FastQCFastQC Report
Mon 21 Jul 2014
3L15C_GCCAAT_L004_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 3L15C_GCCAAT_L004_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 16180428
Filtered Sequences 0
Sequence length 101
%GC 46

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[OK] Per base sequence content

Per base sequence content

[WARN] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GCGAGTCGGGCTGTTTGGGAATGCAGCCCTAAGTGGGAGGTAAATTCCTT 330825 2.044599809102701 No Hit
GATACCGTCCTAGTCTCAACCATAAACGATGCCGACTAGGGATTGGCGGA 227019 1.4030469404146788 No Hit
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 190951 1.1801356552496634 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 125791 0.7774269011919832 No Hit
CAGACCTCTGATCAGGAAAGACTACCCGCTGAGTTTAAGCATATCACTAA 99322 0.6138403755450721 No Hit
CCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCCAGAA 74735 0.46188518622622343 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 74647 0.4613413192778337 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 73247 0.45268889055345135 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 51894 0.32072081158792587 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 50751 0.31365672156509083 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 50680 0.31321791982264 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 50105 0.3096642437394116 No Hit
CAACACGGGGAAACTTACCAGGTCCAGACATAGTAAGGATTGACAGATTG 50007 0.3090585737287048 No Hit
CATGGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAAC 49817 0.3078843155446815 No Hit
GACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCT 49202 0.304083427212185 No Hit
GATCAGGAAAGACTACCCGCTGAGTTTAAGCATATCACTAAGCGGAGGAG 48318 0.2986200365033607 No Hit
CAAACGAATAAGGGCTTATGGTGGATACCTAGGCACCCAGAGACGAGGAA 46264 0.2859256875034455 No Hit
TGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGT 46087 0.28483177330043435 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 44521 0.2751534137415895 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 44010 0.27199527725718997 No Hit
ACCTCCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCC 43060 0.2661239863370734 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 42381 0.261927558405748 No Hit
CTGATCAGGAAAGACTACCCGCTGAGTTTAAGCATATCACTAAGCGGAGG 42055 0.25991277857421324 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 35042 0.21657029097128952 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 34302 0.21199686435983028 No Hit
TGGGTCGGGGGGAGCGGTCCGCCCCTTGTGGGTGTGCACTGGTCCACTCG 31650 0.19560669223335747 No Hit
GCAGACCTCTGATCAGGAAAGACTACCCGCTGAGTTTAAGCATATCACTA 31323 0.19358573209559105 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 30870 0.19078605337263022 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 30292 0.18721383637070663 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 26553 0.16410567137037413 No Hit
GAAACATCTTAGTAGCCAGAGGAAAAGAAAGCAAAAGCGATTCCCATAGT 25004 0.15453237701746828 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 25000 0.15450765579254147 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 24226 0.14972409876920437 No Hit
TGACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTC 23573 0.1456883587999032 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 23342 0.1442607080603801 No Hit
GCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATG 22410 0.13850066265243416 No Hit
AAGGCTAAATATTGGCAAGAGACCGATAGCGAACAAGTACCGCGAGGGAA 22075 0.1364302600648141 No Hit
CCACGAGGCGCGATCTGCGAGTCGGGCTGTTTGGGAATGCAGCCCTAAGT 21983 0.13586167189149756 No Hit
TGGCAAGAGACCGATAGCGAACAAGTACCGCGAGGGAAAGATGAAAAGGA 21828 0.1349037244255838 No Hit
AACACGGACCAAGGAGTCTAACATGTATGCAAGCAGGTGGGCGGCAAACC 21609 0.13355023736084112 No Hit
AGCAAGCGACGAAATGCTTCGGGGAGCTGAAAATAAGCATAGATCCGGAG 21383 0.13215348815247657 No Hit
ACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCA 21123 0.13054660853223413 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 20693 0.12788907685260242 No Hit
AAGTGGGAGGTAAATTCCTTCTAAGGCTAAATATTGGCAAGAGACCGATA 19994 0.12356904279664296 No Hit
CTAAGGCTAAATATTGGCAAGAGACCGATAGCGAACAAGTACCGCGAGGG 19960 0.12335891238476511 No Hit
CAACGCGAAGAACCTTACCAGGGCTTGACATGCCGCGAATCCTTTTGAAA 19721 0.1218818191953884 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 19407 0.11994120303863408 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 19394 0.11986085905762195 No Hit
TAGTAGCCAGAGGAAAAGAAAGCAAAAGCGATTCCCATAGTAGCGGCGAG 18962 0.11719096676552684 No Hit
ACCAATAGGGGTGCTTCGTGCACCCCCAGAAGCATGTGTCGAGAGCTCCC 18588 0.11487953223487044 No Hit
AAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCG 18551 0.11465086090429745 No Hit
ACAGACCTCTGATCAGGAAAGACTACCCGCTGAGTTTAAGCATATCACTA 18256 0.11282767056594548 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 17958 0.11098593930889838 No Hit
CCAGACCTCTGATCAGGAAAGACTACCCGCTGAGTTTAAGCATATCACTA 17934 0.11083761195933754 No Hit
GCTCCACGAGGCGCGATCTGCGAGTCGGGCTGTTTGGGAATGCAGCCCTA 17885 0.11053477695398416 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 17697 0.10937287938242424 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 17597 0.10875484875925408 No Hit
GTAGGTGGCTTTTTAAGTCTGCTGTCAAATCCCAGGGCTCAACCCTGGAC 17492 0.10810591660492541 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 17440 0.10778454068087692 No Hit
ACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGACGG 17170 0.10611585799831748 No Hit
GTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATATTGATGGTAT 16523 0.10211719986640651 No Hit
TGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGACTGAAGTGGAG 16334 0.1009491219886149 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 16299 0.10073281127050532 No Hit
GCAGCGTGCCGGCCCCAAGTTCCCTGGAATGGGATGCCCAAGAGGGTGAC 16297 0.10072045065804194 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 6475965 3.1754594 7.989424 70-74
CTGCT 4144970 3.1605117 7.55632 4
CTTCT 4311600 3.0482216 8.7402725 45-49
AGCAC 3434365 2.7502942 7.2034864 70-74
ACTTC 3672435 2.6607902 5.8404093 60-64
AACTT 4574495 2.660566 5.6580677 10-14
TCCTT 3548370 2.5086322 10.318643 45-49
GAAGA 4604640 2.5006726 5.2474103 90-94
TCTCT 3530710 2.496147 7.713048 20-24
TGAAA 4919640 2.4772313 5.8215675 65-69
CGGAA 3638370 2.461464 5.992694 95-97
CTGGT 3801045 2.4484596 12.163382 1
AAGAG 4458945 2.421549 5.414687 65-69
ATCGG 3632840 2.3981957 7.4005976 95-97
CTGAA 3792795 2.37913 5.8968005 80-84
AGAGC 3388570 2.2924676 7.064479 95-97
TGGTG 4208475 2.290175 7.3088584 4
AGCTG 3456330 2.2816737 5.487085 1
TCGGA 3387155 2.2360082 6.2862606 95-97
AGATC 3550210 2.226962 8.275547 95-97
GGTGG 3793830 2.226638 6.072228 7
GAAAC 3439785 2.211253 5.024654 60-64
CTACT 3008245 2.1795645 8.728412 2
TGCTA 3500695 2.1427164 5.211259 4
GATCG 3223795 2.1281672 7.830346 95-97
TTCAA 3656310 2.1265419 8.699757 9
ATGCA 3386845 2.1244872 5.6380253 20-24
CTTCA 2921300 2.11657 6.006388 60-64
GCAGC 2477005 2.0875604 7.8601356 20-24
TCAAC 2795170 2.0754535 6.6815286 8
CTCTG 2687080 2.0488803 8.552762 6
TATGC 3331180 2.0389593 5.799222 30-34
TTCCT 2882845 2.0381184 8.647916 45-49
ATTCC 2800685 2.029181 6.234059 40-44
CCTTA 2731550 1.9790903 6.3303924 95-97
GTCTG 3067010 1.9756277 5.75864 90-94
CGGTG 2824685 1.9624015 6.7828336 9
CACAC 2050270 1.9435235 7.4016643 95-97
GGTGT 3548060 1.9307892 6.8110695 1
CAAGC 2387975 1.9123285 5.2662997 30-34
TTTCA 3321895 1.8852478 9.520212 8
CTGTT 3133745 1.8716549 5.9062896 10-14
GAAAG 3439085 1.8676869 5.1915755 7
GAGCA 2748795 1.8596408 5.3532333 95-97
CATGG 2814890 1.8582313 6.391262 2
GCACC 1858420 1.8539674 6.880133 70-74
CAACT 2475380 1.838005 7.5870657 9
GCTGC 2228960 1.8330153 5.519357 3
AGGAA 3366005 1.8279988 5.570367 10-14
CAAGT 2913330 1.8274624 5.004906 85-89
GCACA 2275780 1.8224808 6.505498 95-97
GCAAG 2652015 1.794166 5.735242 65-69
CCCTA 1937445 1.7920897 7.457716 25-29
CAGCA 2207455 1.7677652 5.0020585 70-74
ACTTT 3109000 1.7644253 9.212412 6
GCTTC 2309415 1.7609131 6.165647 15-19
CTAAG 2789275 1.7496457 5.567972 50-54
CTTTC 2472470 1.7479907 10.700569 7
GAGAA 3213970 1.7454321 5.1366644 80-84
GCGAA 2556880 1.7298043 5.561908 80-84
GCTGG 2486670 1.7275714 6.6917815 2
TGGGA 3094365 1.7256941 6.044929 30-34
GCTTG 2670120 1.7199693 5.202805 65-69
CAGCC 1723900 1.7197698 7.842136 20-24
ACCCA 1811635 1.7173129 22.229786 1
TGCAG 2591190 1.710557 6.3621364 20-24
GGAGG 2836230 1.7059315 6.0237937 35-39
ACTCC 1842830 1.7045732 5.424497 60-64
CTACC 1842380 1.7041569 7.7869315 20-24
CCAGC 1681880 1.6778504 5.7581873 70-74
ATGCC 2139930 1.6721834 5.356101 30-34
TCTGA 2731320 1.6717949 6.9458694 7
CACCC 1410800 1.6659799 5.852339 50-54
TACTT 2931985 1.6639653 9.076191 5
GCGAG 2316715 1.6494486 25.44865 1
CCGCC 1325785 1.6476194 7.042932 65-69
TTGGT 3265350 1.6475772 13.833107 7
GGAAA 3001050 1.6298001 5.238977 9
TGTTT 3481660 1.6288253 5.2982783 10-14
CAACA 2138070 1.6269532 5.4888196 1
CCAAT 2188465 1.6249665 16.993969 3
ACACG 2022170 1.619386 5.73743 3
TTCGG 2432465 1.5668828 5.8995132 7
GAACA 2407125 1.5474113 8.523773 80-84
GCTAA 2451285 1.5376326 6.194111 55-59
GGGAA 2683115 1.5334864 5.8184237 15-19
CAAGA 2366540 1.5213214 5.308217 65-69
ACTGC 1944250 1.5192752 6.0948453 2
GGAAT 2840570 1.5052831 5.6182313 15-19
TTTGG 2965820 1.4964453 5.0934873 95-97
CTCTA 2058240 1.491257 5.435906 95-97
AGAGA 2723750 1.4792051 5.231209 70-74
ACCGC 1467950 1.4644331 6.7991953 90-94
GCTGT 2237105 1.4410409 5.9806604 10-14
GGGAG 2393765 1.4397982 5.349162 35-39
AGCCC 1428320 1.424898 8.48527 25-29
GCTAC 1813695 1.417257 8.730446 1
TTGGG 2559925 1.3930643 5.0463557 95-97
ATTGG 2680080 1.3858371 12.0364685 6
GTGTA 2657915 1.3743758 6.3635015 2
TGCCG 1670345 1.3736309 5.614252 30-34
AGCGA 2024535 1.3696575 5.5618424 80-84
CAACC 1435850 1.361093 5.779349 15-19
TGGTA 2628935 1.3593906 8.815226 2
CATCC 1463400 1.3536096 5.118031 90-94
CGCGA 1600440 1.3488126 5.4831543 90-94
TCGGT 2088615 1.3453904 6.406159 8
GCCCT 1375840 1.3392999 8.153428 25-29
CGAGG 1874525 1.3346193 6.428349 95-97
CAGGA 1959610 1.3257338 5.396798 4
ATCTC 1823420 1.3211229 5.8602724 45-49
TACCC 1423630 1.3168232 5.484869 20-24
GAGTC 1991625 1.3147583 23.192736 3
CCCAA 1384020 1.3119615 22.306341 2
GTTAC 2139400 1.3094907 5.9572067 9
GGCTG 1851445 1.2862597 26.239265 9
AGGGA 2234295 1.2769715 5.1630597 95-97
CCAGT 1626235 1.270772 6.069457 3
TGGTC 1957290 1.2607969 15.087849 8
CGCCA 1256765 1.253754 5.3526096 65-69
AACAC 1645600 1.2522107 7.6800838 2
ACGGT 1869640 1.2342305 5.203984 4
CCTTC 1355135 1.2231073 7.1655416 45-49
GGCAA 1789660 1.2107576 5.337717 65-69
CCACA 1264900 1.1990435 5.058998 60-64
CCTCC 1038865 1.1970576 6.530604 2
GGGCT 1718180 1.1936761 25.04602 8
ACAAG 1842570 1.1844893 5.3290596 85-89
TACCG 1514055 1.1831124 19.864231 3
AGACC 1467640 1.1753095 9.185325 2
ACATG 1861215 1.1674957 5.334911 1
CGAAC 1442150 1.1548966 5.7560253 80-84
GCCAG 1369545 1.1542197 6.5039334 2
AAGGC 1702295 1.1516527 5.482022 50-54
AGGTA 2171530 1.1507438 5.549182 35-39
CCTAG 1461745 1.1422364 18.741766 9
CCGAC 1142545 1.1398077 5.3463597 30-34
CCGCG 1082355 1.1363379 6.840842 90-94
GAGGG 1859755 1.118603 5.418711 95-97
AATGC 1759815 1.10389 5.2241025 20-24
CACCT 1175165 1.0869992 6.0294642 70-74
AAGAC 1681195 1.0807499 5.3248715 9
TCGGG 1538370 1.0687562 24.935177 6
GACCG 1267680 1.0683705 5.668934 70-74
CCCCG 839770 1.0436242 11.401297 1
GTCCT 1354845 1.0330602 18.727453 7
ACCGA 1276435 1.0221895 5.471135 70-74
AGGCT 1545285 1.0201098 5.1775284 50-54
CCTAA 1368315 1.0159934 5.7603297 25-29
GTACT 1650305 1.010124 9.021908 4
GAGGT 1798290 1.0028871 5.1059794 35-39
ACCGT 1278895 0.99935377 19.160965 4
TGGCA 1512625 0.99854946 5.1192393 65-69
CCGTC 1010580 0.98374057 23.50082 5
GGTAC 1482105 0.97840184 9.650594 3
TGATC 1597260 0.977656 7.656426 9
TCCTA 1331490 0.96470463 17.731457 8
CAATT 1634900 0.95087206 13.388024 4
ATTCG 1550105 0.94879323 5.01537 6
CCGAT 1208725 0.94452155 5.638726 75-79
CTCCC 813625 0.93751925 6.102606 3
GGCTA 1411040 0.93148875 5.337939 55-59
GGTCA 1396945 0.92218405 15.220032 9
ACCTC 994220 0.91962945 10.544396 4
GTACC 1160645 0.9069509 5.195023 85-89
CCCGC 729570 0.90667313 5.5015516 25-29
CGAGT 1364710 0.9009043 23.235624 2
CTGAC 1150450 0.8989843 6.120151 1
TCCCC 777250 0.89560527 5.747803 4
CAGAC 1110595 0.8893822 9.532597 1
CGGGC 992445 0.88023406 31.556246 7
CGGGG 1170975 0.87739134 7.707349 6
CGGTA 1320365 0.87163025 5.5618634 5
AATTG 1765705 0.867567 10.706228 5
CACGG 1028170 0.8665171 7.724213 4
CCTCT 959750 0.8662438 10.447877 5
GTCGG 1245735 0.86545306 24.933826 5
TGGCC 1037750 0.8534078 5.8682203 1
CGTCC 871260 0.8481207 23.26056 6
TCCGC 860565 0.8377097 5.1298685 65-69
AGTCG 1263615 0.8341672 23.427692 4
GCAAC 1035920 0.8295812 5.141171 1
TGCCA 1052370 0.8223426 6.3459024 1
CGGTT 1253450 0.80741525 6.108709 4
TAGCG 1211195 0.7995625 5.0510554 75-79
CCGGT 957440 0.78736377 8.091416 3
GGTTG 1436965 0.78197 6.774508 5
CTCCG 775120 0.754534 5.116178 65-69
CTGAT 1189610 0.7281403 6.8692946 8
ATACC 963305 0.7152677 18.105803 2
CCCGG 637265 0.66904885 9.742974 2
GACCT 827575 0.6466834 8.753311 3
GATAC 960905 0.6027528 15.182488 1
Produced by FastQC (version 0.10.1)