##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	ZL7_ATCACG_L008_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	9633027	
Filtered Sequences	0	
Sequence length	101	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.19112777323265	34.0	33.0	34.0	31.0	34.0
2	33.36345418734942	34.0	34.0	34.0	31.0	34.0
3	33.406773281129595	34.0	34.0	34.0	31.0	34.0
4	36.65157348775208	37.0	37.0	37.0	35.0	37.0
5	36.59215737690759	37.0	37.0	37.0	35.0	37.0
6	36.596521114287334	37.0	37.0	37.0	35.0	37.0
7	36.59441066655372	37.0	37.0	37.0	35.0	37.0
8	36.58870020814849	37.0	37.0	37.0	35.0	37.0
9	38.45722263624923	39.0	39.0	39.0	37.0	39.0
10-14	38.7640576736679	39.4	39.2	39.4	37.2	39.4
15-19	40.05395091283353	41.0	40.0	41.0	38.0	41.0
20-24	39.996144368743074	41.0	40.0	41.0	38.0	41.0
25-29	39.86545633060096	41.0	40.0	41.0	38.0	41.0
30-34	39.70277596024594	41.0	40.0	41.0	38.0	41.0
35-39	39.5287017050819	41.0	40.0	41.0	37.0	41.0
40-44	39.335933305284	41.0	39.4	41.0	36.4	41.0
45-49	39.18929900227623	40.4	39.0	41.0	36.0	41.0
50-54	39.13226658660876	40.4	39.0	41.0	35.6	41.0
55-59	38.844008139912816	40.0	38.4	41.0	35.0	41.0
60-64	38.59738281642935	40.0	37.6	41.0	35.0	41.0
65-69	38.03509708838146	39.4	36.6	41.0	34.4	41.0
70-74	37.286817072141496	38.4	35.4	40.4	34.0	41.0
75-79	36.116702776811486	36.4	34.8	39.2	32.8	40.2
80-84	35.69413124244332	35.8	35.0	37.8	33.4	39.4
85-89	34.94786810002712	35.0	35.0	36.6	33.0	38.0
90-94	34.419947125654275	35.0	35.0	35.8	33.0	36.8
95-99	34.08713238320623	35.0	35.0	35.0	32.2	36.0
100-101	33.705184050662375	35.0	34.0	35.0	31.5	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	9.0
13	58.0
14	254.0
15	824.0
16	2041.0
17	3703.0
18	5313.0
19	6636.0
20	7698.0
21	8731.0
22	10440.0
23	12474.0
24	14984.0
25	18180.0
26	22270.0
27	27733.0
28	34649.0
29	44554.0
30	56036.0
31	68334.0
32	90085.0
33	124833.0
34	191581.0
35	330164.0
36	700423.0
37	1836850.0
38	3892221.0
39	2088658.0
40	33287.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.15685269636863	30.86426003062437	30.154545993207755	19.82434127979925
2	20.10068071022743	30.23520021276801	30.405022221986922	19.25909685501764
3	20.408429781407435	29.39006476701419	30.222931820753473	19.978573630824904
4	20.35143933144912	29.179403248038465	30.292201381623652	20.176956038888765
5	20.643085501577023	29.498059125132738	30.198617734591632	19.660237638698614
6	20.635389097575572	29.689800524136057	29.473388079200706	20.201422299087668
7	20.58955092724229	29.943516196933732	29.789016474260894	19.67791640156308
8	20.171333475967625	29.908117147393025	29.81924580923525	20.1013035674041
9	20.155128808421278	29.666593896186527	30.082683252107568	20.095594043284628
10-14	20.124787359155125	29.769375711289918	29.981078637068077	20.12475829248688
15-19	20.11551690643205	29.618399192227912	30.054275223636644	20.21180867770339
20-24	20.249614367407247	29.520013031865282	29.88253756627009	20.347835034457372
25-29	20.420097251677888	29.472240842768983	29.68087724371071	20.426784661842415
30-34	20.518055869233486	29.458055471435195	29.60471556238816	20.41917309694315
35-39	20.621384412569242	29.35859485206954	29.510597141529804	20.509423593831414
40-44	20.641418462203994	29.360188511747374	29.4803772909085	20.51801573514013
45-49	20.708008833973178	29.28619234512456	29.440869093144133	20.564929727758127
50-54	20.634147896075387	29.300652412156065	29.443762242395195	20.62143744937336
55-59	20.70881618627924	29.273913743077074	29.41739568248234	20.599874388161343
60-64	20.64751991986398	29.29464759744647	29.402510072386058	20.65532241030349
65-69	20.655373567783716	29.325828223656075	29.343438827257547	20.675359381302663
70-74	20.702001186269943	29.35396174103959	29.298928085515097	20.645108987175366
75-79	20.698660853896165	29.313499383142073	29.317340988080726	20.67049877488104
80-84	20.715887453604775	29.324228551225673	29.32811121408127	20.631772781088284
85-89	20.677510801681027	29.337627705590315	29.29303856231064	20.691822930418013
90-94	20.687417814758845	29.288972470019313	29.2797324864885	20.743877228733343
95-99	20.70519262915493	29.349244029652827	29.215489831614182	20.73007350957806
100-101	20.799178551566992	29.329523133654273	29.10104714743187	20.770251167346863
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	38.98119397616788
2	39.35977756524507
3	40.38700341223234
4	40.52839537033788
5	40.30332314027564
6	40.836811396663244
7	40.26746732880537
8	40.272637043371724
9	40.25072285170591
10-14	40.249545651642
15-19	40.327325584135444
20-24	40.59744940186462
25-29	40.846881913520306
30-34	40.93722896617664
35-39	41.130808006400656
40-44	41.159434197344126
45-49	41.2729385617313
50-54	41.25558534544874
55-59	41.30869057444059
60-64	41.30284233016747
65-69	41.33073294908638
70-74	41.34711017344531
75-79	41.369159628777204
80-84	41.34766023469306
85-89	41.36933373209904
90-94	41.43129504349219
95-99	41.43526613873299
100-101	41.56942971891386
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	344.0
1	282.0
2	269.0
3	383.0
4	434.5
5	473.0
6	615.5
7	819.5
8	1081.0
9	1274.0
10	1473.5
11	1962.0
12	2698.0
13	3466.5
14	4312.0
15	5638.0
16	7411.5
17	10188.5
18	14659.5
19	20982.0
20	29624.0
21	40471.5
22	53925.0
23	69944.5
24	88409.5
25	109489.0
26	132187.0
27	156457.0
28	182198.5
29	208366.0
30	233855.5
31	258495.0
32	282253.0
33	304850.0
34	326569.5
35	346355.0
36	364199.0
37	378375.0
38	385290.5
39	385476.5
40	379667.5
41	366806.5
42	347483.0
43	325830.0
44	304527.5
45	285888.5
46	271109.5
47	260158.0
48	251133.0
49	240843.0
50	228930.0
51	214649.5
52	198715.5
53	183077.0
54	169131.5
55	157323.5
56	146206.0
57	134450.5
58	121544.0
59	106887.5
60	91748.5
61	77407.0
62	64366.5
63	53315.5
64	44250.0
65	36605.5
66	30194.0
67	24761.5
68	20081.0
69	16131.0
70	12927.0
71	10266.0
72	8069.0
73	6526.0
74	5352.5
75	4406.5
76	3628.5
77	2999.0
78	2494.0
79	2006.5
80	1606.5
81	1252.5
82	931.0
83	662.0
84	432.0
85	268.5
86	173.5
87	118.5
88	84.5
89	58.5
90	38.0
91	29.5
92	22.5
93	19.5
94	15.0
95	12.0
96	12.0
97	5.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13741267412621183
2	0.0
3	3.840952589461236E-4
4	1.3495238827836774E-4
5	0.0
6	3.321904942236744E-4
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	1.3287619768946978E-4
20-24	2.4706668007885786E-4
25-29	4.152381177795931E-6
30-34	4.360000236685727E-5
35-39	1.4740953181175553E-4
40-44	1.5156191298955148E-4
45-49	3.5502859070155203E-4
50-54	6.228571766693896E-5
55-59	0.0018374286711746993
60-64	0.0021301715442093123
65-69	0.002015981061819924
70-74	0.01899299150723859
75-79	0.01698116282659646
80-84	0.004802228832120994
85-89	0.006616819406817815
90-94	0.010123505311466479
95-99	0.007590552793010961
100-101	0.0029689525421240904
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	9633027.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	6.0509331763907825
#Duplication Level	Relative count
1	100.0
2	3.6312285656647165
3	0.7294310013484386
4	0.4910652665555355
5	0.33392438125776414
6	0.21129078496119258
7	0.16988203815975284
8	0.13537474915855302
9	0.09608952783411019
10++	0.37798753490545
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCCAG	1761140	3.6253393	4.0372353	25-29
CTGGG	1707405	3.480361	3.6588728	75-79
CCAGG	1577150	3.23665	3.5069585	25-29
GGAGG	1545245	3.151781	3.4574203	70-74
CCTCC	1519890	3.1267707	3.4907968	70-74
CCTGG	1513110	3.0937865	3.3010347	70-74
>>END_MODULE