FastQCFastQC Report
Mon 11 Aug 2014
140701_JD_B_10_GATCAG_L002_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_B_10_GATCAG_L002_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 40487517
Filtered Sequences 0
Sequence length 51
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 90272 0.22296254917287223 No Hit
TTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 67267 0.16614256685585335 No Hit
TAACTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 40892 0.10099903138046228 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 25183865 6.3973594 8.454038 18
TGTGT 9984815 4.103337 36.116318 4
AAAAA 10906950 4.0210395 4.36048 47
GTTTG 7347315 3.0194356 35.434692 7
TGTTT 9080725 2.9339874 28.458801 6
GTGTT 7031260 2.8895502 35.479515 5
TTTGA 7902635 2.7508125 30.215921 8
TTGTG 6550420 2.6919456 35.546932 3
CTTTG 5984050 2.6572917 38.63202 1
GGGGG 3078500 2.6032412 5.440719 13
GAGGG 3305525 2.3675964 11.096359 11
TTTGT 6903815 2.2306268 28.295107 2
TTGAG 4935240 2.1850266 22.329733 9
TGAGG 3666480 2.0647008 13.550295 10
AGGGG 2461645 1.7631638 6.2981153 12
AACTT 3938170 1.5958122 17.818832 2
ACTTT 4233670 1.5924028 16.135633 3
TGAGC 2471685 1.5040001 5.3912544 10
GAGTG 2248320 1.266094 5.2495494 11
TGAGT 2808960 1.2436379 6.447065 10
TAACT 2966865 1.2022231 17.599865 1
TTGAT 3345505 1.16453 7.6321573 9
TTGAC 2074360 0.99238336 5.9080973 9
GTAAC 1413705 0.7286274 5.0086403 1
Produced by FastQC (version 0.10.1)