTT	6742925	2.7020712	36.964066	6
GTTTG	5290515	2.6343062	45.186687	7
CTTTG	4848455	2.5217018	47.34745	1
GTGTT	5047255	2.5131798	45.17905	5
TTTGA	5834290	2.4706225	38.664074	8
CTCCA	3177330	2.3674805	28.522581	24
TCCAG	3305265	2.3578074	27.428463	25
GGAAG	3356595	2.3191395	27.20655	5
TTTGT	5560560	2.228266	36.76229	2
TTGTG	4460135	2.220835	45.22489	3
GAAGA	3676585	2.1603422	23.268906	6
GAGGG	2646375	2.1499546	11.977611	11
GGGGG	2177835	2.0804288	5.065935	13
AAGAG	3495755	2.0540876	23.078548	7
AGAGC	2841150	2.050426	27.910269	8
GAGCA	2735100	1.9738909	27.807446	9
GCACA	2519075	1.8989478	28.78609	11
TTGAG	3599330	1.8939123	26.705853	9
TGAGG	2877205	1.8811736	15.985786	10
AGCAC	2446825	1.8444837	28.760405	10
CTGAA	3151905	1.8306412	22.467346	19
CCAGT	2557995	1.8247433	26.893082	26
TCTGA	3147470	1.7299032	21.238577	18
GTCTG	2670645	1.7259425	24.717758	17
CATCT	2961355	1.7000929	20.777195	39
CAGTC	2314905	1.6513351	26.680534	27
ATGCC	2214350	1.5796043	25.592587	47
ACTCC	2110020	1.5722103	27.807863	23
AGGGG	1924580	1.5635575	6.6229444	12
TGAAC	2661975	1.5460875	22.108229	20
AGTCA	2655970	1.5425998	21.800657	28
GTCAC	2152410	1.5354195	26.442186	29
GAACT	2593710	1.5064389	22.079704	21
AACTC	2430640	1.474595	22.906443	22
TGAGA	2543190	1.4141221	5.089182	10
TGAGC	2052555	1.4017639	6.047561	10
CTACA	2295515	1.3926189	22.334406	36
ACATC	2273885	1.3794965	21.512093	38
ATCTC	2296595	1.3184589	20.218483	40
GCTAC	1840275	1.3127583	26.199495	35
GGCTA	1895305	1.2943722	25.142632	34
CACGG	1443860	1.2798144	32.15089	31
CGGCT	1502845	1.2605677	30.658264	33
GAGTG	1872890	1.2245325	5.669888	11
ACGGC	1355580	1.2015644	32.10364	32
TGAGT	2260460	1.1894196	7.3204317	10
TACAT	2456375	1.1481665	16.910025	37
TTGAT	2663405	1.1278609	10.030157	9
GTATG	2005785	1.0554134	18.950033	45
CGGAA	1461430	1.0546976	27.114248	4
TGATT	2400455	1.0165106	6.0003414	10
ACACG	1332775	1.0046823	27.887108	13
TATGC	1781415	0.9790961	19.37145	46
CGTCT	1415285	0.9553799	24.959507	16
CTCGT	1415115	0.95526516	23.619797	42
CACGT	1328555	0.94772345	26.330215	14
TCTCG	1375660	0.92863125	23.362553	41
TTGAC	1685520	0.9263906	7.5737143	9
TCGGA	1340385	0.91539735	25.608784	3
TCACG	1245000	0.8881196	25.710129	30
GATCG	1270045	0.8673595	25.569933	1
ACGTC	1148215	0.81907797	26.225441	15
ATCGG	1167900	0.79760104	25.517838	2
TCGTA	1157455	0.63615704	19.249704	43
CGTAT	1149925	0.6320184	19.225384	44
>>END_MODULE