FastQCFastQC Report
Mon 21 Jul 2014
13S15B_GGCTAC_L003_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 13S15B_GGCTAC_L003_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 9487936
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 189378 1.9959873253782487 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 88690 0.9347660017942785 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 86536 0.9120634877806933 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 70241 0.7403190746649219 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 69657 0.7341638898070139 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 58927 0.6210729077430539 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 57448 0.6054846912963999 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 53764 0.5666564361311037 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 45674 0.48139026232891957 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 45052 0.4748345688672436 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 38489 0.40566251711647294 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 38295 0.40361781529723645 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 37057 0.3905696665744794 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 35609 0.37530818083089934 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 34911 0.36795147016168744 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 34449 0.36308212871587664 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 31171 0.3285329917908384 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 28629 0.30174107413878004 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 27838 0.29340417136034647 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 26642 0.28079869004175406 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 26609 0.28045087993848183 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 25931 0.2733049632712531 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 25712 0.2709967689495376 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 24988 0.2633660260777476 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 24802 0.26140564185930426 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 24756 0.26092081565474307 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 23793 0.2507710844592544 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 23422 0.24686085572246694 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 23267 0.24522720220709754 No Hit
GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA 21228 0.2237367537049154 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 19968 0.21045673157997694 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 19584 0.20640948674190046 No Hit
GGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCAT 19017 0.20043347678567816 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 18630 0.1963546128473042 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 18246 0.1923073680092277 No Hit
TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC 17843 0.18805986886926723 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 17677 0.18631027865280708 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 17418 0.1835804963271253 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 17261 0.18192576341155758 No Hit
GCGACTGGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGT 17098 0.1802077922953949 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 17043 0.17962810878994123 No Hit
TACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAAC 16295 0.17174441311577143 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 16085 0.16953107609494836 No Hit
GGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTA 16021 0.16885653528860228 No Hit
ACCTCCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCC 14683 0.15475441655592956 No Hit
ACCCAATTTGGGAAGCTGCTTCCGTTGATGAATGGCTTTACAACGGTGGT 14639 0.15429066975156663 No Hit
CCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCCAGAA 14557 0.1534264143434357 No Hit
CCACGTAGTTGGTTAGCTACTTCTCACTTTGTATTAGGATTTTTCTTCTT 14331 0.1510444421210261 No Hit
GCTCATGGTAAGGCTTTATATGGTTTTGATGTTCTTTTATCATCTGCAGA 14020 0.14776659539018813 No Hit
GTATGGGCTTGGATGTTCTTATTTGGACATCTTGTTTGGGCTACTGGGTT 13533 0.14263376144189843 No Hit
CTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGT 12678 0.13362231785711876 No Hit
ATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAA 12340 0.13005989922360353 No Hit
ATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCATCTC 12289 0.1295223745185465 No Hit
GATGAAATTAGCAGTATTATCCGTAAACAGATAGAAGACTATAGTCAAGA 12263 0.1292483423159684 No Hit
ACCAATAGGGGTGCTTCGTGCACCCCCAGAAGCATGTGTCGAGAGCTCCC 12088 0.12740389479861586 No Hit
CATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGC 11448 0.12065848673515506 No Hit
GGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTATTT 11368 0.11981531072722246 No Hit
CAAGGTGAAACAGGTGAAATTAAAGGGCATTATCTAAATGCTACTGCAGG 11274 0.11882457891790163 No Hit
GTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCTTTCAA 11155 0.1175703546061019 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 11001 0.11594724079083164 No Hit
ACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGACTG 10943 0.11533593818508052 No Hit
ATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGATTTCACTAAA 10777 0.11358634796862037 No Hit
TTCATGCGTTGGAGAGACCGTTTCTTATTCTGTGCAGAAGCTATTTACAA 10748 0.11328069666574479 No Hit
TACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGA 10598 0.11169974165087117 No Hit
GACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCT 10539 0.11107789934502088 No Hit
ATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTG 10528 0.11096196264393016 No Hit
CGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTC 10005 0.10544969949207077 No Hit
GTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGG 9931 0.1046697616847331 No Hit
CCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGATT 9807 0.10336283887243758 No Hit
GGTCCTTACGAGTTAATCGTTCTTCACTTCTTACTTGGTGTAGCTTGCTA 9712 0.10236156736301763 No Hit
GTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAG 9670 0.101918899958853 No Hit
GGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCTGGTACTTT 9662 0.10183458235805976 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGCT 3012050 3.5518029 10.981162 5
ACTTC 3237820 3.5308392 7.062293 60-64
AAAAA 3250985 3.2816453 5.8384714 45-49
AACTT 3627780 3.2026727 6.0429587 60-64
GGAAG 2294005 2.9278965 6.7127404 15-19
AGCTG 2340755 2.87164 8.863281 3
CTACT 2632935 2.871212 12.184816 2
CAACT 2198220 2.8488894 8.293667 9
TTCAA 3206090 2.8303971 7.9920645 55-59
TCAAG 2464355 2.7958617 5.850545 10-14
CTTCA 2555010 2.7862349 7.162774 60-64
TCTCT 2948060 2.705093 9.077549 20-24
AGCAC 1621040 2.6998458 5.9260316 35-39
CTTCT 2933950 2.6921456 6.193874 40-44
CTGGT 2585815 2.669268 11.564857 1
TCAAC 2046080 2.651716 7.932519 55-59
GCTGC 1743015 2.6413665 8.942829 3
AGGAA 2172610 2.5643752 6.9189806 40-44
ACCCA 1298700 2.470848 42.200027 1
ACTGC 1755665 2.4604108 11.240718 4
ACAAC 1582765 2.4378114 7.6485276 85-89
CGGTG 1827610 2.4244828 14.393919 9
CAGCA 1430080 2.3818016 6.7902613 35-39
GAAGC 1629250 2.3754234 7.6147194 15-19
ACATC 1809140 2.3446424 6.803736 90-94
CAAGC 1406795 2.3430204 8.174302 75-79
CTGAA 2059465 2.3365057 6.3960056 80-84
TATGC 2447255 2.3362129 7.5906377 30-34
GGTGT 2581395 2.3326912 12.110067 1
GCAGC 1289610 2.3225505 8.783666 1
GGTGG 1996305 2.3183045 10.282972 7
CAACA 1502110 2.313585 7.4873223 85-89
AAGCT 2031155 2.3043876 5.803197 15-19
GCTAC 1637395 2.2946658 15.932548 1
TGGTG 2510000 2.2681744 8.530004 6
CAAGG 1541385 2.2473176 7.1629586 10-14
CTGCA 1579555 2.2136078 5.76807 95-97
CATGG 1801485 2.2100632 14.468127 2
TCTGC 1869695 2.2047403 5.331281 85-89
CCGTG 1439050 2.1807375 6.2458353 60-64
CTCTG 1845865 2.17664 8.227723 45-49
CGTGA 1744335 2.1399512 12.750179 9
CGGAA 1467395 2.139441 5.8649116 95-97
TCCTT 2331345 2.139205 6.6700106 40-44
ATGGT 2545965 2.1276178 6.285973 1
CCAAG 1275360 2.124115 7.9162397 75-79
ATGCA 1841405 2.0891123 5.7558966 1
GTTCC 1749510 2.0630188 5.2764487 70-74
CAGCT 1464685 2.0526278 6.6030717 2
GCTCC 1162935 2.0131445 6.2191634 70-74
TTTCA 2683570 1.9934498 5.9760256 8
GCTGG 1489105 1.9754267 10.284139 4
CTTGG 1887485 1.9484005 7.037377 2
CTCCT 1446415 1.9483705 7.9133077 70-74
GGTAT 2323770 1.9419339 5.673914 30-34
AAGGA 1639545 1.9351877 6.34808 10-14
GCTTC 1631050 1.9233309 8.703966 15-19
CCTTA 1760550 1.9198776 8.019425 95-97
TGCTG 1849080 1.9087563 8.495104 6
TGGCT 1827755 1.8867428 7.5976715 9
CCCAA 987655 1.8790678 43.015995 2
TTCCA 1720150 1.8758212 5.878234 75-79
CCAGC 908350 1.8687531 6.816145 1
AGCTC 1329325 1.8629327 5.4135785 65-69
CTGTT 2305270 1.8517218 6.818536 8
CCAAT 1427800 1.8504264 29.515055 3
CTCTA 1695805 1.849273 5.417532 25-29
GATCG 1495045 1.8341223 5.5254073 95-97
CCTGT 1547640 1.8249738 9.299363 4
GGCTC 1201545 1.8208224 5.6952586 10-14
TAGCT 1902745 1.8164095 6.371921 2
TCTGG 1758170 1.8149121 5.067202 45-49
CATCC 1132795 1.8134655 7.423835 90-94
CACAA 1171505 1.8043795 7.1704392 85-89
CTTTC 1955845 1.7946522 7.2545667 7
GCAGG 1128275 1.7788109 5.7666564 90-94
TATTC 2385860 1.7723004 6.991425 5
TTCGG 1713750 1.7690585 11.156421 7
CACCC 746465 1.754285 6.293518 50-54
AATGA 1901075 1.7460562 6.1432624 75-79
TACTG 1828460 1.7454951 7.7357454 3
TCGGT 1666020 1.7197881 13.254 8
GCCTT 1441385 1.6996782 5.348343 35-39
GCACA 1017820 1.6951816 5.0964456 95-97
GTGGC 1273300 1.6891425 9.081923 8
GCACC 810305 1.6670446 5.7399116 1
TTGGT 2335420 1.6422036 16.793444 7
ACGGT 1337330 1.6406373 8.3207035 4
AGATC 1444585 1.6389118 5.277985 95-97
TGTAG 1937910 1.6194774 6.6896205 6
GAACA 1198355 1.6157615 6.669892 80-84
TGGTA 1928980 1.6120145 8.8371 2
ACCAG 966050 1.6089585 5.193216 30-34
CCGCC 629905 1.6007639 7.683999 45-49
TGAAC 1410715 1.6004856 6.099551 80-84
CCACA 830025 1.5791681 5.986462 1
CTACC 979500 1.5680588 5.7779837 20-24
ATGCC 1115480 1.5632474 8.816703 9
TTAGG 1869275 1.5621201 5.573315 35-39
CTGTA 1635185 1.5609899 6.9806886 5
CCTCC 787885 1.5580245 6.4219937 1
GCTGT 1500895 1.5493339 8.5863285 7
TACTT 2085455 1.549149 6.4244165 5
GCTGA 1260495 1.5463761 5.892665 80-84
CACAC 812730 1.5462635 5.867659 95-97
GCAAC 922850 1.5370089 6.1749377 1
GCACT 1093840 1.5329211 5.34426 35-39
CGCAG 847985 1.5271965 6.580791 90-94
TCCAA 1175115 1.522947 6.1359468 75-79
ACACA 984100 1.5157338 6.952311 85-89
GTGTA 1805945 1.5091963 11.000784 2
CAACC 783335 1.4903378 5.0188656 75-79
GCTTG 1442890 1.489457 6.8501663 1
ATTCC 1354990 1.4776148 5.222632 80-84
GACGG 932905 1.4707953 10.735847 3
GGTAC 1197200 1.4687259 10.175105 3
CATGC 1044410 1.4636493 5.2227883 2
GTCAA 1289485 1.4629476 5.472349 10-14
ATCTC 1338555 1.4596924 6.023797 45-49
AACAC 947100 1.4587455 6.963964 85-89
GTAGC 1186480 1.4555744 7.6256037 1
TTCAT 1952070 1.450066 5.2367864 60-64
CATGA 1278030 1.4499516 5.2476416 65-69
GCGGC 737195 1.4356571 7.8925395 7
ACATG 1262165 1.4319525 13.546885 1
AACCA 920530 1.4178218 5.3745103 75-79
GTCTG 1364655 1.4086971 5.7009397 90-94
GGCTG 1049225 1.3918878 6.412094 9
GCAAG 953200 1.389752 5.19877 1
ACTTT 1866750 1.3866875 6.334352 6
GGTCA 1119435 1.3733237 24.969673 9
AGCCT 979805 1.373111 6.920449 2
TGTTT 2487745 1.3612161 5.0177913 9
GTATT 2074630 1.3490906 6.5990167 4
AGGTA 1347235 1.3380239 5.5069456 6
TTCTC 1457765 1.337622 5.5714655 20-24
ATGGG 1232905 1.3240721 13.166975 3
CAGGT 1079275 1.3240553 5.150144 95-97
TGGTC 1280365 1.3216867 21.210104 8
GCCTG 868815 1.316603 8.072716 6
GAATC 1157210 1.3128788 5.208893 40-44
CAATT 1484935 1.3109288 19.958466 4
ACCGC 631940 1.3000934 5.3388596 45-49
CTGAC 915585 1.2831122 9.423659 1
GGTCG 962955 1.2774432 13.181645 6
TGCCT 1073285 1.2656155 5.2515554 30-34
GTATG 1507850 1.2600838 5.8354135 7
GTACT 1312830 1.2532614 7.840371 4
AAGCC 731055 1.2175738 7.710379 1
TGACG 989595 1.2140359 8.168402 2
TGGGT 1338365 1.2094204 9.567725 4
TGTAT 1817915 1.1821539 6.437557 3
TGGCC 775785 1.1756251 11.71162 1
ACACG 705440 1.174912 5.1346817 95-97
CGGCT 769965 1.1668056 5.9203424 8
GGGTC 873895 1.1592973 13.124403 5
ATTGG 1353385 1.1309999 17.79738 6
ATTCG 1176185 1.1228166 8.873182 6
GCGAC 607840 1.0947024 5.6007514 20-24
GCTCA 768670 1.0772238 5.616485 1
TCGTG 1019370 1.0522685 10.1898 8
CGGTA 856680 1.0509757 7.8078737 5
TGCGG 780360 1.035215 5.6785936 6
AATTG 1316770 1.0176293 15.908882 5
CTTAT 1368760 1.0167629 5.4802732 95-97
TGGGG 873390 1.0142658 6.243922 8
TTGGC 980040 1.0116693 5.5441 3
CACGG 554230 0.99815226 5.3590107 4
GGGGT 836085 0.9709437 6.2292805 8
AACGG 649745 0.94731885 5.1903954 60-64
GGCCA 508840 0.9164062 5.0317345 1
AGTAG 917245 0.9109738 5.1602654 9
CTACA 692590 0.8975954 5.433907 3
GGCCT 580195 0.879228 7.7986116 5
GCCCA 415190 0.85417265 5.522159 2
CCCTA 491610 0.78700703 5.0718117 2
GCCTA 561450 0.78682303 6.6139455 3
GGCCC 340690 0.7579136 7.4202647 1
GTCGT 703405 0.7261063 10.028063 7
CCCCG 284600 0.7232478 5.5546513 1
GCCCT 416125 0.72034955 6.0851336 1
CCTAT 620145 0.6762674 5.0016932 4
GGTAA 574995 0.5710638 5.3981724 7
Produced by FastQC (version 0.10.1)