FastQCFastQC Report
Mon 21 Jul 2014
20S15A_CTTGTA_L002_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 20S15A_CTTGTA_L002_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 12208161
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 442346 3.6233630929343086 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 219779 1.8002629552477234 No Hit
GACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCT 166259 1.3618676883438874 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 142117 1.1641147262065106 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 116167 0.9515519986998862 No Hit
TGACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTC 83631 0.6850417519886902 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 80472 0.6591656188020456 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 77480 0.6346574230139986 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 55016 0.4506493647978594 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 48669 0.3986595524092449 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 47604 0.3899358797774702 No Hit
ACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGACGG 43066 0.3527640240000111 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 40935 0.33530848749455383 No Hit
GCTAAAAACTATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGA 40654 0.33300674851847056 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 36182 0.2963755147069243 No Hit
GTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATATTGATGGTAT 36034 0.2951632109045744 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 33843 0.27721619988465096 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 32876 0.2692952689598376 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 32202 0.2637743719140008 No Hit
CACAGTCAATTTGTCTTCCTGCCCTCCATCCAGCGTTTCAGTAGTACCGC 30080 0.2463925565857134 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 27755 0.22734791915014885 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 27487 0.22515266631886652 No Hit
ACCCAATTTGGGAAGCTGCTTCCGTTGATGAATGGCTTTACAACGGTGGT 26831 0.2197792116273696 No Hit
CTGCTGCTATCATCCCTACTTCTGCAGCTATCGGTTTGCACTTCTACCCA 26689 0.2186160552764663 No Hit
CATCTTTTCAAGAGTTATTAATTGAGTTTTAGGGAGGGATTTATGTCACC 26333 0.21569997315730027 No Hit
AACAAGTTTATACACTTGGGTAACTTATTATTATTTTACAAACCAAGATT 26274 0.21521668988474185 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 26214 0.21472521537027567 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 25489 0.20878656498714263 No Hit
GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA 24750 0.2027332372173008 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 24179 0.19805603808796426 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 23973 0.19636864225496373 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 22356 0.18312340409010006 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 22090 0.1809445337426333 No Hit
TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC 21233 0.17392463942767467 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 20824 0.17057442148739685 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 20710 0.16964061990991108 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 20320 0.1664460355658809 No Hit
ACTCTATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCC 20057 0.16429173894413746 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 19791 0.1621128685966707 No Hit
GCTGCTATCATCCCTACTTCTGCAGCTATCGGTTTGCACTTCTACCCAAT 19569 0.16029441289314583 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 18954 0.15525679911986745 No Hit
ATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTG 18349 0.15030109776566675 No Hit
AGTATGCTTCAAAAATACCTTTGGTATTACATTGACAATCTAACGAGGAT 18330 0.15014546416941912 No Hit
GGGTAACTTATTATTATTTTACAAACCAAGATTTACCATGACTGCTACTT 18094 0.1482123310791855 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 17512 0.1434450282888635 No Hit
TACAACATCGTAGCTGCTCACGGTTACTTTGGTAGATTAATTTTCCAATA 17115 0.1401931052514789 No Hit
ACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGAT 15605 0.1278243299707466 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 15559 0.1274475328429892 No Hit
TACCCATTAGATTTATTTGAAGAAGGTTCTGTTACTAACTTATTTACTTC 15407 0.1262024640730082 No Hit
GGTTCACTGCATTAGGTATCAGCACTATGGCTTTCAACTTAAACGGTTTC 15304 0.12535876615650793 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 15213 0.12461336314290089 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 14978 0.122688421294575 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 14824 0.12142697004077846 No Hit
ATACACTTGGGTAACTTATTATTATTTTACAAACCAAGATTTACCATGAC 14524 0.11896959746844754 No Hit
GCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATG 14490 0.11869109524358337 No Hit
CTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGC 14169 0.11606170659118928 No Hit
CCTACTCTATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGC 14062 0.11518524370705792 No Hit
ACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGACTG 14040 0.115005036385087 No Hit
GTATGCTTCAAAAATACCTTTGGTATTACATTGACAATCTAACGAGGATT 13985 0.11455451808015965 No Hit
GCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGT 13610 0.111482802364746 No Hit
CCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCCAGAA 13588 0.11130259504277508 No Hit
CATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGC 12454 0.10201372671936419 No Hit
CCACGTAGTTGGTTAGCTACTTCTCACTTTGTATTAGGATTTTTCTTCTT 12401 0.10157959089825241 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 12396 0.10153863468871356 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 6948880 5.334276 15.487112 70-74
AGCTG 3811310 3.6961322 11.107474 80-84
CAAGC 2749010 3.4784093 14.0282755 30-34
CTGCT 3780765 3.4204311 19.712921 3
ACTTC 4041860 3.3051221 9.984078 60-64
GGAAG 2946670 3.0632098 11.34766 15-19
ACCCA 2159015 3.013975 70.85034 1
CTTCT 4331005 2.9946315 8.442492 20-24
CTGGT 3628365 2.9753122 20.99535 1
AACTT 4441150 2.9752765 8.200682 60-64
ACAAC 2549095 2.9153826 13.684642 85-89
AAGCT 3303430 2.8956327 9.725942 75-79
CTGAA 3299225 2.8919466 9.984681 80-84
AGGAA 3031200 2.8481638 10.604192 40-44
CCAAG 2241765 2.8365765 13.013668 75-79
TCTCT 4026730 2.7842436 10.274585 20-24
CAACT 2857285 2.7631946 17.379438 9
AGCAC 2148140 2.7181098 8.308613 65-69
TGGTG 3636565 2.7029212 6.338409 8
CTTCA 3293795 2.6934125 9.796029 60-64
CAACA 2341695 2.6781805 12.988688 85-89
ACATC 2763245 2.6722512 10.347496 90-94
GGTGT 3489255 2.5934312 7.656388 70-74
TCAAG 2940475 2.5774832 9.4027405 10-14
TTCAA 3807075 2.550488 16.543718 9
TCAAC 2626350 2.5398643 15.513839 8
GCTGC 2142685 2.5363314 11.161623 3
CGGTG 2363245 2.5355773 9.854416 85-89
CTACT 3079180 2.517917 16.390694 6
GAAGC 2192045 2.5140524 12.515354 15-19
TATGC 3370995 2.4985316 9.543662 30-34
GGTGG 2522225 2.4528575 6.0293674 90-94
GCTTC 2632750 2.38183 11.400249 15-19
GCTAC 2225860 2.3815002 20.750608 5
CTTTA 4187560 2.3721454 10.338201 9
CAGCA 1866370 2.3615773 9.456215 60-64
TTCCA 2877795 2.3532398 9.206621 75-79
TGCTA 3156405 2.3394804 15.046324 4
GCAGC 1644130 2.3016295 7.7634478 1
CTCTG 2533310 2.2918673 11.421334 20-24
CACAA 1994825 2.281468 11.789049 85-89
CAAGG 1988310 2.2803888 12.008937 10-14
ACTGC 2101165 2.248086 21.673286 2
TCTGC 2466145 2.231104 6.3304 45-49
GGTTC 2705685 2.2187011 5.8001842 90-94
CCAGC 1433175 2.2134926 9.73479 60-64
GCTGG 2055740 2.2056482 9.4393425 75-79
CATCC 1851760 2.1858335 11.892644 90-94
TCCTT 3135840 2.168246 7.592311 90-94
GTTCC 2384530 2.1572673 9.012514 70-74
GAAAG 2270625 2.1335154 7.3626285 10-14
CTTGG 2598315 2.1306565 7.4970045 65-69
TGAAA 2953725 2.1211627 6.751122 65-69
ATGCA 2394385 2.0988061 6.161952 1
CCCAA 1483320 2.0707078 70.02089 2
AAGGA 2198955 2.066173 10.403825 10-14
ACACA 1791440 2.0488577 11.599667 85-89
CAGCT 1902335 2.0353532 6.8224854 2
TTTCA 3574445 2.0248313 14.174266 8
CCTTA 2456055 2.008373 13.950354 95-97
TTGGT 3524275 2.002006 29.953587 7
ATCGG 2049245 1.9873168 7.664096 85-89
GCCTT 2188300 1.9797395 9.070201 35-39
GAACA 1897750 1.9672925 10.750952 80-84
AACAC 1717460 1.9642475 11.689089 85-89
TGAAC 2234135 1.9583385 9.436194 80-84
CACCC 1145595 1.9520437 13.600791 50-54
TACTT 3438385 1.9477568 15.841421 3
GATGG 2211410 1.9438508 6.100464 90-94
GAAAA 2286555 1.9419461 7.1126585 70-74
GCACC 1249350 1.9295808 12.496595 50-54
GCTGA 1989400 1.9292802 10.014795 80-84
TCGGA 1987790 1.9277188 8.028161 85-89
AGAGC 1679665 1.9264046 5.2130594 95-97
GCTTG 2343535 1.9217329 9.382153 65-69
TCCAA 1983125 1.9178207 9.877711 75-79
TGCTT 3059655 1.9175537 6.8813934 65-69
CTCTC 1912965 1.9093596 8.248134 95-97
TGGCT 2321665 1.9037992 7.1774335 90-94
CTTTC 2746850 1.8992828 17.017477 7
CCGCC 1002880 1.8906114 10.932101 75-79
CCACA 1347775 1.8814876 11.400543 60-64
TCGGT 2280815 1.8703016 7.5104637 95-97
TTCGG 2277215 1.8673499 7.664922 95-97
CATGG 1918525 1.8605472 12.026382 2
ACCAG 1468490 1.858127 8.151507 60-64
CATGA 2105165 1.8452895 8.783635 65-69
TCTGG 2245070 1.8409904 8.55035 45-49
GGTAT 2726615 1.8317703 7.7345033 30-34
TGGTA 2725485 1.8310109 17.04689 2
ATGCC 1701200 1.8201541 10.554112 30-34
GGCTC 1536995 1.8193662 9.557498 90-94
GCAAG 1579475 1.8114967 8.4901905 25-29
GTGTA 2695215 1.8106753 6.1396046 2
CGTGA 1859860 1.8036549 8.533007 9
GGTAC 1858470 1.802307 23.353746 3
ACTGA 2052990 1.7995553 5.5718293 65-69
AGAAA 2112880 1.7944459 12.236823 9
AAAGA 2111110 1.7929426 5.826295 15-19
CATGC 1658285 1.7742382 6.887343 2
ACTTT 3131980 1.7741861 16.136734 4
CCAAT 1826645 1.7664937 48.769913 3
GCACT 1650505 1.7659142 6.5744133 65-69
ACGGT 1803975 1.7494589 9.529558 25-29
TGTAG 2576205 1.730723 5.952544 70-74
AGCTT 2328260 1.7256719 8.020066 15-19
CCTTT 2492890 1.7236847 7.1292195 35-39
TTTAG 3356615 1.723464 8.519445 6
GTGGC 1599385 1.716015 5.7144914 90-94
AACAT 2162830 1.7135859 8.303872 90-94
TGCTG 2084135 1.7090211 5.548996 3
GTAGC 1738800 1.6862534 5.2494435 75-79
AAAAC 1796295 1.6831111 6.775577 70-74
TGCAC 1569450 1.6791916 8.873848 50-54
CACTG 1544025 1.6519887 7.0486403 65-69
AAACC 1438440 1.6451341 7.3225303 70-74
CTATG 2218025 1.6439672 6.264043 35-39
TCATG 2210100 1.6380932 7.6271873 60-64
AGCCT 1522970 1.6294618 6.7206407 30-34
ATGAT 2675030 1.6243551 6.4943466 65-69
AAAGC 1565580 1.6229504 7.495388 20-24
GTGTT 2851165 1.6196376 7.0549235 70-74
GACGG 1275310 1.6182122 12.507413 25-29
GGTCG 1493015 1.6018884 9.345288 6
GACTG 1649110 1.5992739 18.103968 1
TTCAT 2820515 1.5977492 6.428367 60-64
AAGCC 1256960 1.5904714 7.6729293 30-34
ACCGC 1026695 1.5856974 8.813813 75-79
ACATG 1808840 1.5855447 11.639195 1
TTACA 2363230 1.5832076 5.456679 80-84
GATCG 1627725 1.5785351 5.747458 8
TTAGG 2344735 1.575219 8.464509 35-39
GCTCT 1737875 1.5722431 7.3531604 90-94
ATGGG 1782965 1.5672435 9.17723 3
CTGCG 1311275 1.5521777 8.284071 45-49
GGATG 1764085 1.5506477 6.668344 85-89
ATCTC 1893980 1.5487511 10.37404 45-49
TGGTC 1855670 1.5216765 38.774334 8
CTCTA 1818745 1.4872297 10.702198 95-97
GTCAA 1691655 1.4828258 8.864925 10-14
GCTGT 1802860 1.4783715 5.155309 4
GTACT 1992525 1.47683 17.998367 4
TCCAC 1247925 1.4730612 6.245077 60-64
AATCT 2185300 1.4640063 7.489039 40-44
GGTCA 1509365 1.463752 45.562984 9
GGGTC 1354175 1.4529239 9.33124 5
GCGAC 1036880 1.4515358 8.15504 50-54
GTATG 2159505 1.4507793 6.9379926 30-34
GATTG 2157605 1.449503 6.896999 65-69
ATCCT 1766295 1.4443403 8.187249 90-94
TTCTC 2083160 1.4403808 7.452642 20-24
AAACT 1810300 1.4342803 5.764037 6
GGAAT 1801415 1.4312402 8.1716385 40-44
GACGC 1014440 1.420122 8.634064 15-19
TGGGT 1901480 1.4132981 7.0603495 4
ACTGG 1456850 1.412824 6.791152 50-54
ATGCT 1905910 1.4126322 5.325754 3
GCGCA 1003565 1.404898 8.62141 25-29
TACCA 1451295 1.4035039 5.755807 60-64
CTGGG 1305965 1.4011983 6.410635 55-59
TCTGA 1876705 1.390986 8.500079 20-24
TTGTG 2448540 1.390922 5.9830337 70-74
CATCG 1298615 1.3894188 6.343641 85-89
CAATT 2073625 1.3891914 34.202625 4
AGCGC 991005 1.3873153 8.155968 25-29
GAATC 1576330 1.3817374 8.758964 40-44
GCGAA 1197995 1.3739781 6.847919 20-24
ATTCC 1679715 1.3735418 6.87344 55-59
CTGAC 1283280 1.3730116 10.511027 25-29
TGCCT 1511980 1.3678775 8.974407 30-34
CTGTA 1839290 1.3632543 6.153918 8
CCCAT 1149340 1.3566908 6.155627 55-59
TGACG 1381310 1.3395668 9.670929 25-29
TGATT 2590750 1.3302282 5.3450685 65-69
CGCAA 1048890 1.3271939 7.7643924 25-29
CACAT 1371975 1.3267959 7.8957095 60-64
CGCCT 1015460 1.3261421 8.163848 75-79
TGGGG 1362345 1.324877 6.118775 35-39
GCAAC 1045920 1.3234357 8.847023 1
CTTAT 2331005 1.3204545 9.332042 95-97
AAGAC 1272805 1.3194467 6.436741 15-19
AGATC 1502915 1.3173852 5.233203 7
AAGCG 1131315 1.297503 6.75589 25-29
AACCG 1010700 1.2788708 8.447898 70-74
TCGCT 1412740 1.2780958 5.7091613 40-44
TGTTC 2035580 1.2757431 6.4298162 70-74
TGTGT 2243505 1.2744492 5.891927 70-74
AGACG 1103695 1.2658257 7.247025 15-19
CGCGA 889460 1.2451617 7.9564185 20-24
ATTGG 1827595 1.2277987 32.28768 6
GTCGC 1035790 1.2260816 7.144923 40-44
CCATT 1497490 1.2245322 7.2408056 55-59
TAGGA 1539080 1.2228129 7.874786 40-44
GGGGT 1255790 1.2212526 6.634057 35-39
CATTC 1484250 1.2137054 6.784453 55-59
GTTAC 1626150 1.2052783 5.3816104 55-59
CGGTA 1240010 1.202537 9.315857 25-29
TTATG 2334165 1.198484 8.576788 95-97
TGCGA 1213115 1.1764545 5.802686 50-54
CGAAA 1126710 1.167998 6.4869266 20-24
CGGAT 1198990 1.1627566 5.8082085 85-89
CGACT 1080680 1.156245 6.5236216 50-54
ACGCG 822440 1.1513399 9.272895 15-19
ATTGT 2214155 1.1368645 5.276431 65-69
TAGAA 1575135 1.131154 10.150992 8
CGCTT 1224335 1.1076472 6.28973 40-44
GCCTA 1028595 1.1005181 6.407973 30-34
AATTG 1803905 1.0953828 28.796572 5
TCGTG 1270775 1.042054 7.3063006 8
GTTCG 1263305 1.0359287 5.5594387 90-94
TGGCC 834370 0.9876574 8.235553 1
TTAGA 1559145 0.9467576 9.313355 7
CCTAT 1082675 0.8853285 5.555228 30-34
CACAG 632440 0.8002464 6.0760665 1
CTACA 768200 0.74290323 5.863626 3
GTCGT 894860 0.73379827 7.107468 7
TGACT 926485 0.68669695 7.3688745 1
GGCCC 390325 0.6669604 5.0152884 1
Produced by FastQC (version 0.10.1)