FastQCFastQC Report
Mon 21 Jul 2014
13S15A_GGCTAC_L002_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 13S15A_GGCTAC_L002_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 12628534
Filtered Sequences 0
Sequence length 101
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[WARN] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 327430 2.592779177693943 No Hit
GACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCT 171702 1.3596352514076455 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 148606 1.176747831537691 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 121238 0.9600322571091783 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 96974 0.7678959410490561 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 93989 0.7442589931657942 No Hit
TGACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTC 93473 0.7401730082050696 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 84691 0.6706320781176975 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 67963 0.538170147065368 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 57412 0.45462125690915506 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 53707 0.4252829346620914 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 49676 0.39336315680030637 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 49332 0.39063916682648997 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 44207 0.3500564673619282 No Hit
GTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATATTGATGGTAT 43271 0.34264468068898574 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 39002 0.30884028185694395 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 38255 0.30292510595449956 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 37188 0.29447598589036544 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 36888 0.2921004132387813 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 36657 0.29027122229706154 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 33661 0.26654717008324164 No Hit
ACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGACTG 31008 0.2455391892677329 No Hit
TACAACATCGTAGCTGCTCACGGTTACTTTGGTAGATTAATTTTCCAATA 30669 0.24285479217144287 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 29603 0.234413590682814 No Hit
GCTAAAAACTATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGA 29409 0.23287738703478963 No Hit
AACAAGTTTATACACTTGGGTAACTTATTATTATTTTACAAACCAAGATT 29169 0.23097692891352234 No Hit
ATACACTTGGGTAACTTATTATTATTTTACAAACCAAGATTTACCATGAC 28052 0.22213188007412418 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 27872 0.22070653648317376 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 27820 0.22029477055689914 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 27803 0.22016015477330939 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 27754 0.21977214457355068 No Hit
ACCCAATTTGGGAAGCTGCTTCCGTTGATGAATGGCTTTACAACGGTGGT 27108 0.21465674479713956 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 26966 0.21353230707538973 No Hit
ACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGACGG 26248 0.20784676986259848 No Hit
ATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTG 26055 0.20631848479007933 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 24450 0.1936091711041044 No Hit
GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA 24073 0.1906238681386137 No Hit
TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC 23875 0.1890559901885682 No Hit
ACTCTATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCC 22753 0.18017134847164365 No Hit
CATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGC 22668 0.17949826955369483 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 22484 0.17804125166072327 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 22469 0.17792247302814404 No Hit
TACCATGACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTC 22345 0.17694056966548927 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 21842 0.17295752618633325 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 20950 0.1658941568356232 No Hit
ACACTTGGGTAACTTATTATTATTTTACAAACCAAGATTTACCATGACTG 20590 0.16304346965372227 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 20487 0.1622278563766784 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 20384 0.16141224309963453 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 19686 0.15588507739694885 No Hit
CTGCTGCTATCATCCCTACTTCTGCAGCTATCGGTTTGCACTTCTACCCA 19122 0.15141900081197074 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 19046 0.15081718907356942 No Hit
AGTATGCTTCAAAAATACCTTTGGTATTACATTGACAATCTAACGAGGAT 18575 0.14708754001058238 No Hit
GCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATG 17675 0.13996082205583008 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 16834 0.1333013000558893 No Hit
GTATGCTTCAAAAATACCTTTGGTATTACATTGACAATCTAACGAGGATT 16745 0.13259654683591937 No Hit
TATACACTTGGGTAACTTATTATTATTTTACAAACCAAGATTTACCATGA 15762 0.12481258711422877 No Hit
GCTGCTATCATCCCTACTTCTGCAGCTATCGGTTTGCACTTCTACCCAAT 15188 0.12026732477419787 No Hit
CCACGTAGTTGGTTAGCTACTTCTCACTTTGTATTAGGATTTTTCTTCTT 15113 0.11967343161130184 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 15012 0.11887365548526851 No Hit
CTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGC 14888 0.11789175212261375 No Hit
ACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGAT 14706 0.11645057138065272 No Hit
ATGCACCCATTCCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCT 14005 0.11089964995145121 No Hit
CCTACTCTATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGC 13472 0.1066790492071368 No Hit
CATCTTTTCAAGAGTTATTAATTGAGTTTTAGGGAGGGATTTATGTCACC 13430 0.10634646903591502 No Hit
CCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCCAGAA 13241 0.10484985826541703 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 13049 0.1033294917684032 No Hit
GCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGT 12880 0.10199125250801083 No Hit
CCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGATT 12875 0.10195165963048443 No Hit
GCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCT 12796 0.10132609216556727 No Hit
TTCATGCGTTGGAGAGACCGTTTCTTATTCTGTGCAGAAGCTATTTACAA 12663 0.10027292162336499 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 5765835 4.1015606 8.899549 70-74
CTGCT 4070725 3.624049 19.56656 3
AGCTG 3630715 3.484757 8.356702 80-84
ACTTC 4226595 3.3209512 8.041169 60-64
CAAGC 2533650 3.2059193 12.592455 30-34
CTTCT 4556320 3.0029233 6.658805 20-24
AACTT 4759980 2.984989 6.718735 60-64
GGAAG 2831655 2.930075 8.892288 15-19
AGCAC 2280495 2.885593 9.042476 65-69
CTGGT 3555725 2.8626394 14.988529 1
GCTAC 2629335 2.7906802 20.838274 5
ACATC 2965765 2.778116 8.806907 90-94
CGGTG 2545790 2.7685568 8.959158 9
CTACT 3519185 2.7651198 16.46756 6
CTGAA 3210505 2.7195888 7.947173 80-84
ACCCA 1939240 2.7134464 54.0673 1
ACAAC 2412700 2.6943798 10.624154 85-89
GGTGT 3689480 2.686086 9.470842 9
GCTGC 2214615 2.6632557 9.752042 3
TGCTA 3746130 2.6617723 14.887607 4
TCTCT 4034965 2.659315 9.042434 20-24
TGGTG 3637440 2.6481988 8.65492 8
CAACT 2820440 2.6419861 11.89943 9
CTTCA 3351215 2.6331408 7.9077263 60-64
AGGAA 2836015 2.5899801 8.208338 10-14
TCAAG 3051010 2.5844822 7.1059847 10-14
GGTTC 3153865 2.5391107 5.9389195 90-94
ACTGC 2382990 2.5292184 22.170464 2
CAGCA 1987755 2.5151782 9.900608 60-64
GGTGG 2554995 2.5126793 5.1308365 10-14
TTCAA 3987085 2.5003057 10.683409 9
AAGCT 2928840 2.480993 7.246646 75-79
CCAAG 1950440 2.4679623 9.967141 75-79
CAACA 2206990 2.4646533 10.068957 85-89
CTTTA 4644625 2.4431293 10.012712 9
TATGC 3392910 2.4107957 8.138143 8
GCAGC 1649750 2.3652365 7.205176 1
TCAAC 2486360 2.3290439 9.524995 8
GCTTC 2605485 2.3195882 9.338467 15-19
TCTGC 2604230 2.318471 6.5456667 45-49
GAAAG 2525390 2.306303 7.6387115 10-14
CCAGC 1453170 2.3038628 10.531441 60-64
GAAGC 2010640 2.3006814 9.731375 15-19
TGAAA 3324490 2.2476087 6.7024794 70-74
ATGGT 3480340 2.2362785 5.2724013 90-94
GCTGG 2035970 2.2141252 7.046367 2
CTTGG 2749720 2.213742 9.892835 6
CCGCC 1110370 2.2056785 12.356563 75-79
ATCGG 2232290 2.14255 8.370062 85-89
GATGG 2452815 2.1289299 6.5389037 90-94
CCGTG 1769225 2.1276376 5.1762524 60-64
ATGCA 2503890 2.1210215 6.0734 1
CAGCT 1994360 2.116741 6.5532427 2
TCCTT 3208765 2.1147933 5.658312 90-94
CTCTG 2370405 2.1103032 9.433816 20-24
GTTCC 2368880 2.1089456 6.754418 70-74
CAAGG 1837680 2.1027713 9.30203 10-14
TTCCA 2659185 2.089394 7.0615325 75-79
TCGGA 2171025 2.0837476 8.722188 85-89
CGTGA 2157120 2.070402 10.130552 9
CTCCT 2061690 2.0296888 6.1312156 70-74
CATGG 2113505 2.0285401 13.044785 2
ACTGA 2349385 1.990142 5.726259 65-69
TTCGG 2471775 1.9899743 8.0026455 95-97
CACAA 1773560 1.9806209 8.76194 85-89
CGGAA 1729445 1.9789231 5.253216 95-97
CCTTA 2515090 1.9761747 10.072065 95-97
TCGGT 2454500 1.9760664 8.599792 8
GCTCC 1485805 1.9758782 5.064292 65-69
GCCTT 2219125 1.9756231 6.799205 35-39
ACCAG 1540510 1.949263 8.653705 60-64
CTCTC 1972590 1.9419718 5.6732163 95-97
GAAAA 2408775 1.9414834 6.302702 70-74
AAAGA 2363870 1.9052894 5.9473457 15-19
GGCTC 1573365 1.8921001 6.434064 90-94
CATCC 1608430 1.8877742 9.13124 90-94
TTTCA 3570435 1.878092 9.148727 8
TTGGT 3479860 1.8755308 23.261452 7
AAGGA 2053000 1.8748946 7.990177 10-14
GGTAT 2915440 1.8733047 7.053683 6
CACCC 1067445 1.8714123 9.252401 50-54
AGAAA 2318640 1.8688341 11.913304 9
TGCTT 3114860 1.8564565 7.0291457 4
CCCAA 1322350 1.8502743 53.796535 2
CCACA 1322075 1.8498895 9.346979 1
GCAAG 1614325 1.8471966 8.779459 25-29
AGCCT 1740200 1.8469847 7.2220526 30-34
CTATG 2577010 1.8310666 6.9721694 35-39
AAACC 1638815 1.8301444 7.8144393 70-74
GCACC 1144280 1.8141471 8.488188 50-54
CCTGT 2028540 1.8059506 6.1261168 1
TACTT 3429140 1.8037692 11.914609 3
GCACT 1696445 1.8005447 6.877473 65-69
GTGTA 2797460 1.797497 7.35785 2
TCTGG 2231500 1.796534 6.4571733 45-49
CACTG 1687360 1.7909021 7.4341908 65-69
CTTTC 2706450 1.783734 11.278176 7
ACCGC 1123780 1.7816463 9.673541 75-79
GGTCG 1631795 1.7745833 11.21315 6
GCTTG 2195890 1.7678651 6.86279 5
TGGTA 2737985 1.7592815 11.731802 2
GACTG 1823755 1.7504385 18.465328 1
CATGC 1642060 1.7428224 8.943024 2
ACACA 1555855 1.7374991 8.56521 85-89
TCCAA 1846880 1.7300248 7.4160256 75-79
TGAAC 2041030 1.7289375 7.518843 80-84
AAGCC 1361565 1.7228376 8.184126 30-34
CCTTT 2607700 1.7186508 5.370623 75-79
GCTGA 1781500 1.7098821 7.666842 80-84
CCAAT 1821250 1.7060164 36.122913 3
AAAAC 1910125 1.7024837 6.684956 70-74
GAACA 1681135 1.6977521 8.062123 80-84
ATGGG 1942105 1.6856571 10.620823 3
ACTTT 3200020 1.6832494 12.232192 4
ACGGT 1748180 1.6779016 10.566181 4
AACAC 1500650 1.675849 8.651334 85-89
TTACA 2663135 1.670055 5.3582296 80-84
TTTAG 3505475 1.6674731 8.186734 6
CATGA 1963215 1.663021 6.4644446 65-69
AAAGC 1639110 1.6553116 7.6773305 20-24
GGATG 1905765 1.6541157 6.704075 85-89
GGGTC 1512385 1.6447246 11.174651 5
CTGCG 1363080 1.639215 8.712902 45-49
CTCTA 2072335 1.62829 6.837937 95-97
ATGCC 1517330 1.6104383 11.035271 9
GCGAC 1119120 1.6044755 8.902392 50-54
AGCTT 2253040 1.6008732 5.972326 15-19
AACAT 2111015 1.5782316 6.131734 90-94
ACATG 1858380 1.5742164 13.896402 1
GGTAC 1619835 1.5547162 15.836938 3
TACCA 1659605 1.554599 5.974051 60-64
CTGGG 1421425 1.5458051 6.901193 55-59
GACGG 1184415 1.5355943 13.938354 3
TGGGT 2104940 1.5324787 8.173346 8
GACGC 1063525 1.5247692 9.365974 15-19
TGCAC 1434130 1.5221331 5.9039207 50-54
GCGCA 1060750 1.5207907 9.391388 25-29
TTAGG 2354155 1.5126531 6.6926174 35-39
CTGTA 2120010 1.5063503 6.766739 8
CATCG 1417050 1.504005 6.6730523 85-89
ACTGG 1543815 1.4817523 7.0173006 50-54
TTCAT 2811625 1.4789488 5.108343 60-64
AGCGC 1027570 1.4732208 8.907344 25-29
CGCCT 1103375 1.4673089 8.635917 75-79
TGGGG 1482905 1.4583452 6.578277 35-39
GTGTT 2694420 1.4522043 5.360938 70-74
ATCTC 1820875 1.4307113 6.830187 45-49
GCGAA 1241120 1.4201555 7.270609 20-24
AACCG 1121905 1.4195869 8.838097 70-74
CGCAA 1121060 1.4185177 8.259356 25-29
GCAAC 1115225 1.4111346 9.650481 1
ATGCT 1979060 1.4061996 7.0685625 3
TCATG 1958650 1.3916976 5.5591235 60-64
GGGGT 1410645 1.3872819 7.0053062 35-39
CGCGA 966870 1.3861955 8.6952 20-24
TCGCT 1551685 1.3814204 5.9819913 40-44
GTCAA 1607800 1.3619523 6.6994634 10-14
TGGTC 1683290 1.3551815 28.365612 8
AGACG 1181890 1.3523815 7.685883 15-19
GGAAT 1762200 1.349899 6.1411176 40-44
AAGAC 1332175 1.3453429 6.661389 15-19
AAGCG 1175615 1.3452013 7.1832237 25-29
GGTCA 1401470 1.3451296 33.525078 9
TTCTC 2030030 1.3379273 5.7863193 20-24
GCGGC 821355 1.3342519 6.772484 7
GTCGC 1100755 1.3237478 7.742108 40-44
GTATG 2034670 1.3073692 7.1225686 7
ACAAG 1286275 1.2989892 5.261296 2
GGCTG 1188130 1.292096 5.203674 9
CACAT 1377565 1.2904042 6.3106475 2
ACGCG 894430 1.2823387 9.909815 15-19
TCTGA 1803080 1.281159 5.7573934 20-24
GAATC 1511115 1.2800514 6.4725738 40-44
GTACT 1798500 1.2779047 11.84503 4
CTGAC 1201930 1.2756845 11.736223 1
CAATT 2032585 1.2746364 24.622854 4
CGACT 1194030 1.2672998 6.909766 50-54
GTTAC 1783015 1.2669021 5.375959 55-59
TGCCT 1422390 1.2663128 6.6899242 30-34
CGGAT 1304400 1.251962 6.1023674 85-89
CGAAA 1238385 1.2506258 6.727129 20-24
ATCCT 1588325 1.2479904 6.0237975 90-94
GCCTA 1171920 1.2438331 6.7982373 30-34
GGGTT 1708070 1.2435418 5.1442885 55-59
TGCGA 1284005 1.2323868 6.086575 50-54
GATTG 1914275 1.2300099 5.060511 65-69
TGTAT 2552210 1.2140272 5.17872 9
CTTAT 2292225 1.2057382 6.612373 95-97
TGACG 1250620 1.200344 10.266497 2
CACGG 831725 1.192439 5.810313 4
TAGAA 1752995 1.1851581 9.818781 8
ATTCG 1615480 1.1478618 5.3212357 6
TAGGA 1489950 1.1413472 5.8120346 40-44
ATTGG 1753440 1.1266661 23.146872 6
CGCTT 1259665 1.1214435 6.4387107 40-44
GTTCG 1382515 1.1130339 5.6569395 90-94
TGGCC 904760 1.0880479 7.613331 1
CGGTA 1132360 1.0868381 10.069081 5
TTATG 2227795 1.0597105 6.06568 95-97
GCTCA 989400 1.050113 5.465459 1
AATTG 1778565 1.0086106 20.188118 5
TTAGA 1773560 1.0057724 8.802508 7
TCGTG 1243085 1.0007817 8.472515 8
CCTAT 1266590 0.9951944 5.577702 30-34
CTACA 960580 0.8998025 6.4314413 3
TGACT 1029570 0.73154986 7.770288 1
GTCGT 889380 0.7160212 8.2530985 7
Produced by FastQC (version 0.10.1)