FastQCFastQC Report
Mon 21 Jul 2014
13S15C_GGCTAC_L004_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 13S15C_GGCTAC_L004_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 11820831
Filtered Sequences 0
Sequence length 101
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 255373 2.1603641909777744 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 127304 1.0769462823721954 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 101082 0.8551175463044857 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 82895 0.7012620347926469 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 73582 0.6224773875880638 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 71808 0.6074699824403208 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 65645 0.5553332079614369 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 63000 0.5329574545139847 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 58293 0.49313791898386844 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 56831 0.48076992218228987 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 50951 0.43102722642765134 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 43106 0.3646613338774575 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 39175 0.3314064806442119 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 35741 0.3023560695521322 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 35296 0.2985915288019937 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 34292 0.2900980480983105 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 32925 0.27853371729957055 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 32865 0.278026138771462 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 30946 0.2617920855141233 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 30885 0.26127604734387966 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 30877 0.2612083702067985 No Hit
TGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 30816 0.2606923320365548 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 29644 0.25077763145416765 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 28687 0.24268175393083619 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 26767 0.22643924103136234 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 26228 0.2218794939205205 No Hit
GCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATG 25600 0.2165668386596509 No Hit
TGACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 24413 0.20652524344523665 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 24299 0.20556084424183038 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 24126 0.20409732615245071 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 23573 0.19941914405171685 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 21743 0.18393799894440585 No Hit
GACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCT 21092 0.17843077191442802 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 20754 0.1755714128727498 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 19379 0.1639394049369287 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 19274 0.16305114251273872 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 19015 0.16086009519973682 No Hit
ATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAA 18935 0.1601833238289254 No Hit
ATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTG 18881 0.15972650315362769 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 18850 0.15946425424743826 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 18484 0.1563680252259761 No Hit
GGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCAT 18176 0.15376245544835215 No Hit
TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC 17975 0.15206206737918848 No Hit
GTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGG 16431 0.13900037992252828 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 15946 0.13489745348698412 No Hit
TACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGA 15791 0.133586208956037 No Hit
TTCATGCGTTGGAGAGACCGTTTCTTATTCTGTGCAGAAGCTATTTACAA 15384 0.13014313460703397 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 15295 0.1293902264570063 No Hit
ACCCAGTTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 14947 0.12644627099397665 No Hit
ACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGACTG 14856 0.1256764435596787 No Hit
CAGTAGTTTCACGGATTAAACTTGAAGTTACTAAGGAACCATGCATAGCA 14484 0.12252945668540562 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 14410 0.12190344316740506 No Hit
GCTCATGGTAAGGCTTTATATGGTTTTGATGTTCTTTTATCATCTGCAGA 14310 0.12105747895389081 No Hit
GGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTATTT 14153 0.11972931513867342 No Hit
GGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTA 13631 0.11531338194412898 No Hit
CCACGTAGTTGGTTAGCTACTTCTCACTTTGTATTAGGATTTTTCTTCTT 13600 0.11505113303793954 No Hit
GCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGT 13474 0.11398521812891159 No Hit
TGACCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 13098 0.11080439268609796 No Hit
ATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCA 12979 0.10979769527201598 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 12968 0.10970463920852941 No Hit
CTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGT 12897 0.1091040046169343 No Hit
ACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGACGG 12838 0.10860488573096087 No Hit
GTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAG 12837 0.10859642608882575 No Hit
ACTCTATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCC 12764 0.10797887221296032 No Hit
GCGACTGGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGT 12570 0.10633770163874265 No Hit
CATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGC 12564 0.1062869437859318 No Hit
GTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCC 12442 0.1052548674454444 No Hit
GCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGA 12055 0.10198098593914422 No Hit
CCTACTCTATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGC 11847 0.10022138037503454 No Hit
TGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCT 11841 0.1001706225222237 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 5558825 4.106614 5.4130764 45-49
CTGCT 3834925 3.6694076 8.468897 4
ACTTC 4310890 3.57942 8.918519 60-64
AGCTG 3308080 3.4406145 8.5686865 7
AACTT 5214850 3.4277337 11.161688 7
CTGGT 3591370 3.1348164 16.988317 1
GGAAG 2761505 3.1219559 8.865838 15-19
AGCAC 2232275 3.0325146 8.673807 80-84
TCAAG 3269805 2.9511313 7.2221084 10-14
TGCTA 3894530 2.9499433 5.033895 95-97
CGGTG 2459620 2.9479585 14.848109 9
TCTCT 4169830 2.905735 10.138891 20-24
CTTCA 3492640 2.9000103 9.274947 60-64
GGTGT 3595945 2.863372 12.89376 1
AGGAA 2894620 2.8397436 8.592562 40-44
CTACT 3415825 2.8362293 9.906958 2
GGTGG 2566440 2.8060625 12.704284 7
TTCAA 4182535 2.7491908 9.8255625 9
TGGTG 3436535 2.736438 10.090769 4
CAACT 2761975 2.732584 10.460462 9
GCTGC 2050135 2.693537 9.405851 3
CCGTG 2019470 2.6532483 7.3739724 60-64
CTTCT 3778730 2.633198 6.889964 20-24
ACCCA 1759040 2.619498 46.28761 1
CAAGC 1923905 2.6135983 10.513773 75-79
TATGC 3448520 2.6121092 9.446707 30-34
CTGAA 2888570 2.6070511 7.8224955 80-84
ACAAC 2193795 2.586175 9.486031 85-89
ATGCA 2864905 2.5856924 7.021653 75-79
GAAGA 2631005 2.5811265 5.3514237 95-97
CAACA 2112985 2.4909112 9.157144 85-89
AAGCT 2749570 2.481598 7.175951 15-19
GAAAC 2303875 2.4776142 5.3727646 75-79
TCAAC 2502075 2.47545 8.834591 8
CGTGA 2365485 2.4602556 9.575344 9
ACATC 2483180 2.4567556 8.119078 90-94
GTTCC 2554455 2.4442036 6.931878 70-74
CCAAG 1767795 2.401525 10.292784 75-79
GCAGC 1526865 2.3902824 6.257146 1
TCTGC 2477030 2.37012 6.4667625 85-89
GCTGG 1974670 2.3667254 9.995047 2
CATGG 2256690 2.3471017 11.658563 2
GAAGC 1878585 2.3280857 9.770834 15-19
ATGGT 3342970 2.3099573 5.1543818 1
CTCTG 2399895 2.2963142 9.093573 45-49
GGCTC 1745015 2.29266 6.507244 10-14
TCCTT 3288295 2.2914395 6.6496058 40-44
CGGAA 1836435 2.27585 7.1447 95-97
ACTGC 1985425 2.2636049 7.9144797 4
CAGCA 1660275 2.2554605 5.342768 35-39
GCTAC 1957190 2.2314138 12.782824 1
ACTGA 2450190 2.2113955 5.851664 85-89
AGAGC 1779325 2.205075 12.49456 95-97
CCTTA 2653130 2.2029483 9.89705 95-97
CAAGG 1769650 2.193085 9.5720825 10-14
GAGAA 2198095 2.1564236 5.088447 80-84
GTGGC 1780645 2.1341784 11.847452 8
TGGCT 2435310 2.1257207 9.206025 9
ATCCG 1857020 2.117209 5.51229 65-69
CTTGG 2424975 2.1166995 8.047833 2
AAGGA 2136165 2.0956674 7.977292 10-14
CAGCT 1837855 2.0953586 5.108256 2
ATCGG 1999480 2.0795867 5.6896873 95-97
CTGCA 1821580 2.0768032 5.1175075 95-97
CTCTC 1969950 2.0662432 5.8293877 15-19
TCGGA 1968640 2.0475113 5.436476 95-97
TTCGG 2337585 2.040419 10.772106 7
CATCC 1628060 2.0347202 9.425699 90-94
TAGCT 2664860 2.0185196 5.972911 6
TTCCA 2414275 2.004622 6.8740234 75-79
TCGGT 2295250 2.003466 11.783287 8
AAACT 2545465 1.993611 7.077495 6
TTTCA 3584275 1.9772362 8.407147 8
GCATG 1892485 1.9683052 5.541562 3
CTTTA 3507800 1.9350492 5.0263505 35-39
GGTAT 2793115 1.930013 6.659691 30-34
CTCTA 2323610 1.9293408 6.3279204 95-97
TATTC 3488945 1.9246478 6.4075017 2
GCTTC 2007990 1.921324 9.679061 15-19
TCTGG 2199565 1.9199449 6.8174624 45-49
GATCG 1840555 1.9142947 6.129528 95-97
CACAA 1617395 1.9066808 8.768106 85-89
CCAGC 1105560 1.8972216 6.514366 1
GTGTA 2740990 1.8939955 11.034593 2
GAGCA 1519385 1.8829376 5.5010962 90-94
CTCCT 1785435 1.8727089 5.9515295 70-74
CATGC 1635165 1.8642696 6.1603136 2
GCTCC 1294245 1.8639923 5.001773 70-74
GCTCT 1940855 1.8570867 5.9912887 10-14
TGCTG 2111230 1.8428396 6.125395 6
TTGGT 3169110 1.8378103 22.054127 7
CCCAA 1230720 1.8327434 45.14049 2
GCAGG 1277140 1.8238953 6.825744 90-94
CACCC 969155 1.8231235 8.72653 50-54
CCTGT 1898860 1.8169043 8.061819 1
CTATG 2369065 1.7944676 5.0808635 35-39
TACTG 2367485 1.7932707 5.253386 3
GTAGC 1719025 1.7878959 8.106073 5
TGTAG 2578545 1.7817477 7.04099 3
GTGAA 2154065 1.7735292 5.063957 65-69
AATGA 2469145 1.7641388 5.8396196 75-79
GCACC 1025765 1.7602875 8.14134 50-54
TGGTA 2544795 1.7584268 11.993204 2
CAGTG 1689900 1.7576039 5.7468553 30-34
AAGTT 2924730 1.7537346 6.3414264 60-64
AGCAG 1414960 1.7535262 6.8799067 20-24
CTTTC 2510720 1.7495886 10.420409 7
GCCTT 1813440 1.7351711 6.910285 35-39
TGAAC 1909405 1.7233154 7.496241 80-84
GCACA 1267815 1.7223091 5.8763404 95-97
ACGGT 1653750 1.7200058 7.9532614 4
GCTGA 1652875 1.7190956 7.8693876 80-84
GCAGA 1365695 1.6924733 5.405457 25-29
CACTG 1484175 1.6921244 5.245832 85-89
ACCAG 1245245 1.691648 5.100562 2
CCACA 1131175 1.6845044 7.241142 60-64
CTGAG 1613150 1.677779 5.679153 80-84
ACACA 1422905 1.6774043 8.582735 85-89
GAACA 1553050 1.6701682 8.044529 80-84
GCTTG 1913250 1.6700277 7.7084346 1
TCCAA 1687245 1.6692904 7.4598503 75-79
AGTGC 1599760 1.6638527 5.3476515 25-29
CGCAG 1060705 1.6605165 7.674754 90-94
AACAC 1407205 1.6588962 8.642886 85-89
CCAAT 1668180 1.6504285 30.40794 3
AGATC 1795950 1.6209176 5.268126 95-97
AGCTT 2129320 1.6128706 6.0615788 15-19
CCGCC 743685 1.6121494 7.010962 45-49
GACGG 1127765 1.6105716 10.493336 3
GGTAC 1546210 1.608157 15.310406 3
ATGCC 1406470 1.603532 8.191454 30-34
AACCA 1353310 1.5953616 5.884064 70-74
GAGCC 1015790 1.5902028 10.419289 95-97
CATGA 1751990 1.5812417 6.609011 65-69
ACTAC 1592225 1.5752819 5.1807423 70-74
GTCTG 1799130 1.5704154 6.4882727 90-94
AATCC 1583740 1.5668871 5.0908113 60-64
CACAC 1045870 1.5574715 6.46279 95-97
TTAGG 2249070 1.554084 6.4208827 35-39
AACAT 1972925 1.545197 5.9182773 90-94
TACTT 2795650 1.5421975 8.690011 5
GGTCA 1455320 1.5136257 34.1532 9
CCTTT 2138510 1.4902152 5.398721 35-39
GGAAC 1200300 1.4875032 5.944823 70-74
CTGTA 1957540 1.4827543 5.640974 8
GTCAA 1641365 1.4813983 6.8310237 10-14
TTCTC 2110995 1.4710412 6.0837135 20-24
ATCTC 1765760 1.4661466 6.8146114 45-49
CACGG 932790 1.4602677 7.0678577 45-49
GTGTT 2518005 1.4602258 5.500643 70-74
GCAAC 1074315 1.459442 5.3945174 1
TCATG 1913755 1.4495893 5.694623 60-64
GCTGT 1654975 1.4445859 6.3113112 7
GTATT 2870225 1.4443951 6.570943 4
TGGTC 1644215 1.4351939 28.9539 8
TTCAT 2598015 1.4331738 5.373714 60-64
CTGCG 1087595 1.4289191 8.131283 15-19
AGCCT 1253290 1.4288898 5.858462 2
ACCAA 1207805 1.4238319 6.3068 3
TGCAC 1239235 1.4128656 5.6216264 50-54
GGAAT 1710485 1.4083116 6.2573614 40-44
ACATG 1560340 1.4082698 10.479545 1
AGAGA 1434900 1.4076971 5.1036224 90-94
ACTTT 2494290 1.3759546 8.664332 6
GCCTG 1037860 1.3635757 6.4192195 6
AACGC 996615 1.3538877 6.598201 95-97
ACGCA 988440 1.342782 5.13779 95-97
CTGAC 1168490 1.3322083 8.674916 1
ATGGG 1402955 1.3311182 10.167605 3
CAGGT 1279065 1.3303094 5.593346 95-97
GGTCG 1103825 1.3229809 10.215312 6
GGCTG 1100915 1.3194932 5.2701836 9
ATTCG 1724880 1.3065242 8.428262 6
GCGGC 722720 1.303803 6.9449415 7
TCTGA 1706725 1.2927725 5.535795 20-24
ACGGA 1041455 1.2906504 6.2015595 45-49
TGCCT 1345175 1.2871166 6.76797 30-34
GAATC 1424220 1.2854162 6.5647407 40-44
GAGAG 1128365 1.2756472 6.918346 95-97
GTACT 1683965 1.2755331 11.156513 4
TGTAT 2532805 1.2745938 6.1797805 3
GTATG 1838650 1.270488 5.3955927 7
TGACG 1209750 1.2582175 7.5765285 2
ATCCT 1488955 1.2363098 6.0376782 90-94
TGGGT 1551330 1.2352902 7.777544 4
GATTG 1757820 1.2146351 5.156806 65-69
ACTTG 1594485 1.2077556 12.145836 8
TAGGA 1455150 1.1980841 5.919441 40-44
GGGTC 998320 1.1965287 10.242045 5
CAATT 1809665 1.1894972 20.371431 4
ACACG 858255 1.1659275 5.6301565 95-97
AAGCC 854055 1.1602218 5.5302787 1
ATTGG 1672665 1.1557939 22.10939 6
CGGTA 1090405 1.1340909 7.4519587 5
TTAGC 1482485 1.1229203 5.443456 20-24
TGGCC 847770 1.1138291 11.280774 1
TCACG 975335 1.1119902 5.1927156 45-49
CTTAT 1994045 1.0999986 6.6174154 95-97
GAACC 808500 1.0983361 6.323214 70-74
GCGAC 700270 1.0962614 5.2181935 20-24
CCTGC 752160 1.0832728 6.646683 15-19
CCATG 929310 1.0595167 5.272209 75-79
TGGGG 964615 1.0546788 5.560576 8
TTATG 2030770 1.0219526 5.989051 95-97
CCAAA 863920 1.0184399 8.177747 4
GGGGT 901750 0.9859443 5.364408 8
AATTG 1612855 0.9671045 17.631855 5
CAAAC 801855 0.94527394 8.284069 5
TCGTG 1076060 0.93926567 7.5074596 8
CAGAC 651450 0.8849858 6.068512 25-29
GACCA 633235 0.86024094 6.5655823 2
CCCAG 476670 0.81800056 6.3041897 2
GGCCT 617265 0.8109837 6.4517107 2
GCCCA 467250 0.8018351 5.45512 1
GCCTA 700840 0.7990354 6.123826 3
CTCAG 700250 0.79836273 5.1958985 35-39
GACTC 632895 0.72157055 5.1381207 25-29
CTTGT 1088130 0.6917215 10.081817 9
GGCCC 349670 0.6914925 5.413477 1
GTCGT 779080 0.68003935 7.2725477 7
TGACC 574495 0.65498817 10.849233 1
CCGAA 458105 0.6223293 6.4852166 4
GACCG 353200 0.55292886 5.697846 2
CGAAC 317905 0.43186954 5.8646913 5