FastQCFastQC Report
Mon 21 Jul 2014
3L22B_CGATGT_L003_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 3L22B_CGATGT_L003_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 11947813
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 159663 1.3363366165841397 No Hit
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 158365 1.325472703665516 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 124207 1.0395793774140925 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 120567 1.0091135507393696 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 96334 0.8062898205721835 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 69016 0.5776454653249092 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 68203 0.5708408727187143 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 67394 0.5640697590429311 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 60008 0.5022509140375733 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 59720 0.49984043104792486 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 51486 0.4309240527952689 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 50291 0.4209222223347487 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 46461 0.3888661464654661 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 46141 0.38618783203252344 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 44449 0.3720262444683391 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 43107 0.36079406331518576 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 42096 0.3523322636536076 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 41862 0.3503737462245182 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 39768 0.3328475261539497 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 39486 0.330487261559919 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 39260 0.32859570199165317 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 34701 0.29043809105482316 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 34644 0.28996101629645527 No Hit
GGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTA 33411 0.27964113599702306 No Hit
GGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTATTT 30243 0.25312582311089066 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 30091 0.2518536237552429 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 29644 0.24811235328172612 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 28585 0.2392488064552065 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 28475 0.23832813586888243 No Hit
CGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTC 27294 0.2284434816648034 No Hit
GTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCATCTCTGCT 24721 0.20690815967742382 No Hit
ATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCATCTC 24656 0.20636412705823232 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 24499 0.20505007903956984 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 24102 0.20172729519620033 No Hit
GGTCCTTACGAGTTAATCGTTCTTCACTTCTTACTTGGTGTAGCTTGCTA 22455 0.18794234559914855 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 22427 0.1877079930862661 No Hit
GTATGGGCTTGGATGTTCTTATTTGGACATCTTGTTTGGGCTACTGGGTT 22029 0.18437683951029366 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 21983 0.18399183181055814 No Hit
TACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGA 21863 0.18298746389820464 No Hit
ATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGATTTCACTAAA 21794 0.18240995234860136 No Hit
TAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTA 21725 0.1818324407989981 No Hit
GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA 20866 0.17464284049306764 No Hit
GTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGG 20422 0.1709266792173597 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 20396 0.17070906616968312 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 20208 0.1691355564403293 No Hit
GGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCAT 20008 0.16746160991974013 No Hit
GTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAG 19852 0.16615593163368056 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 18861 0.15786152662416125 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 18730 0.15676509165317537 No Hit
GTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCTTTCAA 18176 0.15212825979114336 No Hit
GGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCTGGTACTTT 18025 0.15086443016809856 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 16541 0.13844374698532694 No Hit
CTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGT 16261 0.1361002218565021 No Hit
CGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTG 16255 0.13605000346088444 No Hit
TGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTATT 15665 0.1311118612251464 No Hit
GCGACTGGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGT 15058 0.12603143353515828 No Hit
CGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGGATCGCTGT 15055 0.12600632433734943 No Hit
CGTGGTGGACTTGATTTCACTAAAGATGATGAAAACGTAAACTCTCAACC 14293 0.11962858809390473 No Hit
GCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTC 14211 0.11894227002046316 No Hit
TACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAAC 14081 0.11785420478208021 No Hit
CATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGC 13752 0.11510056275571103 No Hit
ATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTG 13319 0.11147646853863548 No Hit
GCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGA 13241 0.11082362939560571 No Hit
GCTCATGGTAAGGCTTTATATGGTTTTGATGTTCTTTTATCATCTGCAGA 13125 0.109852740413664 No Hit
CCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCATCTCTG 12912 0.10806998736923652 No Hit
GGCCCTGGAGATTTCTTAGTTCATCACGCTATTGCTTTAGGTTTACATAC 12649 0.10586874769466179 No Hit
GGGTAACTTATTATTATTTTACAAACCAAGATTTACCATGACTGCTACTT 12611 0.10555069785574983 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 12276 0.10274683743376298 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
ACTTC 4183600 3.6891022 6.3976727 40-44
CTGCT 3766230 3.5043533 17.807201 5
AAAAA 3863355 3.292392 7.2084537 45-49
AACTT 4556780 3.2568736 5.332157 55-59
CTACT 3528340 3.1112933 17.3351 2
CAACT 2907830 3.1099641 7.4812016 9
TCAAG 3089710 2.8262267 5.1420836 30-34
CTTCT 3855780 2.8032823 9.474376 40-44
CTTCA 3141520 2.7701948 5.799058 60-64
TTCAA 3852910 2.7537954 7.6503944 9
ACTGC 2420970 2.7321613 19.433949 4
TCTCT 3749610 2.7260933 7.698852 20-24
AGCTG 2786675 2.6897142 8.68462 3
TCAAC 2499785 2.6735547 6.7286215 8
CTGGT 3324610 2.6457248 13.096632 1
AGGAA 2780615 2.63845 5.960935 40-44
AGCAC 1913105 2.6186144 5.6134334 35-39
ACCCA 1597105 2.5560145 29.120914 1
GCAGC 1765920 2.550553 9.431394 65-69
CTGCA 2218080 2.5031917 7.6206756 35-39
GCTGC 2094095 2.493698 8.207221 3
GGTTC 3129810 2.4907026 11.308617 3
GAAGC 2081525 2.4367855 6.030797 35-39
ACAAC 1871605 2.4278197 5.710979 85-89
CGGTG 2374505 2.4183738 16.60088 9
CAGCA 1751750 2.3977554 5.88373 35-39
CAACA 1796285 2.3301156 5.6892104 85-89
GGTGT 3406450 2.3185093 12.703076 8
CAAGG 1979980 2.3179095 5.843474 4
CCGTG 1930335 2.2986884 7.7150364 95-97
AAGCT 2510660 2.2965567 5.019574 35-39
ATGGT 3555075 2.2931108 11.80295 1
TATGC 3023465 2.2802281 6.646486 30-34
GGTGG 2594810 2.260262 9.342793 7
CAAGC 1637175 2.2409277 6.1368074 1
TCTGC 2405925 2.2386339 5.8622417 85-89
CTCCT 2048395 2.2284958 10.424809 70-74
CTCTG 2387910 2.2218719 8.420261 45-49
ACATC 2070285 2.214198 5.4743247 40-44
TGGTG 3252315 2.2136018 8.724289 6
TGCAG 2277485 2.1982412 7.894162 65-69
CAGCT 1909810 2.1552966 7.435259 65-69
CGGAA 1820740 2.1314914 6.2119246 95-97
GCTCC 1519845 2.1161373 9.686108 70-74
CCAAG 1540445 2.1085258 5.8674126 75-79
CGTGA 2179595 2.1037571 8.085595 9
CATGG 2172245 2.096663 10.090242 2
TGGTT 3921945 2.0857484 7.802811 2
ATGCA 2273195 2.0793421 6.2544804 1
GCTAC 1839055 2.0754468 22.16554 1
TTTCA 3506605 2.0663972 6.387387 8
GATGG 2481915 2.0488474 5.208263 1
ATCCG 1793315 2.0238276 9.263237 95-97
GTTCC 2132905 1.984598 5.657591 95-97
AAGGA 2075905 1.9697698 5.055308 35-39
AGCTC 1745320 1.9696633 7.7350893 70-74
TCCTT 2702215 1.9646019 6.1892524 40-44
CCTAC 1488665 1.9643143 7.3743453 20-24
CTCTA 2222045 1.9594012 8.093186 25-29
CCCAA 1204375 1.9274875 29.697851 2
GCTGG 1888345 1.923232 10.512283 4
GCAAC 1401310 1.918081 8.057918 35-39
TGCTG 2403380 1.9126099 13.9073925 6
CCAGC 1130100 1.9084345 6.740723 3
CTTTC 2621395 1.9058428 7.585306 7
CCTGT 2040440 1.8985622 9.274313 1
AATGA 2554390 1.893864 6.51112 75-79
GCAGG 1501135 1.8543255 5.70773 90-94
GCTTC 1975375 1.8380215 6.6162424 15-19
CTTGG 2305540 1.8347486 7.7639403 2
TCCGT 1963350 1.8268325 7.211551 95-97
TTCCA 2061130 1.817506 5.8660717 95-97
CTGTA 2405935 1.8145012 8.121627 8
CCTCC 1109165 1.8056674 6.804479 70-74
TTCGG 2268165 1.8050057 12.826167 7
CTCTC 1658595 1.8044236 5.2221317 15-19
TTCCT 2475730 1.7999394 5.521937 20-24
CACAA 1380255 1.7904474 5.35199 85-89
CCTTA 2026635 1.7870885 5.792351 45-49
GAAAA 1982750 1.7829758 5.61244 40-44
CTGTT 2863270 1.7804092 10.99626 8
CATCC 1349035 1.7800707 5.357792 90-94
GGAGA 1761890 1.7640762 5.585791 7
CCAAT 1647115 1.761612 19.88218 3
TGGCT 2212950 1.7610657 6.45653 9
TACTG 2332965 1.759469 13.14087 3
TGGTA 2716490 1.7522029 9.883298 2
GGCTC 1469285 1.7496593 5.599235 1
GATCG 1809540 1.7465781 5.396144 95-97
ATGAT 2844965 1.7390925 6.166164 15-19
TCGGT 2182515 1.7368456 14.56482 8
TAGCT 2292855 1.7292191 6.049628 2
GCACA 1247540 1.7076042 5.0654407 95-97
CTATT 2873615 1.6933845 5.391228 25-29
TATTC 2865990 1.688891 7.57983 5
TGCAA 1840135 1.6832125 6.2627125 35-39
CACCC 846090 1.6706059 6.62362 50-54
ACCAG 1210995 1.6575823 6.0694165 30-34
ATTGC 2179835 1.6439817 5.178379 55-59
AGATC 1794815 1.6417572 5.4290543 95-97
TGTAG 2542945 1.6402621 6.942958 3
GCACC 964020 1.6279701 5.9242725 50-54
ATTCC 1839805 1.6223414 5.9721413 20-24
GTGGC 1592460 1.6218805 7.656625 8
CTACC 1223445 1.6143528 7.374601 20-24
CTATG 2137825 1.612299 5.470303 4
GCATG 1669335 1.6112514 5.650105 3
CGCAG 1112470 1.6067622 6.6403966 90-94
CCGCC 767945 1.5999933 8.815611 45-49
ATGCC 1415960 1.5979674 6.2817764 9
CCACA 985460 1.577135 5.684039 60-64
TTCAT 2663810 1.569749 6.0218034 60-64
CACAC 977545 1.5644677 5.9710116 95-97
TACTT 2654260 1.5641214 6.8488245 5
ACGGT 1614740 1.558556 6.0423503 4
GCTGT 1954750 1.5555902 14.150271 7
TTGGT 2907210 1.5460974 10.676443 7
CAACC 953875 1.5265862 5.1236873 75-79
GGTAC 1567825 1.5132732 11.466374 3
TTGCA 1990130 1.5009105 5.2943797 65-69
GACGG 1198985 1.481085 7.927167 3
GTGTT 2736885 1.455516 7.9715858 9
ACACA 1115430 1.44692 5.0756707 85-89
GCTTG 1813695 1.4433386 7.429568 1
GCACT 1275510 1.4394639 5.882921 35-39
TGTTT 3457275 1.43664 8.220716 9
AAACC 1106485 1.4353167 5.0981765 40-44
GTAGC 1483935 1.4323022 7.5283413 1
TCCTG 1529895 1.423517 6.461339 75-79
AGCCT 1254775 1.4160635 8.267336 1
GGTCA 1461155 1.4103148 16.460463 9
GTGTA 2185845 1.4099238 11.171418 2
GTTTT 3370700 1.4006646 5.006347 10-14
ACTCT 1585865 1.3984171 5.6343455 25-29
GTATT 2754140 1.3880844 6.874101 4
TCTAT 2339285 1.3785106 5.275596 25-29
AACAC 1057245 1.3714433 5.083221 85-89
GGCTG 1346435 1.3713102 6.084421 2
GTCTG 1717825 1.3670453 5.6439085 90-94
CAGGT 1414775 1.3655487 5.6492534 95-97
ACTTT 2314275 1.3637726 6.692787 6
AACCA 1044475 1.3548782 5.293081 75-79
GCCTG 1104405 1.3151515 9.445341 6
CAATT 1838175 1.3138012 13.323543 4
AACGC 958305 1.3117061 5.2287664 90-94
TCCTC 1200835 1.3064159 6.3062096 70-74
TGTAT 2580900 1.3007716 6.715966 3
TGGTC 1632270 1.2989606 13.669088 8
ACATG 1408175 1.288089 8.770399 1
CTGAC 1141180 1.2878672 6.7939835 1
GCGGC 842720 1.2843314 7.457312 7
AGGTA 1633840 1.2782085 7.1795664 6
GTACT 1693840 1.2774556 8.694991 4
ATGGG 1529415 1.2625486 9.689945 3
ACCGC 745810 1.2594723 5.07915 45-49
CAGGA 1064390 1.2460529 5.287115 5
TGACG 1285385 1.2406608 5.856994 2
AAGCC 882125 1.2074326 8.583288 1
TACCA 1120915 1.1988341 5.1453304 30-34
GGTCG 1165630 1.1871648 8.149926 6
ACACG 864200 1.1828972 5.138911 95-97
TACTC 1329255 1.1721382 5.280419 25-29
TAGGT 1803325 1.163189 5.517518 5
GTAGA 1479655 1.1575844 6.306597 4
TACCC 869110 1.1468029 5.791459 20-24
ATTGG 1771045 1.1423676 11.575234 6
TATGG 1752710 1.1305411 5.063016 5
GCGAC 778405 1.1242656 6.35367 20-24
TGGGT 1647420 1.1212726 6.1784987 4
TGGCC 927080 1.1039888 10.102452 1
GTTCG 1380790 1.0988327 10.886035 4
TCCTA 1235505 1.0894694 5.574096 20-24
ATTCG 1431435 1.0795555 9.572747 6
CGGTA 1101690 1.0633574 5.4965773 5
CGCCT 758120 1.0555589 5.7058544 45-49
GGGTC 1027855 1.0468445 9.459898 9
CCAGG 724385 1.0462434 5.745522 2
GGCAG 827850 1.0226283 5.0247917 3
AATTG 1659775 1.0146003 10.566367 5
TTGGC 1258985 1.0019002 6.419009 3
TCGTG 1242535 0.9888094 6.2940555 8
TGGGG 1126515 0.98127383 7.5098877 7
TGCGG 955065 0.9727098 5.278857 6
CGGCT 775820 0.9238648 5.6166973 8
GGCCA 638370 0.92201036 5.2527914 1
GCCCA 532220 0.89877623 6.490946 2
GGGGT 1009545 0.8793847 7.3815045 8
CCCTA 625090 0.82481503 7.0452237 2
GGCCC 455840 0.8122755 8.881452 1
CAGGC 550715 0.7954085 5.0409403 1
AGGCC 542610 0.7837022 5.0900445 1
GGCCT 646910 0.7703557 9.387601 5
GCCTA 678255 0.7654378 7.827213 2
GCCCT 529570 0.7373402 7.9019494 1
GTCGT 859650 0.6841095 6.1334825 7
CCTAT 735055 0.64817214 5.9551187 3
GGTAA 781450 0.6113548 6.400362 7
CCTAG 367625 0.41487947 5.800084 3
CTAGG 422460 0.40776074 5.163778 4