##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_B_10_GATCAG_L002_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	40487517	
Filtered Sequences	0	
Sequence length	51	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14950556241816	33.0	31.0	34.0	30.0	34.0
2	32.32270847826998	34.0	31.0	34.0	31.0	34.0
3	32.382641197779556	34.0	31.0	34.0	31.0	34.0
4	35.84334581446424	37.0	35.0	37.0	35.0	37.0
5	35.849185589721394	37.0	35.0	37.0	35.0	37.0
6	35.849401582221006	37.0	36.0	37.0	35.0	37.0
7	35.88706810546075	37.0	36.0	37.0	35.0	37.0
8	35.885872057799936	37.0	36.0	37.0	35.0	37.0
9	37.55796479196292	39.0	38.0	39.0	35.0	39.0
10	37.55324360839416	39.0	38.0	39.0	35.0	39.0
11	37.56510674635839	39.0	38.0	39.0	35.0	39.0
12	37.530551009092505	39.0	38.0	39.0	35.0	39.0
13	37.482282699628136	39.0	38.0	39.0	35.0	39.0
14	38.91845140812167	41.0	39.0	41.0	36.0	41.0
15	38.87669658774086	41.0	39.0	41.0	36.0	41.0
16	38.83011203181464	41.0	39.0	41.0	36.0	41.0
17	38.77654762083829	41.0	39.0	41.0	35.0	41.0
18	38.77396309583519	40.0	39.0	41.0	35.0	41.0
19	38.774721156646876	40.0	39.0	41.0	35.0	41.0
20	38.761908491449354	40.0	39.0	41.0	35.0	41.0
21	38.7329432180294	40.0	39.0	41.0	35.0	41.0
22	38.6714838551349	40.0	39.0	41.0	35.0	41.0
23	38.585775215605345	40.0	39.0	41.0	34.0	41.0
24	38.52287254365339	40.0	38.0	41.0	34.0	41.0
25	38.504400652675244	40.0	38.0	41.0	34.0	41.0
26	38.44705675578969	40.0	38.0	41.0	34.0	41.0
27	38.377870665667146	40.0	38.0	41.0	34.0	41.0
28	38.32413788180688	40.0	38.0	41.0	34.0	41.0
29	38.25980965935747	40.0	38.0	41.0	34.0	41.0
30	38.21477175298253	40.0	38.0	41.0	34.0	41.0
31	38.15510133654281	40.0	38.0	41.0	34.0	41.0
32	38.08379567954241	40.0	38.0	41.0	33.0	41.0
33	38.02057088361334	40.0	38.0	41.0	33.0	41.0
34	37.95196393495803	40.0	38.0	41.0	33.0	41.0
35	37.8738062894793	40.0	38.0	41.0	33.0	41.0
36	37.8129258457613	40.0	38.0	41.0	33.0	41.0
37	37.721765945785215	40.0	38.0	41.0	33.0	41.0
38	37.67470907144046	40.0	38.0	41.0	32.0	41.0
39	37.594819929313026	40.0	37.0	41.0	32.0	41.0
40	37.51823014980148	40.0	37.0	41.0	32.0	41.0
41	37.69404923003799	40.0	38.0	41.0	33.0	41.0
42	37.73077104234374	40.0	38.0	41.0	33.0	41.0
43	37.73157983484144	40.0	38.0	41.0	33.0	41.0
44	37.684397218036366	40.0	38.0	41.0	33.0	41.0
45	37.640916087790714	40.0	37.0	41.0	33.0	41.0
46	37.564249667372785	40.0	37.0	41.0	32.0	41.0
47	37.520795582500156	40.0	37.0	41.0	32.0	41.0
48	37.45084851708738	40.0	37.0	41.0	32.0	41.0
49	37.373657021249294	40.0	37.0	41.0	32.0	41.0
50	37.26503588748107	40.0	37.0	41.0	31.0	41.0
51	35.75998868984729	38.0	35.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	120.0
3	174.0
4	145.0
5	197.0
6	668.0
7	2836.0
8	9463.0
9	14697.0
10	18792.0
11	24511.0
12	34129.0
13	46898.0
14	57367.0
15	63982.0
16	68352.0
17	71201.0
18	73613.0
19	76818.0
20	80809.0
21	87068.0
22	95530.0
23	106575.0
24	121473.0
25	139410.0
26	163043.0
27	192069.0
28	230033.0
29	278799.0
30	342626.0
31	431844.0
32	555133.0
33	743926.0
34	1055466.0
35	1582464.0
36	2452803.0
37	3853377.0
38	8324409.0
39	1.9083805E7
40	2892.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.203287339105827	26.120148475136247	31.42441481352677	23.252149372231155
2	21.466959171255112	28.513150664934166	31.67038225547912	18.3495079083316
3	22.857169109937324	27.45570597818685	30.314698457615158	19.37242645426067
4	21.406372414965826	24.946141583405705	32.09480938869378	21.55267661293468
5	27.19746742825298	24.89772163893388	28.65209547808331	19.252715454729824
6	20.69052033116202	24.520310760355038	34.96243191221731	19.82673699626563
7	24.95653725684872	24.81509109554278	31.08602244554486	19.14234920206364
8	22.8050199849297	25.01545894944055	32.79245596845369	19.387065097176063
9	22.92383480701946	25.485962216761376	32.208725559984714	19.381477416234446
10	22.74427428802669	27.373083813765103	30.475371075376135	19.40727082283207
11	26.192747293700215	25.0808059091752	28.959158527158696	19.76728826996588
12	21.508439211268314	29.470546054724718	29.123434132096797	19.89758060191017
13	24.03220194224677	25.58380297298517	29.999180185394685	20.384814899373378
14	24.963835437859412	26.01521452312592	28.387645371856234	20.633304667158438
15	23.655282327023652	27.87766443640628	27.740388571251284	20.726664665318783
16	24.238271532923466	26.466395473556076	28.290042274510846	21.005290719009608
17	23.848440755748086	26.60173527841635	28.526421747857206	21.023402217978354
18	23.636612741177736	26.496757917909576	28.732166004463032	21.134463336449656
19	23.263045161448456	26.760094370746057	28.97344683704215	21.003413630763337
20	22.90379641331128	26.80325655551141	29.073692995483118	21.21925403569418
21	22.981784425850947	26.950842740234137	28.90522917668162	21.1621436572333
22	22.648908563932572	27.150855362554278	29.121795957242323	21.078440116270826
23	22.627422106784618	27.266384571230418	28.950774412238054	21.155418909746913
24	22.463817231512614	27.360014169693496	28.87974246068104	21.29642613811285
25	22.488791706303612	27.47326329530566	28.786381825273367	21.251563173117358
26	22.461880450983124	27.44967358016584	28.768001294592853	21.320444674258184
27	22.48543052142442	27.43486860119609	28.65688363217105	21.422817245208446
28	22.444720574951273	27.504778630857917	28.62958094764051	21.4209198465503
29	22.53013035323759	27.467209023529026	28.526804388829635	21.475856234403746
30	22.5174637915659	27.446504400840894	28.466452079869764	21.569579727723447
31	22.61010648666716	27.416288184958525	28.37872051001837	21.59488481835595
32	22.614608483022224	27.485414363958576	28.29135210902332	21.608625043995875
33	22.59641548156907	27.424189684908423	28.249181820265644	21.730213013256865
34	22.642355798964978	27.46427458456495	28.206578784118648	21.68679083235142
35	22.67626329281851	27.421572207134343	28.14681547553542	21.755349024511723
36	22.648839144598877	27.41892889275433	28.16741191458162	21.764820048065175
37	22.685975965172393	27.39028051024212	28.14926817523814	21.774475349347345
38	22.60529195898492	27.437974960411655	28.216841282371153	21.739891798232275
39	22.64442667768972	27.3285830844014	28.174349673614525	21.852640564294354
40	22.63288643263255	27.383629009868276	28.178020682339668	21.80546387515951
41	22.651529431586603	27.38250377796789	28.152253156088562	21.81371363435694
42	22.547553795131535	27.346246515258986	28.166162323861826	21.94003736574765
43	22.597434324211303	27.40574291289592	28.102195263307717	21.894627499585056
44	22.62233171092913	27.372110305568548	28.08189197117911	21.92366601232321
45	22.64574017068853	27.373582634958638	28.022261117383206	21.958416076969627
46	22.527917988945983	27.499697282261927	27.96051028573095	22.011874443061142
47	22.612761279253256	27.384403686883097	28.009151266956252	21.993683766907395
48	22.545572208247748	27.347133285180075	27.954134184080953	22.15316032249122
49	22.610624831436322	27.412758554265487	27.935038553437458	22.041578060860733
50	22.573318875244517	27.40228693039087	27.98914140788828	22.035252786476338
51	22.601446214985764	27.348400627860258	27.95238231823653	22.097770838917448
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	42.455436711336986
2	39.816467079586715
3	42.229595564198
4	42.959049027900505
5	46.4501828829828
6	40.51725732742765
7	44.09888645891236
8	42.19208508210576
9	42.30531222325391
10	42.15154511085876
11	45.9600355636661
12	41.40601981317849
13	44.417016841620146
14	45.59714010501785
15	44.381946992342435
16	45.24356225193308
17	44.87184297372644
18	44.77107607762739
19	44.26645879221179
20	44.12305044900546
21	44.14392808308424
22	43.7273486802034
23	43.782841016531535
24	43.760243369625464
25	43.74035487942097
26	43.78232512524131
27	43.90824776663286
28	43.865640421501574
29	44.00598658764134
30	44.087043519289345
31	44.204991305023114
32	44.223233527018095
33	44.32662849482594
34	44.329146631316405
35	44.431612317330234
36	44.41365919266405
37	44.46045131451974
38	44.3451837572172
39	44.49706724198407
40	44.43835030779206
41	44.46524306594355
42	44.487591160879184
43	44.492061823796355
44	44.54599772325234
45	44.60415624765815
46	44.539792432007125
47	44.60644504616065
48	44.69873253073897
49	44.652202892297055
50	44.60857166172086
51	44.699217053903205
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	52688.0
1	31851.0
2	11014.0
3	14163.5
4	17313.0
5	82767.0
6	148221.0
7	177545.5
8	206870.0
9	130758.0
10	54646.0
11	54709.0
12	54772.0
13	61871.5
14	68971.0
15	90496.0
16	112021.0
17	154357.0
18	196693.0
19	244310.5
20	291928.0
21	345839.5
22	399751.0
23	479135.5
24	558520.0
25	658583.5
26	880217.5
27	1001788.0
28	1105768.0
29	1209748.0
30	1304484.5
31	1399221.0
32	1518307.5
33	1637394.0
34	1744814.0
35	1852234.0
36	2010818.0
37	2169402.0
38	2275345.5
39	2381289.0
40	2484397.0
41	2587505.0
42	2639911.0
43	2692317.0
44	2727323.0
45	2762329.0
46	2748053.5
47	2733778.0
48	2686250.0
49	2638722.0
50	2544280.5
51	2449839.0
52	2302901.5
53	2155964.0
54	1998982.5
55	1842001.0
56	1678975.5
57	1515950.0
58	1355681.0
59	1195412.0
60	1057945.0
61	920478.0
62	790961.5
63	661445.0
64	565830.0
65	470215.0
66	394279.5
67	318344.0
68	283442.0
69	248540.0
70	218032.5
71	187525.0
72	158921.0
73	130317.0
74	108790.0
75	74069.0
76	60875.0
77	55475.0
78	50075.0
79	50015.5
80	49956.0
81	44018.0
82	38080.0
83	34180.5
84	30281.0
85	27110.0
86	23939.0
87	27753.5
88	31568.0
89	22193.5
90	12819.0
91	8635.0
92	4451.0
93	3343.5
94	2236.0
95	1782.5
96	1329.0
97	989.0
98	649.0
99	416.5
100	184.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.003154058570694765
2	0.006624263967582897
3	0.005016360968740068
4	0.010022842349161594
5	0.013206539684811988
6	0.0015510953660112076
7	0.029752380221291414
8	0.0031120703203409584
9	0.006243899817319002
10	8.027165508815964E-4
11	0.016983012319574945
12	0.01849952912647125
13	0.006802096557316666
14	0.01627662175479914
15	0.01185550598225127
16	0.03037973407951888
17	0.004717503422104152
18	0.0017931452798155044
19	0.03866129898753732
20	0.005041059939536426
21	3.2108662035263856E-4
22	0.025427590434849337
23	9.138619194652021E-5
24	0.044571762699105504
25	0.016192645254091524
26	0.05885764740771828
27	0.07408456290367227
28	0.002531644506626573
29	0.06686505373989717
30	0.016343308975949305
31	0.0051200966460847675
32	0.0018944110600805674
33	0.006236490126080095
34	0.02546710878812351
35	0.014300704091090594
36	0.011282489859775792
37	0.01895892998328349
38	0.04110896699345628
39	0.02449890913290632
40	0.032790353629243305
41	0.023234321828132853
42	0.028181525678643123
43	0.029883284766512107
44	0.012865693887822265
45	0.036100015715955114
46	0.05131705162359056
47	0.029169484510497397
48	0.05842788531586168
49	0.014283414811533144
50	0.05673600581631124
51	0.11212591772422102
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4.0487517E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	32.59602711353794
#Duplication Level	Relative count
1	100.0
2	14.657839419524697
3	5.330567835019869
4	2.752927866585657
5	1.7633543539139482
6	1.2228251381003261
7	0.9609408281131061
8	0.7591153198829552
9	0.611761748130146
10++	11.61229948391332
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	90272	0.22296254917287223	No Hit
TTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	67267	0.16614256685585335	No Hit
TAACTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40892	0.10099903138046228	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	25183865	6.3973594	8.454038	18
TGTGT	9984815	4.103337	36.116318	4
AAAAA	10906950	4.0210395	4.36048	47
GTTTG	7347315	3.0194356	35.434692	7
TGTTT	9080725	2.9339874	28.458801	6
GTGTT	7031260	2.8895502	35.479515	5
TTTGA	7902635	2.7508125	30.215921	8
TTGTG	6550420	2.6919456	35.546932	3
CTTTG	5984050	2.6572917	38.63202	1
GGGGG	3078500	2.6032412	5.440719	13
GAGGG	3305525	2.3675964	11.096359	11
TTTGT	6903815	2.2306268	28.295107	2
TTGAG	4935240	2.1850266	22.329733	9
TGAGG	3666480	2.0647008	13.550295	10
AGGGG	2461645	1.7631638	6.2981153	12
AACTT	3938170	1.5958122	17.818832	2
ACTTT	4233670	1.5924028	16.135633	3
TGAGC	2471685	1.5040001	5.3912544	10
GAGTG	2248320	1.266094	5.2495494	11
TGAGT	2808960	1.2436379	6.447065	10
TAACT	2966865	1.2022231	17.599865	1
TTGAT	3345505	1.16453	7.6321573	9
TTGAC	2074360	0.99238336	5.9080973	9
GTAAC	1413705	0.7286274	5.0086403	1
>>END_MODULE