FastQCFastQC Report
Mon 21 Jul 2014
3L15B_GCCAAT_L003_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 3L15B_GCCAAT_L003_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 16257179
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 267945 1.6481641741165551 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 146006 0.8981016940269896 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 135548 0.8337731902933467 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 135509 0.8335332962748333 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 117272 0.7213551625408073 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 97557 0.6000856606179953 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 97369 0.5989292484261876 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 91131 0.5605585077214196 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 83684 0.5147510524427393 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 77076 0.4741043941264349 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 69566 0.4279094177409254 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 63541 0.3908488674449608 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 58011 0.3568331258455111 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 57713 0.3550000894989223 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 55020 0.33843509996414506 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 53639 0.3299403912573024 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 50602 0.3112594134566643 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 49445 0.30414255757410313 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 48941 0.30104238871946976 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 44847 0.27585966790425326 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 44575 0.27418656090334004 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 42114 0.2590486332222829 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 40310 0.24795199708387292 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 40058 0.24640191265655623 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 39421 0.24248364368750572 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 39307 0.24178241501800526 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 37098 0.2281945717642649 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 35832 0.22040724285560245 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 35610 0.2190416922886806 No Hit
GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA 33833 0.20811113662462596 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 32210 0.19812785477726488 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 30954 0.1904020371554007 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 29770 0.18311910079848415 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 29621 0.18220258262518976 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 28801 0.17715865710773068 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 26837 0.16507784038054818 No Hit
GGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTA 26536 0.16322635064791993 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 26306 0.16181159105155943 No Hit
ATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGATTTCACTAAA 25916 0.15941265086642645 No Hit
GTATGGGCTTGGATGTTCTTATTTGGACATCTTGTTTGGGCTACTGGGTT 25274 0.15546362625397678 No Hit
TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC 24475 0.15054887443879408 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 24409 0.15014289994592542 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 24392 0.15003833075836834 No Hit
GGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTATTT 23895 0.14698121980449375 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 23646 0.14544958876321656 No Hit
CCACGTAGTTGGTTAGCTACTTCTCACTTTGTATTAGGATTTTTCTTCTT 23415 0.14402867803817626 No Hit
GCGACTGGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGT 22989 0.14140829722056944 No Hit
GGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCAT 22974 0.141316030290372 No Hit
GTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAG 22074 0.1357800144785267 No Hit
TTCATGCGTTGGAGAGACCGTTTCTTATTCTGTGCAGAAGCTATTTACAA 21342 0.13127738828489247 No Hit
CGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTC 21335 0.13123433038413368 No Hit
GCTCATGGTAAGGCTTTATATGGTTTTGATGTTCTTTTATCATCTGCAGA 21234 0.13061306638747103 No Hit
ATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCATCTC 21192 0.13035471898291826 No Hit
ACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGACTG 21148 0.1300840693210058 No Hit
GTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCTTTCAA 20778 0.1278081517094694 No Hit
TACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAAC 20413 0.12556298974133212 No Hit
TACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGA 20155 0.12397599854193646 No Hit
CAAGGTGAAACAGGTGAAATTAAAGGGCATTATCTAAATGCTACTGCAGG 20128 0.1238099180675811 No Hit
CTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGT 19330 0.11890131738107822 No Hit
CATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGC 19152 0.1178064164760688 No Hit
GGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCTGGTACTTT 19017 0.11697601410429201 No Hit
GGTCCTTACGAGTTAATCGTTCTTCACTTCTTACTTGGTGTAGCTTGCTA 18985 0.11677917798653753 No Hit
ACCCAATTTGGGAAGCTGCTTCCGTTGATGAATGGCTTTACAACGGTGGT 18917 0.1163609012363092 No Hit
GTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCATCTCTGCT 18710 0.11508761759958477 No Hit
GACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCT 18396 0.11315616319411874 No Hit
CCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCCAGAA 17908 0.1101544123983626 No Hit
GTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGG 17425 0.10718341724600561 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
ACTTC 5458330 3.5149527 6.532881 60-64
CTGCT 5134620 3.49809 12.7391815 5
AAAAA 5592270 3.4769757 6.211139 45-49
AACTT 6066290 3.1884513 5.424517 60-64
CTACT 4613320 2.970799 13.73591 2
CAACT 3763930 2.9179733 7.530215 55-59
AGCTG 4071520 2.8834941 8.284895 3
GGAAG 3863570 2.844403 5.745914 15-19
TCAAG 4196670 2.8093405 5.1087737 10-14
TTCAA 5253315 2.76115 7.2942734 55-59
CTTCA 4255615 2.7404509 6.3922195 60-64
CTTCT 5032470 2.691912 7.003055 40-44
TCAAC 3444170 2.6700804 7.1742363 55-59
CTGGT 4475630 2.6329136 11.416709 1
TCTCT 4920685 2.632117 8.363138 20-24
GCTGC 3020075 2.6204958 8.44639 3
AGGAA 3753745 2.6121864 6.2954593 40-44
AGCAC 2627725 2.5945542 5.729708 35-39
ACCCA 2255030 2.578549 35.78745 1
ACTGC 3123735 2.561987 13.362765 4
ACAAC 2624280 2.4492328 6.7865915 85-89
GAAGC 2846315 2.426753 6.6311 15-19
CAAGC 2416055 2.385556 7.262876 75-79
CGGTG 3160970 2.3683498 13.360355 9
AAGCT 3530020 2.363071 5.101474 15-19
TATGC 4246060 2.3610525 6.851741 30-34
CAGCA 2389970 2.3598003 6.1735854 35-39
GCAGC 2249065 2.3493502 7.936544 1
GGTTC 3976735 2.339425 6.812554 3
GGTGT 4553320 2.3129723 10.454844 1
CTGAA 3451410 2.3104475 5.61604 80-84
GGTGG 3566280 2.3072822 9.664731 7
CAAGG 2704215 2.3055992 6.199149 10-14
TGGTG 4525230 2.2987034 8.286874 6
CTGCA 2790395 2.288592 6.514736 95-97
CAACA 2444935 2.2818506 6.637579 85-89
GCTAC 2756140 2.2604976 17.868137 1
ACATC 2886400 2.2376714 5.4704742 90-94
CCAAG 2195345 2.167632 6.9033437 75-79
CATGG 3059080 2.1664734 13.155615 2
ATGGT 4497445 2.1594598 8.048268 1
CTCTG 3164915 2.1561785 7.747948 45-49
TCTGC 3146410 2.1435716 5.1393476 85-89
CAGCT 2607200 2.1383417 6.5397406 65-69
ATGCA 3152595 2.1104143 5.6869555 1
CCGTG 2412530 2.0933335 5.686019 60-64
TGCAG 2921755 2.0692186 5.5253625 65-69
TCCTT 3867600 2.0688128 6.26608 40-44
CGTGA 2899810 2.0536766 11.167222 9
GCTCC 2019525 2.0293417 7.237101 70-74
CTCCT 2566180 2.0246503 8.637807 70-74
CGGAA 2332965 1.9890735 5.371697 95-97
CTTGG 3373260 1.9844137 7.032506 2
GCTGG 2646005 1.9825135 9.617367 4
TTTCA 4538565 1.9815043 5.9429526 8
CCAGC 1616215 1.9551725 6.7522297 1
AAGGA 2785705 1.9385391 5.5392256 10-14
AGCTC 2349400 1.9269023 5.890372 70-74
GGTAT 4002070 1.9216044 5.4707546 6
GCTTC 2814155 1.9172146 7.7134123 15-19
CCCAA 1662475 1.9009829 36.301014 2
TGCTG 3229485 1.8998345 9.86107 6
CCAAT 2446830 1.8968961 24.767744 3
TAGCT 3378715 1.878759 5.950534 2
TTCCA 2906970 1.8719759 5.239139 75-79
CCTTA 2880695 1.8550558 6.7450643 95-97
CACAA 1979930 1.8478626 6.3580213 85-89
CTGTT 3994625 1.845081 7.9616776 8
TGGCT 3118740 1.8346857 7.0337405 9
CTCTA 2826675 1.820269 5.818429 25-29
CTTTC 3363535 1.7991841 7.015328 7
CATCC 1882265 1.7878197 6.458823 90-94
TACTG 3194690 1.7764306 9.2074585 3
GAAAA 2699510 1.7756665 5.137351 40-44
CCTGT 2594355 1.7674701 8.5261345 1
TATTC 4047695 1.767194 6.4956846 5
ATCCG 2154020 1.7666581 5.6728635 95-97
GCAGG 1958420 1.7664909 5.3135824 90-94
GGCTC 2020500 1.7531722 5.209663 10-14
CACCC 1245590 1.7450235 6.142633 50-54
AATGA 3175845 1.7352245 6.1774683 75-79
TTCGG 2945915 1.7330163 10.342173 7
TCGGT 2868170 1.6872805 12.298988 8
GCACC 1384125 1.6744081 5.499265 50-54
ACCAG 1691980 1.6706214 5.666112 30-34
TGGTA 3458490 1.660603 9.017503 2
CCTAC 1745005 1.657447 5.2922134 80-84
CCACA 1446220 1.6537026 5.57544 1
GTGGC 2193530 1.6434978 8.301079 8
TTGGT 4110825 1.6395633 13.850267 7
CCGCC 1103100 1.6349485 8.12523 45-49
ACGGT 2301150 1.6296992 8.234407 4
GCAAC 1645395 1.6246246 5.7821283 1
GCTGT 2745390 1.6150521 10.022655 7
CTACC 1698945 1.6136981 6.043965 20-24
CCTCC 1384115 1.6107136 5.1553035 1
TGTAG 3341480 1.6044203 6.1016283 9
ATGCC 1942610 1.5932662 8.687881 9
TACTT 3647455 1.5924522 6.4743004 5
CTGTA 2856080 1.588144 6.801123 8
GAACA 1954535 1.5751543 5.6837564 80-84
TGAAC 2319870 1.5529708 5.2734675 80-84
GCTTG 2603230 1.5314225 6.840826 1
ATTCC 2372835 1.5280137 5.348854 80-84
GGTAC 2143245 1.5178694 10.21814 3
ACACA 1617505 1.5096127 6.036278 85-89
CATGC 1840160 1.50924 5.1359763 2
GCACT 1838475 1.5078582 5.5810614 35-39
CGCAG 1439085 1.5032535 6.0938873 90-94
TCCAA 1927710 1.4944503 5.355979 75-79
GTAGC 2098285 1.4860282 7.075931 1
CACAC 1293725 1.4793297 5.4044275 95-97
GTGTA 3078785 1.4782867 9.670641 2
GACGG 1638000 1.4774727 10.653803 3
TTCAT 3382360 1.4767137 5.181413 60-64
ATCTC 2254190 1.4516109 5.1813354 45-49
AACAC 1545775 1.4426672 6.0973854 85-89
ACATG 2144445 1.4355372 12.516114 1
GGCTG 1902515 1.4254552 6.4272866 9
GTGTT 3545905 1.4142504 5.0540266 9
ACTTT 3226955 1.408865 6.327778 6
GCGGC 1271925 1.4056275 8.171824 7
GGTCA 1961630 1.3892474 20.404264 9
AACCA 1477035 1.3785124 5.032045 75-79
TGTTT 4341905 1.3596792 5.8403883 9
AGCCT 1650435 1.3536338 7.5796723 2
ATGGG 2208340 1.3504802 11.948777 3
AGGTA 2319500 1.3407682 5.871552 6
GTATT 3555310 1.3403362 6.0144916 4
TGGTC 2260790 1.3299726 17.151428 8
CAATT 2525780 1.3275535 16.82848 4
GTCTG 2227720 1.3105184 5.170894 90-94
GGTCG 1746060 1.3082317 11.428257 6
CTGAC 1579565 1.2955084 9.328636 1
ACCGC 1070795 1.2953655 5.328379 45-49
GTATG 2678645 1.2861584 5.7853975 7
GTACT 2309185 1.2840391 7.87481 4
AAGCC 1274425 1.2583375 8.34493 1
TGACG 1747925 1.2378994 8.060969 2
GCCTG 1417980 1.2303703 8.015495 6
TGGGT 2359695 1.1986659 8.330488 4
TGTAT 3171260 1.195551 5.90626 3
GGGTC 1542095 1.1554115 11.401025 5
ATTGG 2390935 1.1480136 14.515718 6
TGGCC 1317450 1.1431412 9.937876 1
GCGAC 1094185 1.1429744 5.8825502 20-24
ATTCG 1976580 1.0990916 8.078659 6
CGCCT 1080040 1.08529 5.2551455 45-49
CGGTA 1532035 1.0850036 7.6618876 5
TCGTG 1813095 1.0666034 8.902938 8
TGCGG 1402185 1.0505841 5.8316703 6
GTAGA 1806680 1.0443369 5.0762963 4
TTGGC 1750740 1.0299214 5.5724287 3
TGGGG 1583495 1.0244764 6.5743237 8
AATTG 2247665 1.0201122 13.259381 5
GTTCG 1726710 1.0157851 6.396896 4
CGGCT 1153395 1.0007918 6.188967 8
GGGGT 1421585 0.91972524 6.1498194 9
GCCCA 733450 0.88727146 5.5602694 2
GGCCT 935705 0.8119039 7.767666 5
GCCTA 988795 0.8109779 7.2694373 3
GGCCC 626645 0.80199283 6.86252 1
CTACA 1005330 0.7793785 5.1821284 3
GTCGT 1284855 0.75585175 8.75884 7
GCCCT 716850 0.72033453 6.094382 1
CCCTA 755850 0.7179242 5.182356 2
CCTAT 1058165 0.6814172 5.486557 4
GGTAA 1042380 0.6025394 5.4309535 7
Produced by FastQC (version 0.10.1)