FastQCFastQC Report
Mon 21 Jul 2014
3L22A_CGATGT_L002_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 3L22A_CGATGT_L002_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 11382017
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[OK] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 257877 2.2656529154718363 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 110197 0.9681675927913305 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 87304 0.7670345247243965 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 83538 0.7339472432698 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 75299 0.6615611275224769 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 61887 0.5437261251674461 No Hit
GACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCT 57043 0.5011677631477796 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 55747 0.48978138057604376 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 49016 0.43064423467299334 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 43453 0.3817688903469394 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 42769 0.3757594106563011 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 42484 0.3732554607852018 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 39966 0.351132844029314 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 35743 0.3140304569919374 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 34376 0.3020202833996821 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 33338 0.2929006343954679 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 33312 0.2926722038809114 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 32148 0.2824455454600006 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 30299 0.26620062155943014 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 29761 0.26147386706591635 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 29664 0.26062164553084044 No Hit
TGACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTC 29459 0.2588205587814532 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 29011 0.25488452529986555 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 28200 0.24775925040350935 No Hit
ACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGACTG 26351 0.23151432650293882 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 26339 0.231408897034682 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 25679 0.2256102762805573 No Hit
TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC 25066 0.22022458761043845 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 23209 0.2039093773976967 No Hit
GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA 22595 0.1985149029385565 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 21468 0.18861331871143752 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 20880 0.1834472747668537 No Hit
ACCCAATTTGGGAAGCTGCTTCCGTTGATGAATGGCTTTACAACGGTGGT 20740 0.18221726430385757 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 20253 0.17793858505043525 No Hit
CCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCCAGAA 19535 0.1716303885330693 No Hit
GCTCATGGTAAGGCTTTATATGGTTTTGATGTTCTTTTATCATCTGCAGA 19161 0.16834450343906532 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 18861 0.165708766732645 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 18691 0.16421518259900683 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 18223 0.16010343333699115 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 17893 0.1572041229599288 No Hit
CTGCTGCTATCATCCCTACTTCTGCAGCTATCGGTTTGCACTTCTACCCA 17889 0.1571689798038432 No Hit
GCTAAAAACTATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGA 17685 0.1553766788434774 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 17437 0.15319780316616993 No Hit
CCACGTAGTTGGTTAGCTACTTCTCACTTTGTATTAGGATTTTTCTTCTT 17337 0.15231922426402983 No Hit
TACAACATCGTAGCTGCTCACGGTTACTTTGGTAGATTAATTTTCCAATA 16688 0.14661724718914054 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 16207 0.14239128266984666 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 15802 0.13883303811617922 No Hit
GTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATATTGATGGTAT 15791 0.13873639443694383 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 15770 0.13855189286749442 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 15400 0.135301150929576 No Hit
TACCATGACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTC 15151 0.13311348946324714 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 14960 0.13143540376015955 No Hit
GTATGGGCTTGGATGTTCTTATTTGGACATCTTGTTTGGGCTACTGGGTT 13635 0.11979423330680317 No Hit
TTCATGCGTTGGAGAGACCGTTTCTTATTCTGTGCAGAAGCTATTTACAA 13562 0.1191528707082409 No Hit
CATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGC 13467 0.1183182207512078 No Hit
GTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAG 12973 0.11397804097463568 No Hit
GCGACTGGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGT 12591 0.1106218695684605 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 12586 0.1105779406233535 No Hit
ATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTG 12494 0.10976964803338458 No Hit
GCTGCTATCATCCCTACTTCTGCAGCTATCGGTTTGCACTTCTACCCAAT 12076 0.10609718822243897 No Hit
ACCTCCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCC 11865 0.10424338673892333 No Hit
ATACACTTGGGTAACTTATTATTATTTTACAAACCAAGATTTACCATGAC 11778 0.10347902309406146 No Hit
GCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATG 11585 0.10178336581293104 No Hit
GCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCT 11425 0.10037763956950688 No Hit
GCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGT 11387 0.10004377958669364 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 4481995 3.9754236 5.6588817 90-94
CTGCT 3488050 3.386725 11.238931 4
ACTTC 3567935 3.294581 7.6556478 60-64
AGCTG 3016715 3.01863 6.8905783 80-84
AACTT 3962825 3.0159955 6.5660024 60-64
CTACT 2987870 2.7589576 10.953949 2
CTTCT 3531985 2.7427123 6.4349637 20-24
CAAGC 1976610 2.7137008 9.624386 75-79
GGAAG 2623735 2.7056642 7.4110985 15-19
CTTCA 2875990 2.6556487 7.576476 60-64
GCTGC 2186270 2.6542192 9.948597 3
CAACT 2395525 2.6303034 8.505854 55-59
TCAAG 2728140 2.5961413 6.3819423 10-14
TTCAA 3403035 2.5899553 7.9269342 55-59
TCTCT 3299770 2.562389 9.128507 20-24
ACAAC 1947015 2.5421214 9.266013 85-89
TGCTA 3159985 2.52886 6.441142 4
AGGAA 2572195 2.5225737 7.286443 10-14
ACCCA 1582845 2.507395 47.88664 1
CTGGT 2962015 2.4925332 10.414498 1
TCAAC 2197970 2.4133866 8.372096 55-59
AAGCT 2523740 2.4016306 6.53272 15-19
GCTAC 2053255 2.37062 14.063613 1
CTGAA 2489865 2.3693948 7.012703 80-84
GAAGC 1976395 2.351642 8.299021 15-19
CAGCA 1711100 2.3491802 6.436845 2
CAACA 1798035 2.3476057 8.823478 85-89
TGGTG 3189865 2.32639 7.0469766 8
ACTGC 2014245 2.3255804 8.812907 2
ACATC 2099180 2.3049145 7.4205146 90-94
CGGTG 2173205 2.2865999 11.2597065 9
GCAGC 1581645 2.2833047 7.886673 1
GGTGT 3107150 2.2660654 9.666124 1
CCAAG 1647435 2.261774 8.82334 75-79
GGTGG 2475335 2.2572517 6.5064116 7
TATGC 2817495 2.2547734 7.888244 30-34
TCCTT 2794905 2.1703432 6.4873185 40-44
CAGCT 1865490 2.153833 6.5195365 2
CAAGG 1802825 2.145117 7.8852215 10-14
TGAAA 2702630 2.11978 5.014741 65-69
CTTTA 3254945 2.08328 6.5003448 5
CATGG 2078970 2.0802896 14.812927 2
GCTTC 2131285 2.0693727 8.814851 15-19
CTTGG 2420455 2.0368109 6.504416 6
CCAGC 1215875 2.0252903 6.8425345 1
ATGGT 2914060 2.0211322 5.258979 1
CTCTG 2071925 2.011737 8.400408 20-24
CTCCT 1772270 1.9855031 7.816266 70-74
GCTGG 1886485 1.9849192 7.5695586 4
GCTCC 1405095 1.9682585 5.963012 65-69
CACAA 1496850 1.954363 8.191844 85-89
AAGGA 1970850 1.9328296 7.0902143 10-14
TTCCA 2092710 1.932379 6.4933906 75-79
GTTCC 1987925 1.9301776 5.918682 70-74
CGTGA 1920835 1.9220542 12.4293165 9
CCTTA 2059815 1.9020042 9.47692 95-97
CCCAA 1185700 1.878275 48.194405 2
TTTCA 2916905 1.8669225 6.4044476 8
AGCTC 1614530 1.8640828 5.238406 65-69
CATCC 1364755 1.8180981 8.360961 90-94
CCAAT 1600045 1.7568606 33.587944 3
GGTAT 2531420 1.7557412 5.925792 6
TATTC 2733340 1.7494344 6.259798 5
GCCTT 1780120 1.7284088 5.930961 35-39
CACCC 891690 1.7137825 6.077515 50-54
CTTTC 2196400 1.7055829 7.5481424 7
TTCGG 2010845 1.6921245 8.941088 7
TTGGT 2894460 1.6882704 19.276785 7
CCGCC 832875 1.6831995 5.817973 45-49
CCACA 1062320 1.682828 7.7207723 1
AGAAA 1795640 1.674732 7.6464734 9
TCTGG 1987160 1.6721935 5.5306873 45-49
TCGGT 1985390 1.6707041 10.860708 8
GCACC 999535 1.6649313 5.394217 50-54
CCTGT 1706020 1.6564615 6.519313 1
TACTT 2580645 1.651704 8.091056 3
GAACA 1448935 1.639579 7.5103955 80-84
AGCTT 2047825 1.6388252 5.491231 15-19
ACACA 1248050 1.6295173 7.918522 85-89
TCCAA 1461080 1.6042762 6.921872 75-79
GCTTG 1901250 1.5999004 6.289002 1
GTGGC 1514730 1.5937667 5.048933 8
TGTAG 2281400 1.5823326 5.30692 9
GCTGA 1558305 1.5592941 6.439346 80-84
AACAC 1191840 1.5561266 7.96741 85-89
ACGGT 1552970 1.5539558 8.688472 4
CATGC 1345005 1.5528982 6.3213296 2
TTTAG 2797100 1.5515597 5.355542 6
GTGTA 2232570 1.548465 8.973151 2
TGGTA 2230875 1.5472894 7.969146 2
TGAAC 1611705 1.533724 6.624837 80-84
CTACC 1146975 1.527976 5.119594 25-29
ACATG 1586100 1.5093579 15.087389 1
ACTTT 2347230 1.5023103 8.35426 4
TTAGG 2155805 1.4952224 6.193214 35-39
GTAGC 1488390 1.4893348 5.38734 1
CATGA 1546285 1.4714692 5.8739395 65-69
AACAT 1623295 1.4690789 5.7066007 90-94
ATGCC 1268235 1.4642619 9.325195 9
GCAAC 1066205 1.4637996 6.9141397 1
ATGGG 1666905 1.4455826 12.639862 3
AAGCC 1044890 1.4345361 6.3292923 1
GGTCG 1349010 1.4193996 12.853708 6
AGCCT 1225925 1.4154123 5.391864 4
TTCAT 2210650 1.4148942 5.4766507 60-64
CTGTA 1766085 1.4133554 6.5110097 8
GCGGC 927035 1.4072295 7.0736127 7
GGCTG 1319450 1.3882971 5.6382723 9
GACGG 1109120 1.3876818 10.952321 3
GGTAC 1380565 1.3814414 9.847745 3
ATCTC 1471345 1.3586193 6.1288004 45-49
GGAAT 1641290 1.3536408 5.370744 40-44
TCATG 1690345 1.3527426 5.0860543 60-64
TTCTC 1735735 1.3478601 5.642333 20-24
GTCAA 1415770 1.347269 6.0256767 10-14
CAATT 1766840 1.3446928 23.492619 4
GTATT 2358900 1.3084888 5.9796247 4
TGGGT 1789260 1.3049194 9.480952 4
TGGTC 1547380 1.302119 23.58268 8
GGGTC 1234670 1.2990935 12.810847 5
GGTCA 1287960 1.2887776 27.748325 9
GAATC 1352315 1.2868842 5.875458 40-44
GACTG 1271540 1.2723471 7.282435 1
TGCCT 1282595 1.2453367 5.8919654 30-34
ATCCT 1339670 1.2370323 5.7202353 90-94
CTGAC 1069865 1.2352307 9.927587 1
GTATG 1779290 1.2340792 6.0721145 7
TGTAT 2177825 1.2080462 5.8114996 3
GTACT 1504615 1.2041073 7.8518524 4
TGACG 1192515 1.193272 8.529831 2
CACAT 1068550 1.1732754 5.2140107 2
GCCTG 949915 1.1532348 6.3866954 6
TGGCC 927830 1.1264228 8.741206 1
ATTGG 1619710 1.1233978 20.033606 6
ATTCG 1385155 1.1085062 7.554574 6
TAGGA 1330855 1.097612 5.007959 40-44
GCTCA 946620 1.0929359 6.1297317 1
CTTAT 1706535 1.0922428 6.3652797 95-97
TGCGG 1037280 1.091404 5.172915 6
TCGTG 1285885 1.0820712 10.408144 8
AATTG 1564625 1.0320306 18.506817 5
TTATG 1800375 0.9986735 5.6858683 95-97
CGGTA 997565 0.99819815 8.190601 5
CGGCT 809455 0.9827107 5.4394155 8
TAGAA 1232780 0.96691823 6.1546564 8
GCCTA 794550 0.91736096 5.0598407 3
CTACA 763495 0.838323 7.127169 3
GGCCT 660850 0.8022983 6.171804 5
TTAGA 1206825 0.796025 5.4749346 7
GTCGT 936975 0.7884637 10.164111 7
GGCCC 418235 0.73254216 6.0192304 1
CCCCG 347160 0.70159334 5.561065 1
Produced by FastQC (version 0.10.1)