FastQCFastQC Report
Mon 11 Aug 2014
140701_JD_B_10_GATCAG_L002_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_B_10_GATCAG_L002_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 40487517
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 118267 0.2921073179172731 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 22224850 5.9540434 7.656367 18
AAAAA 11158730 3.8994997 4.1863093 45
TGTGT 7732875 3.3885794 39.950283 4
TGTTT 7240215 2.4807122 31.344193 6
GTTTG 5478360 2.400641 39.26574 7
CTTTG 5186260 2.3724613 41.164158 1
GTGTT 5130845 2.2483582 39.237892 5
TTTGA 6090240 2.200612 32.613586 8
GAGGG 2841580 2.1479502 11.3781 11
TTTGT 6069095 2.0794516 31.138437 2
TTGTG 4705610 2.0620184 39.295086 3
TGAGG 3162485 1.8691366 14.369916 10
TTGAG 3823120 1.7667648 23.438133 9
AGGGG 2132515 1.6119679 6.392594 12
TGAGC 2331380 1.438447 5.530727 10
GAGTG 2070940 1.2239962 5.3953853 11
TGAGT 2550875 1.1788268 6.7447796 10
TTGAT 2958560 1.069029 8.845723 9
TGATT 2769520 1.0007223 5.638653 10
TTGAC 1867855 0.90109843 6.260845 9
Produced by FastQC (version 0.10.1)