FastQCFastQC Report
Mon 21 Jul 2014
1L15C_ACAGTG_L004_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 1L15C_ACAGTG_L004_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 15326330
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[WARN] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 162413 1.059699223493165 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 99729 0.6507037235920146 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 97982 0.6393050391059047 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 84564 0.551756356544587 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 63418 0.4137846438123151 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 61810 0.40329289529848306 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 60412 0.39417133782190517 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 59305 0.3869484736398081 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 59189 0.3861916062097058 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 58089 0.37901441506218386 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 47128 0.30749696763674017 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 46369 0.30254470574495 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 45953 0.29983042254734177 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 37495 0.244644347342123 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 36076 0.2353857707618197 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 34533 0.2253181289976139 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 33857 0.22090741880150042 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 33506 0.2186172423535184 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 33506 0.2186172423535184 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 33483 0.21846717381134298 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 33129 0.21615742320568587 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 32259 0.21048091747991857 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 30811 0.20103312404208964 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 30466 0.19878209590945775 No Hit
GGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCAT 27051 0.17650017975601465 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 26287 0.1715152942680994 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 25666 0.167463443629362 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 25444 0.16601495596140758 No Hit
CCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCCAGAA 25425 0.1658909862961322 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 24400 0.15920314909048675 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 24015 0.15669113218885408 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 23709 0.15469456810599796 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 23653 0.15432918382939687 No Hit
ATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAA 23433 0.15289374559989247 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 23206 0.15141263433581295 No Hit
ACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGACTG 23140 0.15098200286696162 No Hit
TACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGA 21369 0.13942672511945128 No Hit
GCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATG 20877 0.1362165632607415 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 20419 0.13322824185568236 No Hit
GTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGG 19946 0.1301420496622479 No Hit
GGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTA 19652 0.12822378221009204 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 19569 0.12768223051441538 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 19264 0.1256921911507843 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 18934 0.12353903380652773 No Hit
CCACGTAGTTGGTTAGCTACTTCTCACTTTGTATTAGGATTTTTCTTCTT 18674 0.12184260680802253 No Hit
GCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGA 18599 0.12135325286614604 No Hit
ATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTG 18165 0.11852152472248736 No Hit
TTCATGCGTTGGAGAGACCGTTTCTTATTCTGTGCAGAAGCTATTTACAA 17857 0.11651191120118125 No Hit
GACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCT 17525 0.11434570441847462 No Hit
CTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGT 17254 0.11257750550849421 No Hit
GCGACTGGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGT 17119 0.11169666841311651 No Hit
GCTCATGGTAAGGCTTTATATGGTTTTGATGTTCTTTTATCATCTGCAGA 16788 0.10953698634963492 No Hit
GGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTATTT 16648 0.10862352565813212 No Hit
ATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCATCTC 16267 0.10613760763339951 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 15906 0.10378218399316733 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 15622 0.10192916373326165 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGCT 4682830 3.4830923 7.210201 5
ACTTC 4704410 3.2190154 5.757379 60-64
AAAAA 5279895 3.1632614 4.431234 45-49
GGAAG 3941275 3.0329313 5.9509506 85-89
AGCAC 2933180 2.988009 6.624913 90-94
GAAGA 4181690 2.9603221 5.901028 90-94
AGCTG 3722035 2.815929 6.7476115 3
CTACT 4112925 2.8142889 8.786019 2
CGGAA 2977050 2.6358497 6.888427 95-97
CTTCT 4487865 2.6240034 5.2561693 40-44
TCTCT 4463200 2.609582 7.3012466 20-24
CTGGT 4008990 2.591689 11.340218 1
GCTGC 2703635 2.5582125 7.0241156 3
CTTCA 3718390 2.5443268 5.778882 60-64
CACAC 2082040 2.4402874 10.6325 95-97
TTCAA 4459975 2.440074 6.627928 9
CTGAA 3462810 2.4100783 5.1248627 80-84
TGGTG 4262925 2.3952281 8.102552 6
TCTGC 3175450 2.361902 5.5003133 85-89
CGGTG 2862730 2.354292 10.647252 9
AGAGC 2654270 2.3500636 6.669008 95-97
GGTGT 4181030 2.3492136 8.945662 7
GCAGC 2120160 2.3477428 5.7006874 1
GGTGG 3274870 2.3408053 9.835588 7
CAACT 2917430 2.3362088 7.2694726 9
ACAAC 2450195 2.296176 5.2326374 85-89
TCGGA 3034785 2.2959855 5.6463733 95-97
CCGTG 2426020 2.2955296 5.7383556 60-64
ATCGG 3015605 2.2814748 5.886944 95-97
AAGAG 3190220 2.2584362 5.230136 90-94
CAACA 2406320 2.255059 5.092006 85-89
CAAGC 2206890 2.2481427 5.989371 2
CAGCA 2206000 2.247236 5.4870048 2
TATGC 3708965 2.205779 5.8594847 30-34
GAGCA 2487010 2.2019732 6.2293706 90-94
ATGCA 3144845 2.1887784 6.265494 1
CATGG 2885770 2.1832473 8.583366 2
GAAGC 2454910 2.1735523 5.063483 15-19
ATGGT 4197535 2.1696758 5.0339394 1
GATCG 2849765 2.1560075 6.489532 95-97
ACCCA 1834445 2.1500895 23.893898 1
CGTGA 2827570 2.1392155 6.6658993 9
GCACA 2099085 2.1383224 6.8729043 95-97
ACTGC 2412795 2.1002467 7.011355 4
GCTGG 2539945 2.0888355 7.7698793 4
TCAAC 2561900 2.051509 6.0082555 8
CCAAG 2003740 2.0411954 5.231277 75-79
TCCTT 3476925 2.0329182 5.3931108 40-44
AGATC 2854135 1.9864471 6.0272737 95-97
CTCTG 2593025 1.9286939 5.5855603 45-49
CTCCT 2235475 1.9130887 5.222808 70-74
CCAGC 1500525 1.9117619 6.2276564 1
CTTGG 2929430 1.8937867 5.5297155 2
TGCTG 2919870 1.8876063 5.4268947 6
GCTAC 2165560 1.8850379 10.841525 1
GGCTC 1978670 1.8722419 5.1335745 10-14
CCTTA 2703415 1.8498249 5.669489 45-49
TGGCT 2840035 1.8359956 7.629802 9
TATTC 3880840 1.8142747 6.035185 2
CATCC 1789545 1.7922606 5.2500777 90-94
GCTTC 2394740 1.7812092 6.230901 15-19
GCATG 2342730 1.7724068 5.434994 3
TTCGG 2732930 1.7667555 8.255196 7
GCAGG 1828930 1.7602334 5.0399942 90-94
GTGGC 2138445 1.7586442 8.9456215 8
TTTCA 3752595 1.7543206 5.8602653 8
CACCC 1178280 1.7272203 5.7670116 50-54
CCTGT 2319350 1.7251343 7.4674177 1
ACACA 1815035 1.7009422 5.554627 85-89
TCGGT 2619495 1.6934233 9.193756 8
AATGA 3014570 1.6775697 5.1134934 75-79
CACAA 1782290 1.6702555 5.288581 4
GCACC 1309965 1.6689764 5.23552 50-54
GCTCT 2240650 1.6665971 5.0759683 10-14
GTCTG 2538260 1.6409072 5.902946 90-94
TTGGT 3702890 1.6354907 11.113783 7
CCGCC 1005130 1.6016225 6.292138 45-49
CTTTC 2709055 1.5839536 6.921064 7
TGTAG 3061555 1.5824959 5.8828588 3
CCTCC 1260390 1.5787411 5.0025325 1
CGCAG 1422285 1.574956 5.8692775 90-94
CCACA 1342115 1.5730466 6.112526 1
CCCAA 1324975 1.5529574 23.592926 2
GTGTA 2979810 1.5402426 8.062707 2
CATGC 1762390 1.5340939 5.2229548 2
TGGTA 2955865 1.5278655 8.486546 2
ACACG 1498255 1.5262616 6.6924167 95-97
GCTTG 2358920 1.5249693 5.2238636 1
TACTT 3204440 1.4980607 6.2917976 5
GTAGC 1977605 1.4961693 5.483934 1
GCAAC 1467370 1.4947991 5.050245 1
AGCCT 1689560 1.4706981 5.9118075 2
CCAAT 1802705 1.4435635 16.489552 3
GCTGT 2165305 1.3998033 5.558323 7
ATGCC 1604380 1.3965521 5.9144273 9
CACGT 1575370 1.3713 5.158686 95-97
GTATT 3362300 1.3661708 5.255684 4
ACATG 1956770 1.3618909 7.794885 1
ACTTT 2837605 1.326567 6.1206465 6
GGTCA 1712155 1.2953415 14.751558 9
ACGGT 1703060 1.2884607 5.728114 4
GCGGC 1056410 1.2716074 5.0407577 7
ATGGG 1927340 1.2673334 6.9732876 3
GGTAC 1652125 1.2499254 9.414298 3
TGGTC 1921385 1.2421165 12.849566 8
AAGCC 1215870 1.238598 5.909331 1
TGTAT 2987405 1.2138435 5.055702 3
GACGG 1253525 1.2064414 7.1501737 3
GGTCG 1447960 1.1907935 6.341041 6
TGGGT 2111975 1.1866647 5.231145 4
CAATT 2167980 1.1861124 11.343957 4
ATTCG 1951070 1.1603316 6.573051 6
GTACT 1817135 1.0806781 7.3389874 4
CTGAC 1237190 1.0769271 6.635702 1
GGGTC 1302200 1.0709214 6.779004 9
ATTGG 2059265 1.0644195 10.483675 6
TGACG 1403265 1.0616488 5.619157 2
TGGGG 1459870 1.043483 5.202183 8
TGGCC 1090160 1.0315228 7.142262 1
TCGTG 1522870 0.98448867 5.011926 8
GGGGT 1344575 0.96107274 5.146033 8
AATTG 1942150 0.9235166 9.006613 5
CGGTA 1127320 0.8528809 5.2310214 5
GCCTA 931655 0.81097037 5.7172227 3
CCCCG 414135 0.65990263 5.6529655 1
Produced by FastQC (version 0.10.1)