FastQCFastQC Report
Mon 21 Jul 2014
13L15B_CAGATC_L003_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 13L15B_CAGATC_L003_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 13719692
Filtered Sequences 0
Sequence length 101
%GC 46

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[OK] Per base sequence content

Per base sequence content

[WARN] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GCGAGTCGGGCTGTTTGGGAATGCAGCCCTAAGTGGGAGGTAAATTCCTT 220296 1.605692022823836 No Hit
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 193179 1.4080418131835613 No Hit
GATACCGTCCTAGTCTCAACCATAAACGATGCCGACTAGGGATTGGCGGA 134561 0.9807873237970648 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 80264 0.5850277105346097 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 75729 0.5519730326307617 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 70698 0.5153031132185767 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 66271 0.4830356249979956 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 63463 0.4625686932330551 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 62975 0.4590117620716267 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 59883 0.43647481299142865 No Hit
GACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCT 56979 0.4153081570635842 No Hit
CATGGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAAC 53872 0.39266187608293246 No Hit
CAGACCTCTGATCAGGAAAGACTACCCGCTGAGTTTAAGCATATCACTAA 47398 0.3454742278470974 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 45512 0.3317275635633803 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 43551 0.31743423977739443 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 43134 0.3143948129447804 No Hit
CAACACGGGGAAACTTACCAGGTCCAGACATAGTAAGGATTGACAGATTG 39658 0.2890589672129666 No Hit
CCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCCAGAA 37242 0.27144924244655055 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 36919 0.2690949621901133 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 35736 0.2604723196409948 No Hit
CAAACGAATAAGGGCTTATGGTGGATACCTAGGCACCCAGAGACGAGGAA 34668 0.25268788832868844 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 34478 0.2513030175896077 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 34346 0.2503408968656148 No Hit
TGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGT 34270 0.2497869485699825 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 33714 0.24573437945983045 No Hit
TGGGTCGGGGGGAGCGGTCCGCCCCTTGTGGGTGTGCACTGGTCCACTCG 33704 0.24566149152619463 No Hit
AGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCGGGG 33287 0.24262206469358058 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 33020 0.24067595686550397 No Hit
GATCAGGAAAGACTACCCGCTGAGTTTAAGCATATCACTAAGCGGAGGAG 32672 0.23813945677497714 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 31727 0.23125154704639142 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 29668 0.21624392151077446 No Hit
ACCTCCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCC 29081 0.21196539980635135 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 29006 0.21141874030408264 No Hit
TGACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTC 28976 0.21120007650317515 No Hit
ACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGACTG 28520 0.2078763867293814 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 27599 0.2011634080415216 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 27298 0.19896948123908323 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 24198 0.17637422181197654 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 23828 0.1736773682674509 No Hit
CTGATCAGGAAAGACTACCCGCTGAGTTTAAGCATATCACTAAGCGGAGG 22666 0.16520779037896768 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 22203 0.16183307905162886 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 21162 0.15424544516013916 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 21123 0.15396118221895944 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 21052 0.15344367789014507 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 20905 0.15237222526569838 No Hit
GGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTATTT 20573 0.1499523458689889 No Hit
AAGTGGGAGGTAAATTCCTTCTAAGGCTAAATATTGGCAAGAGACCGATA 20378 0.14853103116309027 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 19883 0.14492307844811678 No Hit
GAAACATCTTAGTAGCCAGAGGAAAAGAAAGCAAAAGCGATTCCCATAGT 19881 0.1449085008613896 No Hit
GTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATATTGATGGTAT 19551 0.14250319905140726 No Hit
CCACGAGGCGCGATCTGCGAGTCGGGCTGTTTGGGAATGCAGCCCTAAGT 19312 0.140761177437511 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 18987 0.13839231959434659 No Hit
GACACTGAGAGACGAAAGCTAGGGGAGCGAATGGGATTAGATACCCCAGT 18941 0.13805703509962178 No Hit
ATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTG 18153 0.13231346592911852 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 17859 0.1301705606802252 No Hit
TGGCAAGAGACCGATAGCGAACAAGTACCGCGAGGGAAAGATGAAAAGGA 17858 0.13016327188686158 No Hit
CAACGCGAAGAACCTTACCAGGGCTTGACATGCCGCGAATCCTTTTGAAA 17728 0.12921572874959583 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 17436 0.12708740108742966 No Hit
ACCAATAGGGGTGCTTCGTGCACCCCCAGAAGCATGTGTCGAGAGCTCCC 17277 0.12592848294262 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 17212 0.12545471137398712 No Hit
ACATCGGTAGGGGAGCGTTCCGCCTTAGAGGGAAGCATCAGCGCGAGCAG 17142 0.12494449583853633 No Hit
AACACGGACCAAGGAGTCTAACATGTATGCAAGCAGGTGGGCGGCAAACC 17052 0.12428850443581387 No Hit
TACCATGACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTC 17037 0.12417917253536014 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 16856 0.12285990093655164 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 16803 0.12247359488828174 No Hit
TTCATGCGTTGGAGAGACCGTTTCTTATTCTGTGCAGAAGCTATTTACAA 16772 0.1222476422940107 No Hit
CCACGTAGTTGGTTAGCTACTTCTCACTTTGTATTAGGATTTTTCTTCTT 16464 0.12000269393802718 No Hit
GCGACTGGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGT 16070 0.11713090935277556 No Hit
GCAACACGGGGAAACTTACCAGGTCCAGACATAGTAAGGATTGACAGATT 15938 0.11616878862878263 No Hit
GTAGGTGGCTTTTTAAGTCTGCTGTCAAATCCCAGGGCTCAACCCTGGAC 15821 0.11531599980524344 No Hit
ATCACTAAGCGGAGGAGAAGAAACTAACCAGGATTTCCCTAGTAACGGCG 15691 0.11436845666797768 No Hit
GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA 15504 0.11300545230898769 No Hit
GCTAAAAACTATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGA 15130 0.11027944359100773 No Hit
GTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCATCTCTGCT 14756 0.10755343487302776 No Hit
ACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCA 14617 0.10654029259548976 No Hit
AAGCGGAGGAGAAGAAACTAACCAGGATTTCCCTAGTAACGGCGAGCGAA 14568 0.10618314172067418 No Hit
GTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAG 14524 0.10586243481267654 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 14472 0.10548341755777024 No Hit
TAGTAGCCAGAGGAAAAGAAAGCAAAAGCGATTCCCATAGTAGCGGCGAG 14472 0.10548341755777024 No Hit
TACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGA 14385 0.10484929253513854 No Hit
TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC 14124 0.10294691746724344 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 13789 0.10050517169044319 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGCT 3669970 3.206544 8.738556 5
CTTCT 4033930 3.1798744 8.104845 45-49
ACTTC 3456615 2.9231825 5.922901 60-64
AGCAC 2611260 2.6258585 6.7488565 70-74
CTACT 3034615 2.566306 12.629648 2
TGAAA 4117810 2.5130553 5.228358 65-69
TCTCT 3155020 2.4870455 8.136685 20-24
CTGGT 3244125 2.3629847 8.448 1
CTGAA 3107200 2.3500981 5.822589 80-84
TGCTA 3222325 2.2717624 5.690066 4
AGCTG 2889915 2.2582486 5.463282 3
TCCTT 2863420 2.2571824 8.276565 45-49
TTCAA 3232930 2.206074 6.2599564 55-59
CAACT 2423190 2.198445 5.7129984 55-59
TCAAC 2414850 2.1908784 5.661165 55-59
GCAGC 2098475 2.1801925 6.6472063 20-24
ATCGG 2775450 2.1688027 6.055799 85-89
GGTGG 3187685 2.1454606 6.272637 7
CTTCA 2536440 2.1450107 5.676578 60-64
TGGTG 3522690 2.1390774 5.9592214 6
CGGTG 2621695 2.116599 8.391863 9
CAAGC 2098720 2.110453 5.6743617 2
GAAAG 3090145 2.0902953 6.005084 7
ACTGC 2227720 2.088135 7.358852 4
AGATC 2759575 2.0871758 8.145546 95-97
GGTTC 2843650 2.0712833 5.6258664 3
TCGGA 2644970 2.0668423 5.40787 85-89
CTCTG 2326655 2.0328562 6.5030885 20-24
CTTTA 3193235 2.0311003 6.1385736 5
CTCCT 1924405 2.016891 8.441795 70-74
AGGAA 2951365 1.9964193 5.6347647 10-14
TATGC 2827180 1.9931825 5.728076 30-34
GCTGC 2030980 1.9668597 5.6130695 3
ATGGT 3340450 1.9633025 6.201522 1
TTTCA 3075005 1.9558985 5.6574697 8
GCTTC 2238155 1.9555317 6.747974 15-19
ACCCA 1618090 1.9517994 27.049425 1
TTCCT 2472000 1.9486331 7.041728 45-49
GGTGT 3195995 1.9406989 7.0852246 1
CCGCC 1251740 1.9332923 5.940616 65-69
CTGTT 2932150 1.926888 5.1733017 8
CATGG 2400205 1.8755774 9.111786 2
CCAAG 1863880 1.8743001 5.1472816 75-79
GCTCC 1607090 1.8668922 6.305653 70-74
ACAAC 1907690 1.8567748 5.461738 85-89
CTTTC 2350130 1.8525652 6.6114845 7
GCACC 1476810 1.8404626 7.0830784 3
CCTTA 2164575 1.8305328 6.40244 95-97
GCTAC 1939655 1.8181195 13.775543 1
AAAGA 2765395 1.8105735 5.311629 8
GATCG 2288070 1.7879525 6.068225 95-97
TGCAG 2285540 1.7859753 5.3135223 20-24
CAGCT 1879725 1.7619447 5.103214 65-69
ACTTT 2749910 1.7491176 7.2693725 4
CAACA 1771300 1.7240249 5.940723 1
CTACC 1501865 1.6886519 6.442397 20-24
TACTT 2640370 1.6794432 6.862828 3
TGCTG 2297085 1.6731714 5.538609 6
CCTGT 1911945 1.6705139 6.4710126 1
TGTTT 3367060 1.6642431 5.151111 10-14
CCAGA 1650110 1.6593351 6.2757096 7
TTCGG 2277270 1.6587381 7.201696 7
CTTGG 2271890 1.6548195 5.3584723 2
CACAC 1355725 1.6353251 5.676222 95-97
GCTGG 2023315 1.633503 6.436508 4
GTCTG 2241360 1.632582 5.1727505 90-94
CGTGA 2088575 1.6320622 7.378594 9
ACCGC 1295640 1.6146808 5.6218295 90-94
GGAAA 2375630 1.6069692 5.309336 9
ATGCC 1710485 1.6033089 6.999858 9
TGGGA 2461165 1.6033047 5.6456795 15-19
TATTC 2518725 1.6020694 5.36466 5
CACCC 1059320 1.583583 7.8975625 4
AGCCT 1659480 1.5554999 6.108361 2
TTCAT 2440975 1.5526152 5.308236 60-64
TTGGT 2808185 1.5384548 13.617866 7
AGAAA 2333540 1.5278271 5.1036596 9
GCTTG 2092955 1.5244853 5.2127843 1
GCTGT 2050650 1.4936708 5.581774 7
GGGAA 2134140 1.4914939 5.7402897 8
CAGCC 1191325 1.484679 6.467422 20-24
CCCTA 1313930 1.4773434 5.8787293 25-29
TCGGT 1995860 1.4537623 8.285802 8
CCCAA 1204065 1.4523872 28.136475 2
GGAAT 2295230 1.4472106 5.1321354 15-19
GAACA 1778905 1.4434205 7.817518 80-84
TACTG 2041435 1.4392264 5.316381 3
GCGAG 1658785 1.4367123 20.422844 1
TGGTA 2441050 1.434693 6.4326024 2
CCAAT 1551565 1.4076611 20.436472 3
ACACG 1370990 1.3786547 6.13032 3
CTGTA 1953180 1.3770059 6.1958146 8
CCTCC 983785 1.3708519 5.3005385 2
CATCC 1209365 1.3597739 5.436613 90-94
ACGGT 1717250 1.3419001 6.6808395 4
ATGGG 2030525 1.3227679 7.578751 3
TGGAG 2006320 1.3069999 5.039759 3
AAGCC 1270535 1.277638 5.465707 1
ATTGG 2165970 1.2730186 12.714407 6
AACAC 1307500 1.2726035 8.560694 2
GGCTG 1576045 1.2724041 22.046553 9
TGGGT 2092825 1.2708228 6.3070035 4
CCCAG 1013110 1.26258 5.793367 6
AGCCC 997595 1.2432448 7.0786304 25-29
CGAGG 1434160 1.2421594 5.01927 95-97
GACGG 1427215 1.2361442 6.94002 3
GTATT 2327610 1.2342395 5.023879 4
ACATG 1623595 1.2279892 8.926463 1
GTGTA 2088250 1.2273396 6.753819 2
CAGGA 1461655 1.2253348 5.0443335 4
TGGTC 1655020 1.2054983 15.222606 8
AGGTA 1867240 1.1773504 5.775072 35-39
GAGTC 1506190 1.1769727 17.91473 3
GCCCT 989145 1.1490502 6.313587 25-29
AAGAC 1403805 1.1390607 6.263648 9
GCAAC 1124160 1.1304448 7.3636374 1
CCAGT 1202895 1.1275237 5.0151467 3
TCCTA 1322545 1.1184468 12.150009 8
GGTCG 1378410 1.1128455 7.3914 6
TACCG 1160610 1.0878882 14.188598 3
CCTTC 1031595 1.0811731 5.567869 45-49
GCCAG 1038560 1.079003 6.4399424 2
CAATT 1580255 1.0783283 15.455365 4
GACTG 1350720 1.0554848 5.7589455 1
GGGGA 1460130 1.0542896 5.3035307 8
AGACC 1044315 1.0501533 5.925504 2
CTGAC 1118555 1.0484682 8.651199 1
ACCGT 1111980 1.0423052 13.56767 4
GTACT 1471635 1.0375133 6.0049453 4
GGTAC 1326145 1.0362813 6.627029 3
GGGCT 1277775 1.0315988 19.222118 8
GGGTC 1263230 1.0198561 7.222671 5
GTCCT 1157425 1.011271 12.658843 7
GTTCG 1379945 1.0051367 5.3521147 4
GCCTA 1065595 0.9988267 5.216971 3
TGACG 1265970 0.98925906 5.966903 2
CCTAG 1044875 0.9794049 13.075129 9
CCGCG 757710 0.9756075 5.495993 90-94
GGTCA 1223215 0.95584935 16.10361 9
TGGCC 984810 0.9537185 5.412823 1
CGGTA 1206350 0.9426706 7.4762497 5
TCGGG 1165000 0.940551 19.086325 6
CCCCG 608570 0.93992656 7.322071 1
AATTG 1620910 0.9220859 12.039797 5
TCGTG 1234440 0.8991524 6.580743 8
CGGGG 1003650 0.8981147 8.536686 6
CGTCC 765850 0.8896574 16.20841 6
ATTCG 1257615 0.8866277 5.7806563 6
ACCTC 766780 0.8621444 6.4176755 4
AGGCA 996405 0.8353064 6.1937327 1
TGCCA 883810 0.828432 5.846833 1
CGAGT 1039255 0.8120987 17.971952 2
GTCGG 944015 0.762141 19.059347 5
CACGG 714815 0.7426508 8.936414 4
CCGTC 630520 0.7324498 16.366173 5
CGGGC 681715 0.73175156 24.927301 7
CAGAC 725900 0.7299582 6.3409233 1
AGTCG 914845 0.7148817 18.227364 4
CCTCT 671835 0.70412314 6.098501 5
ATACC 722895 0.6558483 13.014668 2
ACGGG 754405 0.6534077 5.5119452 5
GGCAC 602780 0.62625307 5.5234766 2
GTCGT 855340 0.62302023 6.554929 7
CCACG 491120 0.6120543 5.460259 1
CCCGG 456580 0.58788043 6.2896 2
GACCT 623735 0.5846529 5.373339 3
GATAC 759790 0.5746593 10.784857 1
Produced by FastQC (version 0.10.1)