FastQCFastQC Report
Mon 21 Jul 2014
1L22A_ATCACG_L002_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 1L22A_ATCACG_L002_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 14807977
Filtered Sequences 0
Sequence length 101
%GC 45

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 290960 1.9648868984602015 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 251028 1.6952214336907734 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 158516 1.0704770813731006 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 128808 0.8698554839732666 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 111665 0.7540868006480561 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 89472 0.6042148768869644 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 86106 0.5814838853409889 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 77436 0.522934361661961 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 75485 0.5097590305549502 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 73967 0.4995077990734318 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 61382 0.4145198226604485 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 54952 0.3710972808777323 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 52421 0.3540051419582837 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 49200 0.33225335236541764 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 47511 0.3208473378909219 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 44231 0.29869711439989405 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 41166 0.2779988110462354 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 38853 0.26237885161491 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 38257 0.2583539939317842 No Hit
TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC 37317 0.25200606402886766 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 36742 0.2481230218010198 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 36541 0.24676564530050257 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 34619 0.23378615458411367 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 34537 0.23323239899683798 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 33964 0.2293628630028261 No Hit
GCTCATGGTAAGGCTTTATATGGTTTTGATGTTCTTTTATCATCTGCAGA 33649 0.22723563117365728 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 32730 0.22102951672601867 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 32281 0.21799736722983837 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 31501 0.21272993603380125 No Hit
ACCCAATTTGGGAAGCTGCTTCCGTTGATGAATGGCTTTACAACGGTGGT 31380 0.2119128088867237 No Hit
GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA 31153 0.21037985134633852 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 30661 0.20705731782268436 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 29216 0.19729906387617968 No Hit
GTATGGGCTTGGATGTTCTTATTTGGACATCTTGTTTGGGCTACTGGGTT 27591 0.18632524888443572 No Hit
CCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCCAGAA 26301 0.17761372806022052 No Hit
GACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCT 26110 0.17632388272888322 No Hit
CTGCTGCTATCATCCCTACTTCTGCAGCTATCGGTTTGCACTTCTACCCA 25219 0.1703068555549485 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 25100 0.16950323464170697 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 24704 0.16682900034218043 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 24357 0.16448566877163573 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 23298 0.15733411795547764 No Hit
CCACGTAGTTGGTTAGCTACTTCTCACTTTGTATTAGGATTTTTCTTCTT 22783 0.15385626274270955 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 22125 0.14941271181066798 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 22031 0.14877791882037633 No Hit
ACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGACGG 20902 0.1411536498199585 No Hit
ACCTCCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCC 20318 0.1372098295398487 No Hit
CAAGGTGAAACAGGTGAAATTAAAGGGCATTATCTAAATGCTACTGCAGG 19778 0.1335631464041307 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 18956 0.12801208429753774 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 18944 0.12793104689452178 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 18653 0.12596588987138488 No Hit
ATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGGAT 18208 0.12296075284287651 No Hit
GCTAAAAACTATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGA 18201 0.12291348102445054 No Hit
CCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGATT 17732 0.11974626918991027 No Hit
ACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGACTG 17561 0.11859148619693292 No Hit
GCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCT 17547 0.11849694256008098 No Hit
ACCAATAGGGGTGCTTCGTGCACCCCCAGAAGCATGTGTCGAGAGCTCCC 17339 0.11709229424113772 No Hit
GCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGT 17275 0.11666009475838597 No Hit
TACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAAC 17249 0.11648451371851806 No Hit
TTCATGCGTTGGAGAGACCGTTTCTTATTCTGTGCAGAAGCTATTTACAA 16002 0.10806337692177669 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 15901 0.10738131211305907 No Hit
GGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGGATCG 15847 0.10701664379948726 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 5600840 3.8482034 6.1727905 45-49
CTGCT 4684655 3.5483973 11.42426 85-89
ACTTC 4611905 3.3906496 7.028018 60-64
AACTT 5378410 3.2339096 6.142069 60-64
AGCTG 3884820 2.930749 8.752555 3
TTCAA 4707300 2.830387 8.791943 9
CTTCA 3833150 2.818113 7.172773 60-64
CTACT 3822325 2.8101547 9.450838 85-89
CAACT 3145000 2.7330842 11.35674 9
TCAAC 3085220 2.6811337 11.047409 8
GCTGC 2894485 2.669978 10.753855 3
TGCTA 4308055 2.6687458 6.62122 1
GGAAG 3527945 2.650835 6.3842807 15-19
TCAAG 3559060 2.6060998 5.6119905 10-14
TCTCT 4042105 2.5140877 8.799362 20-24
ACCCA 1980725 2.4877973 43.749065 1
CTTCT 3989545 2.4813967 6.088641 20-24
GCTAC 2770450 2.480474 11.821162 1
CTGGT 3871135 2.4706788 12.500588 1
ACAAC 2380920 2.4457211 8.144735 85-89
CAAGC 2292730 2.426426 7.9791646 75-79
ACTGC 2639230 2.3629885 6.481601 85-89
AGGAA 3239585 2.3626437 6.448537 10-14
TCCTT 3775510 2.3482723 9.827737 40-44
TATGC 3788345 2.346797 8.198617 30-34
AAGCT 3183380 2.3310106 5.8410697 15-19
GGTGG 3526245 2.3093812 8.685674 7
TGGTG 4277480 2.3003259 8.5657215 6
CAACA 2237710 2.2986133 7.796175 85-89
CAGCA 2153315 2.278881 7.340175 2
ACATC 2620945 2.277667 7.0485573 90-94
GAAGC 2536580 2.2619658 7.205028 15-19
CATGG 2980620 2.248611 22.989656 2
CTGAA 3041755 2.2273064 6.2570853 80-84
CGGTG 2861435 2.2240453 14.260567 9
CAGCT 2482485 2.2226496 7.986733 65-69
GGTGT 4121040 2.2161963 12.999774 1
CCAAG 2058835 2.1788917 7.782026 75-79
GCAGC 1961515 2.1387386 7.956174 1
CAAGG 2370590 2.1139464 6.7957134 10-14
GCTCC 1930820 2.1137557 9.113928 65-69
CTTGG 3282175 2.0947864 7.3653893 2
TGAAA 3459370 2.071687 5.034426 65-69
CTGTT 3924850 2.0569289 5.129098 55-59
GCTGG 2579980 2.0052848 10.003546 4
CGTGA 2657090 2.0045366 20.57355 9
TCTGC 2644105 2.0027804 5.6693296 85-89
CTCTC 2222570 1.9979594 5.9222674 15-19
TATTC 3920280 1.9941661 8.64522 5
CTCCT 2218050 1.9938964 10.137922 70-74
GTTCC 2605205 1.9733155 5.18044 70-74
TAGCT 3176130 1.967543 6.5956097 2
CCTTA 2673650 1.9656545 8.59021 95-97
TGGCT 3069080 1.9587824 6.425709 9
AGCTC 2187665 1.9586878 7.7444057 65-69
CACAA 1890160 1.9416041 7.374873 85-89
TTCCA 2634015 1.936515 5.9290385 75-79
TTTCA 3780175 1.9228976 7.5751386 8
TGCTG 3010995 1.9217107 5.3524075 85-89
CCCAA 1506490 1.8921564 44.32702 2
AAGGA 2578640 1.8806136 6.254441 10-14
GCTTC 2477455 1.876551 8.267628 15-19
CTCTG 2471150 1.8717754 7.1096716 20-24
CCAAT 2101880 1.8265866 30.919914 3
GGCTC 1974515 1.8213643 5.554082 10-14
CATCC 1712860 1.820048 7.4786515 90-94
CCAGC 1402295 1.8146052 8.086544 1
CCGTG 1963140 1.8108716 5.93749 60-64
TACTG 2898415 1.7955048 5.323678 85-89
GCTGT 2783060 1.7762356 5.435635 90-94
CACCC 1155975 1.775284 8.456159 50-54
CTCTA 2408350 1.7706072 6.6532607 95-97
GTTGC 2767005 1.7659887 6.224045 75-79
GATCG 2340720 1.7658638 6.6356697 50-54
GGTAT 3380220 1.764389 5.3876586 30-34
CTACC 1654780 1.7583334 7.8956695 25-29
TTCAG 2803300 1.736583 5.994561 65-69
CTTTC 2773770 1.7252152 9.064139 7
CCTGT 2253085 1.7066017 7.288053 4
GTGGC 2182425 1.6962861 7.360962 8
GCACC 1302600 1.6855973 7.3169665 50-54
GCTCT 2202115 1.6679945 5.167508 10-14
GCAGG 1800385 1.6540737 5.427708 90-94
GAACA 1909770 1.652976 6.6490455 80-84
TTCGG 2589155 1.6524794 11.601003 7
CCACA 1312665 1.6487116 7.183584 60-64
ACACA 1585130 1.6282722 7.135325 85-89
TCGGT 2548515 1.6265416 13.525836 8
TTCTT 3770330 1.6225342 5.571853 95-97
ACATG 2197060 1.6087836 22.91646 1
GGAGG 2077260 1.6080655 5.198324 9
TCCAA 1839590 1.59865 6.2541537 75-79
AATGA 2656350 1.5907885 5.8244343 75-79
TCAGC 1756530 1.5726784 6.321274 65-69
TTGGT 3557755 1.5710702 16.35985 7
GCGGC 1395775 1.5679548 7.9709578 75-79
TGAAC 2134730 1.5631429 5.9557905 80-84
GTGTA 2986830 1.5590495 12.292282 2
CAACC 1232465 1.54798 5.205433 75-79
AACAC 1503675 1.5446002 7.1397095 85-89
GCTTG 2413855 1.5405974 7.306219 1
GCTGA 2037695 1.5372585 5.5855775 80-84
TTAGG 2943645 1.536508 6.4049544 30-34
TACTT 3017890 1.5351387 8.725638 3
GCCTT 2013230 1.5249233 5.2509756 35-39
TGTAG 2890575 1.5088067 6.1024656 9
GGCTG 1928905 1.4992379 6.6822124 80-84
ACGGT 1979905 1.4936609 6.544294 4
GGGAA 1984415 1.4910539 5.8130007 15-19
CATGC 1665190 1.4908988 5.812068 2
GGAAC 1665830 1.4854847 6.6618733 15-19
ACTAC 1702490 1.4795066 5.4532237 70-74
GTAGC 1931335 1.4570194 7.5148487 1
AACAT 2040790 1.4504477 5.0916934 90-94
TGCAC 1617715 1.448393 5.2452106 50-54
TGGGA 2273320 1.4450794 6.097292 15-19
GCAAG 1616425 1.4414283 5.354718 1
AACCA 1392700 1.4306049 5.3558836 75-79
ATGCC 1579820 1.4144642 7.0694704 9
TCCTG 1867110 1.4142445 5.621538 70-74
CGCAG 1296300 1.4134215 6.3131666 90-94
CCGCC 890885 1.4095907 5.8912673 45-49
AATCC 1617315 1.4054875 5.3006268 60-64
GAACT 1919035 1.4052017 5.4817066 15-19
CATGA 1917180 1.4038433 5.178422 65-69
AGGTA 2267075 1.3987658 6.4192233 35-39
GCAAC 1320655 1.3976662 5.708777 1
TGGTA 2666830 1.3920176 9.038286 2
ACTTT 2735475 1.39148 8.909415 4
ATGGG 2166805 1.377371 19.88865 3
CAATT 2247660 1.3514643 21.380281 4
GGTAC 1779580 1.3425338 11.375321 3
CTGTA 2165435 1.3414396 7.0612407 8
CACAT 1538975 1.337408 5.0428734 60-64
GGTCG 1708460 1.3278977 20.960709 6
AGCTA 1807965 1.3238714 5.7743835 20-24
GTCAA 1807165 1.3232856 5.30554 10-14
GTATT 3072270 1.3168217 7.7105966 4
ATCTC 1761555 1.2950866 5.70701 45-49
TAGGT 2456895 1.2824367 5.303618 30-34
GTCTG 2004160 1.2791173 5.1335793 90-94
GACGG 1385750 1.2731347 8.450036 3
TTCTC 2033800 1.2649724 5.3054366 20-24
CAGGT 1668170 1.2584848 5.580696 95-97
CGCTG 1354155 1.2491217 6.772663 50-54
TGGTC 1953630 1.2468677 19.935762 8
TGGGT 2301110 1.2374816 15.111346 4
ATCCT 1666820 1.2254379 5.177669 90-94
GAATC 1673075 1.2250988 5.170282 40-44
GTGAG 1918305 1.2194071 6.0399747 10-14
GGTCA 1608365 1.2133676 23.347057 9
GGGTC 1558095 1.2110265 20.920753 5
GTGGG 1847730 1.2101011 5.794379 10-14
GTACT 1939600 1.2015398 9.265951 4
AAGCC 1134610 1.2007725 6.524144 2
CCTTG 1584400 1.2001055 6.526086 45-49
ATTCG 1932670 1.1972469 10.420441 6
TCGCT 1570680 1.1897134 5.4857955 50-54
TGTAT 2769610 1.187097 7.7135067 3
TGCGG 1524140 1.1846352 5.5951667 75-79
TCGTG 1846780 1.1786726 17.314222 8
TGCCT 1554005 1.1770829 5.187946 30-34
AACGC 1110825 1.1756005 5.450023 95-97
CTGAC 1295250 1.1596795 7.692171 1
AGTGG 1820730 1.1573818 5.1446476 10-14
TGGCC 1247355 1.1506056 11.315584 1
AGCCT 1280450 1.1464286 5.5749726 4
ACTTA 1893445 1.1384833 5.3002143 20-24
GCCTG 1231410 1.1358973 8.453674 6
TGACG 1485595 1.1207485 6.5981073 2
CGGCT 1204580 1.1111484 6.5613966 75-79
GCTCA 1238540 1.1089052 6.368319 1
CGTCC 996120 1.0904975 7.3736014 40-44
GAGTG 1707880 1.0856465 5.2503824 10-14
CCGTT 1433275 1.0856358 5.5627656 25-29
GTCCT 1430090 1.0832233 5.4307013 40-44
ATTGG 2067160 1.079005 16.963722 6
GGATC 1430020 1.0788221 5.724895 45-49
ACCGT 1203410 1.0774522 6.1826925 25-29
TCTTG 2050070 1.0743973 6.1082997 95-97
CTTAT 2059645 1.0476991 5.6929445 95-97
CCAGG 956810 1.0432583 5.3514304 1
TTGGC 1632680 1.0420272 5.8595977 3
TACAT 1684905 1.0130932 5.940199 4
AATTG 1986960 1.0066684 15.920583 5
GCGTC 1070865 0.9878048 6.1673927 40-44
ATCGC 1101170 0.9859134 6.0708694 50-54
ATGCG 1281865 0.9670524 5.458845 35-39
TACCG 1071675 0.95950556 6.0388894 25-29
GGCCA 833215 0.90849644 5.348782 1
CGGTA 1195640 0.90200335 6.1034474 5
TGCGT 1411780 0.901042 5.3085284 40-44
GCCCA 686125 0.8878631 5.1941113 2
GTCGT 1365975 0.8718078 17.073423 7
CTACA 999620 0.8686949 8.922521 3
GCATA 1177440 0.8621732 5.9584184 60-64
GGCCT 924270 0.8525802 8.133386 5
GGCCC 555335 0.7403717 6.2104597 1
GCCCT 652295 0.7140967 5.709077 1
CCCCG 449735 0.7115871 5.876464 1
GGTAA 905160 0.5584759 5.8079357 7