FastQCFastQC Report
Mon 21 Jul 2014
20L22B_TGACCA_L003_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 20L22B_TGACCA_L003_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 17082796
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 222368 1.3017072849198692 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 206200 1.2070623567711047 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 182959 1.0710131994785865 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 141980 0.8311285810589788 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 111730 0.6540498405530335 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 106338 0.6224859209230151 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 91530 0.5358022187936916 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 90897 0.5320967363890549 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 88513 0.518141175484388 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 86851 0.5084120889812184 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 84586 0.49515313535325245 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 70125 0.4105007166274186 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 68929 0.40349952080443974 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 63403 0.37115118625779997 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 58580 0.3429181031020917 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 58464 0.3422390573533747 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 56528 0.33090601796099417 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 54953 0.32168621576936235 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 54886 0.32129400831105165 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 54578 0.31949102477135477 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 51811 0.30329344212738946 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 46587 0.2727129680644784 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 43581 0.25511631702444965 No Hit
GGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTATTT 41373 0.24219103242818096 No Hit
GGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTA 38748 0.22682469544212788 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 38614 0.22604028052550648 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 38347 0.22447730453492507 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 35719 0.2090934060208879 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 35394 0.20719090715594798 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 35215 0.20614306931956572 No Hit
GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA 35110 0.20552841584012357 No Hit
CGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTC 35108 0.2055167081548009 No Hit
TAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTA 34605 0.20257222529614002 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 33507 0.19614470605397386 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 33136 0.19397293042661168 No Hit
GGTCCTTACGAGTTAATCGTTCTTCACTTCTTACTTGGTGTAGCTTGCTA 32423 0.18979914060906658 No Hit
ATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGATTTCACTAAA 31696 0.18554339699426253 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 30473 0.17838414741942712 No Hit
GTATGGGCTTGGATGTTCTTATTTGGACATCTTGTTTGGGCTACTGGGTT 30024 0.17575577206447937 No Hit
GTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAG 28168 0.16489104008500716 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 27354 0.16012601215866537 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 27070 0.15846352084284096 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 26523 0.15526146890708056 No Hit
GGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCTGGTACTTT 25316 0.1481958808148268 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 25193 0.14747585816748032 No Hit
ATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCATCTC 24710 0.14464845216204655 No Hit
TACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAAC 24405 0.1428630301503337 No Hit
ATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTG 24333 0.14244155347871626 No Hit
GTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCTTTCAA 23876 0.13976634738247767 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 23772 0.1391575477456969 No Hit
CGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTG 23725 0.13888241714061328 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 23717 0.13883558639932245 No Hit
GGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCAT 23265 0.13618964951639065 No Hit
GTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGG 23019 0.13474960422169766 No Hit
TACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGA 22440 0.13136022932077393 No Hit
CATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGC 22244 0.13021287615914864 No Hit
TGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTATT 21950 0.1284918464167107 No Hit
GCGACTGGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGT 21888 0.1281289081717068 No Hit
CTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGT 21574 0.12629080157604175 No Hit
GTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCATCTCTGCT 20820 0.12187700420938118 No Hit
GCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTC 20810 0.12181846578276764 No Hit
CGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGGATCGCTGT 19437 0.1137811398087292 No Hit
GGCCCTGGAGATTTCTTAGTTCATCACGCTATTGCTTTAGGTTTACATAC 19307 0.11302014026275324 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 19096 0.11178497946120763 No Hit
CGTGGTGGACTTGATTTCACTAAAGATGATGAAAACGTAAACTCTCAACC 18910 0.11069616472619587 No Hit
GGGTAACTTATTATTATTTTACAAACCAAGATTTACCATGACTGCTACTT 18815 0.11014004967336728 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 18478 0.10816730469649113 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
ACTTC 5921070 3.649082 6.46051 40-44
CTGCT 5363490 3.4610112 16.447695 5
AAAAA 5399435 3.312583 6.8327637 45-49
AACTT 6456745 3.244357 5.2310915 55-59
CAACT 4159020 3.1366093 7.0357056 9
CTACT 5014170 3.090171 15.989372 2
ACTGC 3532100 2.7891667 17.80921 4
CTTCT 5538255 2.7891407 8.973012 40-44
GGAAG 3924350 2.763772 5.0198584 7
CTTCA 4456555 2.7465196 5.8417444 60-64
AGCTG 4063850 2.7395718 8.448548 3
TTCAA 5402880 2.7148151 6.700943 55-59
AGGAA 4013690 2.6996427 6.0112324 40-44
TCAAC 3542605 2.6717274 6.470944 55-59
ACCCA 2329925 2.6373389 29.518307 1
TCTCT 5221595 2.6296663 7.5452495 20-24
GCAGC 2574025 2.6044273 9.298095 65-69
CTGGT 4696720 2.587341 11.017148 1
GCTGC 3086815 2.5522554 8.468405 3
GAAGC 3017650 2.4894297 5.79608 35-39
CTGCA 3139450 2.4791057 7.6278343 35-39
ACAAC 2670430 2.4645443 5.854077 85-89
AGCAC 2541235 2.455683 5.726918 35-39
GGTTC 4386830 2.4166281 11.358632 3
CAGCA 2475045 2.391721 5.8515725 35-39
CGGTG 3378535 2.3847642 15.761868 7
CAAGG 2872570 2.369745 5.9044857 4
TATGC 4375550 2.3020759 6.128082 30-34
CTCCT 3042210 2.299541 10.503247 70-74
CAAGC 2370145 2.2903528 6.3191257 75-79
GGTGT 4860920 2.2860253 12.718186 8
CAACA 2475240 2.284403 5.7888155 85-89
ATGGT 5043300 2.2651937 11.959236 1
CAGCT 2844970 2.2465658 7.6382074 65-69
GGTGG 3715925 2.2391725 9.328741 7
TGGTG 4710930 2.215487 8.498571 6
CCGTG 2673835 2.210793 7.540922 95-97
TGCAG 3267090 2.2024503 7.7370143 65-69
CTCTG 3405850 2.1977637 7.7672615 45-49
GCTCC 2258860 2.1877582 9.794008 70-74
GCTAC 2763530 2.1822557 20.61359 1
CCAAG 2219230 2.1445181 5.9050946 75-79
TCTGC 3287695 2.121519 5.193796 85-89
CATGG 3131915 2.1113245 10.593459 2
ATGCA 3228390 2.0785427 5.5934076 1
CGTGA 3053835 2.058688 8.605929 9
TTTCA 4989770 2.048847 5.3099174 8
TGGTT 5581820 2.0487096 7.867214 2
AGCTC 2575400 2.0336967 7.8562107 70-74
CCCAA 1757130 1.9889681 30.170368 2
TCCTT 3897565 1.9628668 5.62206 40-44
ATCCG 2484830 1.9621769 9.279908 95-97
GCAAC 2002245 1.9348384 8.059187 35-39
CCAGC 1629115 1.9308462 6.7989926 30-34
CCTAC 2077980 1.9221146 7.3307424 20-24
GTTCC 2977205 1.9211632 5.169486 95-97
CTCTA 3108075 1.9154681 8.069642 25-29
GCTGG 2697445 1.9040117 10.168807 4
CTTGG 3451785 1.901528 7.289421 2
CGGAA 2301065 1.8982782 5.9695435 95-97
TGCTG 3432660 1.8909925 12.580564 6
CCTGT 2926335 1.888337 9.179647 1
CCAAT 2502085 1.8869982 20.107973 3
CTTTC 3736950 1.881979 6.2073097 7
GCTTC 2912740 1.8795645 6.722338 15-19
AATGA 3526680 1.8512734 6.2108088 75-79
CCTCC 1616950 1.834438 6.7972245 70-74
CTGTA 3460705 1.8207552 7.4920926 8
CACAA 1970350 1.8184394 5.4130945 85-89
GCAGG 2099085 1.8131471 5.577903 4
TTCCA 2917430 1.7979761 5.4359245 95-97
TAGCT 3408895 1.7934968 5.8927493 2
TTCCT 3546815 1.7862245 5.404509 20-24
CTGTT 4145070 1.7821034 10.062115 8
GGAGA 2527895 1.7803016 5.6988277 7
GAAAA 2761615 1.7739999 5.6876564 40-44
TTCGG 3216645 1.7719936 11.113774 7
CCTTA 2870050 1.7687762 5.423868 95-97
TCCGT 2740475 1.7684033 7.1194544 95-97
TGGTA 3908310 1.7554139 8.695113 2
TACTG 3326570 1.7501838 11.976854 3
CATCC 1873410 1.7328892 5.488824 90-94
TGGCT 3141705 1.7307105 6.3307233 9
GGCTC 2081030 1.7206473 5.240307 1
ATGAT 3994475 1.7134765 5.778355 15-19
TCGGT 3102445 1.7090828 13.051757 8
CACCC 1229840 1.7074229 5.600856 50-54
CTATT 4119550 1.6915263 5.3588185 25-29
TGCAA 2622030 1.6881484 6.250897 35-39
ACCAG 1736535 1.6780736 6.296309 30-34
CTACC 1809595 1.6738608 7.2143807 20-24
GCACC 1409015 1.6699811 5.053375 75-79
TATTC 4065995 1.6695361 6.3132195 5
TGTAG 3698950 1.6613799 6.45914 9
ATTGC 3133975 1.6488552 5.1439815 55-59
ATGCC 2075070 1.6386048 7.7398076 9
ATTCC 2650435 1.6334302 5.9600277 20-24
CTATG 3064345 1.6122214 5.521008 4
ACGGT 2387025 1.6091701 7.298123 4
CCGCC 1100215 1.5993444 9.070724 45-49
GCTGT 2882780 1.5880733 12.908766 7
TTGGT 4307585 1.5810238 10.617647 7
GTGGC 2227670 1.5724176 7.5328455 8
TACTT 3826290 1.571111 5.7074847 5
TTCAT 3821770 1.569255 5.972003 60-64
CACTG 1978040 1.5619838 5.2425137 35-39
TTGCA 2939435 1.5465033 5.276822 65-69
CGCAG 1526230 1.5442566 6.0077314 90-94
GACGG 1765295 1.524826 9.6302185 3
GGTAC 2246035 1.5141243 9.603224 3
GCTTG 2691665 1.4827913 6.960902 1
ACACA 1590645 1.4680089 5.128953 85-89
TCCTG 2273560 1.4671074 6.4784513 75-79
GTAGC 2174350 1.4657992 7.220035 1
AAACC 1578800 1.4570773 5.2925863 40-44
GCACT 1844135 1.456244 6.0461726 35-39
GTGTT 3902065 1.4321848 7.948146 9
CTGAC 1812270 1.4310815 8.210381 1
TGTTT 4956725 1.4198463 7.5091596 9
GGTCA 2096215 1.4131259 16.264763 9
GTGTA 3140240 1.4104359 9.637375 2
AAAAC 1873090 1.4094368 5.070704 40-44
GGCTG 1994260 1.4076633 6.4525194 9
ACTCT 2275915 1.4026183 5.61124 25-29
AGCCT 1769145 1.3970273 8.500215 2
AACCA 1505485 1.3894147 5.1023383 75-79
AACAC 1501225 1.3854831 5.1371894 85-89
ACTTT 3345390 1.3736489 5.5838256 6
TCTAT 3326065 1.3657137 5.237225 25-29
CACAC 1205035 1.3640292 5.095414 95-97
GTATT 3860960 1.3534051 5.756925 4
TCCTC 1785680 1.349757 6.2628136 70-74
GCGGC 1267765 1.343106 8.448463 7
CAATT 2656285 1.3347182 13.326773 4
ACATG 2066775 1.3306571 9.482869 1
TGGTC 2399095 1.3216196 13.438594 8
ATGGG 2284880 1.3149582 10.117814 3
CAGGA 1551100 1.2795898 5.2543883 5
ACCGC 1078115 1.2777947 5.354123 45-49
AGGTA 2318375 1.2742662 6.467446 6
TGACG 1885255 1.270911 7.147087 2
GTACT 2412490 1.2692657 7.239386 4
GTATG 2822510 1.2677279 5.067048 7
GCCTG 1525810 1.2615775 8.606731 6
AAGCC 1283550 1.2403387 9.026468 1
GTCTG 2236760 1.232192 5.064359 90-94
TGTAT 3501995 1.227575 5.604613 3
TACCA 1625490 1.2258962 5.3628335 30-34
GGTCG 1728890 1.2203501 8.707509 6
TACCC 1279710 1.1837214 5.560365 20-24
TACTC 1912450 1.1786193 5.2737365 25-29
GTAGA 2137345 1.1747653 5.855995 4
GCGAC 1144895 1.1584176 6.558519 20-24
ATTGG 2566385 1.1526895 11.494445 6
TGGGT 2433765 1.144567 6.4681225 4
TATGG 2528065 1.1354781 5.064163 5
TCCTA 1783205 1.0989671 5.5446525 20-24
GTTCG 1984255 1.0930915 10.926118 4
CGGTA 1618180 1.090867 6.7253623 5
GGGTC 1538695 1.0860994 9.811252 9
TGGCC 1308190 1.081644 9.804744 1
ATTCG 2006560 1.0556966 7.9754643 6
CGCCT 1084880 1.0507312 5.847061 45-49
CCAGG 1030480 1.0426512 5.4642577 1
TGCGG 1441225 1.0172994 6.0016017 6
TCGTG 1841060 1.0142078 6.687105 8
TTGGC 1838480 1.0127866 5.869526 3
AATTG 2347930 1.0071721 10.411375 5
TGGGG 1665815 1.0038004 7.6002226 7
CGGCT 1186265 0.98083335 6.3959103 8
CGACT 1173115 0.9263648 5.0026774 20-24
GCCCA 759675 0.9003757 6.3402295 2
GGGGT 1486435 0.895708 7.46262 8
GTCGC 1029950 0.85158825 5.0331197 10-14
GGCCC 642100 0.7968392 8.66748 1
GCCTA 1006715 0.79496497 8.123781 3
GGCCT 920305 0.760931 8.43352 5
CCCTA 814980 0.7538499 6.200161 2
GCCCT 764605 0.7405375 6.989342 1
GTCGT 1270720 0.7000175 6.5347953 7
CCTAT 1073735 0.6617296 6.0771565 4
GGTAA 1110435 0.61033696 5.643618 7
CCTAG 523545 0.4134238 5.1249437 3
Produced by FastQC (version 0.10.1)