FastQCFastQC Report
Mon 21 Jul 2014
1L22C_ATCACG_L004_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 1L22C_ATCACG_L004_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 14486208
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 159578 1.1015857289913273 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 132462 0.9144007872867765 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 113219 0.7815640918589599 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 107510 0.7421541924567147 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 85438 0.5897885768311487 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 84486 0.5832168087052181 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 84135 0.5807938143646701 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 70531 0.48688380009454507 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 66355 0.4580563802480263 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 57356 0.3959352233517564 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 56970 0.3932706198889316 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 49579 0.3422496763818385 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 47838 0.33023134832800966 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 46032 0.3177643176185238 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 43128 0.2977176635873239 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 35925 0.2479945062227465 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 35812 0.2472144539136812 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 34688 0.2394553495297044 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 34384 0.2373568017247854 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 33908 0.23407091766182012 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 33459 0.23097141777889701 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 31365 0.21651628914896157 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 31198 0.215363468479812 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 30216 0.20858460682050126 No Hit
GGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCAT 28779 0.19866482657159143 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 28670 0.19791238673364348 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 27136 0.18732300406013774 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 26643 0.1839197669949237 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 24925 0.17206021065001967 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 24896 0.17186002023441882 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 23407 0.16158127786098336 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 23382 0.1614086999164999 No Hit
ATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAA 23255 0.16053200395852388 No Hit
CCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCCAGAA 23165 0.15991072335838338 No Hit
GCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGA 21765 0.15024635846730905 No Hit
GCTCATGGTAAGGCTTTATATGGTTTTGATGTTCTTTTATCATCTGCAGA 20922 0.14442703017932643 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 20858 0.14398523064144875 No Hit
GGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTA 20531 0.14172791112760497 No Hit
GTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGG 19357 0.1336236508546612 No Hit
TACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGA 19180 0.13240179900771823 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 18631 0.12861198734686125 No Hit
ATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCATCTC 18617 0.12851534369795048 No Hit
GGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTATTT 18385 0.1269138203731439 No Hit
CTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGT 18108 0.12500165674826705 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 17960 0.1239799953169249 No Hit
GCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATG 17486 0.1207079174895183 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 17447 0.12043869589612409 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 16812 0.11605521610624395 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 16514 0.11399808700800099 No Hit
TGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCT 16226 0.11200998908755143 No Hit
ACCAATAGGGGTGCTTCGTGCACCCCCAGAAGCATGTGTCGAGAGCTCCC 16132 0.11136109601629356 No Hit
CTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCC 15770 0.10886216738017292 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 15686 0.10828230548670845 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 15670 0.10817185560223905 No Hit
GGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCTGGTACTTT 15027 0.1037331508701242 No Hit
CCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCATCTCTG 14924 0.10302212973885229 No Hit
GTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAG 14800 0.1021661431342143 No Hit
GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA 14519 0.10022636703822006 No Hit
CATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGC 14492 0.10003998285817793 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGCT 4556965 3.5742304 8.835095 5
ACTTC 4792280 3.4579625 6.0934043 55-59
AAAAA 5203485 3.331777 5.541897 45-49
GGAAG 3868815 3.181602 6.6000476 85-89
AACTT 5353375 3.0886633 5.764344 55-59
AGCAC 2789905 3.0371776 7.466097 90-94
GAAGA 3958745 2.994999 6.422089 90-94
AGCTG 3611300 2.9003534 9.399567 3
CTACT 4019505 2.9003518 9.418727 2
CTTCA 3866585 2.7900097 6.192318 60-64
TCTCT 4476565 2.7418017 8.20992 20-24
CGGAA 2887955 2.7325287 7.266665 95-97
CTGGT 3971480 2.707402 13.6371975 1
TTCAA 4641305 2.6778305 7.650356 9
CTTCT 4102025 2.512404 5.577603 40-44
CAACT 2935610 2.4955335 9.351553 9
GCTGC 2473830 2.4848053 6.9833894 3
CGGTG 2823620 2.4650307 14.38849 9
GGTGG 3247990 2.464475 12.019682 7
TGGTG 4149220 2.4584446 10.51005 6
CCGTG 2437900 2.4487162 6.8890867 60-64
GGTGT 4131990 2.4482358 12.096479 1
AGAGC 2564025 2.4260323 7.2721715 95-97
CTGAA 3276345 2.4207406 5.1910076 80-84
ACATC 2834480 2.409564 5.69148 40-44
TATGC 3839765 2.4081101 6.767858 30-34
TCTGC 3067080 2.4056475 6.2260017 85-89
ATGCA 3200875 2.3649793 7.0735455 1
ACAAC 2354170 2.357707 5.279762 40-44
CATGG 2926250 2.3501675 9.215752 2
ATCGG 2890195 2.3212104 6.2981486 95-97
TCGGA 2883455 2.315797 6.0723658 95-97
AAGAG 3056280 2.3122365 5.7950068 90-94
CAACA 2301750 2.3052084 5.1439495 85-89
GATCG 2864010 2.3001802 6.9222636 95-97
GAGCA 2424900 2.294395 6.956356 90-94
ACCCA 1815800 2.2743392 24.784891 1
CGTGA 2826160 2.2697816 7.4076667 9
TCAAC 2653995 2.2561355 8.383255 8
GCACA 2056845 2.2391455 7.727649 95-97
GCAGC 1891310 2.2380624 5.7087855 1
CAAGC 2027820 2.2075481 5.5435367 75-79
GAAGC 2325975 2.2007937 5.1439295 15-19
GTTCC 2794690 2.1919994 5.0965037 8
TCCTT 3578465 2.1917343 6.8022995 40-44
ATGGT 4019235 2.19083 5.685398 5
CAGCA 1991480 2.167987 5.594482 2
GCTGG 2475030 2.1607103 10.897589 4
CACAC 1706395 2.1373062 9.011746 95-97
AGATC 2876425 2.125258 6.410283 95-97
ACTGC 2291140 2.1171198 8.896942 4
GGCTC 2069425 2.0786064 6.577687 10-14
CCAAG 1904745 2.0735648 5.963328 25-29
CTCTC 2297585 2.0734084 5.9390635 15-19
ATCCG 2232710 2.0631278 5.325039 65-69
CCTTA 2814790 2.0310664 7.2176194 45-49
TGGCT 2907375 1.9819899 9.515738 9
TATTC 4039290 1.9781581 7.400318 2
CTCTG 2515810 1.9732617 6.2318387 45-49
GTGGC 2215535 1.9341704 11.367201 8
GCATG 2405985 1.9323254 6.484985 3
CTCTA 2668395 1.9254323 5.539419 25-29
CTTGG 2802000 1.9101549 5.724378 2
GCAGG 1821775 1.8736901 6.525267 90-94
TGCTG 2746205 1.8721185 6.923333 6
GCTAC 2007325 1.8548615 11.612835 1
TAGCT 2956425 1.8541231 6.868872 2
TTCGG 2713530 1.849844 11.076021 7
CATCC 1734970 1.8445575 5.183842 40-44
CACCC 1170410 1.8334134 7.3038387 50-54
GCTCT 2325635 1.8240988 6.293522 10-14
TACTG 2897270 1.8170238 6.284682 3
TTTCA 3704135 1.814023 6.6438203 8
GTCTG 2641785 1.8009342 7.2655435 90-94
CTCCT 1992310 1.7979193 5.7391 70-74
AATGA 3030025 1.7900685 5.7655377 75-79
TCGGT 2599385 1.7720296 12.084252 8
GCACC 1287125 1.7524137 6.5765533 50-54
CACAA 1742680 1.7452984 5.2699485 35-39
CAGTG 2168590 1.7416657 5.690503 30-34
CCAGC 1277030 1.7386694 6.5375867 1
CGCAG 1448880 1.7145176 7.5780344 90-94
GCTTC 2175085 1.7060158 6.163804 15-19
CCTGT 2152185 1.6880544 7.9580855 1
ACTAC 1976115 1.6798762 5.301922 70-74
CTGAG 2079060 1.6697613 5.540804 80-84
CTGTT 3130020 1.6662179 5.5661464 8
GTGTA 3037275 1.6555768 10.856968 2
AATCC 1936645 1.6463231 5.230339 60-64
TTGGT 3552665 1.6437377 10.981362 7
CTTTC 2660980 1.6297944 7.904573 7
AGTGC 2015525 1.6187341 5.031523 25-29
ACACG 1485735 1.6174175 7.6861157 95-97
CCACA 1284660 1.6090717 6.136409 60-64
TGTAG 2940720 1.602946 6.7867947 3
CCCAA 1275565 1.5976799 24.646454 2
GTAGC 1958245 1.5727308 8.122363 1
TGGTA 2811315 1.5324092 9.552578 2
CCAAT 1800800 1.5308427 17.186752 3
GCTTG 2238000 1.5256695 5.3029094 1
TACTT 3032990 1.4853436 6.9963794 5
ACACA 1463845 1.4660444 5.162912 35-39
ATGCC 1568190 1.4490805 6.0306945 9
GCTGT 2092285 1.4263339 7.17862 7
GTATT 3349960 1.4259002 6.8600383 4
AACGC 1306460 1.4222531 6.300208 95-97
CACGT 1529410 1.4132458 5.6620092 95-97
CAGGT 1754295 1.4089317 5.643132 95-97
CCGCC 820430 1.3969879 5.9014964 45-49
ACATG 1884915 1.3926771 8.125766 1
ACGGT 1692645 1.3594186 5.8622665 4
GGTCA 1674845 1.3451229 15.212569 9
AGCCT 1442610 1.3330386 5.6390014 2
GGTAC 1658785 1.3322246 11.073777 3
ACTTT 2690820 1.317773 6.829912 6
GCGGC 1007825 1.2963532 5.3840666 7
ACGTC 1392090 1.2863557 5.420161 95-97
GCCTG 1278090 1.2837604 5.8826466 3
GACGG 1237165 1.2724205 7.4823213 3
TGTAT 2972425 1.2652036 6.639193 3
ATTCG 2017100 1.2650249 9.107406 6
TGGTC 1852960 1.2631834 13.089511 8
CAATT 2085555 1.203274 11.690947 4
ATGGG 1719495 1.2002782 7.895442 3
CTAAC 1405990 1.1952184 5.382006 95-97
GTACT 1811500 1.1360828 8.557883 4
AAGCC 1037055 1.1289704 5.722638 1
CTGAC 1210060 1.1181517 6.6069436 1
GGTCG 1268885 1.1077414 7.205795 6
TGGGT 1867525 1.1065229 5.7731867 4
TGACG 1366695 1.0976375 5.7785373 2
ATTGG 1981665 1.0801785 10.808071 6
TGGCC 1048290 1.0529408 8.679076 1
GGGTC 1145920 1.0003924 7.1850524 5
TCGTG 1439815 0.9815379 5.644122 8
AATTG 1854045 0.92972976 9.263657 5
GGGGT 1160085 0.8802368 5.4369674 8
CGGTA 1094225 0.8788079 5.3345733 5
GGCCT 746805 0.7501184 5.5431705 2
GCCTA 769925 0.71144646 5.3571634 3
GCCCT 596610 0.68947655 5.4609127 1
GTCGT 979080 0.6674497 5.400493 7
CCCCG 382635 0.651532 5.66123 1
Produced by FastQC (version 0.10.1)