##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_B_10_GATCAG_L002_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	40487517	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.72193953015197	34.0	31.0	34.0	31.0	34.0
2	32.95923397821605	34.0	31.0	34.0	31.0	34.0
3	33.023106603450145	34.0	31.0	34.0	31.0	34.0
4	36.41820314641671	37.0	37.0	37.0	35.0	37.0
5	36.33600314388259	37.0	37.0	37.0	35.0	37.0
6	36.290727015934316	37.0	37.0	37.0	35.0	37.0
7	36.28558278839377	37.0	37.0	37.0	35.0	37.0
8	36.242752105544035	37.0	37.0	37.0	35.0	37.0
9	38.024912542796834	39.0	38.0	39.0	35.0	39.0
10	38.02079164301431	39.0	38.0	39.0	35.0	39.0
11	37.99351590269168	39.0	38.0	39.0	35.0	39.0
12	37.95296392218866	39.0	38.0	39.0	35.0	39.0
13	37.971207150095175	39.0	38.0	39.0	35.0	39.0
14	39.424725872915346	41.0	39.0	41.0	37.0	41.0
15	39.390637193187224	41.0	39.0	41.0	36.0	41.0
16	39.41025704293005	41.0	39.0	41.0	36.0	41.0
17	39.400827568655295	41.0	39.0	41.0	36.0	41.0
18	39.32205956221025	41.0	39.0	41.0	36.0	41.0
19	39.3599550202103	41.0	39.0	41.0	36.0	41.0
20	39.31620686939137	41.0	39.0	41.0	36.0	41.0
21	39.305553314123955	40.0	39.0	41.0	36.0	41.0
22	39.25729359990142	40.0	39.0	41.0	36.0	41.0
23	39.232451720860034	40.0	39.0	41.0	36.0	41.0
24	39.19431505271118	40.0	39.0	41.0	36.0	41.0
25	39.08757037385128	40.0	39.0	41.0	36.0	41.0
26	39.00980531851336	40.0	39.0	41.0	35.0	41.0
27	38.97939574807712	40.0	39.0	41.0	35.0	41.0
28	38.924504137905025	40.0	38.0	41.0	35.0	41.0
29	38.877603311657765	40.0	38.0	41.0	35.0	41.0
30	38.80572004452632	40.0	38.0	41.0	35.0	41.0
31	38.715462397953424	40.0	38.0	41.0	35.0	41.0
32	38.62416895064224	40.0	38.0	41.0	35.0	41.0
33	38.54406594012668	40.0	38.0	41.0	35.0	41.0
34	38.58556594122579	40.0	38.0	41.0	35.0	41.0
35	38.675059500438124	40.0	38.0	41.0	35.0	41.0
36	38.67019735984304	40.0	38.0	41.0	35.0	41.0
37	38.65111528079136	40.0	38.0	41.0	35.0	41.0
38	38.61744470524088	40.0	38.0	41.0	35.0	41.0
39	38.56898846130772	40.0	38.0	41.0	35.0	41.0
40	38.49801204158803	40.0	38.0	41.0	35.0	41.0
41	38.416363863459445	40.0	38.0	41.0	34.0	41.0
42	38.25211575706162	40.0	38.0	41.0	34.0	41.0
43	38.28134627272895	40.0	38.0	41.0	34.0	41.0
44	37.94390572284292	40.0	38.0	41.0	33.0	41.0
45	38.072789052487465	40.0	38.0	41.0	33.0	41.0
46	38.08303314821702	40.0	38.0	41.0	33.0	41.0
47	38.02625754995052	40.0	38.0	41.0	33.0	41.0
48	37.972085470195665	40.0	37.0	41.0	33.0	41.0
49	37.73115750713979	40.0	37.0	41.0	33.0	41.0
50	37.61945764666181	40.0	37.0	41.0	33.0	41.0
51	37.532243851851916	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	15.0
10	23.0
11	38.0
12	86.0
13	167.0
14	256.0
15	455.0
16	754.0
17	1214.0
18	2198.0
19	4116.0
20	7576.0
21	13234.0
22	21649.0
23	33974.0
24	50777.0
25	72973.0
26	99121.0
27	136260.0
28	182703.0
29	240995.0
30	315204.0
31	411663.0
32	537138.0
33	721012.0
34	1027027.0
35	1551864.0
36	2386996.0
37	3870964.0
38	8354527.0
39	2.0432033E7
40	10503.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.18862569339289	27.434245952496738	28.898567020147613	24.47856133396276
2	21.215500341056785	27.162084750677824	31.718345016682107	19.90406989158329
3	20.189937006941285	26.20685321759273	32.92659938601021	20.676610389455778
4	21.2757049753427	26.07210906637181	32.230286258533425	20.421899699752068
5	25.96011753449835	26.137601868743886	28.17992024554136	19.722360351216402
6	21.649608692970727	25.82416451964688	31.92753460282585	20.598692184556537
7	25.872411489200488	26.269971062932807	27.83455700679298	20.023060441073724
8	21.854718826051993	26.330624325517416	31.44160211158417	20.37305473684642
9	20.966022687931197	26.78782697392878	31.85217557302909	20.393974765110936
10	21.564510858988957	26.362145152047727	31.67643498612177	20.396909002841543
11	25.643628233380905	26.220885801751017	27.66941673545544	20.466069229412646
12	21.49343710062536	30.6623273538854	27.2892432499627	20.554992295526546
13	23.69053949814666	26.66841375842167	28.58858533238256	21.052461411049112
14	23.033309254306705	26.967541625237228	28.827127136494934	21.17202198396113
15	23.252744790449857	26.96625233896166	28.493634223111286	21.287368647477198
16	23.137021432021722	26.91670292707914	28.483945588658706	21.462330052240432
17	23.05095568902554	27.084617157753282	28.383368275094266	21.481058878126913
18	22.949103876256196	27.051569181370503	28.56987851001096	21.42944843236234
19	22.54888834007776	27.255889759799295	28.89429104778147	21.300930852341477
20	22.29753926376863	27.295847754753645	28.95323761148405	21.45337536999367
21	22.389061300054532	27.287289561372706	28.780497949528495	21.54315118904427
22	22.307065656804788	27.38759702157087	28.81612127510808	21.489216046516262
23	22.38161209046235	27.39857818398693	28.686928368563574	21.532881356987144
24	22.234171244968763	27.47383363672023	28.645422692878697	21.646572425432314
25	22.286016375320056	27.569195777246293	28.49770425519334	21.647083592240314
26	22.304492024047807	27.57180688556426	28.4595274143386	21.664173676049337
27	22.318540798636775	27.532987513163626	28.391728739502597	21.756742948697003
28	22.305074919758603	27.588481160748877	28.424373369204144	21.682070550288376
29	22.378403694155903	27.601250528650596	28.303010036402082	21.717335740791416
30	22.349036617879037	27.537132000463256	28.324016511064386	21.789814870593325
31	22.460636447525296	27.49772232266059	28.20329288160595	21.838348348208164
32	22.477330481886554	27.578421269943526	28.133498529929607	21.810749718240316
33	22.44328632574072	27.49748305044851	28.10165961831876	21.957571005492017
34	22.43296096643422	27.60937081813392	28.023320295831965	21.93434791959989
35	22.458983835337083	27.561579294830977	28.05421513541718	21.925221734414755
36	22.513478615826436	27.524671462521265	28.035187482466796	21.926662439185503
37	22.52981332493173	27.483308003303836	28.02557884693201	21.961299824832427
38	22.469876332500213	27.514601599302818	28.092510587893056	21.923011480303916
39	22.465521903948815	27.438249670880037	28.06445996675963	22.031768458411516
40	22.46915109147626	27.537842440068967	28.047304479542817	21.94570198891196
41	22.52768678534659	27.476965584100103	28.023057671571962	21.972289958981342
42	22.414299738949722	27.409829520641487	28.009362647914433	22.166508092494357
43	22.477303268171802	27.538001479323082	27.958188610457114	22.026506642048002
44	22.630510196994454	27.477176146394346	27.864873163819286	22.027440492791914
45	22.53550786975159	27.386561655795905	27.926024846314455	22.15190562813805
46	22.442227676251946	27.544941919852768	27.907545016019792	22.105285387875497
47	22.498573465296037	27.55534968236832	27.86375189952473	22.082324952810918
48	22.4452507235986	27.473918265234698	27.867629808196536	22.21320120297017
49	22.56568364021928	27.495911888101215	27.78853294461105	22.149871527068456
50	22.469301556685526	27.31284991828241	27.822176790623125	22.395671734408936
51	22.50436587237583	27.439523637546426	27.83574597195585	22.220364518121897
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	43.667187027355645
2	41.11957023264007
3	40.86654739639706
4	41.697604675094766
5	45.68247788571475
6	42.248300877527264
7	45.89547193027421
8	42.22777356289841
9	41.359997453042126
10	41.9614198618305
11	46.109697462793555
12	42.0484293961519
13	44.74300090919577
14	44.20533123826784
15	44.540113437927054
16	44.59935148426216
17	44.53201456715245
18	44.37855230861854
19	43.84981919241923
20	43.7509146337623
21	43.9322124890988
22	43.79628170332105
23	43.914493447449495
24	43.88074367040108
25	43.933099967560366
26	43.96866570009714
27	44.07528374733378
28	43.987145470046976
29	44.09573943494732
30	44.138851488472355
31	44.29898479573346
32	44.28808020012687
33	44.400857331232736
34	44.36730888603411
35	44.38420556975184
36	44.44014105501194
37	44.49111314976416
38	44.392887812804126
39	44.49729036236033
40	44.41485308038822
41	44.49997674432793
42	44.58080783144408
43	44.50380991021981
44	44.65795068978637
45	44.687413497889644
46	44.54751306412744
47	44.580898418106955
48	44.658451926568766
49	44.71555516728773
50	44.864973291094465
51	44.724730390497726
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	77360.0
1	46165.0
2	14970.0
3	18098.5
4	21227.0
5	51265.5
6	81304.0
7	137725.5
8	194147.0
9	119853.0
10	45559.0
11	48869.0
12	52179.0
13	60630.5
14	69082.0
15	93306.5
16	117531.0
17	165345.5
18	213160.0
19	263602.5
20	314045.0
21	368162.0
22	422279.0
23	503893.0
24	585507.0
25	689243.5
26	917981.0
27	1042982.0
28	1135045.0
29	1227108.0
30	1315006.0
31	1402904.0
32	1517158.0
33	1631412.0
34	1733130.5
35	1834849.0
36	1988388.0
37	2141927.0
38	2241428.0
39	2340929.0
40	2438428.5
41	2535928.0
42	2587047.5
43	2638167.0
44	2676926.5
45	2715686.0
46	2702945.5
47	2690205.0
48	2657932.0
49	2625659.0
50	2535997.0
51	2446335.0
52	2311932.0
53	2177529.0
54	2022725.5
55	1867922.0
56	1706824.0
57	1545726.0
58	1382520.0
59	1219314.0
60	1080493.5
61	941673.0
62	807908.5
63	674144.0
64	578331.0
65	482518.0
66	403730.0
67	324942.0
68	291010.5
69	257079.0
70	226205.0
71	195331.0
72	165335.5
73	135340.0
74	112849.5
75	75081.5
76	59804.0
77	55251.5
78	50699.0
79	51487.5
80	52276.0
81	45656.5
82	39037.0
83	34446.5
84	29856.0
85	26874.5
86	23893.0
87	28277.0
88	32661.0
89	21148.5
90	9636.0
91	5614.5
92	1593.0
93	1090.0
94	587.0
95	352.0
96	117.0
97	82.0
98	47.0
99	30.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025847472938387406
2	3.062672378748245E-4
3	4.915095188475006E-4
4	0.03284963115915456
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0031046606291020517
12	0.0
13	0.002931767833527554
14	0.0
15	0.0
16	7.310895355721617E-4
17	0.007431920312623765
18	0.0015856739251261076
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0017536269265413338
25	7.409691238907044E-6
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	3.704845619453522E-5
34	5.162084896438573E-4
35	9.879588318542725E-6
36	1.2596475106141976E-4
37	0.0
38	0.0
39	0.0
40	2.5686929628211083E-4
41	0.006841614910590837
42	0.0016795300141522632
43	1.975917663708545E-4
44	0.001207779671941848
45	0.0019462788987529168
46	0.004080269975558146
47	0.007528246298729557
48	0.007545535578287006
49	0.0
50	0.016392706917542015
51	0.0627008072636314
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4.0487517E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	34.1248995158064
#Duplication Level	Relative count
1	100.0
2	15.427565999049301
3	5.6353716592526375
4	2.9400705219618444
5	1.8914642885015147
6	1.3536811187025095
7	1.0039092153899638
8	0.8059652782212006
9	0.637819353099348
10++	12.199448027300654
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	118267	0.2921073179172731	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	22224850	5.9540434	7.656367	18
AAAAA	11158730	3.8994997	4.1863093	45
TGTGT	7732875	3.3885794	39.950283	4
TGTTT	7240215	2.4807122	31.344193	6
GTTTG	5478360	2.400641	39.26574	7
CTTTG	5186260	2.3724613	41.164158	1
GTGTT	5130845	2.2483582	39.237892	5
TTTGA	6090240	2.200612	32.613586	8
GAGGG	2841580	2.1479502	11.3781	11
TTTGT	6069095	2.0794516	31.138437	2
TTGTG	4705610	2.0620184	39.295086	3
TGAGG	3162485	1.8691366	14.369916	10
TTGAG	3823120	1.7667648	23.438133	9
AGGGG	2132515	1.6119679	6.392594	12
TGAGC	2331380	1.438447	5.530727	10
GAGTG	2070940	1.2239962	5.3953853	11
TGAGT	2550875	1.1788268	6.7447796	10
TTGAT	2958560	1.069029	8.845723	9
TGATT	2769520	1.0007223	5.638653	10
TTGAC	1867855	0.90109843	6.260845	9
>>END_MODULE