FastQCFastQC Report
Mon 21 Jul 2014
13L15A_CAGATC_L002_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 13L15A_CAGATC_L002_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 13304063
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[OK] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 223468 1.6796973977047465 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 138051 1.0376604500444715 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 104493 0.7854217166590387 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 89504 0.6727568863737341 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 64508 0.48487443272029007 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 61673 0.46356515299123285 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 61001 0.45851406446286375 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 52731 0.39635260296046404 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 51894 0.39006129180236143 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 50954 0.3829957810632737 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 48704 0.36608365429418066 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 41926 0.31513681196488624 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 37935 0.28513845732690835 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 37652 0.28301128760439576 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 37229 0.2798318077718063 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 35910 0.2699175432347246 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 35414 0.2661893588447379 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 35124 0.26400957361672145 No Hit
GACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCT 34100 0.25631267681158754 No Hit
TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC 33082 0.24866087901117126 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 31959 0.24021984862819726 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 31939 0.24006951861247197 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 31550 0.23714559980661545 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 28323 0.21288985176934294 No Hit
GCTCATGGTAAGGCTTTATATGGTTTTGATGTTCTTTTATCATCTGCAGA 27239 0.20474196491703325 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 27230 0.20467431640995687 No Hit
ACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGACTG 26992 0.20288538922282615 No Hit
CCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCCAGAA 25598 0.19240738712677474 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 24894 0.1871157705732452 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 24742 0.1859732624537331 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 24188 0.18180912101814312 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 22986 0.1727742870730543 No Hit
ACCCAATTTGGGAAGCTGCTTCCGTTGATGAATGGCTTTACAACGGTGGT 22949 0.17249617654396254 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 22867 0.17187982347948894 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 22606 0.16991801677427415 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 22185 0.16675356994325718 No Hit
ACCTCCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCC 22028 0.1655734793198138 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 21231 0.15958282819316175 No Hit
GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA 21001 0.15785403301232112 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 20810 0.15641838136214478 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 20127 0.15128461132512677 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 19981 0.1501872022103323 No Hit
CCACGTAGTTGGTTAGCTACTTCTCACTTTGTATTAGGATTTTTCTTCTT 19860 0.1492777056151944 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 19742 0.1483907585224153 No Hit
GCTAAAAACTATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGA 19162 0.14403118806638243 No Hit
TTCATGCGTTGGAGAGACCGTTTCTTATTCTGTGCAGAAGCTATTTACAA 18325 0.13773987690827982 No Hit
TGACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTC 18119 0.13619147774630952 No Hit
GTATGGGCTTGGATGTTCTTATTTGGACATCTTGTTTGGGCTACTGGGTT 18035 0.13556009168026337 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 17618 0.1324257108523915 No Hit
ACCAATAGGGGTGCTTCGTGCACCCCCAGAAGCATGTGTCGAGAGCTCCC 16968 0.12753998534132016 No Hit
CCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGATT 16601 0.12478142955276145 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 16502 0.12403729597492134 No Hit
GCGACTGGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGT 16322 0.1226843258333939 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 16203 0.12178986223982854 No Hit
CAAGGTGAAACAGGTGAAATTAAAGGGCATTATCTAAATGCTACTGCAGG 15301 0.11500997853061881 No Hit
TACAACATCGTAGCTGCTCACGGTTACTTTGGTAGATTAATTTTCCAATA 14705 0.1105301440620057 No Hit
GTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATATTGATGGTAT 14569 0.10950789995507389 No Hit
TACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAAC 14402 0.10825264432376787 No Hit
CATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGC 14384 0.10811734730961511 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 5397480 3.7301462 5.350123 90-94
CTGCT 3736890 3.2202458 9.222187 4
ACTTC 3792185 3.0481372 6.2546754 60-64
AACTT 4378495 2.8228505 5.5063105 60-64
AGCTG 3229210 2.7663276 6.737055 3
GCTGC 2421945 2.5867448 9.1872635 3
GGAAG 3026145 2.5770662 5.77367 15-19
CTACT 3175790 2.5526826 8.895986 2
TTCAA 3952875 2.5484498 6.9218373 55-59
CTTCT 3655140 2.5413947 5.5329013 20-24
CTTCA 3155190 2.5361242 6.2527266 60-64
CAACT 2637635 2.4509623 7.7989583 9
TGCTA 3437600 2.3760304 5.541812 1
TCTCT 3414845 2.3743193 7.677924 20-24
CTGGT 3181065 2.3572352 10.050198 1
TCAAC 2508670 2.3311245 7.1302323 8
CAAGC 2021460 2.328079 7.3739552 2
CAGCA 2010600 2.3155715 7.088 2
TGGTG 3520695 2.2434194 7.026606 6
GGTGG 2828930 2.234163 7.146945 7
CTGAA 2768270 2.2119844 5.588517 80-84
GCAGC 1786840 2.2062356 7.2925153 1
ACTGC 2206020 2.197683 5.5418177 2
AGGAA 2742615 2.1785476 5.717714 10-14
CGGTG 2362285 2.1695678 11.554576 9
ACAAC 2018725 2.1685827 6.8712153 85-89
GGTGT 3392925 2.162003 10.053981 1
GAAGC 2175690 2.1546726 6.422101 15-19
ACCCA 1592510 2.1328635 36.405907 1
CAACA 1975360 2.1219988 6.675306 85-89
AAGCT 2628990 2.1006927 5.1062245 15-19
GCTAC 2095035 2.0871174 11.260213 1
CATGG 2416500 2.0701132 15.875833 2
CAGCT 2072370 2.0645382 6.018653 2
TCCTT 2961260 2.0589447 6.837291 40-44
ACATC 2213160 2.0565283 5.6513877 90-94
CAAGG 2053085 2.0332515 6.0451355 10-14
TATGC 2932835 2.0271425 6.659062 30-34
CCAAG 1753085 2.0189962 6.7246556 75-79
GCTCC 1603470 1.9915833 6.5246897 65-69
GCTGG 2142620 1.9678235 7.86412 4
CTTGG 2647450 1.9618155 6.5316668 2
CTCCT 1944705 1.9488605 7.734534 70-74
CCAGC 1342955 1.9283062 7.869595 1
GCTTC 2216935 1.9104322 7.703535 15-19
AGCTC 1872495 1.8654187 5.5969954 65-69
TTTCA 3336875 1.8609127 5.994143 8
CTTTA 3328195 1.8560721 5.0043063 5
CGTGA 2153785 1.8450563 13.386057 9
CCGTG 1722820 1.8400481 5.098865 60-64
TGGCT 2469110 1.8296618 5.053604 9
CTCTG 2111790 1.8198242 6.5999517 20-24
TTCCA 2239835 1.800367 5.168108 75-79
AAGGA 2252970 1.7896068 5.550303 10-14
CACAA 1619295 1.7395016 6.107168 85-89
TATTC 3099460 1.7285109 6.432254 5
CCTTA 2140520 1.7205381 7.144062 95-97
CACCC 1011395 1.6888212 6.068223 50-54
CCCAA 1253845 1.6792862 36.912132 2
CTTTC 2408450 1.6745793 7.324547 7
CCAAT 1791930 1.6651101 25.741884 3
CATCC 1423740 1.6494329 6.504809 90-94
CCTGT 1906490 1.6429079 6.2237635 4
TTCGG 2197360 1.6282893 9.241523 7
GTGGC 1757975 1.6145581 5.77573 8
CCGCC 896355 1.6046363 6.0164495 45-49
GCACC 1116975 1.6038288 5.345536 50-54
CCACA 1193310 1.5982112 8.242724 1
TCGGT 2122200 1.5725943 10.728892 8
TTGGT 3040570 1.5632429 15.344654 7
AGAAA 2072275 1.53538 5.409313 9
GCTTG 2069800 1.5337648 6.535634 1
GAACA 1630795 1.5064332 5.774802 80-84
TCCAA 1610100 1.4961487 5.373007 75-79
GCAAG 1509185 1.4946058 5.318578 1
TGTAG 2501590 1.4868408 5.3824325 9
GCTGA 1732885 1.4844892 5.082459 80-84
TGAAC 1857645 1.4843498 5.284847 80-84
CATGC 1486230 1.4806132 6.09885 2
ACATG 1827605 1.4603465 15.53486 1
TACTT 2616295 1.4590589 7.1364927 3
ACACA 1335635 1.4347844 5.88083 85-89
CTACC 1238035 1.4342898 5.2804523 25-29
GTGTA 2409210 1.431934 9.153127 2
GCAAC 1234125 1.4213192 6.233729 1
GCGGC 1062430 1.406372 5.7607474 7
TGGTA 2357595 1.4012562 7.6695914 2
CTGTA 2007235 1.3873782 6.364566 8
TTAGG 2331745 1.3858919 5.137103 35-39
ACTTT 2455985 1.3696569 7.458592 4
GTAGC 1589595 1.3617387 5.5165277 1
AACAC 1265535 1.3594806 5.891772 85-89
ATGCC 1358560 1.3534257 7.009 9
ACGGT 1579870 1.3534076 6.5808516 4
ATGGG 1835370 1.3520184 13.680225 3
AGCCT 1328040 1.323021 5.4886355 4
GGTCG 1421555 1.3055835 14.026433 6
AAGCC 1120630 1.2906094 6.3596015 1
CAATT 1986225 1.2805349 17.935034 4
GTATT 2667590 1.279254 6.0762568 4
TGGGT 1963695 1.2512846 10.360528 4
GGTAC 1438485 1.2322892 9.100029 3
GACGG 1134435 1.2044742 8.29737 3
TGGCC 1125140 1.2016994 9.685535 1
GCCTG 1124495 1.2010105 7.09374 6
GGGTC 1298900 1.1929348 13.944483 5
TGGTC 1601490 1.1867373 18.02379 8
GGTCA 1361610 1.1664336 20.62704 9
CACAT 1239735 1.1519955 5.430957 2
GCTCA 1150860 1.1465107 5.887791 1
TGTAT 2384210 1.1433581 5.9611416 3
GTACT 1606450 1.1103601 7.3131394 4
TCGTG 1487955 1.1026056 11.458741 8
CTGAC 1102885 1.098717 7.685051 1
ATTCG 1579420 1.0916773 7.835698 6
TGGGG 1381450 1.0910077 5.1653633 9
ATTGG 1791485 1.0647839 15.182274 6
TGACG 1236410 1.05918 6.5592966 2
GGGGT 1293695 1.0217026 5.205577 8
AATTG 1790530 0.9926503 13.713402 5
GGCCA 770930 0.9518776 5.094984 1
GCCCA 636445 0.9138511 5.4856215 2
GGCCT 839115 0.896212 6.8088713 5
CGGTA 994800 0.85220295 6.1388316 5
GCCTA 838055 0.8348878 5.057216 3
CTACA 863245 0.8021508 6.7544007 3
CCCCG 441715 0.7907491 6.3679385 1
GTCGT 1061150 0.7863342 11.211146 7
GGCCC 510645 0.78608096 6.4441843 1
CCCGG 372380 0.5732375 5.2689643 2
Produced by FastQC (version 0.10.1)