FastQCFastQC Report
Mon 21 Jul 2014
20S15C_CTTGTA_L004_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 20S15C_CTTGTA_L004_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 12211451
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[WARN] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 183110 1.4994942042514032 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 99309 0.81324487974443 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 83212 0.6814259828745987 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 68470 0.5607032284697371 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 63383 0.5190456072746801 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 60761 0.49757395742733607 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 58523 0.4792468970313192 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 56820 0.4653009703760839 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 51513 0.4218417614745373 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 49797 0.40778937736391846 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 38781 0.3175789674789671 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 36896 0.3021426364483631 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 36637 0.3000216763757231 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 33588 0.27505330857078325 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 32744 0.2681417630058869 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 32726 0.2679943603753559 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 31103 0.2547035565224804 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 27818 0.22780257645057905 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 26361 0.21587115241260027 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 25612 0.20973756517550618 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 25533 0.2090906314081758 No Hit
GACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCT 25351 0.20760022703280714 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 25075 0.20534005336466568 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 24387 0.19970599726437097 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 23965 0.19625022448192275 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 23612 0.1933594951165099 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 22464 0.18395848290264605 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 21780 0.17835718294246933 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 21733 0.1779722982960829 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 21052 0.17239556544099469 No Hit
GCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATG 20635 0.16898073783369397 No Hit
ACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGACTG 19697 0.16129942297602473 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 19492 0.1596206707949776 No Hit
ATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTG 19329 0.1582858580851694 No Hit
GGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCAT 19014 0.15570631205087748 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 19008 0.1556571778407005 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 17901 0.14659191606304608 No Hit
CCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCCAGAA 17871 0.14634624501216112 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 17738 0.14525710335323788 No Hit
ATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAA 17279 0.14149833627469824 No Hit
CCACGTAGTTGGTTAGCTACTTCTCACTTTGTATTAGGATTTTTCTTCTT 16344 0.13384158852211747 No Hit
GTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATATTGATGGTAT 16119 0.1319990556404804 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 16045 0.13139306704829753 No Hit
TGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 15783 0.12924753987056903 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 15043 0.1231876539487404 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 14955 0.12246701886614458 No Hit
GTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGG 14866 0.12173819474851923 No Hit
TTCATGCGTTGGAGAGACCGTTTCTTATTCTGTGCAGAAGCTATTTACAA 14671 0.12014133291776709 No Hit
TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC 14408 0.11798761670500908 No Hit
ACTCTATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCC 14119 0.11562098558148413 No Hit
CATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGC 13937 0.11413058120611547 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 13858 0.11348364743878513 No Hit
CTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGT 13851 0.11342632419357862 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 13088 0.10717809046607155 No Hit
TGACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTC 12977 0.10626910757779726 No Hit
TACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGA 12655 0.10363223829829887 No Hit
CCTACTCTATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGC 12643 0.10353396987794489 No Hit
TACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAAC 12611 0.10327192075700095 No Hit
TGACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 12450 0.10195348611725175 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 12439 0.10186340673192729 No Hit
GCGACTGGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGT 12237 0.10020922165596864 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGCT 3747660 3.4121647 7.102334 5
AAAAA 4273925 3.3769503 4.6622167 70-74
ACTTC 3957935 3.3381705 6.9393578 60-64
GGAAG 3161285 3.1423273 5.987529 15-19
AACTT 4497385 3.094837 6.5269246 7
AGCTG 3196085 3.0405147 7.4076815 7
AGCAC 2364840 3.0300786 6.73196 80-84
GAAGA 3232310 2.9762595 5.8539624 90-94
CTGGT 3444290 2.762087 12.844238 1
CTACT 3265430 2.7541034 9.814975 2
TCAAG 3118315 2.7480128 5.2360525 10-14
TCTCT 3851490 2.7382774 8.250918 20-24
CTTCA 3165340 2.6696858 7.2735105 60-64
CTTCT 3693340 2.6258383 5.524653 20-24
CGGAA 2298745 2.5942435 7.156334 95-97
TTCAA 3746965 2.5784419 8.078571 9
CAACT 2535270 2.5366187 9.64419 9
GCTGC 2163775 2.5229177 7.1906147 3
CTGAA 2858540 2.5190861 6.328597 80-84
TGGTG 3550420 2.5077558 7.873068 6
ACAAC 2105730 2.499337 7.2321963 85-89
AGGAA 2665935 2.4547503 6.069616 40-44
GGTGT 3468900 2.450176 9.712043 1
CAAGC 1895400 2.4285831 7.3652287 75-79
CAACA 2025555 2.4041755 6.971527 85-89
CGGTG 2340175 2.4032953 10.972718 9
GGTGG 2651520 2.3983998 9.37814 7
GCAGC 1733815 2.3981972 5.6658616 1
AGAGC 2109660 2.380852 8.727494 95-97
ATGCA 2659065 2.3432994 6.1660876 1
CCGTG 2005935 2.3388793 5.9405894 60-64
TATGC 3146465 2.3373868 6.953369 30-34
ACCCA 1584900 2.3056118 32.7107 1
GAAGC 2007065 2.2650688 6.798198 15-19
ACATC 2261685 2.2628884 5.729675 90-94
TCTGC 2478180 2.2563303 5.629725 85-89
AAGCT 2559440 2.2555048 5.256804 15-19
AAGAG 2448670 2.2546961 5.1330137 90-94
TCAAC 2238675 2.2398658 7.771921 8
CCAAG 1736535 2.225029 7.0602193 75-79
TCGGA 2335000 2.221343 5.7748685 85-89
ATCGG 2334570 2.2209342 5.732948 95-97
CGTGA 2329650 2.2162538 7.1816406 9
CAGCA 1719190 2.2028046 5.462557 2
CATGG 2299590 2.1876569 9.368575 2
GAGCA 1901065 2.1454427 5.9915676 90-94
GCTGG 2083950 2.1401594 7.9293923 4
TCCTT 2948570 2.0963326 5.718943 40-44
ACTGC 1934560 2.089504 6.9207234 4
GATCG 2195075 2.0882292 6.315501 95-97
GCACA 1611070 2.06427 6.531101 95-97
CAAGG 1814555 2.0478117 6.4879317 10-14
CTCTG 2229145 2.0295894 6.6691666 20-24
CTCTC 1914255 1.9787996 5.0004735 15-19
GGCTC 1678395 1.9569744 5.1177506 10-14
CTTGG 2437050 1.9543489 6.0222826 2
CACAC 1338930 1.9477904 7.435823 95-97
CCTTA 2300805 1.940527 6.917419 95-97
AAGGA 2100620 1.9342173 5.8336 10-14
AGATC 2166885 1.909566 5.919674 95-97
GCTAC 1760595 1.9016057 11.512498 1
TGGCT 2368530 1.8994007 7.2262936 9
TTCCA 2231670 1.8822176 5.2870584 75-79
TAGCT 2530805 1.880037 5.439993 6
CATCC 1532495 1.8792832 6.8338313 90-94
TTTCA 3213095 1.8638464 7.0335646 8
TATTC 3208340 1.8610879 5.8509574 2
CCAGC 1171840 1.8402746 6.3427873 1
GTGGC 1777625 1.8255721 8.593819 8
CACAA 1537440 1.8248212 6.3514013 85-89
TGCTG 2258390 1.8110759 5.3219457 6
GCTTC 1989000 1.8109423 7.0891733 15-19
GCATG 1901865 1.8092912 5.1167574 3
TTCGG 2251540 1.8055826 8.496718 7
CTCTA 2109760 1.7793972 5.3386908 95-97
CACCC 996665 1.777034 6.96975 50-54
CCTGT 1939480 1.7658554 7.7774277 1
TGAAC 1980385 1.745213 5.951833 80-84
TCGGT 2168260 1.7387975 9.353777 8
TTGGT 3148885 1.7367655 15.308171 7
GCACC 1097940 1.7242209 6.3421535 50-54
GCAGG 1411865 1.7200582 5.1493964 90-94
TGTAG 2617030 1.7123172 6.358765 3
GTGTA 2603080 1.7031896 8.869972 2
CATGC 1549110 1.6731822 6.1068287 2
CTTTC 2339840 1.6635463 8.233443 7
CCACA 1132230 1.6470964 7.011929 1
CCCAA 1111265 1.6165977 32.187775 2
TGGTA 2465940 1.6134592 9.446455 2
GTAGC 1683895 1.601931 6.8401804 5
GTCTG 1996070 1.600713 6.063332 90-94
GCTTG 1994935 1.5998029 5.6303515 1
GAACA 1523690 1.5928929 5.8974915 80-84
CGCAG 1119535 1.5485308 5.9577727 90-94
ACACA 1298305 1.5409865 6.0974092 85-89
GCTGA 1617990 1.5392339 5.217301 80-84
TCCAA 1538045 1.5388633 5.4234357 75-79
TACTT 2651205 1.5379062 7.360319 5
CCAAT 1524165 1.524976 22.490192 3
CCGCC 773230 1.4882817 5.200771 45-49
ATGCC 1376420 1.4866612 7.115652 9
ACACG 1156560 1.4819047 6.329976 95-97
AACAC 1246545 1.4795516 6.1451917 85-89
GCAAC 1152950 1.4772793 5.708476 1
ATCTC 1736265 1.464387 5.1944013 45-49
GAGCC 1037160 1.4345902 5.1415405 95-97
ACGGT 1496215 1.4233865 6.8798532 4
GTATT 2757390 1.4088104 5.7569256 4
ACATG 1585115 1.3968816 8.992122 1
GCTGT 1704765 1.367106 5.4258437 7
ACTTT 2343865 1.3596249 7.3051877 6
GGTAC 1429030 1.3594718 11.084618 3
GGTCA 1404880 1.3364972 21.283825 9
GACGG 1089700 1.3275684 8.532249 3
TGGTC 1596875 1.2805856 18.197754 8
TGTAT 2463485 1.2586482 5.534696 3
AACGC 972095 1.2455491 5.1319203 95-97
GCGGC 827485 1.2355839 5.589014 7
ATGGG 1468240 1.2302507 7.523967 3
ATTCG 1655190 1.2295765 6.990006 6
CAATT 1717420 1.1818278 15.586326 4
GGTCG 1133290 1.1638577 7.0848126 6
CTGAC 1076225 1.1624225 7.8768873 1
TGGGT 1629310 1.1508249 5.7399726 4
GTACT 1546440 1.1487904 8.623589 4
TGACG 1203670 1.1450812 6.63767 2
ATTGG 1669840 1.0925727 14.860702 6
ACTTG 1451540 1.0782928 6.4076214 8
GGGTC 1015435 1.0428239 7.022565 5
TGGCC 878105 1.0238527 8.327368 1
TCGTG 1224520 0.9819822 5.5868354 8
CGGTA 987070 0.9390241 6.3982534 5
AATTG 1534425 0.9300176 12.773544 5
CTTGT 1350490 0.8456835 5.26991 9
GTCGT 829825 0.6654634 5.3523483 7
CCCCG 326070 0.6276064 5.1788697 1
TGACC 564840 0.6100795 5.9033027 1
Produced by FastQC (version 0.10.1)