FastQCFastQC Report
Mon 21 Jul 2014
3L22C_CGATGT_L004_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 3L22C_CGATGT_L004_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 13621119
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 182454 1.3394934733335786 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 113205 0.8310991189490379 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 104487 0.7670955668179684 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 83988 0.6166013232833514 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 71726 0.5265793507860845 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 69034 0.5068159231264333 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 61887 0.45434593149065067 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 58952 0.4327985094323014 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 57922 0.4252367224748569 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 57033 0.4187100927611013 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 47118 0.34591871636977845 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 43652 0.3204729361809408 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 38919 0.2857254238803728 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 37510 0.27538119298421815 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 36925 0.271086391654019 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 36897 0.2708808285134283 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 33874 0.24868735087036536 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 33489 0.2458608576872429 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 32121 0.23581762996123887 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 31794 0.2334169461407686 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 31427 0.23072260069088302 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 29782 0.21864576618117793 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 29590 0.21723619035998437 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 29356 0.21551826982790473 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 27476 0.20171617324538463 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 26834 0.19700290409326868 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 24791 0.18200413637088111 No Hit
GGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCAT 24439 0.1794199140320263 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 24351 0.17877385844731258 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 23935 0.17571977750139325 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 23092 0.16953085866146533 No Hit
GCTCATGGTAAGGCTTTATATGGTTTTGATGTTCTTTTATCATCTGCAGA 22616 0.166036285271423 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 22558 0.1656104759087708 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 22243 0.16329789057712513 No Hit
ATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAA 21813 0.1601410280609104 No Hit
GCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATG 20459 0.15020058190520177 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 20328 0.1492388400688666 No Hit
TACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGA 18100 0.13288188731043316 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 17962 0.1318687546889503 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 17628 0.12941668008333235 No Hit
TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC 17473 0.128278741269348 No Hit
GTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGG 17208 0.1263332329744715 No Hit
ACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGACTG 16591 0.12180350234074014 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 16343 0.11998280023836515 No Hit
GCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGA 15973 0.11726643016627342 No Hit
GGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTA 15851 0.11637076219655669 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 15785 0.1158862205080214 No Hit
CCACGTAGTTGGTTAGCTACTTCTCACTTTGTATTAGGATTTTTCTTCTT 15776 0.11582014664140296 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 15567 0.11428576462770791 No Hit
TTCATGCGTTGGAGAGACCGTTTCTTATTCTGTGCAGAAGCTATTTACAA 15511 0.11387463834652645 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 15139 0.11114358519296395 No Hit
ATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCATCTC 14901 0.10939629849794279 No Hit
GGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTATTT 14753 0.1083097504691061 No Hit
GCGACTGGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGT 14715 0.10803077192116153 No Hit
CTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGT 14659 0.1076196456399801 No Hit
GTATGGGCTTGGATGTTCTTATTTGGACATCTTGTTTGGGCTACTGGGTT 14509 0.10651841452967263 No Hit
GTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAG 14314 0.10508681408627293 No Hit
CCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCCAGAA 14026 0.10297245035448262 No Hit
GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA 13917 0.10217222241432587 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 5142205 3.6123972 5.6283603 45-49
CTGCT 4339770 3.5655546 7.4615707 5
ACTTC 4508110 3.417806 6.562173 60-64
GGAAG 3508650 3.1369574 6.082311 85-89
AACTT 5069360 3.1061718 5.6468115 55-59
AGCAC 2616740 3.0498266 6.6907673 90-94
AGCTG 3550365 3.0428984 7.3780484 3
GAAGA 3542995 2.923019 5.943836 90-94
CTACT 3818480 2.8949656 8.747334 2
CTGGT 3823860 2.7516258 13.427883 1
CTTCA 3593150 2.7241328 6.5970273 60-64
TCTCT 4247325 2.7035933 8.07491 20-24
TTCAA 4282665 2.6241372 8.157463 9
GCTGC 2472090 2.6215565 7.7611456 3
CTTCT 4064390 2.5871477 5.2109756 20-24
CGGAA 2521700 2.5741568 6.781033 95-97
CAACT 2804365 2.5322962 8.517806 9
TGGTG 3998055 2.5197833 8.981922 6
CTGAA 3176570 2.5122647 5.7757044 80-84
GGTGT 3965975 2.4995644 10.372946 1
ACAAC 2319190 2.4942696 6.1968203 85-89
GGTGG 3062850 2.491579 10.701088 7
CGGTG 2679715 2.4889176 12.288733 9
TATGC 3627400 2.4086592 6.9161224 30-34
ACCCA 1793820 2.387077 29.742605 1
CAACA 2212970 2.3800309 6.0240545 85-89
AGGAA 2879835 2.375903 5.6047974 40-44
ATGCA 2998545 2.3714695 6.5192966 1
CCGTG 2213155 2.346966 6.076879 60-64
CAAGC 2013020 2.346187 6.5582886 75-79
TCTGC 2831745 2.3265617 5.7123146 85-89
AGAGC 2259020 2.3060124 7.5005236 95-97
TCAAC 2524530 2.27961 7.5869036 8
GCAGC 1800545 2.2741861 6.3108983 1
CATGG 2649930 2.2711658 9.971773 2
ACATC 2509440 2.2659836 5.4734054 40-44
AAGAG 2721690 2.2454312 5.282432 90-94
GAAGC 2198975 2.2447183 6.0941987 15-19
CGTGA 2618835 2.2445154 8.122073 9
ATCGG 2618450 2.2441854 5.8287263 95-97
TCGGA 2611640 2.2383487 5.5790563 95-97
CAGCA 1915325 2.232323 5.4125175 2
ATGGT 3780885 2.198874 5.3147316 5
GCTGG 2349855 2.182544 8.541871 4
GAGCA 2135510 2.179933 6.2979727 90-94
CCAAG 1864160 2.17269 6.3812394 75-79
TCCTT 3405195 2.1675434 6.2060065 40-44
GATCG 2526890 2.1657124 6.2977233 95-97
ACTGC 2205350 2.1580675 7.3814335 4
GCACA 1808505 2.1078236 6.875013 95-97
CTGCA 2138295 2.0924501 5.106754 95-97
CAAGG 2016190 2.058131 5.7974114 10-14
CAGCT 2093855 2.0489626 5.255641 2
CTTGG 2813860 2.0248363 6.40956 2
GGCTC 1901545 2.0165155 5.683669 10-14
CTCTG 2450795 2.013573 6.3810678 45-49
CCTTA 2646580 2.0064943 6.511385 45-49
CTCTC 2134770 2.0025551 5.347442 15-19
GCTAC 2030615 1.9870787 10.948697 1
CACAC 1489720 1.9824041 7.902932 95-97
TAGCT 2955190 1.9622996 5.399039 2
AGATC 2479550 1.9610103 5.8133836 95-97
TGGCT 2709965 1.950074 8.122235 9
TATTC 3750325 1.9293642 6.3183184 2
CATCC 1696790 1.8957827 6.111821 90-94
TGCTG 2618660 1.8843714 5.7071567 6
TTCCA 2479415 1.879759 5.05722 25-29
GTGGC 2022870 1.8788407 9.749017 8
CCAGC 1301120 1.8763438 6.5020437 1
CTCTA 2470265 1.8728217 5.4340796 95-97
AAGGA 2233685 1.8428204 5.1432214 10-14
GCATG 2143455 1.8370831 5.723151 3
CTCCT 1957570 1.8363298 5.541597 70-74
TTCGG 2538935 1.827002 9.378414 7
CACAA 1688710 1.8161936 6.132432 4
TTTCA 3517645 1.8096614 7.0478344 8
GCAGG 1630580 1.8038114 5.713186 90-94
CACCC 1093475 1.8004328 7.1606827 50-54
GCTTC 2157575 1.7726635 6.884138 15-19
GCTCT 2153005 1.7689087 5.7330894 10-14
TACTG 2660800 1.7668195 5.1739717 3
TCGGT 2448925 1.7622311 10.439185 8
GCACC 1198070 1.7277354 6.4684324 50-54
TGAAC 2180590 1.7245705 5.3935466 80-84
AATGA 2690335 1.7196214 5.5736594 75-79
TTGGT 3511355 1.7145672 13.206518 7
CCTGT 2078040 1.7073175 7.368113 1
CCACA 1270360 1.6904969 6.4013467 1
GTGTA 2887420 1.6792558 9.339558 2
ACCAG 1434030 1.6713707 5.1175056 2
CCCAA 1239710 1.6497103 29.731558 2
CATGC 1685730 1.649588 5.419821 2
TGTAG 2827275 1.6442767 5.9450207 3
GTCTG 2267460 1.6316501 6.085902 90-94
CGCAG 1288715 1.6277171 6.574522 90-94
GTAGC 1897110 1.6259491 6.1435685 1
GCTTG 2237030 1.6097529 6.0543714 1
CTTTC 2516640 1.6019424 8.309872 7
TGGTA 2725595 1.5851421 9.945312 2
CCAAT 1718330 1.5516242 20.394205 3
GAACA 1625140 1.5308226 5.1811433 80-84
TACTT 2967880 1.5268335 7.420682 5
CCGCC 853765 1.5233997 6.431353 45-49
ACACA 1405500 1.5116036 5.498276 3
ACACG 1292725 1.5066789 6.765357 95-97
ATGCC 1494330 1.4622916 6.4372516 9
AACAC 1359520 1.4621525 5.516295 85-89
GCTGT 2013760 1.4490892 5.8782134 7
ACGGT 1683840 1.4431626 6.280048 4
ACATG 1787065 1.4133421 9.125697 1
GTATT 3120420 1.4060001 5.907916 4
AGCCT 1423560 1.393039 6.0371547 2
GGTAC 1603885 1.3746359 11.712283 3
GGTCA 1600240 1.3715119 18.711979 9
TGTAT 2968750 1.3376606 5.692487 3
ACTTT 2593960 1.3344694 7.268512 6
CACGT 1363515 1.3342813 5.077172 95-97
AACGC 1140925 1.3297551 5.501292 95-97
GACGG 1190380 1.316845 8.077937 3
TGGTC 1805945 1.299547 15.9231 8
GCCTG 1203815 1.2765995 5.370015 3
GCGGC 926115 1.2676357 5.604527 7
ATGGG 1685130 1.2649543 8.626344 3
ATTCG 1853420 1.2307043 7.676114 6
GGTCG 1288975 1.1971992 7.8924255 6
CAATT 1951230 1.195586 13.98144 4
AAGCC 1022210 1.1913921 5.8879976 1
TGGGT 1857085 1.170432 6.3075156 4
GTACT 1741605 1.1564572 9.050667 4
CTGAC 1181005 1.1556844 7.092091 1
TGACG 1313360 1.1256367 6.1599407 2
ATTGG 1876395 1.0912672 13.036406 6
GGGTC 1157350 1.0749459 7.9086394 5
TGGCC 1001880 1.0624553 8.356701 1
TGGGG 1230525 1.0010123 5.180135 8
TCGTG 1374165 0.9888406 6.191211 8
CGGTA 1079450 0.9251603 5.7813344 5
AATTG 1718155 0.9220654 11.22339 5
GGGGT 1109940 0.9029183 5.062978 8
GCCTA 786955 0.77008265 5.7643123 3
GGCCT 696200 0.7382934 5.123189 2
GTCGT 966175 0.6952535 5.9542823 7
Produced by FastQC (version 0.10.1)