FastQCFastQC Report
Mon 21 Jul 2014
1S15C_GATCAG_L004_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 1S15C_GATCAG_L004_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 13579132
Filtered Sequences 0
Sequence length 101
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[WARN] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 226952 1.6713292130896142 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 151484 1.1155646767407519 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 85936 0.6328534106598271 No Hit
GACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCT 85566 0.6301286415066883 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 84143 0.6196493266285356 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 65777 0.48439767725948907 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 64744 0.47679041635356373 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 62995 0.4639103589242671 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 59972 0.44164825851902756 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 57796 0.42562367020218966 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 52769 0.3886036309242741 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 51165 0.3767913884333697 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 47416 0.34918284909521463 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 43358 0.31929875930214097 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 42203 0.3107930609997753 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 42086 0.309931444808107 No Hit
TGACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTC 41255 0.3038117605749764 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 38635 0.2845174492743719 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 38007 0.27989270595499033 No Hit
GTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATATTGATGGTAT 31866 0.23466890225384068 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 30706 0.2261263827467028 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 29585 0.2178710686367877 No Hit
GCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATG 29435 0.2167664324936233 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 27648 0.20360653390805833 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 27194 0.20026316851474746 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 27041 0.19913643964871983 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 25837 0.19026989353958706 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 25479 0.18763349527790144 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 24491 0.18035762521492538 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 23263 0.17131433732288634 No Hit
ACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGACGG 23074 0.1699224957824992 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 22973 0.16917870744610186 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 21715 0.15991449232542992 No Hit
GTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGG 21561 0.15878039921844783 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 21322 0.15702034563033926 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 21137 0.15565796105376986 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 20398 0.15021578698844668 No Hit
CCTACTCTATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGC 20292 0.1494351774472772 No Hit
ATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTG 20010 0.14735846149812817 No Hit
ATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAA 19997 0.14726272636572058 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 19843 0.14612863325873848 No Hit
AACAAGTTTATACACTTGGGTAACTTATTATTATTTTACAAACCAAGATT 19801 0.14581933513865245 No Hit
ACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGACTG 19643 0.14465578506785262 No Hit
GGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCAT 18922 0.1393461673397092 No Hit
GCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGT 18840 0.13874229958144602 No Hit
TGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 18527 0.13643729216270967 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 18253 0.13441949014119606 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 17778 0.1309214756878422 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 17682 0.130214508556217 No Hit
TACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGA 17501 0.12888158094346533 No Hit
CCACGTAGTTGGTTAGCTACTTCTCACTTTGTATTAGGATTTTTCTTCTT 16690 0.12290918152942323 No Hit
ACTCTATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCC 16648 0.12259988340933721 No Hit
GCTAAAAACTATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGA 16436 0.12103866432699821 No Hit
TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC 16370 0.12055262442400588 No Hit
GCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAATG 16319 0.12017704813533 No Hit
CATCTTTTCAAGAGTTATTAATTGAGTTTTAGGGAGGGATTTATGTCACC 16249 0.11966155126851996 No Hit
GGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTATTT 16219 0.11944062403988707 No Hit
CTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGT 15690 0.11554494057499404 No Hit
ACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGAT 15593 0.11483060920241442 No Hit
CCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCCAGAA 14904 0.10975664718481269 No Hit
GGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTA 14853 0.10938107089613681 No Hit
ATACACTTGGGTAACTTATTATTATTTTACAAACCAAGATTTACCATGAC 14316 0.10542647350360833 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 14168 0.1043365658423528 No Hit
TGACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 14101 0.10384316169840604 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 14096 0.10380634049363391 No Hit
CATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGC 13993 0.1030478236753277 No Hit
CCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGATT 13774 0.10143505490630772 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 6279470 4.1187515 11.321485 70-74
CTGCT 4554275 3.7667978 11.229705 3
GGAAG 3669760 3.4821258 6.653115 15-19
AGCAC 3015510 3.4615645 7.282133 80-84
ACTTC 4581495 3.3504684 7.145639 60-64
AGCTG 3669125 3.250442 8.48358 1
GAAGA 3698270 3.1027777 6.1333284 95-97
AACTT 5188845 3.0504355 6.765315 7
CGGAA 2904470 3.0312803 7.9410763 95-97
CTGGT 4000830 3.008505 16.227833 1
CTTCT 4636470 2.878104 5.7748795 20-24
TGCTA 4272065 2.8404276 8.534733 4
CTGAA 3598935 2.8190267 6.9165072 80-84
CTACT 3845645 2.8123379 11.821759 2
TCTCT 4523110 2.8077354 8.269257 20-24
GGTGT 3987850 2.7263842 9.926591 1
TCAAG 3480055 2.7259085 5.5467215 10-14
CGGTG 2697340 2.702529 10.57926 9
ATCGG 3035470 2.689093 7.6509037 85-89
TGGTG 3930490 2.6871686 9.790352 4
TCGGA 2997565 2.6555135 7.9862194 85-89
AGAGC 2544065 2.65514 9.038904 95-97
CAAGC 2276145 2.6128325 8.51417 30-34
CTTCA 3566965 2.608538 7.2221704 60-64
TCTGC 3144355 2.6006663 6.3982263 85-89
ACAAC 2557555 2.5958638 8.048214 85-89
GGTGG 2818740 2.5676584 8.306942 7
GCTGC 2329690 2.5673506 8.474083 3
CCGTG 2300495 2.5351775 5.988302 60-64
TATGC 3756780 2.4978228 7.4386063 30-34
TTCAA 4227995 2.4855678 10.47723 9
CAACA 2444285 2.4808972 7.673588 85-89
AAGAG 2953510 2.4779387 5.4815392 90-94
CAACT 2857035 2.4614623 10.557955 9
ACATC 2837045 2.44424 6.0538683 90-94
GCAGC 1880810 2.4418068 5.7664905 1
AGATC 3083220 2.4150696 7.2130685 95-97
AGGAA 2875920 2.4128418 6.5286 40-44
GATCG 2706080 2.39729 6.903311 95-97
GAGCA 2290205 2.3901963 6.469128 90-94
ACCCA 1891545 2.3882556 35.501984 1
ATGCA 3011430 2.358837 6.731531 1
GCACA 2039605 2.3413036 7.017302 95-97
CGTGA 2628785 2.328815 5.8298087 9
GCTGG 2308650 2.313091 10.223498 2
CCAAG 1990520 2.2849581 7.5524426 75-79
TGAAA 3613650 2.2754335 5.876092 65-69
CAGCA 1977185 2.2696505 5.6732025 2
AAGCT 2890865 2.2643993 5.6384706 75-79
CACAC 1757640 2.2191875 7.7613835 95-97
GTTCC 2638475 2.182258 5.1548676 70-74
CATGG 2448790 2.1693592 9.095353 2
ACTGC 2226160 2.1691456 12.078177 2
GAAGC 2074230 2.1647916 7.356817 15-19
TCAAC 2477190 2.134209 8.854694 8
CTTTA 4202360 2.0970323 5.7676973 5
TCCTT 3375250 2.0951977 5.5661745 40-44
CTCTG 2494345 2.063049 7.0451074 20-24
GGCTC 1867450 2.0579557 5.961904 90-94
CAGCT 2109975 2.0559363 5.1118336 2
CTTGG 2702820 2.0324402 6.3993373 2
CCTTA 2737300 2.0018 7.4156165 95-97
GCTAC 2045010 1.9926351 14.179145 1
CCAGC 1394070 1.9906888 6.916732 1
TTCGG 2633385 1.9802272 7.9531417 7
TGGCT 2626120 1.9747641 6.1201015 9
TGAAC 2508090 1.9645736 6.507777 80-84
CAAGG 1876300 1.95822 7.0091577 10-14
CTCTC 2141470 1.948128 5.3231964 95-97
CATCC 1808305 1.938012 7.157955 90-94
TCGGT 2560530 1.9254423 8.818795 8
GTGGC 1913105 1.9167852 7.384834 8
TTCCA 2610895 1.9093596 5.770268 25-29
GCTTC 2296275 1.8992274 7.5697055 15-19
CCTGT 2268850 1.8765444 7.4797688 1
GTCTG 2448550 1.8412367 6.245445 90-94
TTTCA 3677880 1.8353101 9.191504 8
TCTGG 2437350 1.8328147 5.3010764 45-49
TTGGT 3562440 1.8279392 17.075182 7
AAGGA 2169575 1.8202319 6.075956 10-14
CACAA 1791050 1.8178777 6.628671 85-89
TATTC 3633110 1.812969 5.075893 5
GTGTA 2998805 1.8127657 8.682906 2
TGCTT 3203460 1.8079479 5.1480045 65-69
GCATG 2040570 1.807721 5.6115146 3
TGCTG 2401405 1.8057851 5.0800896 6
TGTAG 2982325 1.8028036 7.0994525 3
CACCC 1136770 1.7854344 8.626011 50-54
ACACG 1539290 1.7669821 6.904291 95-97
GCTTG 2346790 1.7647163 6.473137 65-69
CTATG 2649245 1.7614406 5.4633427 35-39
GGTAT 2906430 1.7569253 5.041761 30-34
CTCTA 2396025 1.7522242 6.485458 95-97
GCACC 1223745 1.7474699 8.045172 50-54
CCGCC 982190 1.7447028 5.896381 75-79
TGGTA 2880215 1.7410785 12.169024 2
CATGC 1746530 1.7017995 6.155084 2
GTAGC 1902255 1.6851892 6.2825084 5
CCACA 1327410 1.6759814 7.2210402 60-64
CTTTC 2692610 1.6714463 11.021797 7
GCCTT 2015005 1.6665915 5.101736 35-39
AGCCT 1705830 1.6621419 5.432652 4
TACTT 3251145 1.6223637 9.662756 5
ATGCC 1661590 1.6190348 7.1143184 9
CCCAA 1263445 1.5952195 34.391945 2
ATCTC 2170870 1.5875673 6.9966493 45-49
GAACA 1719120 1.5863926 6.177355 80-84
CGCAG 1220550 1.5846084 6.2188077 95-97
ACACA 1553255 1.5765208 6.45972 85-89
GCTGA 1773495 1.5711218 5.765517 80-84
CACGT 1610570 1.5693216 5.162612 95-97
AGAAA 2090530 1.5507926 6.7705755 9
GAGCC 1188060 1.5424275 5.1830745 95-97
TCCAA 1774520 1.5288277 5.5819488 25-29
CTGTA 2297890 1.5278304 5.5872965 2
AACAC 1503160 1.5256754 6.5441957 85-89
TGCAC 1500050 1.4616321 5.7254896 50-54
CCAAT 1686665 1.4531367 23.740902 3
ACGGT 1636070 1.4493784 7.015789 4
TTTAG 3184795 1.4449104 5.06433 6
GTATT 3177785 1.4417301 5.4924445 4
ACTTT 2879620 1.4369678 9.684534 6
GCAAC 1244825 1.4289596 6.8493867 1
GGTAC 1609590 1.42592 15.397191 3
GGTCA 1574975 1.395255 24.31113 9
ACATG 1771640 1.3877162 7.7964845 1
TTAGG 2263650 1.3683674 5.0035424 35-39
ATGGG 1688615 1.3600602 6.5570655 3
GGTCG 1331355 1.3339161 5.8480372 6
TGGTC 1772890 1.3331605 20.813595 8
GACGG 1126355 1.3295027 8.985518 3
GCTGT 1758240 1.3221442 5.285078 7
AAGCC 1145635 1.3150976 5.0525575 3
GTCAA 1669025 1.3073384 5.065402 10-14
GACTG 1464880 1.2977228 9.371724 1
TGTAT 2830845 1.2843268 5.1818085 3
TGGGT 1874405 1.2814795 5.0868545 8
ACGCA 1111695 1.276137 5.1350727 95-97
AACGC 1082670 1.2428186 5.6169963 95-97
ATTCG 1836785 1.2212489 6.285627 6
GGGTC 1206475 1.2087959 6.8902445 9
GTAGA 1677285 1.1944822 5.3981457 4
GTACT 1757755 1.1687032 11.575022 4
CAATT 1942380 1.1418928 16.4259 4
TGCCT 1379780 1.1412029 5.023258 30-34
CTGAC 1160300 1.1305835 7.9418864 1
TGACG 1249050 1.1065212 6.758046 2
CTTAT 2209860 1.1027489 5.093678 95-97
ATTGG 1807050 1.0923544 16.655571 6
ACTTG 1571435 1.044822 6.4172473 8
TGGCC 906065 0.9984962 8.112073 1
CGGTA 1070695 0.9485182 6.6038284 5
TAGAA 1498555 0.943606 5.4704313 8
AATTG 1725095 0.9220442 13.827855 5
ACGCG 612395 0.7950566 5.068737 15-19
TTAGA 1478685 0.7903408 5.0620418 7
CTTGT 1129595 0.63751346 5.3571143 9
TGACC 596155 0.5808869 6.140861 1
Produced by FastQC (version 0.10.1)