FastQCFastQC Report
Mon 21 Jul 2014
20L22A_TGACCA_L002_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 20L22A_TGACCA_L002_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 18745942
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[WARN] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 391239 2.0870596953730036 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 170590 0.9100102838256942 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 161977 0.864064339898203 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 148887 0.7942358938270481 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 145864 0.7781097370300196 No Hit
GACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCT 126468 0.6746420105215305 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 123062 0.6564727448745974 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 98501 0.5254523885756182 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 93199 0.497168933948478 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 86365 0.46071304392171913 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 82307 0.4390656921908752 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 77594 0.4139242509125442 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 77079 0.4111769896652833 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 76197 0.40647197137385793 No Hit
TGACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTC 73299 0.3910126255591744 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 69122 0.3687304697731381 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 68880 0.36743952371131844 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 64567 0.34443187757649096 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 62793 0.3349684961150525 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 61729 0.329292601033333 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 61581 0.32850309683023665 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 59355 0.31662852685663917 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 55788 0.29760040866444587 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 55056 0.2936955635518343 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 51838 0.2765291816223479 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 50479 0.2692796126222945 No Hit
ACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGACTG 49788 0.26559348151189205 No Hit
TACAACATCGTAGCTGCTCACGGTTACTTTGGTAGATTAATTTTCCAATA 48549 0.25898405105488964 No Hit
TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC 44561 0.23771011347415885 No Hit
ACCCAATTTGGGAAGCTGCTTCCGTTGATGAATGGCTTTACAACGGTGGT 43018 0.2294789987080937 No Hit
GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA 41962 0.22384577952924428 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 41774 0.2228428958117976 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 40573 0.216436175893428 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 39993 0.21334217293534782 No Hit
GTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATATTGATGGTAT 39797 0.21229661331503108 No Hit
GCTAAAAACTATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGA 37804 0.2016649790125244 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 37582 0.20048072270787992 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 36243 0.19333784346500163 No Hit
CTGCTGCTATCATCCCTACTTCTGCAGCTATCGGTTTGCACTTCTACCCA 35204 0.18779531057975107 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 34365 0.18331967526625229 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 34297 0.18295693009185668 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 33921 0.1809511626569633 No Hit
CATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGC 33882 0.1807431176304717 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 33712 0.1798362546944827 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 32898 0.17549398157745288 No Hit
ATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTG 32098 0.17122639129044567 No Hit
CCACGTAGTTGGTTAGCTACTTCTCACTTTGTATTAGGATTTTTCTTCTT 30122 0.16068544328153794 No Hit
ATACACTTGGGTAACTTATTATTATTTTACAAACCAAGATTTACCATGAC 30103 0.16058408801222152 No Hit
TACCATGACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTC 28764 0.15344120876934325 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 28618 0.15266237354196444 No Hit
GCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATG 27606 0.14726387182890036 No Hit
TTCATGCGTTGGAGAGACCGTTTCTTATTCTGTGCAGAAGCTATTTACAA 26764 0.1427722330518253 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 26573 0.14175334587080232 No Hit
ACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGACGG 26068 0.13905942950212904 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 25333 0.1351385809259412 No Hit
ACTCTATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCC 23782 0.12686479025700603 No Hit
GCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCT 23443 0.12505639887288672 No Hit
TACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAAC 23159 0.12354140432099918 No Hit
CTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGT 22992 0.12265054484858642 No Hit
GCGACTGGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGT 22873 0.12201574079339411 No Hit
GCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGT 22443 0.11972191101412774 No Hit
GCTGCTATCATCCCTACTTCTGCAGCTATCGGTTTGCACTTCTACCCAAT 22291 0.11891106885959639 No Hit
ATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAA 22134 0.11807355426577122 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 21962 0.11715602235406468 No Hit
ATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCA 21855 0.11658523215317747 No Hit
GTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAG 21745 0.115998438488714 No Hit
ATGCACCCATTCCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCT 20996 0.11200290708250352 No Hit
GCTCATGGTAAGGCTTTATATGGTTTTGATGTTCTTTTATCATCTGCAGA 19955 0.10644970522153542 No Hit
TATACACTTGGGTAACTTATTATTATTTTACAAACCAAGATTTACCATGA 19870 0.10599627375354091 No Hit
CCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGATT 19732 0.10526011442903217 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 19417 0.10357975075352309 No Hit
ACACTTGGGTAACTTATTATTATTTTACAAACCAAGATTTACCATGACTG 19328 0.10310498133409354 No Hit
CAGCTCCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGT 19324 0.10308364338265849 No Hit
GCACCCATTCCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTAT 18877 0.10069912730979323 No Hit
TACTTGGTGTAGCTTGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGT 18858 0.1005977720404768 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 9340050 5.140871 7.527025 70-74
CTGCT 6517430 3.781029 13.469566 4
ACTTC 6730780 3.641201 7.5237093 60-64
AGCTG 5531325 3.4711328 7.8405833 3
AACTT 7289065 3.2408915 6.4229565 60-64
CTACT 5683395 3.0745893 12.818766 2
CAAGC 3395390 2.9669127 9.781066 75-79
CTTCT 6592905 2.9061322 5.8476205 20-24
TGCTA 6093610 2.9055052 8.272868 4
GCTGC 3761275 2.8718634 11.248886 3
ACCCA 2894740 2.8698354 46.82929 1
ACAAC 3514410 2.8636057 9.473867 85-89
TCAAG 4879265 2.8552368 6.061024 10-14
CAACT 4285630 2.8453434 9.42339 9
CTGGT 5490945 2.8076854 11.126207 1
CTTCA 5174795 2.799448 7.3964167 60-64
GGAAG 4057955 2.7545948 7.3733425 15-19
AGGAA 4277235 2.7074425 7.364516 40-44
CGGTG 4002755 2.693738 12.05817 9
TTCAA 6058325 2.6936748 7.691237 55-59
TCTCT 6085085 2.6822867 9.199623 20-24
GCTAC 3756285 2.6744401 17.16015 1
GGTGT 5859490 2.6407595 10.565225 9
TGGTG 5811065 2.618935 9.284922 8
AGCAC 2985810 2.6090193 5.4310675 65-69
ACTGC 3640460 2.5919738 11.980833 2
TATGC 5415555 2.5822005 9.062302 8
GGTGG 4299590 2.5502994 7.099101 7
ACATC 3814465 2.5325243 7.1556034 90-94
AAGCT 4322030 2.5291553 6.2496495 75-79
TCAAC 3740645 2.4835134 7.6314874 55-59
CAACA 3036220 2.4739673 8.737751 85-89
GCAGC 2621445 2.4564629 8.006662 1
CAGCA 2797685 2.4446344 6.5635195 60-64
CTGAA 4170575 2.4405272 6.5867395 80-84
GAAGC 3135045 2.4144967 8.251753 15-19
CCAAG 2753830 2.4063137 8.625066 75-79
ATGCA 4076715 2.3856025 6.055142 1
ATGGT 5471595 2.2994745 5.3572807 1
TGAAA 4776840 2.2974274 5.5923758 70-74
CAGCT 3226730 2.297402 7.168336 2
CATGG 3657525 2.2952466 14.623125 2
CTTTA 6327750 2.292457 7.407231 5
CTTGG 4424040 2.2621448 9.149001 6
CGTGA 3601415 2.2600353 12.282211 9
TCTGC 3893050 2.2585182 5.4828815 85-89
TCCTT 5103920 2.249792 6.3797 40-44
CCGTG 2933950 2.2401721 5.9648724 60-64
TAGCT 4693575 2.237952 5.731941 2
CAAGG 2878735 2.2170959 7.8454857 10-14
GCTGG 3244445 2.1834173 8.673193 4
CCAGC 2031690 2.1600273 6.7420955 1
GTTCC 3657275 2.1217356 5.4388223 70-74
GCTTC 3644495 2.1143212 8.539438 15-19
CCTTA 3907355 2.1137915 8.67319 95-97
CTCCT 3195100 2.1030538 7.496671 70-74
CTCTG 3602795 2.0901294 8.502371 20-24
GCTCC 2402445 2.081203 6.0687947 70-74
CTGCA 2898430 2.0636556 5.073592 95-97
CTCTC 3110650 2.0474677 5.0860047 15-19
CACAA 2479170 2.0200732 7.89651 85-89
TTCCA 3684210 1.993075 5.988481 75-79
CCCAA 1985620 1.9685367 46.759827 2
AAGGA 3108750 1.9678046 6.949335 10-14
AGCTC 2723990 1.9394556 5.036373 70-74
TTCGG 3792445 1.939191 9.180018 7
GAAAG 3059355 1.9365381 5.277666 7
TCGGT 3785055 1.9354124 11.303617 8
TATTC 5312575 1.924673 6.080953 5
GGTAT 4531310 1.9043134 7.6474533 6
CTATG 3980710 1.8980495 5.8149385 35-39
CCGCC 1464205 1.8940449 6.6753483 75-79
CATCC 2327900 1.8804898 7.901375 90-94
ACCAG 2150545 1.8791597 5.260376 60-64
CCAAT 2818610 1.8713499 31.553534 3
CCACA 1876810 1.8606629 9.908399 1
TTTCA 5132940 1.8595937 6.4883914 8
CACCC 1533520 1.8497947 6.8586264 50-54
CCTGT 3181415 1.8456694 7.6645527 1
GTGTA 4375460 1.8388164 9.494566 2
GCCTT 3167700 1.8377128 5.4229007 35-39
TTGGT 5358875 1.8350486 18.769558 7
ATCGG 2891115 1.814293 5.41354 85-89
TGTAG 4314645 1.8132585 5.0895386 3
GCACC 1700395 1.8078051 6.2090473 75-79
GTGGC 2685170 1.8070418 5.9259567 8
CTCTA 3322410 1.7973493 5.095492 95-97
GTAGC 2850340 1.788705 6.559645 1
TGCTT 4581830 1.7801032 5.9949536 4
ACGGT 2824305 1.7723671 11.661363 4
TCTGG 3462625 1.7705443 5.2400556 45-49
GCTTG 3433910 1.7558614 6.7419825 5
CATGC 2461270 1.7524015 8.86987 2
TCGGA 2786835 1.7488532 5.6430387 85-89
TACTT 4799155 1.738668 8.791661 3
GCACT 2428910 1.7293614 5.02939 35-39
GCAAG 2231580 1.718681 5.698771 25-29
AGAAA 2910990 1.7182349 9.156936 9
CTTTC 3897865 1.7181668 7.648014 7
TGGTA 4085495 1.7169567 8.718689 2
ACACA 2093255 1.7056223 7.6050034 85-89
AGCTT 3532275 1.6842303 5.0629163 15-19
GAACA 2304760 1.6552142 6.9297776 80-84
TCCAA 2491040 1.6538675 6.440944 75-79
AACAC 2007275 1.6355644 7.6910014 85-89
TTTAG 5098570 1.6280509 6.1325445 6
AATGA 3371400 1.6214792 5.1478124 75-79
ACATG 2760215 1.6152161 16.287655 1
TTAGG 3837385 1.6126869 6.044046 35-39
AGCCT 2264255 1.6121286 5.8200216 2
CTACC 1988155 1.606042 5.3262224 25-29
GGTCG 2379050 1.6010318 12.78069 6
GCTGA 2535475 1.5911143 6.2137065 80-84
ATGGG 2867755 1.5861775 12.283464 3
ATGCC 2212520 1.5752938 12.431722 9
CTGTA 3280185 1.5640309 6.760886 8
CATGA 2664730 1.5593404 5.528515 65-69
AAGCC 1783300 1.5582587 7.2541947 2
GACGG 1871410 1.545632 15.290062 3
ACTTT 4260880 1.5436584 9.079967 4
ACCGC 1451810 1.5435175 5.328171 75-79
TGAAC 2616395 1.5310558 6.2568426 80-84
GGTAC 2429570 1.5246546 10.863122 3
GCAAC 1740245 1.5206369 8.826111 1
AACAT 2770520 1.5118012 5.401879 90-94
AAAGC 2074920 1.4901493 5.175411 20-24
CTGCG 1919320 1.4654672 5.2621574 45-49
CGCAG 1560295 1.4620969 5.383863 90-94
GGGTC 2166760 1.4581668 12.684089 5
GCGGC 1445040 1.4521239 9.297656 7
ATTCC 2658185 1.4380184 5.3570046 80-84
GACTG 2271510 1.4254655 9.539363 1
GGCTG 2110685 1.4204298 6.9379845 9
CTGAC 1991645 1.418033 12.97534 1
ATGCT 2960340 1.411525 6.1592145 3
GGAAT 2714065 1.3998313 5.160557 40-44
GTATT 4367655 1.3946586 5.964222 4
GTCAA 2370320 1.3870583 5.673495 10-14
GGTCA 2201185 1.3813338 26.429321 9
TGGTC 2700590 1.3808928 21.837538 8
TGGGT 3054790 1.3767351 9.072247 4
ATCTC 2542545 1.3754598 5.5655513 45-49
GAATC 2295965 1.3435473 5.538816 40-44
GTATG 3099225 1.3024701 7.753245 7
AACCG 1487710 1.2999703 5.5838823 70-74
CAATT 2919525 1.2980901 21.496069 4
CACAT 1950515 1.2949988 6.7098603 2
TGTAT 3970120 1.2677196 5.7652392 3
TGCCT 2167240 1.2573049 5.3651357 30-34
TGACG 1986720 1.2467481 11.446884 2
GTACT 2613465 1.246131 8.286279 4
GCGCA 1327050 1.2435313 5.223189 25-29
GCCTG 1621420 1.2380102 5.166045 6
TGGGG 2074210 1.2303165 5.15519 8
ATTCG 2572445 1.226572 7.589821 6
ATCCT 2249150 1.2167397 5.179608 90-94
AAGAC 1688975 1.2129744 5.197659 9
GACGC 1294130 1.2126832 5.212486 15-19
AACGC 1358455 1.1870264 5.67202 95-97
TGCGG 1668135 1.1226065 6.533614 6
CGGTA 1786750 1.1212587 11.096538 5
ATTGG 2651155 1.1141657 18.471155 6
CTTAT 3069500 1.1120377 5.559976 95-97
GCTCA 1553310 1.1059424 5.893322 1
TGGCC 1401210 1.0698723 7.6659226 1
TCGTG 2065365 1.0560832 9.86565 8
CGGCT 1370150 1.0461569 6.919674 8
GCCTA 1464230 1.0425181 5.6501093 3
TAGAA 2143325 1.0308349 7.238006 8
TTATG 3109285 0.99284196 5.0442843 95-97
AATTG 2495075 0.97778636 16.737526 5
ACGCG 1026530 0.96192473 5.848869 15-19
TTAGA 2127150 0.8336015 6.395935 7
CACGG 879540 0.8241857 6.092935 4
CTACA 1211315 0.80422413 7.5829964 3
GTCGT 1521330 0.7779017 9.618425 7
GGCCT 994795 0.75956035 5.025712 5
GGCCC 592340 0.67534727 5.045897 1
Produced by FastQC (version 0.10.1)