FastQCFastQC Report
Mon 21 Jul 2014
1L15B_ACAGTG_L003_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 1L15B_ACAGTG_L003_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 14966965
Filtered Sequences 0
Sequence length 101
%GC 45

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 181344 1.211628409634151 No Hit
GCGAGTCGGGCTGTTTGGGAATGCAGCCCTAAGTGGGAGGTAAATTCCTT 158409 1.0583909296240086 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 123975 0.8283242460979898 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 117139 0.7826503235625927 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 109399 0.7309364323361484 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 106411 0.7109724650254745 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 85915 0.5740308739948279 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 74022 0.49456920624856143 No Hit
CCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCCAGAA 68219 0.455797150591319 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 67790 0.45293083801558964 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 66114 0.44173284296448884 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 61517 0.4110185331495063 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 59527 0.39772258437164787 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 51982 0.3473115624978077 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 47371 0.31650371334468946 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 45269 0.30245944986174556 No Hit
GATACCGTCCTAGTCTCAACCATAAACGATGCCGACTAGGGATTGGCGGA 44913 0.30008087812058093 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 44414 0.2967468688541732 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 41174 0.2750991934570569 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 40354 0.2696204607948238 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 39321 0.26271859391666913 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 39012 0.2606540470963886 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 36033 0.2407502122173734 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 35212 0.23526479817384485 No Hit
ACCTCCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCC 35125 0.2346835180011445 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 34566 0.23094862585701245 No Hit
ACCAATAGGGGTGCTTCGTGCACCCCCAGAAGCATGTGTCGAGAGCTCCC 32241 0.2154144143451929 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 31557 0.210844349539135 No Hit
GGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTATTT 30732 0.20533220997042487 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 30169 0.20157059230111116 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 29821 0.19924547161030975 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 29604 0.1977956118692066 No Hit
GATCAGGAAAGACTACCCGCTGAGTTTAAGCATATCACTAAGCGGAGGAG 28901 0.19309860081853603 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 28744 0.19204962395515723 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 27583 0.1842925402711906 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 27007 0.1804440646450366 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 26378 0.17624147581022606 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 26053 0.17407002688921902 No Hit
GGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTA 25535 0.17060907137819858 No Hit
ATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCATCTC 24498 0.1636804789748623 No Hit
GTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCATCTCTGCT 23398 0.15633095954991544 No Hit
GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA 23116 0.1544468100246109 No Hit
AAGTGGGAGGTAAATTCCTTCTAAGGCTAAATATTGGCAAGAGACCGATA 22865 0.1527697833194639 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 22605 0.15103262418265828 No Hit
ATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGATTTCACTAAA 22141 0.14793246326158976 No Hit
GGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCAT 21653 0.14467194918943152 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 21437 0.1432287708296238 No Hit
GTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAG 21330 0.14251386303101532 No Hit
TGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGT 20655 0.1380039306566161 No Hit
TACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGA 20614 0.13772999402350444 No Hit
GTATGGGCTTGGATGTTCTTATTTGGACATCTTGTTTGGGCTACTGGGTT 20082 0.1341754991743483 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 19318 0.12907092386465793 No Hit
GTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGG 19148 0.12793508904443887 No Hit
GCTCATGGTAAGGCTTTATATGGTTTTGATGTTCTTTTATCATCTGCAGA 18900 0.1262781064831781 No Hit
GTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCTTTCAA 18868 0.12606430228172513 No Hit
CGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTC 18638 0.12452758458378167 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 18525 0.12377258849740078 No Hit
GCGACTGGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGT 17915 0.11969694590720298 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 17456 0.11663019189261149 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 17038 0.11383737451113168 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 16813 0.11233406371966527 No Hit
GGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCTGGTACTTT 16789 0.11217371056857553 No Hit
CAACACGGGGAAACTTACCAGGTCCAGACATAGTAAGGATTGACAGATTG 16727 0.11175946492826033 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 16598 0.11089756674115293 No Hit
GGTCCTTACGAGTTAATCGTTCTTCACTTCTTACTTGGTGTAGCTTGCTA 16357 0.10928735384896002 No Hit
CTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGT 15610 0.10429636202129156 No Hit
TGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTATT 15393 0.10284650228018839 No Hit
TGGGTCGGGGGGAGCGGTCCGCCCCTTGTGGGTGTGCACTGGTCCACTCG 15018 0.10034098429441106 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
ACTTC 4498740 3.3269103 5.747678 60-64
CTGCT 4188050 3.2116287 12.157616 5
AACTT 5122075 3.0679915 4.944242 55-59
AAAAA 4574940 2.9275646 5.0136514 45-49
CTTCT 4405990 2.8370454 7.7573075 40-44
CTACT 3704570 2.7396057 12.341451 2
CAACT 3163735 2.687062 7.025551 9
TTCAA 4319925 2.5875242 7.4373155 9
TCTCT 4000945 2.5762343 7.6974244 20-24
AGCAC 2490685 2.5193412 5.2023716 90-94
CTTCA 3403640 2.5170615 5.6399403 60-64
TCAAC 2936300 2.4938943 6.3706512 8
CTGGT 3861570 2.4871309 11.686431 1
GCAGC 2310620 2.4235945 7.3351464 1
AGCTG 3247885 2.4024963 7.431243 3
CGGAA 2670505 2.2687304 5.5040064 95-97
ACTGC 2548065 2.244147 12.087915 4
GCTGC 2443965 2.232025 6.50165 3
GGTTC 3420435 2.2030077 7.9862313 3
TGGTG 4028320 2.179114 7.9129467 6
TCTGC 2833690 2.1730304 5.1394167 85-89
ACCCA 1803880 2.172474 26.10769 1
GGTGG 3363300 2.166767 8.165852 7
TCCTT 3328485 2.1432333 7.389979 45-49
CAGCA 2112180 2.1364818 5.2873755 2
TATGC 3407725 2.1165867 5.958784 30-34
CATGG 2853600 2.1108394 9.501416 2
CGGTG 2737475 2.0997882 11.968618 9
CTGCA 2374350 2.091152 5.3905 35-39
ACAAC 2135655 2.0832245 5.065703 85-89
GCTCC 1910605 2.0775604 7.634826 70-74
ATGGT 3964055 2.0679138 8.833244 1
GGTGT 3811575 2.061866 9.62559 1
TGCAG 2759760 2.041425 5.669716 65-69
CCAAG 2012420 2.0355737 5.1956086 75-79
ATGCA 2852040 2.0344853 5.1451797 1
CAACA 2079575 2.0285213 5.0324917 85-89
TTTCA 3888220 2.0278304 6.848786 8
CTCTG 2622940 2.0114155 6.9065623 45-49
CTCCT 2200910 2.0095298 9.117002 70-74
CAAGG 2361780 2.0064528 5.0472465 4
CACAC 1663990 2.0039997 8.483442 95-97
ACATC 2339380 1.9869108 5.0377216 40-44
TGGTT 4292895 1.9499147 5.8735466 2
CAAGC 1924890 1.9470366 5.2417345 75-79
AGCTC 2209510 1.9459729 5.713655 70-74
CAGCT 2203345 1.9405433 6.2076955 65-69
CCGTG 2113175 1.929921 5.4144278 95-97
TTCCT 2955740 1.90322 6.07672 45-49
CTTTC 2911195 1.8745372 7.854688 7
GCTAC 2122870 1.8696668 14.815152 1
CTGTT 3411505 1.8449733 7.713545 8
GATCG 2471005 1.8278297 5.2275767 95-97
CGTGA 2465825 1.823998 7.693944 9
GCTTC 2332580 1.7887512 6.2362638 15-19
GCACA 1753135 1.7733057 5.4495792 95-97
CTCTA 2397365 1.7729005 6.5682898 25-29
GCACC 1418980 1.7720927 5.4059243 50-54
GCTGG 2308840 1.7710024 8.980697 4
TGCTG 2741965 1.766024 9.083168 6
CCAGC 1410565 1.7615837 5.6138625 1
CCTTA 2377765 1.7584058 5.7847385 45-49
AGATC 2458860 1.7540121 5.3043013 95-97
CTTGG 2722070 1.7532102 6.515108 2
CACCC 1178215 1.7519166 5.9603963 50-54
GGAGA 2452460 1.7498978 5.5499387 8
ATCCG 1972140 1.7369151 6.325518 95-97
GAACA 2110110 1.7287467 7.627749 80-84
CTACC 1636480 1.7160513 6.5741034 20-24
CCTGT 2237575 1.7158962 8.233026 1
CCCAA 1417265 1.7068605 27.381413 2
CCAAT 1999755 1.6984563 19.078297 3
TGGCT 2621885 1.6886837 5.9623575 9
TATTC 3233315 1.6862768 6.650902 5
CATCC 1594365 1.6718884 5.0444403 90-94
CCTAC 1589970 1.6672797 5.327162 20-24
TCCGT 2156280 1.6535549 5.379857 95-97
GGAGG 2213195 1.6375467 5.4245243 6
TACTG 2620270 1.6274871 8.700964 3
AATGA 2813110 1.625338 5.082894 75-79
TAGCT 2588170 1.6075493 5.615401 2
TGGTA 3075615 1.6044446 9.146239 2
CCTCC 1235375 1.5994117 6.241443 2
TTCGG 2479705 1.5971096 9.843535 7
ATGAT 3092425 1.5557102 5.139997 15-19
TTCAT 2967225 1.5475022 5.417983 60-64
TACTT 2956190 1.5417472 6.950353 5
ACACA 1568530 1.5300225 5.326851 85-89
GCTGT 2371105 1.5271633 9.257578 7
ATGCC 1732225 1.5256157 6.1025653 9
CTGTA 2454440 1.5244877 6.499844 8
TGTTT 3948000 1.5057464 6.33983 9
TCGGT 2334595 1.5036484 11.325013 8
TTGGT 3298655 1.498312 10.840595 7
ACTTT 2863270 1.4932864 6.879281 6
CCACA 1232760 1.4846548 5.6599774 60-64
GTCTG 2302115 1.4827287 5.6520557 90-94
CCGCC 956625 1.4750063 6.211636 45-49
TGTAG 2826180 1.4743227 5.950342 3
GCAAC 1454990 1.4717302 5.5005517 1
GCTTG 2273160 1.4640796 6.2344103 1
GTGGC 1841355 1.4124167 6.4929986 8
GGCTG 1798700 1.379698 17.17455 9
GCGGC 1264230 1.3750571 5.7762713 7
ACGGT 1856110 1.3729851 5.6103806 4
AGCCT 1526525 1.3444504 6.24427 2
GTGTT 2958605 1.3438548 6.0886254 9
ACATG 1882000 1.3425131 8.791499 1
GTGTA 2568305 1.3397981 9.048336 2
ACACG 1312495 1.3275958 5.265767 95-97
GTATT 3026005 1.3254757 6.0119133 4
GACGG 1469505 1.2945648 6.7525554 3
AGGTA 2145685 1.2855414 5.106829 35-39
GTAGC 1737580 1.2853069 6.4584794 1
GGTAC 1726415 1.2770481 9.963088 3
CGCAG 1211545 1.2707818 5.051235 90-94
GCCTG 1377290 1.2578517 7.185915 6
AACAC 1284850 1.2533069 5.5411983 2
CAATT 2079125 1.2453425 13.228597 4
TGGTC 1909840 1.2300752 12.974819 8
CCCTA 1163560 1.2201362 5.197134 2
CAGGA 1434690 1.2188424 5.697773 4
GAGTC 1635570 1.2098491 12.328487 3
ATTGG 2306510 1.2032285 10.951248 6
GTACT 1895585 1.1773748 8.173551 4
GGTCA 1582480 1.170578 14.743617 9
TGTAT 2649000 1.1603369 5.8451633 3
GCGAG 1309730 1.1538105 15.368376 1
TTGGC 1781495 1.1474118 5.1992183 3
ATGGG 1841115 1.1438375 8.398607 3
TGACG 1531665 1.1329895 5.3607116 2
GTTAC 1802880 1.1197944 6.1389923 9
AGCCC 890025 1.1115074 5.2616696 25-29
AAGCC 1098385 1.1110225 6.4153457 1
CTGAC 1245845 1.0972482 6.308835 1
TGGGT 2004225 1.0841826 6.063516 4
GGTCG 1359340 1.0426857 7.635989 6
GCCCT 954660 1.0380818 6.057362 1
ATTCG 1666575 1.0351335 7.7717385 6
TGGCC 1132955 1.0347054 8.59983 1
TCGTG 1596970 1.0285643 6.254088 8
GTTCG 1561150 1.0054936 7.614234 4
CTCCC 770350 0.9973546 5.3781524 3
AATTG 1961705 0.98687744 10.13786 5
GTAGA 1619760 0.9704447 5.0554852 4
TACCG 1074745 0.946556 5.349913 3
GCCCA 739615 0.923668 5.3530354 1
CCCCG 592535 0.9136212 12.232631 1
GGGTC 1189525 0.9124286 7.4849677 5
TGGGG 1403050 0.90389866 5.28718 8
GCCAG 856960 0.89885986 5.3241115 2
CGGTA 1199255 0.8871022 5.0432444 5
TCCCC 677800 0.87753224 5.146476 4
GGGCT 1123010 0.86140805 13.634367 8
GGGGT 1313760 0.84637463 6.6924706 8
GGCCT 902160 0.8239249 7.045078 5
CGTCC 755965 0.8220239 5.360758 6
GGTTG 1498925 0.8108414 5.6322446 5
CGGTT 1161485 0.74808043 5.025557 4
CGAGT 997885 0.73814654 12.497805 2
GGCCC 567735 0.735223 6.3189044 1
GCCTA 833130 0.7337593 5.8709803 3
CACGG 689530 0.7232436 5.891264 4
CCCGG 526940 0.682393 10.443187 2
CGGGC 606990 0.660201 18.603697 7
GTCGT 1022890 0.6588152 6.2606325 7
TCGGG 843990 0.64738506 13.3083 6
CCGGT 657485 0.6004681 7.274053 3
AGTCG 811625 0.60036796 12.530089 4
CCGTC 551650 0.5998552 5.633385 5
GTCGG 765010 0.58680314 13.220889 5
Produced by FastQC (version 0.10.1)