FastQCFastQC Report
Mon 21 Jul 2014
20S15B_CTTGTA_L003_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 20S15B_CTTGTA_L003_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 10385242
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[OK] Per base sequence content

Per base sequence content

[WARN] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 198359 1.910008452378866 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 88033 0.847674035905952 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 73746 0.7101038184762569 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 71070 0.6843364844073927 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 68829 0.6627577864820098 No Hit
GACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCT 59878 0.576568172412352 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 54644 0.5261697320100966 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 51081 0.4918614318279728 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 48794 0.46983979766672745 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 48202 0.4641394008921506 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 44190 0.42550765788606565 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 40431 0.3893120641772238 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 37846 0.3644209735314786 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 36805 0.3543971339329406 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 36237 0.34892783432490065 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 34730 0.33441685807610455 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 33658 0.3240945179707897 No Hit
TGACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTC 32496 0.3129055634909615 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 31247 0.30087888178243705 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 29933 0.28822631191454184 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 29814 0.28708045513046304 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 29103 0.2802342015718074 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 27874 0.268400100835397 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 26572 0.25586307955077026 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 25952 0.24989306941523368 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 25947 0.24984492417220514 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 24210 0.23311926674409708 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 23097 0.22240213564594835 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 22540 0.21703875557257118 No Hit
ACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGACTG 22408 0.2157677211566182 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 22351 0.21521886538609308 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 22158 0.21336045900519218 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 20794 0.20022643670701173 No Hit
TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC 20518 0.19756881929183742 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 19712 0.18980780611563985 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 19306 0.18589841238172397 No Hit
CCACGTAGTTGGTTAGCTACTTCTCACTTTGTATTAGGATTTTTCTTCTT 19157 0.1844636841394741 No Hit
GTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATATTGATGGTAT 18782 0.18085279091233503 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 18526 0.17838775446927477 No Hit
GCTAAAAACTATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGA 18180 0.17505610365170113 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 17483 0.16834465677352534 No Hit
TTCATGCGTTGGAGAGACCGTTTCTTATTCTGTGCAGAAGCTATTTACAA 17295 0.16653439563565298 No Hit
GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA 16857 0.16231687234635458 No Hit
TACAACATCGTAGCTGCTCACGGTTACTTTGGTAGATTAATTTTCCAATA 16826 0.16201837183957774 No Hit
ATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTG 16517 0.15904299582041517 No Hit
TACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAAC 16271 0.15667424986341194 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 15927 0.15336185714304973 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 15905 0.15315001807372422 No Hit
CATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGC 14754 0.14206698312855878 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 14511 0.13972712431737266 No Hit
ACCCAATTTGGGAAGCTGCTTCCGTTGATGAATGGCTTTACAACGGTGGT 13891 0.1337571141818361 No Hit
ATACACTTGGGTAACTTATTATTATTTTACAAACCAAGATTTACCATGAC 13424 0.12926034848297227 No Hit
GGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCAT 13383 0.12886555749013842 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 13085 0.12599610100563857 No Hit
GCGACTGGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGT 13034 0.12550501952674767 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 12411 0.11950612224539399 No Hit
TACCATGACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTC 12233 0.11779215159357867 No Hit
ATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGATTTCACTAAA 12116 0.11666555290671128 No Hit
GTATGGGCTTGGATGTTCTTATTTGGACATCTTGTTTGGGCTACTGGGTT 11601 0.11170659287477365 No Hit
ACTCTATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCC 11425 0.11001188032016972 No Hit
GGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTATTT 11162 0.10747944053686952 No Hit
ATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAA 10982 0.10574621178784278 No Hit
CAAGGTGAAACAGGTGAAATTAAAGGGCATTATCTAAATGCTACTGCAGG 10945 0.10538993698943173 No Hit
ACACTTGGGTAACTTATTATTATTTTACAAACCAAGATTTACCATGACTG 10787 0.10386854730973047 No Hit
CCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGATT 10435 0.10047912220052262 No Hit
GCTCATGGTAAGGCTTTATATGGTTTTGATGTTCTTTTATCATCTGCAGA 10432 0.10045023505470552 No Hit
TACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGA 10414 0.10027691217980284 No Hit
GCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATG 10410 0.10023839598538004 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 3892080 3.770436 5.3369546 70-74
CTGCT 3298815 3.5038733 10.72112 4
ACTTC 3386785 3.388515 7.010738 60-64
AACTT 3769890 3.0958335 5.9965 60-64
AGCTG 2732155 3.0315058 7.7338305 3
GGAAG 2535705 2.9391003 6.5327854 15-19
CTACT 2912065 2.9135525 12.874153 2
AGCAC 1900555 2.9013796 5.1027794 35-39
CTTCT 3344260 2.7909687 5.9267774 40-44
CAACT 2321440 2.784493 8.17037 9
TCAAG 2586835 2.7036664 5.63903 10-14
CAAGC 1749750 2.6711617 8.516472 75-79
TGCTA 3054490 2.6629086 7.275153 4
TTCAA 3238645 2.6595752 7.5476003 55-59
CTGGT 2859410 2.6464436 10.312131 1
CTTCA 2640550 2.641899 6.9018555 60-64
GCTGC 1945855 2.6304884 8.702056 3
TCTCT 3116950 2.6012661 8.832828 20-24
ACAAC 1761410 2.532888 8.209475 85-89
TCAAC 2093350 2.5109062 7.4288397 55-59
ACTGC 1965570 2.5029135 10.33606 2
AGGAA 2291470 2.5018497 6.6128035 40-44
CTGAA 2364485 2.4712746 6.609086 80-84
CAGCA 1618045 2.470101 6.1912794 2
ACCCA 1396720 2.4470212 40.779434 1
GCAGC 1480125 2.3987842 6.9687037 1
CGGTG 2030920 2.3922937 10.666851 9
ACATC 1994205 2.3919852 6.51043 90-94
TGGTG 2935855 2.367645 7.8492794 8
GGTGT 2901105 2.3396206 9.175092 9
AAGCT 2234000 2.3348963 5.7375336 15-19
CAACA 1623675 2.3348265 7.880571 85-89
GCTAC 1830680 2.3311474 16.17265 1
TATGC 2665745 2.3240001 7.53231 8
GAAGC 1731945 2.303849 7.480489 15-19
GGTGG 2217350 2.2758923 8.428008 7
CCAAG 1473980 2.250172 7.76138 75-79
TGAAA 2572820 2.2070947 5.0452237 70-74
ATGGT 2887490 2.1934807 5.59347 1
CAAGG 1639395 2.1807382 7.03258 10-14
CAGCT 1693975 2.1570702 5.843597 2
CGGAA 1615205 2.1485605 5.8328004 95-97
CTGCA 1682240 2.1421273 5.1687746 95-97
CTTTA 3118680 2.136254 6.1407185 5
ATGCA 2018375 2.1095328 5.818027 1
CCGTG 1554580 2.1015465 5.595194 60-64
CATGG 1893575 2.101046 13.655406 2
CCAGC 1127685 2.0974174 6.6289315 1
CGTGA 1884785 2.091293 11.3305235 9
TCGGA 1872465 2.0776231 6.0716825 85-89
ATCGG 1867935 2.0725968 5.8022914 85-89
CTCCT 1688480 2.0582144 7.2878294 70-74
TCCTT 2452725 2.046934 5.8608074 40-44
CTCTG 1917130 2.0363011 7.6991925 20-24
CTTGG 2188365 2.0253775 7.1996503 6
GCTGG 1707200 2.0109723 8.643949 4
GCTTC 1876705 1.9933633 8.021218 15-19
GCTCC 1282205 1.9892421 5.6857085 70-74
GTTCC 1827370 1.9409616 5.0451555 70-74
TTTCA 2809210 1.9242713 5.738529 8
TAGCT 2205675 1.9229106 5.666908 2
AAGGA 1749130 1.9097176 6.3455186 10-14
GGTAT 2461490 1.8698698 6.4379716 6
CACAA 1290045 1.8550702 7.10225 85-89
TTCCA 1849330 1.8502748 5.658367 75-79
CCTTA 1840415 1.8413552 7.752425 95-97
TGGCT 1987425 1.8394033 6.254515 9
CCTGT 1721060 1.8280432 7.622312 1
CCGCC 800410 1.813771 6.651402 45-49
TTCGG 1958195 1.8123504 8.302075 7
TGCTG 1957800 1.8119848 5.6233664 6
GCACA 1174135 1.7924298 5.024522 95-97
CATCC 1220945 1.784258 7.303238 90-94
TATTC 2597520 1.7792665 5.444797 5
CTATG 2040555 1.7789587 5.4731746 35-39
CCCAA 1012215 1.7733775 40.81724 2
GCCTT 1657520 1.7605534 5.0903025 35-39
TCGGT 1898225 1.7568469 9.807941 8
CTTTC 2104410 1.7562459 6.8179045 7
TTGGT 2674175 1.6944796 16.789299 7
TACTG 1932350 1.6846254 5.284717 3
CACCC 787815 1.6816111 5.846401 50-54
TGTAG 2208695 1.6778343 5.6547823 3
CCAAT 1394995 1.6732519 28.072372 3
CACAC 946010 1.6573877 5.748196 95-97
TACTT 2404905 1.6473278 7.6179323 3
GCACC 885120 1.6462628 5.3001404 50-54
CCACA 939625 1.6462014 7.7809644 1
TGAAC 1574345 1.6454486 6.2512293 80-84
AGAAA 1597620 1.6430541 7.4392853 9
TGGTA 2150690 1.6337708 8.158359 2
GTGGC 1386385 1.6330729 7.3907638 8
AATGA 1870485 1.6045963 5.209698 75-79
CTGTA 1835230 1.599956 6.862621 8
ACGGT 1433020 1.5900298 9.5105715 4
GCTTG 1714460 1.5867685 6.1809855 1
GAACA 1249705 1.5658811 6.275327 80-84
GTGTA 2060785 1.5654745 8.456386 2
CATGC 1224650 1.5594423 6.7958145 2
AGCCT 1221355 1.5552464 6.052319 2
ACACA 1079860 1.5528266 6.806125 85-89
GCAAC 1017045 1.5526168 7.5592756 1
ATGCC 1214885 1.5470078 10.199736 9
TCCAA 1281040 1.5365665 5.979754 75-79
GCTGT 1658540 1.5350134 5.824514 7
TTTAG 2568470 1.533036 5.151608 6
GCTGA 1369990 1.520094 5.651787 80-84
TTAGG 1988765 1.5107645 5.398524 35-39
GTAGC 1355265 1.5037557 6.3231125 1
ACTTT 2156890 1.4774408 7.817906 4
AACAC 1026185 1.4756427 6.9185505 85-89
CATGA 1397890 1.4610243 5.06776 65-69
AAGCC 950745 1.4514037 6.97055 1
ATGGG 1494860 1.4452711 11.647882 3
CGCAG 887925 1.4390273 5.2689214 90-94
ACATG 1375015 1.4371161 13.908237 1
GGTCG 1215250 1.4314867 11.559528 6
ATCTC 1417910 1.4186343 5.537713 45-49
ATTCC 1407710 1.408429 5.0783405 80-84
GGTAC 1268630 1.4076284 9.299107 3
GACGG 991640 1.4003693 12.019449 3
GTCAA 1312115 1.3713753 5.2321296 10-14
GTATT 2282755 1.362502 5.395039 4
GTCTG 1465830 1.3566563 5.1916804 90-94
GGCTG 1145970 1.3498793 5.6918817 9
GGTCA 1192710 1.3233902 23.584408 9
GCGGC 768800 1.3227409 7.118042 7
GACTG 1189005 1.3192793 8.27507 1
TTCTC 1574205 1.3137606 5.09518 20-24
GCCTG 957640 1.2945778 6.262668 6
TGGGT 1603750 1.2933577 8.5871525 4
GGGTC 1090760 1.2848456 11.502987 5
TGGTC 1383080 1.2800695 19.927822 8
GAATC 1213600 1.2684109 5.062623 40-44
GTATG 1651740 1.2547436 6.597936 7
CAATT 1525365 1.2526298 19.377483 4
CTGAC 966640 1.230898 10.974939 1
TGCCT 1153230 1.2249162 5.0029287 30-34
TGTAT 2044635 1.2203759 5.442298 9
GTACT 1366265 1.1911117 7.2726164 4
TGACG 1072035 1.1894934 9.343229 2
CACAT 982835 1.1788791 5.263606 2
TGGGG 1113585 1.1429858 5.3683157 8
ATTCG 1294465 1.1285164 6.638021 6
ATTGG 1443335 1.0964289 16.987907 6
TGGCC 801805 1.0839136 8.731343 1
CTTAT 1560065 1.0686237 5.3216596 95-97
TCGTG 1137010 1.0523264 9.177328 8
TGCGG 882130 1.0390927 5.213098 6
CGGTA 922970 1.024096 8.994553 5
TAGAA 1135120 0.9737631 5.911614 8
AATTG 1356475 0.9706362 15.431076 5
GCCTA 759460 0.9670795 5.8429794 3
CGGCT 712130 0.96268713 5.4531226 8
TTAGA 1120205 0.80157137 5.392212 7
GGCCT 588230 0.795194 6.0365024 5
CTACA 654635 0.7852137 5.970927 3
CACGG 465200 0.75393254 5.0071955 4
GTCGT 803175 0.7433552 8.938521 7
GGCCC 350030 0.69114774 5.1979694 1
Produced by FastQC (version 0.10.1)