FastQCFastQC Report
Mon 21 Jul 2014
1S15B_GATCAG_L003_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 1S15B_GATCAG_L003_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 11415935
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 264599 2.317804016928968 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 131994 1.156225924551953 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 120567 1.0561289986321751 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 110966 0.9720272583892603 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 102854 0.9009686898182234 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 68680 0.601615198404686 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 66314 0.580889782571467 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 66064 0.5786998612027836 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 66039 0.5784808690659153 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 62749 0.5496615038540426 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 61987 0.5429866235222959 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 53139 0.4654809264418551 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 46852 0.4104087838622066 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 46771 0.4096992493387532 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 46537 0.4076494829376657 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 44650 0.3911199564468438 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 43111 0.3776388005012292 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 42532 0.3725669426113586 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 39908 0.3495815279256583 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 39194 0.34332711249669867 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 38584 0.33798370435711134 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 35477 0.3107673615871148 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 35291 0.30913806008881445 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 34530 0.30247193944254236 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 33248 0.29124202266393423 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 33227 0.2910580692689648 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 28962 0.2536980107192271 No Hit
GGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTA 28836 0.2525942903494107 No Hit
GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA 28183 0.24687421573440985 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 26275 0.23016073584861862 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 25878 0.22668314071514947 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 25559 0.22388880104870956 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 24899 0.21810740863538552 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 24480 0.21443710042147227 No Hit
GGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCAT 24077 0.21090694717515474 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 23857 0.20897981637071342 No Hit
CGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTC 21868 0.1915568019614688 No Hit
GTATGGGCTTGGATGTTCTTATTTGGACATCTTGTTTGGGCTACTGGGTT 21837 0.19128525171175204 No Hit
GCTCATGGTAAGGCTTTATATGGTTTTGATGTTCTTTTATCATCTGCAGA 21335 0.18688788960343589 No Hit
GATGAAATTAGCAGTATTATCCGTAAACAGATAGAAGACTATAGTCAAGA 21027 0.18418990647721803 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 20710 0.18141308618172755 No Hit
GGTCCTTACGAGTTAATCGTTCTTCACTTCTTACTTGGTGTAGCTTGCTA 20138 0.17640254609018008 No Hit
GCGACTGGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGT 18687 0.163692242466342 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 17927 0.15703488150554465 No Hit
TACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAAC 16988 0.14880953684477005 No Hit
ATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCATCTC 16939 0.14838031225650813 No Hit
GGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTATTT 16874 0.14781093270065046 No Hit
CAAGGTGAAACAGGTGAAATTAAAGGGCATTATCTAAATGCTACTGCAGG 16354 0.14325589625378912 No Hit
ATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGATTTCACTAAA 15868 0.1389986891130687 No Hit
CTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGT 15777 0.13820155773486797 No Hit
TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC 15775 0.13818403836391852 No Hit
CCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCCAGAA 15654 0.13712411642147576 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 15402 0.13491667568184296 No Hit
GTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCTTTCAA 15395 0.13485535788351982 No Hit
GTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGG 15176 0.13293698676455323 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 14637 0.12821551629367195 No Hit
ACCCAATTTGGGAAGCTGCTTCCGTTGATGAATGGCTTTACAACGGTGGT 14385 0.12600807555403915 No Hit
TACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGA 14381 0.12597303681214023 No Hit
GGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCTGGTACTTT 14225 0.12460652587808181 No Hit
TAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTA 14038 0.12296846469430668 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 13786 0.12076102395467388 No Hit
GGCCCTGGAGATTTCTTAGTTCATCACGCTATTGCTTTAGGTTTACATAC 13638 0.11946459050441334 No Hit
CATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGC 13517 0.11840466856197063 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 12980 0.1137007174620388 No Hit
ATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTG 12809 0.1122028112458594 No Hit
GTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAG 12488 0.10939095220847 No Hit
TGGCCCTGGAGATTTCTTAGTTCATCACGCTATTGCTTTAGGTTTACATA 12423 0.10882157265261233 No Hit
ACCTCCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCC 12377 0.10841862712077459 No Hit
GCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTC 12139 0.10633382197778807 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
ACTTC 4099820 3.713846 7.615138 60-64
CTGCT 3672275 3.5332265 15.801313 5
AACTT 4542700 3.3826463 6.452001 60-64
AAAAA 3728995 3.3178828 7.3355713 45-49
CAACT 2877805 3.1429844 9.422278 9
TTCAA 3961360 2.9497612 9.135045 9
GGAAG 2763145 2.9458067 7.455091 15-19
CTACT 3248995 2.9431217 16.098692 2
TCAAG 3061200 2.9189928 6.6020656 10-14
CTTCA 3217560 2.9146457 7.8456016 60-64
TCAAC 2652660 2.8970928 9.058107 55-59
AGCTG 2855425 2.8919394 8.918247 3
TCTCT 3769710 2.83234 9.45021 20-24
AGGAA 2818135 2.8286893 8.175369 40-44
CTGGT 3321705 2.7903445 14.10044 1
CTTCT 3633005 2.7296278 7.4632235 40-44
ACCCA 1698185 2.7202206 48.394127 1
ACAAC 1975545 2.601294 8.759355 85-89
AGCAC 1848700 2.5855093 6.2400303 35-39
ACTGC 2213035 2.56712 16.875542 4
GCTGC 2077115 2.559149 9.202488 3
GAAGC 2070880 2.5286868 8.57377 15-19
AAGCT 2638850 2.516263 6.6007357 15-19
CAACA 1865855 2.45686 8.647597 85-89
CAAGC 1749620 2.4469402 9.314257 75-79
TATGC 3059875 2.4200444 8.411939 30-34
CAAGG 1974935 2.4115312 8.079959 10-14
GGTTC 2867190 2.4085364 6.2531404 3
CGGTG 2220375 2.3884816 16.34492 9
CAGCA 1701870 2.3801591 7.2590094 35-39
GCAGC 1586940 2.357313 9.712443 1
CTCTG 2432770 2.3406544 9.924397 45-49
CTGAA 2454230 2.3402195 7.0271325 80-84
CTGCA 2008680 2.3300686 6.2213717 95-97
GGTGT 3168675 2.3239937 13.522145 1
ACATC 2096940 2.2901654 7.0436726 90-94
GGTGG 2430140 2.2823765 9.814322 7
CCAAG 1628625 2.2777224 9.096199 75-79
TGGTG 3060725 2.24482 8.330909 6
CATGG 2166095 2.1937943 13.659779 2
GCTAC 1881090 2.1820643 20.785673 1
TCTGC 2264805 2.1790493 5.615126 85-89
ATGGT 3136885 2.1661012 7.4844284 1
CCGTG 1755520 2.162922 6.4682355 60-64
TCCTT 2855760 2.1456513 6.9149213 40-44
CGTGA 2104755 2.1316702 11.937986 9
GTTCC 2203185 2.1197627 5.8434114 70-74
ATGCA 2201755 2.099473 5.87898 1
CAGCT 1806735 2.0958123 6.9591436 2
TTTCA 3384280 2.0901968 7.637059 8
GGTAT 3006615 2.0761464 6.4234233 30-34
CCCAA 1291420 2.0686483 48.896786 2
AAGGA 2051555 2.0592384 7.2134614 10-14
CGGAA 1659815 2.0267482 5.8237886 95-97
GCTCC 1421835 2.006429 6.909476 70-74
CTCCT 1805135 1.9892321 8.476469 70-74
TGCAG 1961825 1.9869124 5.329129 65-69
TTCCA 2170665 1.9663099 6.536179 75-79
GCTGG 1818060 1.955707 10.473393 4
CCTTA 2155955 1.9529848 9.120745 95-97
GCTTC 2025825 1.9491184 9.217359 15-19
CTTTA 3148595 1.9446329 5.0182242 35-39
CACAA 1476480 1.9441514 8.353575 85-89
CCAAT 1763720 1.9262406 33.59877 3
CATCC 1445510 1.9205186 8.357846 90-94
CTTGG 2283620 1.9183178 7.4332924 2
TGCTG 2277105 1.9128451 12.439461 6
TCTGG 2274015 1.9102492 5.584006 45-49
GATGG 2154520 1.9051512 5.0918927 1
CTTTC 2526410 1.8981969 8.985204 7
CCAGC 1111205 1.8905599 6.7619348 1
TGGCT 2243670 1.8847586 7.055352 9
ATCCG 1622350 1.8819256 5.705815 95-97
AGCTC 1608185 1.8654944 5.6430483 70-74
CTCTA 2050830 1.8577564 6.124732 25-29
CTGTT 2829605 1.8561957 9.92262 8
CCTGT 1920590 1.847868 10.51259 4
CTCTC 1669670 1.8399516 5.3402877 3
GCAGG 1400075 1.8158022 6.0564904 90-94
GGCTC 1466260 1.8065336 6.4574175 1
AATGA 2303500 1.805564 6.5608196 75-79
TACTG 2265935 1.7921199 11.604439 3
TAGCT 2238865 1.7707105 6.2651553 2
ACGGT 1730285 1.7524115 8.28123 4
TGGTA 2527710 1.7454498 10.815889 2
CACCC 894940 1.7439326 7.7505636 50-54
TTCGG 2075990 1.7439017 12.595214 7
GCCTT 1812025 1.7434138 5.9642496 35-39
ATGAT 2671745 1.7369928 5.3060966 15-19
TATTC 2811130 1.7362084 7.9568305 5
GAAAA 1826825 1.7264074 5.4356265 40-44
GAACA 1495370 1.7191415 7.530237 80-84
GATCG 1686830 1.7084007 5.040654 95-97
TCGGT 2024580 1.7007157 15.070483 8
GGAGA 1592170 1.6974226 5.1902523 7
GCACC 991625 1.6871113 6.982814 50-54
AGCTT 2122730 1.6788597 5.5016017 15-19
CCTAC 1263440 1.6786188 5.209376 80-84
ATGCC 1440280 1.6707246 8.670503 9
GTGGC 1548685 1.6659372 8.389474 8
TTGGT 2889075 1.6546925 18.057438 7
CTGTA 2086145 1.6499246 8.135304 5
GCATG 1627940 1.6487576 5.1310124 3
ACACA 1248360 1.643775 8.084714 85-89
GGTAC 1616170 1.6368371 13.147022 3
TTAGG 2369860 1.6364504 6.005417 35-39
GCAAC 1169745 1.6359532 6.681969 1
TGTAG 2368885 1.6357772 7.631962 6
CTACC 1227115 1.6303569 7.1908712 20-24
TACTT 2637370 1.6288905 8.026016 5
AGATC 1705640 1.626405 5.458045 95-97
TGAAC 1694675 1.6159494 6.674934 80-84
CCTCC 993685 1.6060638 7.3634114 1
ACCAG 1145570 1.6021432 5.616362 6
GACGG 1233420 1.599662 10.627854 3
GCTGA 1575480 1.595627 6.5746894 80-84
CATGA 1664275 1.5869616 5.9941335 65-69
GCTGT 1880270 1.5794904 12.613138 7
TCCAA 1445525 1.5787249 6.967257 75-79
CGCAG 1061835 1.5772982 6.8626065 90-94
GTCAA 1648325 1.5717527 6.1942115 10-14
CCACA 979710 1.5693387 6.659731 60-64
AACAC 1182400 1.5569223 8.08995 85-89
CCGCC 748155 1.5484759 7.914947 45-49
TTCAT 2499265 1.5435944 6.1373043 60-64
AACAT 1704815 1.5305287 5.6911116 90-94
CAACC 950840 1.5230936 5.5978003 75-79
GCACT 1309580 1.5191126 5.5496316 35-39
ATCTC 1673360 1.515823 6.731105 45-49
TCATG 1904115 1.5059577 5.0894713 60-64
ATTCC 1662320 1.5058225 5.2555504 80-84
GTGTA 2179595 1.5050673 12.388428 2
GGTCA 1463550 1.4822655 28.601362 9
GTGTT 2583320 1.4795738 5.044047 70-74
GCTTG 1758005 1.4767836 7.1868997 1
GGAAT 1770145 1.4737053 5.531046 40-44
GTAGC 1432745 1.4510665 7.8173428 1
CACAC 890450 1.4263585 5.688707 95-97
GAATC 1495635 1.4261557 5.936296 40-44
ACTTT 2303800 1.4228712 7.9544263 6
AACCA 1079650 1.4216264 6.211367 75-79
ACATG 1481040 1.4122387 12.547133 1
TGGTC 1678415 1.4099253 23.897108 8
ATGGC 1383445 1.401136 5.005462 45-49
AGGTA 1682280 1.4005547 7.33341 6
GGCTG 1297035 1.3952346 5.939718 9
TTCTC 1855015 1.3937503 5.9267592 20-24
GCAAG 1132655 1.3830495 5.2367635 1
GCGGC 875500 1.3813063 7.687191 7
GTACT 1744865 1.3800076 10.086765 4
CTGAC 1189180 1.3794487 9.218984 1
TGTTT 3074900 1.3752786 7.2839236 9
CAATT 1835910 1.36708 22.694836 4
GTATT 2525195 1.3616838 7.358133 4
CAGGT 1343865 1.36105 5.7295203 95-97
GCCTG 1096590 1.3510746 8.9718485 6
GTCTG 1599750 1.343844 5.557628 90-94
TGCCT 1394845 1.34203 5.8670907 30-34
GTAAC 1405800 1.3404939 5.1023874 8
GTATG 1941055 1.3403493 5.92052 7
TGACG 1313975 1.3307778 8.0720005 2
AGCCT 1144115 1.3271732 6.9507055 2
ATGGG 1489355 1.3169738 13.045898 3
AACGC 920465 1.2873212 5.2339625 90-94
TAGGT 1821110 1.257524 5.671318 5
GGTCG 1150890 1.2380248 12.232945 6
TAGGA 1480670 1.2327076 5.134731 40-44
ATCCT 1351355 1.224133 5.6768227 90-94
ACCGC 717895 1.2213979 5.3285394 45-49
TGTAT 2232630 1.2039213 7.255886 3
ATTGG 1717720 1.1861306 20.204557 6
AAGCC 844840 1.1815554 7.8204236 1
TGGGT 1593630 1.1688123 8.982509 4
CGGTA 1144750 1.1593888 7.717884 5
TGGCC 940195 1.1583852 13.4797325 1
TACCC 841145 1.1175535 5.335487 20-24
ATTCG 1405310 1.1114547 10.096729 6
GGGTC 1031670 1.1097785 12.19957 5
GCTCA 933900 1.083324 6.2746334 1
AATTG 1642320 1.0677284 18.419588 5
GCGAC 718155 1.0667802 5.788534 20-24
GTAGA 1269435 1.0568473 5.497236 4
TCGTG 1240390 1.0419694 9.473537 8
CTTAT 1671255 1.0321993 6.2398596 95-97
CCAGG 686120 1.0191939 5.2564697 2
CGCCT 720145 1.0162358 5.098506 45-49
TGCGG 929795 1.0001906 5.499348 6
TTGGC 1187745 0.99774593 6.085711 3
GTTCG 1168250 0.9813695 5.866138 4
TGGGG 1041930 0.97857594 6.3519163 8
TTATG 1795875 0.96840596 5.543529 95-97
CGGCT 784430 0.9664719 5.784348 8
AACGG 782600 0.9556083 6.0313697 60-64
AGTAG 1118055 0.9308184 6.017329 9
GGGGT 959145 0.90082467 6.1296496 8
GGCCA 603570 0.89657027 5.6664333 1
GCCCA 512270 0.87155575 5.933058 2
GGCCT 694225 0.85533315 8.700001 5
GGCCC 444190 0.80267936 9.352542 1
CCCTA 589805 0.78362083 7.3598795 2
GCCCT 536430 0.7569856 8.53103 1
GCCTA 627910 0.7283755 6.719503 3
CACGG 487860 0.7246895 5.6036835 4
GTCGT 856980 0.71989214 9.352103 7
CCTAT 683150 0.6188355 5.109392 4
GGTAA 716980 0.59691 7.418275 7
CCTAG 341585 0.39623857 6.181718 3
CTAGG 387700 0.39265776 5.562239 4