FastQCFastQC Report
Mon 21 Jul 2014
1S15A_GATCAG_L002_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 1S15A_GATCAG_L002_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 19826143
Filtered Sequences 0
Sequence length 101
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[WARN] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 497534 2.5094845729701434 No Hit
GACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCT 201683 1.0172578700758892 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 191127 0.9640150381241577 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 186020 0.9382561197102229 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 174633 0.8808218522382292 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 168273 0.848742995548857 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 129499 0.6531729343422974 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 117552 0.5929141134511136 No Hit
TGACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTC 107755 0.5434995601514626 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 104298 0.5260629866333558 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 77424 0.39051468558458396 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 77340 0.3900910025717054 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 74405 0.37528731634791496 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 73208 0.3692498334143963 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 70040 0.35327093121440717 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 69916 0.3526454943858722 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 67129 0.33858829728001055 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 59306 0.29913029478300446 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 59156 0.2983737179742928 No Hit
ACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGACTG 57526 0.29015224998629335 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 56209 0.2835095056058054 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 55370 0.27927771932241185 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 54016 0.2724483526624417 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 53485 0.2697700707596026 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 52117 0.26287009026415276 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 50840 0.2564290996993212 No Hit
GTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATATTGATGGTAT 49995 0.25216705034357917 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 49849 0.25143064891643324 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 49417 0.2492517077073438 No Hit
TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC 49174 0.24802605327723098 No Hit
GCTAAAAACTATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGA 48807 0.24617496201858324 No Hit
TACAACATCGTAGCTGCTCACGGTTACTTTGGTAGATTAATTTTCCAATA 48321 0.24372365315835764 No Hit
ACCCAATTTGGGAAGCTGCTTCCGTTGATGAATGGCTTTACAACGGTGGT 43923 0.22154082112693327 No Hit
GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA 43294 0.21836824237573593 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 42336 0.21353623849076445 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 40373 0.2036351699874252 No Hit
ATACACTTGGGTAACTTATTATTATTTTACAAACCAAGATTTACCATGAC 38129 0.19231678092909954 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 37705 0.19017819048314138 No Hit
TACCATGACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTC 37284 0.18805473157335748 No Hit
ATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTG 36449 0.1838431206715295 No Hit
CTGCTGCTATCATCCCTACTTCTGCAGCTATCGGTTTGCACTTCTACCCA 35830 0.18072098037424628 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 34524 0.17413371829306387 No Hit
CATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGC 33240 0.16765742081049248 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 32631 0.16458571896712335 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 32265 0.16273967155386704 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 32101 0.1619124809096757 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 30027 0.15145154556788984 No Hit
CCACGTAGTTGGTTAGCTACTTCTCACTTTGTATTAGGATTTTTCTTCTT 28734 0.14492985347679577 No Hit
ACACTTGGGTAACTTATTATTATTTTACAAACCAAGATTTACCATGACTG 27789 0.1401634195819126 No Hit
ACTCTATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCC 27754 0.13998688499321327 No Hit
TTCATGCGTTGGAGAGACCGTTTCTTATTCTGTGCAGAAGCTATTTACAA 27589 0.1391546505036305 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 27180 0.1370917177385435 No Hit
GCTGCTATCATCCCTACTTCTGCAGCTATCGGTTTGCACTTCTACCCAAT 26689 0.13461518965136082 No Hit
ACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGACGG 26638 0.1343579535363989 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 25280 0.1275084114948631 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 24603 0.12409372816487806 No Hit
GCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATG 24413 0.12313539754051002 No Hit
TATACACTTGGGTAACTTATTATTATTTTACAAACCAAGATTTACCATGA 24405 0.12309504677737873 No Hit
GCTCATGGTAAGGCTTTATATGGTTTTGATGTTCTTTTATCATCTGCAGA 24247 0.12229811920553584 No Hit
GCGACTGGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGT 23766 0.11987202957226728 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 23618 0.11912554045433849 No Hit
GCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCT 23536 0.11871194513224281 No Hit
CCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGATT 23280 0.11742072071204168 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 23026 0.11613958398262335 No Hit
CTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGC 22176 0.11185231539992424 No Hit
TACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAAC 22023 0.11108060705503839 No Hit
GCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGT 21913 0.11052578406198321 No Hit
AACAAGTTTATACACTTGGGTAACTTATTATTATTTTACAAACCAAGATT 21109 0.106470532367289 No Hit
ACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGAT 20933 0.1055828155784007 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 20479 0.10329290977070024 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 8142525 4.0652533 6.637352 70-74
CTGCT 6564750 3.6408288 15.338624 4
ACTTC 6662885 3.402896 7.998019 60-64
AGCTG 5671775 3.377287 7.736625 80-84
CAAGC 3856285 3.142927 11.128528 75-79
AACTT 7424240 3.0925581 6.8598065 60-64
CTACT 5767290 2.9454937 15.047243 2
CTTCT 6969505 2.9362748 6.557258 20-24
ACAAC 3678755 2.7610245 10.563555 85-89
GCTGC 3779585 2.759393 11.256265 3
CAACT 4445860 2.7525399 10.000177 9
GGAAG 4267255 2.728126 8.4662895 15-19
ACCCA 2961505 2.725225 53.88982 1
TGCTA 6011920 2.719411 11.420695 4
CTGGT 5535185 2.7188768 11.761 1
GCTAC 4035650 2.7132335 19.668503 1
AGCAC 3326880 2.7114544 7.1732 65-69
TCAAG 4859680 2.6647773 6.898488 10-14
CTTCA 5194600 2.653007 7.992768 60-64
CGGTG 4088935 2.6439605 10.226879 9
AGGAA 4439570 2.6137319 8.06415 10-14
ACATC 4186585 2.5920167 8.194458 90-94
TTCAA 6196955 2.581334 8.456296 9
TCTCT 6121645 2.5790687 9.283388 20-24
ACTGC 3816275 2.5657442 16.855232 2
CAGCA 3135290 2.5553062 8.794098 60-64
CTGAA 4655455 2.5527914 7.5649753 80-84
GGTGT 5824115 2.5337436 9.406289 1
AAGCT 4579950 2.5113885 7.1165605 75-79
TGGTG 5756695 2.5044131 8.293352 8
TCAAC 3965120 2.4549024 8.583198 55-59
CAACA 3263410 2.4492946 9.962323 85-89
GGTGG 4224570 2.4193714 5.61698 7
CCAAG 2957905 2.4107347 9.767469 75-79
TATGC 5324375 2.4084094 8.7083435 8
CTTTA 6983080 2.3995032 9.284432 5
GAAGC 3277990 2.366182 9.387731 15-19
GCAGC 2673365 2.3660245 7.961713 1
CCAGC 2337670 2.3359785 8.430705 60-64
TGAAA 5110795 2.2857106 6.021888 70-74
TCTGC 4095710 2.2714925 5.3467274 95-97
GCTTC 4057445 2.2502706 9.487105 15-19
CAGCT 3298265 2.2174776 7.131123 2
CTTGG 4464740 2.1930757 8.906484 6
GAAAG 3700720 2.1787446 6.795765 10-14
CAAGG 2999450 2.165121 8.949398 10-14
TCCTT 5114005 2.154547 6.02557 40-44
ATGCA 3921800 2.1504962 5.7937593 1
CCGCC 1747125 2.1405733 9.455389 75-79
CATGG 3589745 2.1375318 15.413415 2
GCTGG 3278565 2.1199644 7.3278785 4
TAGCT 4666950 2.111032 5.1315784 1
CGTGA 3506950 2.088231 12.684256 9
GATGG 3907695 2.0608435 5.314726 90-94
CTCCT 3281515 2.0548549 7.260828 70-74
CTCTG 3688095 2.0454283 9.229799 20-24
CCGTG 2799440 2.0438104 5.3971047 60-64
GCTCC 2456815 2.025194 5.6877413 65-69
GTTCC 3650845 2.0247695 6.3852787 70-74
CACAA 2671890 2.0053399 8.878608 85-89
CCTTA 3917935 2.0009837 10.048899 95-97
TTCCA 3889325 1.9863718 6.813137 75-79
ACCAG 2413850 1.967322 6.9955635 60-64
GAAAA 3582060 1.9420356 5.185558 70-74
CCCAA 2101485 1.9338206 53.63035 2
GCCTT 3486545 1.933648 6.3936644 35-39
AGCTC 2854350 1.9190263 5.036662 65-69
ATCGG 3204860 1.90835 6.5741267 85-89
TTCGG 3863465 1.8977301 7.783602 7
TCGGT 3860180 1.8961161 9.859562 8
AAGGA 3214530 1.8925072 7.8258667 10-14
GGTAT 4701360 1.8834777 7.4965262 6
CTATG 4153600 1.878825 6.374366 35-39
AAACC 2479380 1.8608551 6.5187182 70-74
TCGGA 3122670 1.8594096 6.914694 85-89
CATCC 2443640 1.8549644 8.965925 90-94
GCAAG 2566800 1.8528172 7.7108693 25-29
TTTCA 5383205 1.8497596 7.150348 8
AGAAA 3389585 1.837684 12.158859 9
GCACT 2712385 1.8235809 5.4213333 65-69
CCTGT 3243720 1.7989765 6.3758044 1
TTGGT 5432430 1.7953103 21.482134 7
TACTT 5177770 1.7791686 11.047937 3
CACTG 2635190 1.7716813 5.88453 65-69
CACCC 1569010 1.770256 7.3572335 50-54
CCACA 1908155 1.7559153 9.328666 1
CCAAT 2834115 1.7546694 36.374897 3
TATTC 5100725 1.7526947 5.279181 5
TGCTT 4690790 1.7503146 5.9659886 4
GGTCG 2702390 1.7474018 13.522819 6
TCTGG 3547740 1.7426462 6.047851 45-49
GCACC 1728490 1.7272394 6.6252637 50-54
ACCGC 1728455 1.7272044 7.472104 75-79
AGCCT 2566500 1.7255 5.793679 4
CTTTC 4093970 1.7248026 8.577536 7
ACACA 2296100 1.7232972 8.630121 85-89
ACGGT 2891585 1.7218088 11.262066 4
AAAAC 2808610 1.7192556 5.6656847 70-74
AAGCC 2104755 1.7154052 7.17084 2
GTGTA 4274895 1.7126257 8.5629835 2
GAACA 2564900 1.7049639 7.953552 80-84
ATGGG 3229420 1.7031342 12.790117 3
CCTTT 4009485 1.6892091 5.1241336 35-39
GCTTG 3437225 1.6883614 6.169032 5
TGGTA 4203480 1.6840152 9.209554 2
TTTAG 5529585 1.6828376 7.788222 6
GTAGC 2811730 1.674259 5.384145 1
TCCAA 2686200 1.6630917 7.358155 75-79
CATGC 2463895 1.6565169 8.300625 2
CTCTA 3230200 1.6497409 5.4803467 95-97
AACAC 2193400 1.6462176 8.722876 85-89
AGCTT 3631110 1.6424837 5.810993 15-19
AAAGC 2459955 1.6352038 6.950627 20-24
ACTTT 4745540 1.6306472 11.395539 4
GGATG 3087680 1.6283829 5.699975 85-89
GACTG 2733050 1.6274084 13.828579 1
GCTGA 2724855 1.6225286 7.2004004 80-84
GGGTC 2481605 1.6046393 13.44756 5
TGAAC 2891005 1.5852658 7.1948385 80-84
CTGCG 2170800 1.5848538 7.312858 45-49
ACATG 2884095 1.5814767 16.2921 1
CATGA 2863620 1.5702492 6.356496 65-69
TTAGG 3911290 1.5669566 6.8725166 35-39
GCGAC 1768280 1.5649916 6.7715654 50-54
ATGCC 2307765 1.5515481 12.034057 9
CTGTA 3356080 1.5180776 7.266495 8
GATCG 2549165 1.5179132 5.081486 50-54
AACAT 2994460 1.512084 6.1748943 90-94
GGTAC 2524895 1.5034614 11.907741 3
CTACC 1977635 1.5012206 5.088797 25-29
CATCG 2228180 1.4980419 5.20535 85-89
ACTGG 2504380 1.4912456 5.9881554 50-54
GACGG 1900525 1.4897379 14.657727 3
TGGGT 3418470 1.4871835 9.65685 4
CTGGG 2280610 1.4746732 5.1260986 55-59
GCAAC 1803515 1.4698904 9.4772215 1
CGCCT 1737765 1.4324687 6.648402 75-79
TTCAT 4161100 1.4298238 5.1613283 60-64
AACCG 1753970 1.4295105 7.7576447 70-74
GCGCA 1611545 1.4262755 7.1933403 25-29
GCGGC 1475930 1.4184778 7.652402 7
GACGC 1600570 1.4165622 7.198684 15-19
GTGTT 4251225 1.4049457 5.1712427 70-74
AGCGC 1561615 1.3820857 6.7844872 25-29
GGCTG 2135510 1.3808496 5.845947 9
TGGGG 2399350 1.3740853 5.3903575 9
ATTCC 2685665 1.3716338 5.122458 80-84
GGAAT 2819030 1.3690782 5.926419 40-44
GCGAA 1892560 1.3661243 5.690807 20-24
TCATG 3016960 1.3646812 5.3899508 60-64
GTCAA 2487715 1.3641241 6.529341 10-14
TGGTC 2773880 1.362527 26.28931 8
CGCAA 1670320 1.3613347 6.569916 25-29
ATGCT 3000615 1.3572879 6.1181355 3
GTATT 4450385 1.3544011 5.263924 4
ATCTC 2630350 1.3433831 6.279782 45-49
GGTCA 2252360 1.3411791 31.519762 9
AAGAC 2001065 1.3301663 5.4551115 15-19
GTATG 3245290 1.3001412 7.6612945 7
CAATT 3113210 1.2968038 24.825237 4
AAGCG 1793840 1.2948643 5.593037 25-29
GAATC 2356980 1.2924362 6.369724 40-44
TTCTC 3054855 1.2870202 5.5916405 20-24
GTCGC 1749525 1.2772902 5.8136253 40-44
CGCGA 1437890 1.2725846 6.548297 20-24
TGCCT 2290325 1.2702209 6.326904 30-34
CTGAC 1886955 1.2686307 12.69909 1
GGGGT 2210180 1.2657493 5.555441 35-39
AGACG 1749155 1.2626089 6.0412655 15-19
GCCTG 1718805 1.2548622 5.409153 6
CACAT 2015265 1.2476995 6.3123193 2
GTACT 2745175 1.241743 9.107119 4
CGACT 1825770 1.2274951 5.4109716 50-54
ATCCT 2388155 1.2196882 5.9757137 90-94
TGACG 2042860 1.2164314 11.061438 2
TGTAT 3970295 1.2082937 5.365032 9
CGAAA 1817540 1.2081718 5.5197935 20-24
TACAT 2866080 1.1938621 5.547626 4
GCCTA 1766565 1.1876906 5.399826 4
TCTGA 2569520 1.1622877 5.280771 20-24
ACGCG 1310755 1.1600655 8.303097 15-19
CTTAT 3350100 1.1511506 6.5174804 95-97
TAGGA 2363235 1.1477188 5.587392 40-44
TAGAA 2565305 1.1472863 9.82592 8
ATTCG 2514720 1.1374997 6.454632 6
ATTGG 2788655 1.1172022 21.93238 6
TGGCC 1527065 1.114877 8.188821 1
CGCTT 1994615 1.1062193 5.488651 40-44
GCTCA 1617740 1.0876331 6.508594 1
CGGTA 1822060 1.0849547 10.761061 5
TGCGG 1669755 1.0796862 5.4781137 6
TCGTG 2165915 1.0638952 10.440546 8
TTATG 3364155 1.0238248 5.909813 95-97
CGGCT 1380450 1.0078366 5.687094 8
AATTG 2707460 0.9988568 19.804258 5
TTAGA 2557280 0.9434512 8.631352 7
GGCCT 1103760 0.80583125 5.29869 5
CACGG 905025 0.8009798 6.361953 4
CTACA 1283215 0.7944696 8.463217 3
GTCGT 1592740 0.78235227 10.213536 7
TGACT 1538525 0.6959311 5.8828363 1
GGCCC 635060 0.68912214 5.129931 1