FastQCFastQC Report
Mon 21 Jul 2014
3L15A_GCCAAT_L002_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 3L15A_GCCAAT_L002_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 12287857
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 145357 1.1829320604886597 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 119377 0.9715038187700263 No Hit
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 104577 0.8510597087840459 No Hit
ATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCA 90523 0.7366866329906021 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 86455 0.7035807789755366 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 80911 0.6584630664240315 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 74158 0.603506372185158 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 73746 0.6001534685828457 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 72019 0.5860989430459681 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 56362 0.45868046804255613 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 56076 0.4563529669982325 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 52365 0.42615242023080185 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 47948 0.39020636389241836 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 46743 0.38039993466720845 No Hit
TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC 45074 0.3668174198316273 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 43348 0.3527710324102893 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 42989 0.34984944893157527 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 37953 0.30886589907418355 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 32201 0.26205545849044304 No Hit
GTATGGGCTTGGATGTTCTTATTTGGACATCTTGTTTGGGCTACTGGGTT 30059 0.24462361500463425 No Hit
CATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGC 28671 0.23332791063567876 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 28418 0.2312689674041617 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 28355 0.2307562661251673 No Hit
GCTAAAAACTATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGA 27874 0.22684183255062296 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 27615 0.22473406062586831 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 26579 0.21630297292684966 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 26195 0.21317793655964584 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 25475 0.2073184933711387 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 25163 0.20477940132278558 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 23041 0.18751032014776864 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 23034 0.1874533533389915 No Hit
CCACGTAGTTGGTTAGCTACTTCTCACTTTGTATTAGGATTTTTCTTCTT 22957 0.1868267184424428 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 22486 0.18299366602329437 No Hit
CACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTAT 22045 0.17940475707033374 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 20929 0.17032262012814764 No Hit
GCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATG 20819 0.16942742741879238 No Hit
AGCTCCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTC 19953 0.1623798193615046 No Hit
CTGCTGCTATCATCCCTACTTCTGCAGCTATCGGTTTGCACTTCTACCCA 19926 0.1621600902419356 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 19581 0.15935244038077592 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 19481 0.1585386288268166 No Hit
TTCATGCGTTGGAGAGACCGTTTCTTATTCTGTGCAGAAGCTATTTACAA 18676 0.151987445817444 No Hit
GCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGT 17418 0.14174969646863567 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 17008 0.13841306909740242 No Hit
TATGCACCCATTCCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTC 16495 0.1342382158255911 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 14592 0.11875138195374507 No Hit
ATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAA 14571 0.11858048152741361 No Hit
TACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTAT 14423 0.1173760404275538 No Hit
GATGAAATTAGCAGTATTATCCGTAAACAGATAGAAGACTATAGTCAAGA 14183 0.11542289269805142 No Hit
ATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGATTTCACTAAA 14155 0.11519502546294282 No Hit
CTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGT 13482 0.10971807370479653 No Hit
GTAGGTGGTGTAGCTACTGAAATTAATGCAGTAAATTATGTGTCTCCACG 13437 0.10935185850551483 No Hit
GCTCATGGTAAGGCTTTATATGGTTTTGATGTTCTTTTATCATCTGCAGA 13267 0.10796837886378398 No Hit
GTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCC 13156 0.10706504803888912 No Hit
GGTGGTGACCACATTCACTCCGGTACTGTAGTAGGTAAACTTGAAGGGGA 13063 0.10630820329370694 No Hit
TGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCT 12811 0.10425739817772944 No Hit
CTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCC 12792 0.10410277398247716 No Hit
GTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAG 12531 0.10197872582664333 No Hit
TTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGT 12476 0.1015311294719657 No Hit
GGCTGGGCAGGTTCTATGGCTTTATACGAATTAGCTGTTTTTGATCCTTC 12333 0.10036737894980387 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 12145155 8.536861 13.113105 95-97
ACTTC 3964400 3.7857502 8.770779 65-69
CTGCT 3544090 3.5565522 11.371141 4
CTACT 3388795 3.236084 10.019522 85-89
AGCTG 3489585 3.1572468 10.827793 3
CTTCA 3305275 3.156328 9.679456 65-69
AACTT 4322230 3.1194715 6.1625986 60-64
TGCTA 4092605 3.1040084 5.505481 40-44
TCAAG 3492860 3.0072253 6.5977283 65-69
TCTCT 3514300 2.9563215 8.38501 20-24
CTCTC 2337235 2.953101 11.718226 30-34
TTCAA 4005460 2.8908498 6.9429107 65-69
CCGTG 2382445 2.8520625 7.868334 80-84
TGGTG 4459985 2.8232868 15.058526 6
CTGGT 3467120 2.7633882 9.221516 5
GAAGA 3488485 2.707896 5.271824 95-97
GGTGG 3476585 2.6253433 8.780971 7
TATTC 4085835 2.5977194 9.9936905 5
TCCTT 3075765 2.5874143 7.970958 55-59
GGCTC 2149885 2.573661 8.049426 30-34
GCTGC 2148180 2.57162 12.981432 3
GAAAC 2630995 2.5713816 7.059165 80-84
GGTGT 4027675 2.549623 18.730108 9
GGTTC 3195715 2.5470712 7.2421646 4
GCTGG 2665800 2.5346165 12.002475 4
ATGCA 2935215 2.5271134 7.3802376 45-49
ATCCG 2212765 2.520702 8.204437 75-79
GTTCC 2501860 2.5106578 6.716014 55-59
CGGTG 2628240 2.4989047 15.872391 9
CATGG 2756415 2.4939017 8.086457 2
CGTGA 2730275 2.470251 6.982952 80-84
GAGAA 3169700 2.4604428 6.523195 90-94
TATGC 3191510 2.420579 7.728425 45-49
CAAGT 2789250 2.4014428 6.98974 70-74
TCCGT 2390380 2.3987856 6.633793 75-79
CTTCT 2849525 2.3970952 5.630937 40-44
CCTTA 2500555 2.3878715 7.0761824 55-59
TGGCT 2987860 2.381405 7.837033 9
GCTCT 2320230 2.3283887 7.749795 30-34
AGCAC 1779535 2.3012004 5.280666 90-94
CAACT 2116480 2.294301 5.773165 9
CTCTA 2392670 2.2848482 7.0291595 25-29
TCTGC 2272175 2.2801647 7.707248 85-89
TTCCT 2704015 2.2746885 6.6123176 55-59
TGAAA 3454445 2.2478209 5.589104 80-84
GTGGC 2362785 2.246513 8.849144 8
ATGGT 3642555 2.194208 6.9081907 2
AATCC 2020160 2.1898885 9.709334 75-79
ACTAC 2016150 2.1855416 9.721356 85-89
TGGTT 4110700 2.1813517 5.6416373 3
TCAAC 2005210 2.1736825 5.3505077 8
GCATG 2401280 2.1725888 6.4074016 50-54
AAACT 2631540 2.1559803 5.83087 85-89
CTTGG 2688425 2.1427472 14.528004 6
TACTG 2821295 2.139792 6.246161 85-89
ACCCA 1305505 2.125582 23.397123 1
GCAGC 1558030 2.1172574 5.5205317 1
GTGCT 2630640 2.0966911 6.2823443 40-44
ACTGA 2430010 2.0921502 5.8183923 90-94
CAGTG 2294975 2.0764081 6.9242554 40-44
GTGAA 3036440 2.076338 6.7682595 80-84
TGCAT 2737610 2.0763216 5.4157457 45-49
TAGCT 2733435 2.0731552 8.444508 2
CTGAG 2261975 2.0465508 6.8260384 90-94
CTATG 2646265 2.0070417 6.62141 45-49
AGTGC 2203640 1.9937716 8.33375 40-44
CTCCT 1567655 1.9807352 6.6243377 70-74
GTGTA 3284560 1.9785582 12.970386 2
CGGAA 1915890 1.9677368 5.7064357 95-97
TTCGG 2465540 1.9651018 13.213493 7
GCTAT 2584845 1.960458 5.2165303 45-49
TTCAG 2559940 1.9415691 5.669812 35-39
CTATT 3036545 1.9305946 5.385933 35-39
TCGGT 2398100 1.9113503 13.877917 8
TAACT 2619325 1.8904384 5.3080177 60-64
ATTCA 2590830 1.8698728 5.660081 35-39
GTAAC 2151415 1.8522902 6.4760113 60-64
CAGCA 1415165 1.8300164 5.3336005 2
GCTTG 2284495 1.820804 13.58413 5
AAGTT 3159775 1.8112476 5.3314824 70-74
GAATG 2647930 1.8106722 7.089883 95-97
GCTCC 1197700 1.8052428 5.7251067 70-74
TGCTT 2669615 1.7836512 11.448845 4
GCAGG 1634200 1.7638092 5.9982085 90-94
GCACA 1358970 1.7573481 5.690325 95-97
GATCG 1938425 1.7538149 5.6764402 95-97
AATGA 2678380 1.7428323 5.89911 95-97
GTAGC 1923850 1.740628 9.105055 1
CATGC 1527445 1.74001 17.685787 2
TGAGA 2541695 1.738028 6.5285068 90-94
CACAC 1065670 1.7350901 6.986323 95-97
CTTAG 2286005 1.733805 5.053944 55-59
AACTA 2098670 1.7194083 6.1029944 85-89
CCACA 1052135 1.7130531 15.534812 1
TTGGT 3195135 1.6955051 14.721357 7
TCGGA 1870850 1.6926755 5.000311 95-97
TGTAG 2805885 1.6902132 10.074497 9
CGCAG 1240165 1.6853002 7.6402645 90-94
CACCC 779705 1.6796973 5.21624 5
AGATC 1946345 1.675732 5.6222825 95-97
GCTAC 1463645 1.6673315 6.955725 1
CCTCC 875505 1.6614898 5.4951444 1
GTCTG 2071640 1.6511528 6.9853277 90-94
ATCGG 1810920 1.638453 5.150399 95-97
ATTCG 2150815 1.631271 11.820905 6
CTTTC 1931320 1.6246768 5.3641615 7
TCAGT 2081900 1.5790029 5.1241136 40-44
CCTGT 1572550 1.5780798 8.640844 1
AGTTT 3118215 1.5745848 5.4569545 70-74
GTATT 3066500 1.5484706 8.388647 4
CCCAA 946335 1.5407928 22.105946 2
ATGCT 2027955 1.5380887 12.316707 3
CCAAT 1415995 1.5349631 14.870779 3
GATGG 2132900 1.5326881 6.9446154 1
AGTAA 2346040 1.5265774 6.046919 60-64
GCACC 889045 1.5211536 5.0066805 4
CCAGC 887240 1.5180652 6.2010794 1
CACAA 1219295 1.500399 5.086706 4
AACGC 1152935 1.4909145 7.8156233 95-97
ATGAG 2143030 1.4654183 6.9477024 95-97
TAATC 2016100 1.4550744 5.2738247 75-79
TGTAT 2821775 1.4248934 8.115359 3
ACGCA 1094580 1.4154528 5.7850323 90-94
CTGTA 1844990 1.3993201 5.895202 8
ACATG 1554610 1.3384626 16.283432 1
CAGGT 1472360 1.3321366 6.4747167 95-97
TGGTA 2206760 1.3293115 5.572007 2
GGCTG 1386930 1.3186793 5.7755513 9
GCAAC 1015535 1.3132361 5.7206345 1
ATGGC 1436575 1.2997596 5.406689 1
CTAAC 1192805 1.2930213 6.0353312 95-97
ATGCC 1122340 1.2785292 6.045868 9
ACGGT 1354925 1.2258857 5.3823056 4
GCCTG 1019695 1.2206929 6.48404 6
ACACG 942085 1.2182544 5.3610234 95-97
CACAT 1113975 1.2075682 10.345693 2
TGGCC 995460 1.1916809 8.701714 1
GCGGC 796275 1.1371319 7.381078 7
GGTAC 1250805 1.1316819 6.1637425 3
TGGTC 1381610 1.1011806 9.554718 8
GACGG 1015740 1.0962988 6.5625095 3
GGTCA 1209835 1.0946138 10.598347 9
AAGCC 822895 1.0641242 6.8864956 1
GTACT 1384085 1.0497499 5.1404514 4
CAATT 1450460 1.0468366 10.143358 4
CTGAC 918310 1.0461055 6.524105 1
TGACG 1132490 1.0246348 5.5104628 2
ATGGG 1378030 0.99024343 7.449817 3
TGCGG 1032115 0.9813248 5.237913 6
ATTGG 1606875 0.9679518 7.923565 6
GCCCA 562395 0.9622562 5.607454 2
TAACG 1115905 0.96075356 5.207851 90-94
AAGGC 931985 0.957206 5.842671 1
TGGGC 984150 0.9357202 5.420964 4
GGTCG 910685 0.8658704 5.514564 6
AATTG 1493895 0.85633117 7.043512 5
GGCCC 470160 0.8453663 6.2728596 1
GGCCT 703615 0.8423085 6.2477603 5
AGGCC 615525 0.83645684 6.023409 2
CGGCT 690100 0.8261295 5.890163 8
CGGTA 871900 0.78886265 5.427937 5
GTTCG 979475 0.7806681 7.0040703 5
GGGTC 763735 0.72615165 5.4458613 5
CCACG 405715 0.69417727 5.3391953 1
Produced by FastQC (version 0.10.1)