FastQCFastQC Report
Mon 21 Jul 2014
20L22C_TGACCA_L004_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 20L22C_TGACCA_L004_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 12478381
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 206219 1.6526102224319006 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 141175 1.1313567040467831 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 129657 1.0390530630536126 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 104300 0.8358456117023514 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 103056 0.8258763696989216 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 92833 0.7439506775758811 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 88663 0.7105328808280498 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 81982 0.6569922812903373 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 80729 0.6469509145457252 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 66479 0.5327534076736397 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 54503 0.4367794187402997 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 51268 0.4108545812153035 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 50225 0.40249612509828003 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 50047 0.40106965799489536 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 47242 0.3785907803263901 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 43621 0.3495725927906833 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 41591 0.3333044567239933 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 36959 0.296184256595467 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 35721 0.2862630977528255 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 34177 0.27388969770998334 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 33771 0.27063607049664534 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 31635 0.2535184652560296 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 31050 0.24883035707917558 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 29669 0.2377632162377475 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 29203 0.23402875741652704 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 28549 0.22878769289060819 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 28273 0.22657586749434883 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 26385 0.21144569956631393 No Hit
GCTCATGGTAAGGCTTTATATGGTTTTGATGTTCTTTTATCATCTGCAGA 24148 0.19351869445242936 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 22136 0.17739480786810405 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 21780 0.17454187366133475 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 21334 0.17096769204274176 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 20851 0.16709699759928792 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 20328 0.16290574875057912 No Hit
GGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTATTT 20198 0.16186394693350042 No Hit
GGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCAT 18860 0.15114140207772145 No Hit
GCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATG 18835 0.1509410555744371 No Hit
GGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTA 18178 0.1456759494681241 No Hit
TACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGA 17982 0.14410523288237473 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 17875 0.14324774984831765 No Hit
GTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAG 17748 0.1422299896116331 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 17517 0.14037878792128564 No Hit
ATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAA 17504 0.14027460773957776 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 16933 0.13569869360456296 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 16775 0.1344325037038058 No Hit
CGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTC 16393 0.13137120913362077 No Hit
TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC 16249 0.13021721327470287 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 16012 0.12831792842356712 No Hit
GGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCTGGTACTTT 15658 0.12548102193706057 No Hit
TGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCT 15472 0.12399044395262494 No Hit
GCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGA 15466 0.12394236079183671 No Hit
CTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCC 15452 0.12383016674999746 No Hit
ACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGACGG 14981 0.1200556386281201 No Hit
GTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGG 14980 0.12004762476798872 No Hit
GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA 14756 0.11825252009856085 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 14743 0.11814833991685299 No Hit
CTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGT 14692 0.11773963305015289 No Hit
GTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCC 14106 0.1130435110131675 No Hit
ATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCA 13590 0.1089083591853783 No Hit
GTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCTTTCAA 13394 0.1073376425996289 No Hit
CATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGC 13359 0.10705715749503081 No Hit
ATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTG 13263 0.10628782692241887 No Hit
GTATGGGCTTGGATGTTCTTATTTGGACATCTTGTTTGGGCTACTGGGTT 13029 0.10441258365167724 No Hit
ATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGGAT 12836 0.102865908646322 No Hit
ATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCATCTC 12800 0.1025774096815925 No Hit
GGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGGATCG 12613 0.10107881783702549 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 5890080 4.742856 7.662229 45-49
ACTTC 4674615 3.8341556 7.444262 60-64
CTGCT 4251910 3.7530615 12.003591 5
AACTT 5283500 3.5269918 7.1911 55-59
AGCTG 3540720 3.332486 10.174634 3
TCAAG 3564215 3.1171877 5.5429306 60-64
CTTCA 3789145 3.1078868 7.9144025 60-64
CTACT 3731485 3.0605931 12.3182 2
TGCTA 4253300 3.0555296 5.6405067 95-97
CTGGT 3917815 3.0288801 17.328571 1
CAACT 3022685 3.0182512 12.9620285 9
TTCAA 4512400 3.0122457 10.670823 9
GGAAG 2994805 3.0055258 6.1950808 15-19
TCTCT 4336550 2.9216607 9.503093 20-24
ACCCA 1877895 2.804875 36.247288 1
TCAAC 2782355 2.7782738 11.998955 8
AGCAC 2095495 2.7413557 5.6894255 90-94
CGGTG 2693395 2.7280636 16.063148 9
AGGAA 2925220 2.727927 6.9565306 40-44
TATGC 3793415 2.7251527 8.451208 30-34
ACAAC 2226685 2.7068226 7.3232822 85-89
GGTGT 3988905 2.7010238 13.784977 1
TGGTG 3952290 2.6762307 10.725021 6
GGTGG 3000465 2.6618254 12.023611 7
GAAGA 2820100 2.6298966 5.3567924 90-94
GCTGC 2267785 2.622526 9.045514 3
ATGCA 2975000 2.6018727 6.982947 1
GAAAC 2412035 2.568155 6.119108 75-79
GGTTC 3292760 2.5456476 5.160973 3
CTTCT 3773095 2.5420444 6.2081037 40-44
CAAGC 1939580 2.5373855 7.7585664 75-79
CAACA 2080930 2.529639 7.131071 85-89
CCGTG 2177475 2.5180888 7.259017 60-64
AAGCT 2858850 2.50029 5.4127555 15-19
CATGG 2597765 2.4449873 12.234754 2
CGTGA 2585830 2.4337542 10.382482 9
GTTCC 2741840 2.4201577 5.74916 45-49
ACTGC 2250320 2.4181566 12.550961 4
CCAAG 1835375 2.401063 8.208474 25-29
GAAGC 2094345 2.3997364 7.1321163 15-19
CTGAA 2743290 2.3992238 5.956342 80-84
TCCTT 3494915 2.354626 8.082758 40-44
GAGAA 2483945 2.3164139 5.7172246 80-84
TCTGC 2619925 2.3125465 6.6133146 85-89
ACATC 2310465 2.3070757 7.0124464 40-44
GCTGG 2270720 2.299948 11.503076 4
GCAGC 1628785 2.2930853 6.552233 1
CAAGG 1998875 2.2903452 6.7940006 10-14
GGCTC 1974410 2.2832594 6.844456 10-14
ATGGT 3594940 2.2619798 6.4623184 1
CCTTA 2744120 2.2507486 8.880309 45-49
ACTGA 2568705 2.2465355 5.370202 70-74
CAGCA 1717245 2.2465236 6.0052576 35-39
CTCTG 2506210 2.2121727 7.970925 45-49
CTCTC 2193545 2.2106073 6.5015993 15-19
CAAGT 2487835 2.1758082 5.2840724 50-54
GCTAC 2018500 2.1690469 15.6129875 1
CTGCA 2006600 2.1562593 5.189752 95-97
ATCCG 1998945 2.1480334 6.0499926 65-69
TGGCT 2760665 2.1342824 9.169358 9
CGGAA 1846075 2.1152644 6.0848403 95-97
TATTC 3832810 2.1016552 7.964666 5
CTTGG 2717750 2.1011045 7.5617037 2
CAGCT 1948030 2.093321 6.177465 65-69
GTGGC 2061720 2.0882578 11.285706 8
TAGCT 2901820 2.084639 7.3358808 2
TTCCA 2528495 2.0738916 6.4203534 25-29
CTCTA 2527295 2.0729072 7.4862323 95-97
CACAA 1669760 2.0298088 7.4334607 35-39
AAGGA 2167360 2.0211809 6.036133 10-14
CATCC 1637600 2.0091457 7.1116467 40-44
TTTCA 3626405 1.9884764 8.907136 8
TTCGG 2552080 1.9730244 12.16208 7
GCATG 2080905 1.9585245 6.3035817 3
TACTG 2724005 1.9568986 8.614322 3
AAACT 2405730 1.9550965 5.220824 65-69
TCCGT 2210625 1.951267 5.0932474 70-74
CACCC 1061640 1.9483215 10.240236 50-54
AGAGC 1693825 1.9408137 6.5629215 95-97
GGTAT 3055005 1.9222461 5.3917446 30-34
TCGGT 2482815 1.9194752 13.320855 8
GCTCT 2168455 1.9140445 6.3663745 20-24
GTGTA 3031605 1.9075223 12.573733 2
GCTCC 1442060 1.90399 5.7888036 65-69
GCAGG 1539300 1.8980879 7.0920763 90-94
CCCAA 1260455 1.8826497 36.421734 2
TGCTG 2430160 1.8787675 9.377997 6
GCACC 1165355 1.8731743 9.235163 50-54
CTCCT 1858315 1.8727697 7.087536 70-74
AATGA 2614795 1.8612106 6.3276024 75-79
CCAAT 1857140 1.8544159 24.516035 3
ATCGG 1969730 1.8538878 5.0083594 95-97
GAGCA 1617845 1.8537545 5.492371 90-94
GTGAA 2414870 1.8498193 5.3750424 65-69
GATCG 1962705 1.847276 5.4030395 95-97
TTCCT 2726920 1.8372058 5.1815734 45-49
CAGTG 1925150 1.8119296 6.5800433 30-34
CCAGC 1126060 1.8100121 6.628331 1
CTATG 2515410 1.8070459 5.531611 35-39
CTGTT 3049645 1.79958 7.3621874 8
CCACA 1204695 1.799365 8.681193 60-64
CTTTC 2666130 1.7962495 10.583731 7
GTAGC 1906130 1.7940283 8.910524 1
GCTTC 2028845 1.7908139 7.417371 15-19
TTGGT 3432100 1.7738563 14.49151 7
TGTAG 2811655 1.769127 7.1760983 3
AGCTC 1640760 1.7631336 5.030108 65-69
CGCAG 1251750 1.7622768 8.168804 90-94
GCACA 1339510 1.7523656 6.018196 95-97
CCTGT 1984020 1.7512481 8.379482 1
ACACA 1422430 1.7291472 7.289274 35-39
AAGTT 2955585 1.7280755 5.384864 60-64
ACTAC 1730255 1.727717 6.09674 70-74
CTGAG 1834185 1.7263145 6.4842067 80-84
TGGTA 2726500 1.7155466 12.387313 2
AGTGC 1820870 1.7137825 5.774378 25-29
ACGGT 1812580 1.70598 7.762186 4
CTATT 3109890 1.7052544 5.1135206 25-29
AATCC 1698810 1.696318 6.0258894 60-64
GAACA 1591235 1.6942282 5.962645 80-84
TGAAC 1932550 1.6901677 5.6305237 80-84
AACAC 1386275 1.6851958 7.055014 35-39
GCTTG 2147780 1.6604582 7.19995 1
TCCAA 1660845 1.6584089 6.6266894 25-29
GCTGA 1753255 1.650144 5.9211807 30-34
CATGA 1855500 1.6227814 5.428702 15-19
AGATC 1855495 1.622777 5.090358 95-97
ATGAT 2774810 1.6223799 5.4667754 15-19
ATGCC 1507775 1.6202301 8.048562 9
GGTAC 1721170 1.619946 15.4988165 3
ACCAG 1221890 1.5984935 5.1782455 2
CACAC 1067450 1.5943724 6.889748 95-97
TACTT 2893085 1.5863731 9.208754 5
GTCTG 2036530 1.5744504 6.9318395 90-94
CTACC 1277515 1.5673631 5.8359137 20-24
GCTGT 2018715 1.5606775 9.583608 7
GACGG 1252570 1.5445254 10.033788 3
AACAT 1878165 1.5263531 5.7080574 40-44
CTGTA 2109120 1.5151714 5.903579 8
ATTCC 1842970 1.5116183 5.0332932 55-59
AACGC 1150335 1.504884 7.5038695 95-97
TGCAC 1394060 1.4980339 6.382518 50-54
GGTCA 1581455 1.4884478 22.311678 9
GTATT 3080820 1.4796094 7.390958 4
CTGAC 1372235 1.4745812 8.493546 1
ACGCA 1125545 1.4724536 5.6387877 90-94
ACATG 1678780 1.4682257 10.982625 1
AACTA 1797090 1.460465 5.5867195 75-79
ATCTC 1771445 1.452953 5.299246 45-49
AACCA 1194030 1.4514974 5.072742 75-79
CCGCC 715860 1.4138038 6.6394463 45-49
ACTTT 2540445 1.3930091 9.062781 6
TGGTC 1797030 1.3892919 18.496168 8
GGCTG 1368415 1.3860289 5.5799856 9
CAGGT 1469980 1.3835287 6.390805 95-97
GCTAA 1581745 1.383361 5.084436 85-89
ATTCG 1916465 1.3767698 10.142529 6
GCGGC 884695 1.3403794 7.321788 7
TGTAT 2761205 1.3261094 7.173212 3
GTACT 1843235 1.324162 11.699997 4
AGGTA 1686300 1.2917261 5.562698 6
GTATG 2036985 1.2816955 5.298268 7
TGTTT 3240670 1.2784301 5.447133 9
TGACG 1355175 1.2754755 7.526692 2
GCCTG 1102050 1.2744395 7.2325983 6
AGCCT 1178185 1.2660582 6.046471 2
CACAT 1260010 1.2581618 5.810313 60-64
ATGGG 1520345 1.2533011 10.791126 3
CTAAC 1242360 1.2405376 6.0045514 95-97
CAATT 1856985 1.2396274 16.489378 4
GGTCG 1189480 1.2047907 10.624911 6
ACACG 908235 1.1881657 5.8149605 95-97
CCATT 1389110 1.1393588 5.4030476 55-59
ATTGG 1810430 1.1391443 15.332504 6
TGGGT 1662065 1.1254408 7.961023 4
AAGCC 852460 1.1151999 6.181419 1
GCTCA 1033780 1.110883 5.935851 1
CGGTA 1166320 1.0977273 7.1711683 5
GGGTC 1055510 1.0690963 10.580217 5
TGGCC 921205 1.0653055 10.661665 1
TGCGG 1018555 1.0316654 5.244431 6
TCGTG 1292395 0.9991563 8.108428 8
CGGCT 848740 0.98150516 5.406324 8
TAACG 1120880 0.9802981 5.0199513 90-94
TTGGC 1267275 0.97973603 5.0946193 3
AATTG 1654025 0.9670776 13.494663 5
TGGGG 1046360 0.9282652 5.325663 8
GGGGT 915420 0.8121036 5.0275474 9
GGCCT 679330 0.785595 7.117083 5
GTCGT 939620 0.7264244 7.8981166 7
CCCTA 592030 0.7263523 5.228741 2
GCCTA 648060 0.6963946 5.9782114 3
GGCCC 393595 0.68084335 5.2744713 1
GCCCT 494340 0.65269023 6.0550113 1
GGTAA 744900 0.57060236 5.92942 7
Produced by FastQC (version 0.10.1)