FastQCFastQC Report
Mon 21 Jul 2014
1L22B_ATCACG_L003_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 1L22B_ATCACG_L003_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 16829092
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 182756 1.0859528250246657 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 148635 0.8832027301294686 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 131530 0.7815632596220877 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 117731 0.6995683427246104 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 111587 0.6630601342009421 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 107236 0.6372060952545746 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 105047 0.6241988575497716 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 103549 0.615297604885635 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 90010 0.5348476317082348 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 86145 0.5118814491001653 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 75005 0.4456865527860921 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 65500 0.38920697563481144 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 63576 0.3777743921062408 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 62318 0.37029924133756 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 58624 0.3483491563300028 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 52311 0.31083673438828424 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 52157 0.3099216523387001 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 48824 0.29011666226555777 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 45880 0.27262314568129997 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 41004 0.24364950883862302 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 40497 0.24063686858447264 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 40486 0.2405715055809309 No Hit
GGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCAT 39283 0.23342316982995875 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 39086 0.2322525778574388 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 38291 0.22752861532874144 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 37893 0.22516366301877727 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 36812 0.21874026239799507 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 32784 0.19480551891926196 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 30968 0.18401468124364642 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 30446 0.18091290962103007 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 29663 0.1762602521871055 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 27282 0.1621121329659378 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 27207 0.16166647612360785 No Hit
TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC 27064 0.16081675707756543 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 26829 0.15942036563826498 No Hit
TGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCT 26091 0.1550351023097384 No Hit
CATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGC 25218 0.14984765666501793 No Hit
CTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCC 24991 0.14849880195556597 No Hit
GGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTATTT 24651 0.1464784909370036 No Hit
GGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCTGGTACTTT 24145 0.1434717927740843 No Hit
ATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCATCTC 24100 0.14320439866868637 No Hit
GCTCATGGTAAGGCTTTATATGGTTTTGATGTTCTTTTATCATCTGCAGA 23938 0.14224177988925368 No Hit
GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA 23273 0.13829028922059491 No Hit
GTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCTTTCAA 23166 0.13765448545887085 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 23063 0.13704245006207108 No Hit
GTATGGGCTTGGATGTTCTTATTTGGACATCTTGTTTGGGCTACTGGGTT 22623 0.13442792992040212 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 22604 0.13431503018701188 No Hit
TACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAAC 22222 0.13204515133674474 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 21427 0.1273211888080474 No Hit
GTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGG 20756 0.12333404559200223 No Hit
ACCAATAGGGGTGCTTCGTGCACCCCCAGAAGCATGTGTCGAGAGCTCCC 20203 0.12004806914122283 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 20096 0.1194122653794988 No Hit
TACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGA 19529 0.11604309965148446 No Hit
GCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGA 19352 0.11499134950358582 No Hit
GGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTA 19183 0.11398713608553569 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 19052 0.11320872213426607 No Hit
ATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGATTTCACTAAA 19038 0.11312553285703114 No Hit
GTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAG 19026 0.11305422776225836 No Hit
CCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCATCTCTG 17764 0.1055553086286533 No Hit
CTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGT 17732 0.10536516170925918 No Hit
ATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAA 17666 0.10497298368800884 No Hit
GCGACTGGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGT 17563 0.10436094829120905 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 6306575 3.6946278 6.2477536 95-97
CTGCT 5386595 3.5875218 9.230049 5
ACTTC 5587175 3.5244274 7.3344755 55-59
AACTT 6300480 3.2304282 6.979578 55-59
GGAAG 4609785 3.178408 5.9388638 85-89
GAAGA 4714840 3.079016 5.813159 85-89
AGCAC 3284075 3.074636 6.7626777 90-94
AGCTG 4299390 2.913476 11.278632 3
CTACT 4616585 2.9121728 9.609124 2
TTCAA 5620550 2.8818097 7.395343 55-59
TCAAG 4464695 2.8655794 5.4497423 60-64
TGCTA 5264775 2.850035 5.599566 95-97
CTTCA 4517025 2.8493695 6.334596 60-64
CAACT 3681645 2.7535212 8.112125 9
CTGGT 4766665 2.724388 12.511798 1
TCTCT 5031210 2.6768157 8.672379 20-24
CGGAA 3275335 2.6315434 6.234212 95-97
TCAAC 3443850 2.575673 7.7069907 8
GCTGC 3001270 2.5021853 7.351431 3
TATGC 4606620 2.4937491 6.453995 35-39
CTTCT 4682870 2.4914842 5.847378 40-44
ACATC 3316015 2.4800644 5.490341 40-44
TGGTG 4989045 2.4470673 10.816184 6
CTGAA 3775835 2.4234476 5.016208 80-84
AGAGC 3013225 2.420953 5.9356565 90-94
GGTGG 3925560 2.410262 10.182068 7
GAAAC 3135450 2.3860016 5.8176823 75-79
GGTGT 4854815 2.381229 12.313876 1
AAGAG 3622590 2.3657246 5.1641583 90-94
CGGTG 3297680 2.3593752 13.63442 9
ACAAC 2657635 2.3566337 5.2633033 40-44
GAGCA 2889115 2.321238 6.2845426 90-94
ATGCA 3604520 2.313492 6.5009704 1
CATGG 3412615 2.3125541 11.098877 2
CCGTG 2769715 2.309136 6.554756 70-74
GCACA 2460005 2.3031206 6.7134147 95-97
TCTGC 3438195 2.2898698 6.1640615 90-94
GATCG 3369305 2.283205 5.9071517 95-97
ACCCA 2076555 2.2654266 24.165554 1
ACTGC 2866920 2.2638419 9.371129 4
TCGGA 3310755 2.2435286 5.2131014 95-97
CACAC 2047950 2.2342198 8.073033 95-97
ATCGG 3294115 2.2322526 5.448432 95-97
CGTGA 3271130 2.216677 9.522321 9
CAGCA 2362705 2.212026 6.1829057 35-39
GCAGC 2236975 2.2111883 6.106888 1
TCCTT 4099000 2.1808407 6.889713 40-44
GAGAA 3333455 2.1769056 5.1502404 80-84
GCTGG 3010855 2.154162 12.740402 4
ATGGT 4598105 2.1361089 6.508535 1
CAAGC 2255930 2.1120603 6.059318 2
GTTCC 3156245 2.102088 5.8686557 45-49
AGATC 3272980 2.1007 5.8001757 95-97
CAAGG 2593495 2.0837243 5.4457884 4
CAAGT 3213245 2.06236 5.3149633 60-64
CTCTC 2648710 2.0556087 6.3364043 20-24
TATTC 4738135 2.049008 7.06989 5
TGGCT 3549250 2.0285742 7.8260374 9
CCAAG 2166565 2.0283945 5.230829 25-29
GGCTC 2403990 2.0042276 6.5765514 20-24
CAGCT 2525775 1.994459 5.7020364 65-69
ATCCG 2504970 1.9780306 5.5786304 65-69
CCTTA 3127965 1.9731412 7.223475 45-49
CTTGG 3419790 1.9545816 6.4062314 2
TAGCT 3604840 1.9514451 8.457605 2
CTCTG 2920915 1.9453562 6.0258837 45-49
TTTCA 4480395 1.9375479 5.91531 8
CTCTA 3066730 1.9345138 6.8707037 25-29
GCTAC 2405010 1.8990977 11.533878 1
TGCTG 3282285 1.8759904 7.2967787 6
GTGGC 2618470 1.8734244 9.094703 8
CTCCT 2400795 1.8632075 7.224118 70-74
GCTCC 1915880 1.8612665 5.8349824 70-74
TACTG 3424650 1.8539011 6.727337 3
GCATG 2724170 1.8460301 5.7351093 3
TTCCT 3451805 1.8365058 5.793569 45-49
TCCGT 2738220 1.8236797 5.0416317 70-74
AAACT 2979145 1.8110411 5.0662317 75-79
GTCTG 3162530 1.8075445 7.9151034 90-94
CTGTT 3932095 1.7953293 5.953805 8
TTCGG 3139330 1.7942845 10.712771 7
AATGA 3437215 1.7931536 5.418891 75-79
GCTCT 2634190 1.7543945 6.8443246 20-24
GCAGG 2065290 1.7519423 5.524921 90-94
CACAA 1974255 1.7506528 5.295267 4
CCTGT 2626100 1.7490067 8.355508 1
CACCC 1298850 1.7433131 6.395815 50-54
ACACG 1840665 1.7232784 6.9300156 95-97
CTATG 3179560 1.7212241 5.825775 35-39
TCGGT 3001110 1.7152848 11.995859 8
CTTTC 3219145 1.7127209 6.9658747 7
CTATT 3959750 1.7123952 5.591459 25-29
CAGTG 2514650 1.7040492 6.8055944 30-34
GCACC 1458695 1.6801769 5.7470083 50-54
GTGTA 3591310 1.6683893 11.362358 2
ACTAC 2214925 1.6565537 5.775664 75-79
TGTAG 3554115 1.6511099 7.5687475 3
CCAGC 1427710 1.6444873 6.406224 1
CTGAG 2411945 1.6344513 6.6192555 80-84
GCTTC 2443540 1.62742 5.893305 15-19
CCAAT 2173110 1.6252801 16.954056 3
AATCC 2169270 1.6224082 5.8261027 65-69
CCCAA 1473650 1.6076849 24.48771 2
AAGTT 3651140 1.6065308 5.098275 60-64
TTGGT 4083510 1.6000292 10.711635 7
GTAGC 2350385 1.5927353 9.971116 1
CCACA 1457925 1.5905296 6.3092413 1
AGTGC 2340760 1.5862129 5.445514 30-34
GCTTG 2755055 1.5746521 5.9856534 1
ATGCC 1991945 1.572924 7.7434206 9
CGCAG 1566990 1.5489266 6.5372677 90-94
TGGTA 3291755 1.5292273 8.858517 2
CATGC 1923865 1.5191655 5.8523602 2
GCTGT 2641025 1.5094782 7.431032 7
CACGT 1907760 1.5064481 5.4095287 95-97
CTGTA 2779290 1.5045416 6.259526 5
TACTT 3388980 1.4655654 6.295834 5
ACGGT 2152505 1.458642 7.1698723 4
CCTCC 1285905 1.4557106 5.0480585 1
GTATT 3838755 1.4246255 6.4749928 4
CTACC 1530185 1.4079922 5.1049676 20-24
ACATG 2187610 1.4040756 10.74091 1
ACGTC 1763390 1.3924475 5.0915165 95-97
GGCTG 1921385 1.3746841 5.2180386 9
AACTA 2248425 1.366832 5.885842 75-79
AACCA 1540640 1.3661486 5.1055136 75-79
GGTAC 1970735 1.3354659 9.817909 3
CAGGT 1964705 1.3313795 5.219565 95-97
AACGC 1411700 1.3216704 6.351657 95-97
GCGGC 1264925 1.3201199 6.482788 7
ACTTT 3011600 1.3023673 6.141904 6
TGTAT 3460285 1.2841691 6.6235886 9
GCCTG 1538930 1.2830195 7.598864 3
GACGG 1511640 1.2822926 9.303503 3
GGTCA 1876165 1.2713807 13.767208 9
ATTCG 2345610 1.2697734 8.664735 6
CCGCC 894125 1.2670641 5.63882 45-49
GTATG 2702465 1.2554649 5.157346 7
AGCCT 1544050 1.2192473 5.3189344 2
CAATT 2365525 1.2128692 11.654949 4
TGGTC 2118395 1.2107689 11.750281 8
ATGGG 2012635 1.1704252 9.791243 3
GTACT 2130145 1.1531334 7.697611 4
GTGAG 1978975 1.1508508 5.210867 8
CTGAC 1451030 1.1457949 8.433811 1
CTAAC 1513675 1.1320853 6.5833097 95-97
TGGCC 1334620 1.1126846 9.837026 1
TGACG 1635285 1.1081487 7.317096 2
GGTCG 1521900 1.0888666 9.320063 6
AAGCC 1141580 1.0687768 5.928705 1
TGGGT 2148530 1.0538284 7.0553875 4
ATTGG 2264780 1.0521327 10.1228285 6
TCGTG 1774155 1.0140185 7.444922 8
GGGTC 1328620 0.9505815 9.226036 5
AATTG 2110480 0.9286281 8.98306 5
CGGTA 1333550 0.90367836 6.763696 5
GGCCT 984910 0.8211282 7.081762 2
GTCGT 1248425 0.71353745 7.2223206 7
Produced by FastQC (version 0.10.1)