FastQCFastQC Report
Mon 21 Jul 2014
20L15A_ACTTGA_L002_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 20L15A_ACTTGA_L002_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 14891175
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 371806 2.496821103774551 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 225526 1.5144943229798857 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 147005 0.9871954362231322 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 123900 0.8320364242579916 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 105143 0.7060759140900567 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 86783 0.5827814124808821 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 77752 0.5221347543091798 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 72871 0.48935695134870144 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 70401 0.4727699459579247 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 66944 0.4495548537976352 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 64408 0.432524632878198 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 61181 0.41085407968142207 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 57218 0.3842410018014025 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 56730 0.3809638930440345 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 56507 0.37946636178810605 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 56371 0.3785530691835936 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 51217 0.3439419656272927 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 51041 0.34276005755086486 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 48522 0.3258439982069917 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 45758 0.3072826690976367 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 42264 0.28381910762582535 No Hit
TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC 42114 0.28281179960614256 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 41629 0.27955483700916817 No Hit
GACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCT 40432 0.27151651901209944 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 38544 0.25883786873769193 No Hit
GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA 37971 0.25498995210250364 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 36549 0.24544067207591075 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 35668 0.23952441630697377 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 35250 0.23671738462545772 No Hit
ACCCAATTTGGGAAGCTGCTTCCGTTGATGAATGGCTTTACAACGGTGGT 34703 0.2330440680470144 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 34391 0.2309488673660742 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 33904 0.22767847399550403 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 33132 0.22249419538753656 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 30416 0.20425520484448004 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 30042 0.2017436501820709 No Hit
TACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAAC 28557 0.19177130078721122 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 28536 0.19163027766445564 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 27904 0.1873861532081921 No Hit
GCTCATGGTAAGGCTTTATATGGTTTTGATGTTCTTTTATCATCTGCAGA 27561 0.18508277553651742 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 26863 0.18039543555159349 No Hit
GCTAAAAACTATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGA 25828 0.17344501021578218 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 25767 0.17303537162111116 No Hit
CCACGTAGTTGGTTAGCTACTTCTCACTTTGTATTAGGATTTTTCTTCTT 25471 0.1710476171289371 No Hit
ACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGACTG 23772 0.15963817495932994 No Hit
TTCATGCGTTGGAGAGACCGTTTCTTATTCTGTGCAGAAGCTATTTACAA 23183 0.1556828121353755 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 23150 0.15546120437104527 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 23002 0.15446732712495823 No Hit
CTGCTGCTATCATCCCTACTTCTGCAGCTATCGGTTTGCACTTCTACCCA 22789 0.15303694973700868 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 21984 0.147631063364711 No Hit
TACAACATCGTAGCTGCTCACGGTTACTTTGGTAGATTAATTTTCCAATA 21376 0.1435481081915967 No Hit
TGACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTC 21080 0.14156035369942266 No Hit
CCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCCAGAA 20674 0.1388339066594812 No Hit
CAAGGTGAAACAGGTGAAATTAAAGGGCATTATCTAAATGCTACTGCAGG 19658 0.13201107367282972 No Hit
CATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGC 19291 0.1295465267180058 No Hit
CCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGATT 19216 0.1290428727081644 No Hit
GTATGGGCTTGGATGTTCTTATTTGGACATCTTGTTTGGGCTACTGGGTT 19092 0.12821016474522662 No Hit
ACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGACGG 19035 0.12782738769774715 No Hit
GCGACTGGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGT 18961 0.12733044907470364 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 18101 0.12155521642852225 No Hit
CAGCTCCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGT 17537 0.11776773827451494 No Hit
GCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGT 17000 0.11416157556405052 No Hit
GCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCT 16616 0.11158286703366255 No Hit
ATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGGAT 16417 0.11024650506088338 No Hit
GTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAG 15795 0.1060695344725987 No Hit
CTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGT 15497 0.10406834920682888 No Hit
ATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGATTTCACTAAA 15302 0.10275884878124122 No Hit
ATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTG 15274 0.10257081795090044 No Hit
ACCTCCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCC 15240 0.10234249479977235 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 6078020 4.3952017 6.424546 90-94
CTGCT 4916800 3.5832193 10.766722 4
ACTTC 5060715 3.569078 8.186025 60-64
AACTT 5686615 3.3452764 7.242507 60-64
AGCTG 4070910 3.104313 7.7624454 3
CAACT 3494995 2.9922154 9.748128 55-59
CTACT 4192985 2.9571095 10.437436 2
TTCAA 4885225 2.873841 8.854148 55-59
CTTCA 4040775 2.8497636 8.265578 60-64
TCAAG 3790650 2.7973115 6.9153147 10-14
TCAAC 3247120 2.779999 9.491464 55-59
AGGAA 3582630 2.7663822 8.1540365 40-44
ACCCA 2216105 2.7612436 54.50104 1
GCTGC 3017710 2.7587817 11.219904 3
TGCTA 4443480 2.7011468 6.461793 1
ACAAC 2582395 2.6839297 10.145812 85-89
CTTCT 4618755 2.683285 6.675063 20-24
CAAGC 2497695 2.682469 10.293959 75-79
GGAAG 3348560 2.6718688 8.032974 15-19
TCTCT 4553640 2.6454563 9.942724 20-24
GCTAC 2932240 2.594135 13.803519 1
CTGGT 4120770 2.5885117 10.252254 1
ACTGC 2916235 2.5799756 7.069935 4
AAGCT 3490920 2.5761256 7.1849947 15-19
TATGC 4100020 2.492361 8.846021 30-34
GAAGC 2684860 2.4854085 9.120777 15-19
CAACA 2299840 2.3902655 9.723376 85-89
CAGCA 2196105 2.358568 7.150359 2
AGCAC 2192775 2.3549917 5.58539 35-39
CCAAG 2186040 2.3477583 9.737343 75-79
ACATC 2740875 2.3465807 7.893431 90-94
CGGTG 2974655 2.3439987 14.1258955 9
GGTGT 4306570 2.3317616 12.079304 1
GCAGC 2099095 2.3295617 9.340856 1
CAAGG 2501695 2.3158505 8.619971 10-14
TGGTG 4275935 2.3151746 7.3726864 6
CTGAA 3106200 2.2922213 7.295994 80-84
TCCTT 3933530 2.2852006 8.195979 40-44
CAGCT 2577605 2.2803917 7.7551265 65-69
GGTGG 3354210 2.2782001 7.2944026 7
CATGG 2931065 2.2351127 21.192905 2
TGAAA 3535650 2.176359 5.3645844 65-69
CTCTG 2950980 2.1505876 9.3246355 20-24
CTGCA 2418135 2.1393094 5.5519853 95-97
GCTCC 1999640 2.120854 7.987052 65-69
CTTGG 3352865 2.106143 6.924429 2
CTCCT 2468085 2.0867486 9.666753 70-74
CGTGA 2734670 2.0853496 18.958899 9
AAGGA 2676430 2.066646 7.849976 10-14
CCCAA 1652330 2.0587857 54.98626 2
ATGGT 3922530 2.0552855 5.6277585 1
CACAA 1972855 2.050424 9.233493 85-89
TCTGC 2774160 2.0217264 5.3765683 85-89
GTTCC 2773095 2.0209503 6.264184 70-74
GCTTC 2769780 2.0185344 9.790561 15-19
CCTTA 2853975 2.0127707 10.220886 95-97
CTTTA 4150005 2.0110583 5.4211864 35-39
TAGCT 3295100 2.0030582 5.639999 2
TTTCA 4124955 1.998919 5.8787727 8
AGCTC 2249470 1.9900925 7.0182433 65-69
TTCCA 2795255 1.9713582 6.9596224 75-79
GGTAT 3738175 1.958689 7.2025967 6
CCAGC 1520775 1.9580607 7.9023747 1
GCTGG 2466595 1.9436523 8.635777 4
CTCTC 2289315 1.9355997 5.4350743 15-19
CCGTG 2088400 1.9092094 5.6746497 60-64
CCAAT 2226520 1.9062195 37.859135 3
CATCC 1830435 1.8787392 9.0573635 90-94
TATTC 3871750 1.8762182 7.8628583 5
TGGCT 2979330 1.8715024 5.1847057 9
TGCTG 2956835 1.8573718 5.1603203 6
CTTTC 3123260 1.8144711 6.9455156 7
TACTG 2971360 1.8062601 5.078636 85-89
CTCTA 2530705 1.7847838 5.0387535 25-29
CACCC 1194635 1.7844957 6.4212418 75-79
CCTGT 2445415 1.7821466 7.4663653 1
GGCTC 1936130 1.7700045 5.0563254 10-14
ACGGT 2300390 1.7541852 10.751529 4
GCCTT 2406305 1.7536446 6.317494 35-39
ACACA 1683260 1.7494427 8.968135 85-89
CTACC 1692715 1.7373849 7.3388357 25-29
GCTGT 2760265 1.733894 5.2952843 7
GAACA 1933760 1.7323352 8.228839 80-84
TCTGG 2751860 1.7286143 5.8226185 45-49
TTCGG 2748150 1.7262837 11.203167 7
GCACC 1332990 1.7162797 5.9386272 75-79
TCGGT 2727450 1.7132809 13.566138 8
CCACA 1361960 1.6969879 7.6739783 1
TTGGT 3921325 1.6925304 20.183435 7
GCAGG 1767865 1.6911107 5.191893 90-94
GTTGC 2682850 1.6852647 5.717 75-79
TTAGG 3211880 1.6829265 6.901523 35-39
ACCAG 1563500 1.6791643 5.170901 30-34
TTCAG 2754330 1.6743296 5.5398703 65-69
TCCAA 1947900 1.6676809 7.6407156 75-79
AGCTT 2741960 1.66681 5.8323555 15-19
AATGA 2697505 1.6604412 6.029662 75-79
AACAC 1587290 1.6496991 9.007354 85-89
TACTT 3380215 1.6380243 7.3279986 3
TGTAG 3125730 1.6377866 6.044193 9
GTGGC 2056365 1.6203952 6.080104 8
ACATG 2179125 1.6080861 21.499735 1
GTGTA 3063300 1.6050752 11.464857 2
CCGCC 1036440 1.5998206 7.11319 45-49
GCTGA 2097500 1.5994693 6.972495 80-84
GCACT 1805555 1.5973636 5.35119 35-39
ATGCC 1801705 1.5939577 11.6863 9
TGGTA 3036090 1.590818 7.79036 2
GCTTG 2523660 1.5852677 6.91529 1
TTTAG 3791730 1.5837779 5.4054728 35-39
GCGGC 1368975 1.5699447 8.838126 7
CCTTT 2699035 1.5680156 5.27249 35-39
CATGC 1754120 1.5518595 6.3946786 2
GACGG 1609600 1.5397171 13.65412 3
GCAAC 1422860 1.52812 6.6458697 1
AGAAA 2043875 1.5272769 5.9016123 9
TGAAC 2064935 1.5238196 6.995215 80-84
GGTAC 1990800 1.5181041 9.327803 3
CATGA 2054390 1.5160381 6.4481 65-69
GTAGC 1983620 1.5126289 6.54311 1
CTGTA 2482855 1.5093026 7.198855 8
GATCG 1964880 1.4983386 6.1203876 50-54
AACAT 2095595 1.4965379 6.3038173 90-94
GCAAG 1615980 1.495933 5.6002345 1
GGCTG 1887570 1.4873865 6.837872 9
GGGAA 1863825 1.4871753 5.56254 15-19
TCAGC 1672490 1.4796419 5.7127776 65-69
TTCAT 3045835 1.4759864 5.920033 60-64
AATCT 2489470 1.4644855 5.2806253 40-44
ATTCC 2067850 1.4583548 5.836516 80-84
ACTAC 1702225 1.4573481 5.09084 70-74
ACTTT 3004870 1.4561353 7.621279 4
GAATC 1972415 1.4555444 6.481773 40-44
GTCAA 1965405 1.4503714 6.5544434 10-14
CGCAG 1304745 1.4479973 5.934074 90-94
GGAAC 1560930 1.4449725 6.2262993 15-19
ATGGG 2196215 1.443543 18.222776 3
GGAAT 2265035 1.4407314 5.833058 40-44
AACCA 1371740 1.4256743 5.1670136 75-79
GTGTT 3300695 1.4246529 5.2064786 70-74
GGTCG 1804230 1.4217153 19.328182 6
TCATG 2328340 1.4153746 5.449441 60-64
ATCTC 1992190 1.4049954 6.624129 45-49
AGGTA 2204655 1.4023249 6.1700993 35-39
TGGGA 2118885 1.3927149 5.8210864 15-19
CAATT 2338635 1.3757535 26.135824 4
GTATG 2598360 1.3614609 7.4872065 7
TGGTC 2162395 1.3583347 25.16014 8
GGTCA 1779885 1.3572688 30.220413 9
CTGAC 1518750 1.343629 12.228921 1
AGCTA 1814230 1.3388116 5.3374033 20-24
TGCCT 1808480 1.317967 6.248821 30-34
GTATT 3145260 1.313752 7.1926146 4
AAGCC 1220805 1.3111174 7.830639 1
TTCTC 2256040 1.3106558 5.9498796 20-24
ACCGC 1009975 1.3003846 5.3131466 45-49
GGGTC 1638705 1.2912832 19.2594 5
GTACT 2124020 1.2911704 7.3751183 4
TGACG 1684485 1.2845206 10.648778 2
TGGGT 2358825 1.277169 13.820866 4
AGCCT 1440430 1.2743397 6.285705 2
ATCCT 1770630 1.2487397 6.1500645 90-94
CAGGT 1631385 1.2440288 5.3827553 95-97
GTGAG 1877085 1.2337829 5.742367 10-14
CACAT 1407415 1.2049484 5.0004683 2
TAGGA 1892255 1.2036154 5.4839773 40-44
CGCTG 1311515 1.1989833 6.1582513 50-54
TGTAT 2859830 1.19453 7.083436 3
TGCGG 1514595 1.1934857 6.393815 6
TCTGA 1962735 1.1931269 5.333219 20-24
AACGC 1103930 1.1855962 5.1507115 90-94
GCGAC 1054230 1.1699774 5.3395343 20-24
GCCTG 1276260 1.1667533 8.005732 6
TCGTG 1842460 1.1573637 15.452067 8
ATTCG 1890525 1.1492313 9.668606 6
ATTGG 2166885 1.1353813 21.703585 6
CGGTA 1483845 1.1315207 10.210143 5
CCTTG 1550625 1.13005 5.8353305 45-49
CGGCT 1234835 1.1288826 6.848206 8
GTGGG 1652030 1.122069 5.328668 10-14
TGGCC 1223350 1.118383 10.712981 1
ACCGT 1228790 1.0871031 5.553869 25-29
CTTAT 2239390 1.0851899 6.859604 95-97
TACAT 1827655 1.0751584 5.7443767 4
GCTCA 1198725 1.0605047 6.337929 1
AATTG 2065570 1.0473672 20.327688 5
GGATC 1369135 1.0440475 5.280879 45-49
TGGGG 1533175 1.0413419 5.7792645 8
CGTCC 972555 1.0315092 6.51677 40-44
TCTTG 2047230 1.0251547 5.349587 95-97
TTGGC 1577045 0.99063987 5.4725585 3
TTATG 2341690 0.9781068 6.049848 95-97
ATGCG 1250050 0.953238 5.0102034 35-39
GGGGT 1386340 0.94161063 5.22558 9
TACCG 1061355 0.93897444 5.4531326 25-29
GCGTC 1017530 0.93022305 5.5632896 40-44
ATCGC 1047300 0.9265401 5.4585733 50-54
GTCGT 1346180 0.8456193 15.265637 7
CTACA 985995 0.84415275 8.617087 3
GGCCT 909725 0.831668 7.5903273 5
GCCTA 919935 0.81386095 6.1582527 3
GCATA 1070665 0.79009765 5.5531282 60-64
CACGG 679125 0.7536884 5.0003 4
GGCCC 551230 0.73339987 6.445476 1