FastQCFastQC Report
Mon 21 Jul 2014
1L15A_ACAGTG_L002_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 1L15A_ACAGTG_L002_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 13114959
Filtered Sequences 0
Sequence length 101
%GC 45

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 192951 1.471228388895459 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 133057 1.0145437740217107 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 117827 0.8984168383599217 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 77854 0.5936274753127326 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 77478 0.590760520105324 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 70599 0.5383089645953144 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 69234 0.5279010022067168 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 59468 0.4534364156228014 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 56122 0.42792356422921335 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 55367 0.4221667791717839 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 52295 0.3987431451367862 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 43783 0.33384015916481324 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 43161 0.3290974832631959 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 42066 0.3207482387097055 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 41090 0.3133063549798364 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 39872 0.3040192500792416 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 39773 0.3032643868730356 No Hit
CCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCCAGAA 36370 0.27731691726981383 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 35726 0.27240649398903954 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 35248 0.2687618009328127 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 32453 0.2474502588990175 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 31677 0.24153335134330195 No Hit
GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA 31611 0.2410301092058313 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 29969 0.22851005481603104 No Hit
TACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAAC 28581 0.2179267201674058 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 28305 0.21582225304707395 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 28002 0.21351191414323142 No Hit
ACCCAATTTGGGAAGCTGCTTCCGTTGATGAATGGCTTTACAACGGTGGT 27236 0.2076712553962235 No Hit
ACCTCCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCC 25915 0.19759878776593964 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 25879 0.19732429205459198 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 25815 0.1968362996788629 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 25039 0.19091939212314732 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 25028 0.19083551843356886 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 24477 0.18663420907377598 No Hit
GCTCATGGTAAGGCTTTATATGGTTTTGATGTTCTTTTATCATCTGCAGA 23750 0.18109092068072802 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 23536 0.17945919617438377 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 22932 0.1748537681284402 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 22529 0.1717809411375209 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 22105 0.1685479916483155 No Hit
TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC 21262 0.16212021707425847 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 20215 0.15413696680256492 No Hit
GTATGGGCTTGGATGTTCTTATTTGGACATCTTGTTTGGGCTACTGGGTT 19792 0.15091164219423028 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 19125 0.14582584665342835 No Hit
GGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTA 18379 0.1401376855238358 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 17903 0.13650824222935048 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 16953 0.12926460540212134 No Hit
GGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTATTT 16438 0.1253377917536761 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 15566 0.11868889563436684 No Hit
ACCAATAGGGGTGCTTCGTGCACCCCCAGAAGCATGTGTCGAGAGCTCCC 15330 0.1168894237488657 No Hit
GCGACTGGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGT 15127 0.1153415729320999 No Hit
GTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAG 14658 0.11176550380370995 No Hit
CAAGGTGAAACAGGTGAAATTAAAGGGCATTATCTAAATGCTACTGCAGG 14507 0.11061414679222406 No Hit
ATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGATTTCACTAAA 14507 0.11061414679222406 No Hit
CATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGC 14507 0.11061414679222406 No Hit
GGTCCTTACGAGTTAATCGTTCTTCACTTCTTACTTGGTGTAGCTTGCTA 14217 0.10840293133970147 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 13987 0.10664920873942496 No Hit
GACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCT 13723 0.10463624018954233 No Hit
GATGAAATTAGCAGTATTATCCGTAAACAGATAGAAGACTATAGTCAAGA 13593 0.10364500567634258 No Hit
ATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCATCTC 13516 0.10305788984929347 No Hit
CAGCTCCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGT 13318 0.1015481634368815 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 4876155 3.7582765 5.7492294 45-49
ACTTC 3961925 3.306014 5.908198 60-64
CTGCT 3775850 3.2434685 8.590943 5
AACTT 4466350 3.0399814 5.170405 60-64
CAACT 2811485 2.764209 7.063704 9
CTACT 3257050 2.7178338 9.443631 2
AGCTG 3138810 2.6675515 7.06362 3
TTCAA 3882095 2.6423132 6.753396 55-59
CTTCA 3166295 2.6421034 6.0732226 60-64
CTTCT 3634720 2.5741434 6.3670893 40-44
GCTGC 2452995 2.5557876 8.16059 3
TCAAC 2568440 2.525251 6.838061 55-59
GGAAG 2963740 2.4919584 5.1897273 15-19
TCTCT 3436060 2.4334507 7.6916924 20-24
AGGAA 2925340 2.3893542 5.4200034 10-14
ACTGC 2358485 2.3870678 8.485363 4
GCAGC 1935720 2.376333 8.884418 1
ACCCA 1663610 2.3626726 34.58391 1
GAAGC 2345440 2.3485987 5.912805 15-19
CTGGT 3245055 2.3406327 9.470314 1
ACAAC 1980965 2.2948196 6.31754 85-89
CAGCA 1887815 2.2512705 6.636154 2
CAAGC 1881380 2.2435966 7.184396 2
CTGCA 2206950 2.2336965 5.1894274 95-97
CAGCT 2190850 2.217401 7.0880117 2
CAACA 1890285 2.1897726 6.2566323 85-89
CTCCT 2126760 2.1756911 9.512543 70-74
TGGTG 3560565 2.1564841 7.089029 6
CAAGG 2145675 2.1485646 5.4129925 10-14
GCTCC 1729285 2.1457465 7.1991825 70-74
GCTAC 2119575 2.1452622 12.013971 1
GGTGG 2892940 2.125201 6.4107394 7
GGTGT 3490035 2.1137671 10.907553 1
CGGTG 2403720 2.1029465 13.28552 9
TATGC 3000405 2.1022992 6.536009 30-34
GGTTC 2898860 2.0909247 6.5053616 3
CCAAG 1733445 2.0671802 6.2756267 75-79
CATGG 2430665 2.065727 14.889333 2
TCCTT 2912650 2.0627666 7.1034784 40-44
ACATC 2068335 2.0335553 5.0454383 90-94
TGCAG 2380440 2.0230427 5.1261287 65-69
AGCTC 1994205 2.0183728 6.124168 65-69
TTTCA 3428715 1.9806734 5.260214 8
CTCTG 2270845 1.9506638 6.426407 20-24
ATGGT 3312695 1.9490024 7.3292184 1
CTTGG 2658890 1.9178364 6.5826883 2
AAGGA 2323695 1.8979437 5.1348944 10-14
TGCTG 2609555 1.8822515 6.382587 6
GCTGG 2149345 1.8804009 8.5706005 4
GCTTC 2179010 1.8717773 7.3252053 15-19
CCCAA 1314425 1.8667574 35.271816 2
CTGTT 3103005 1.8452724 5.4129996 8
CGTGA 2151355 1.8283525 12.739211 9
CCAGC 1235910 1.8069052 7.303985 1
TTCCA 2163665 1.8054625 5.031202 75-79
CTTTC 2540120 1.7989373 6.234905 7
CACAA 1550500 1.7961538 5.875229 85-89
CCAAT 1814945 1.7844262 24.494724 3
CACCC 1011985 1.7620018 5.534634 50-54
TAGCT 2501470 1.7527095 5.0353436 2
CCTGT 2032845 1.746221 7.816864 1
CCTCC 1178360 1.7413003 5.7587886 2
GGAGA 2066270 1.7373519 5.1862016 7
CCTTA 2076420 1.7326612 6.4880977 95-97
CTCTA 2056590 1.7161142 5.7966485 25-29
TATTC 2934150 1.6949772 7.3553634 5
CATCC 1404065 1.6923982 5.970263 90-94
GCACC 1146805 1.6766334 5.105333 1
CTACC 1384345 1.6686286 5.705209 20-24
GCAGG 1606005 1.655495 5.5077953 4
GGAGG 1899245 1.6439106 5.136087 7
TACTG 2340590 1.6399854 5.883098 3
AATGA 2432270 1.6378828 5.2007976 75-79
GCAAC 1365940 1.6289204 5.5041513 1
GCTGT 2240065 1.6157411 6.4952426 7
GAACA 1645135 1.6002562 5.6462107 80-84
ACACA 1368885 1.5857645 5.9745693 85-89
TTCGG 2184150 1.5754102 10.819693 7
ATCCG 1556540 1.5754037 5.8472686 95-97
CCACA 1101455 1.5642954 6.1549044 1
CTGTA 2210105 1.5485585 6.4359593 8
TCGGT 2131055 1.5371132 13.094724 8
TTGGT 3059965 1.5279555 12.781565 7
TGTAG 2584085 1.5203294 5.6686645 9
TACTT 2589565 1.49592 5.629419 5
GCTTG 2054520 1.4819092 6.3979797 1
TCCAA 1507030 1.4816889 5.0332747 75-79
TTCAT 2564655 1.4815301 5.307432 60-64
ACATG 1792205 1.479582 14.598076 1
TGGTA 2505020 1.4738119 7.1020904 2
GCGGC 1163000 1.4697415 7.934619 7
ATGCC 1426260 1.443545 7.646574 9
GGCTG 1649635 1.4432188 6.3551064 9
ACGGT 1698180 1.4432166 7.0677414 4
GCAAG 1429225 1.4311498 5.0604825 1
CCGCC 793350 1.4219805 6.423811 45-49
CACAC 988545 1.4039398 5.005331 95-97
AACCA 1197680 1.3874347 5.0304313 75-79
GTGTA 2343810 1.378965 10.247702 2
AACAC 1189045 1.3774316 5.588896 85-89
GTAGC 1601445 1.3610055 5.786008 1
CAATT 1986875 1.3523487 16.930851 4
ACTTT 2322580 1.3416901 5.593841 6
GGTAC 1543530 1.3117858 8.102207 3
GACGG 1251455 1.2900193 8.992533 3
GTATT 2632850 1.2770969 6.6155944 4
ATGGG 1785405 1.274092 13.00825 3
TGGTC 1720205 1.2407701 15.381296 8
GGTCA 1433310 1.218114 17.879406 9
GTATG 2031075 1.1949697 5.229563 7
AAGCC 1000730 1.1933976 6.97374 1
AGCCT 1178710 1.192995 5.64202 4
GGTCG 1356785 1.1870127 12.899813 6
GCCTG 1137585 1.1852554 7.6325846 6
GTACT 1683000 1.1792307 6.5273376 4
CTGAC 1161345 1.1754194 8.166472 1
TGTAT 2398285 1.1633182 6.525266 3
TGGGT 1906315 1.1545745 9.556207 4
TGGCC 1097910 1.1439178 10.913511 1
TGACG 1337200 1.136434 7.108567 2
TCGTG 1562725 1.1271813 10.664736 8
GCTCA 1103295 1.116666 5.1124644 1
ATTGG 1863285 1.0962514 13.2001095 6
CCAGG 892625 1.0958064 5.0924845 1
TGCGG 1240345 1.0851426 5.773414 6
GGGTC 1226030 1.0726188 12.839991 5
ATTCG 1493160 1.046215 8.871878 6
TTGGC 1429400 1.0310149 5.2233877 3
AATTG 1784210 1.0197186 12.207453 5
CGGCT 964315 1.0047245 6.2446356 8
GTTCG 1363285 0.9833267 6.1804085 4
TGGGG 1321490 0.970788 5.245797 8
GGCCA 769080 0.9441397 5.2546806 1
GCCCA 641415 0.9377512 5.8385854 2
CGGTA 1101635 0.93623644 6.5752378 5
GGGGT 1231090 0.9043788 5.3936424 8
CAGGC 700565 0.8600292 5.2469287 1
AGGCC 699910 0.8592252 5.13118 2
CTACA 863910 0.8493831 5.2861953 3
GGCCT 810260 0.84421384 7.3324547 5
GGCCC 552560 0.8316191 7.9487987 1
CCCCG 452430 0.8109242 8.251004 1
GTCGT 1084770 0.782436 10.464305 7
GCCCT 628190 0.7794762 5.4450145 1
GCCTA 688645 0.6969907 5.2670307 3
CCCGG 406220 0.6113731 6.8789473 2
GGTAA 823230 0.5706749 5.110843 7
Produced by FastQC (version 0.10.1)