##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	ZL7_ATCACG_L007_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	10179575	
Filtered Sequences	0	
Sequence length	101	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.232860900381404	34.0	33.0	34.0	31.0	34.0
2	33.377984640812606	34.0	34.0	34.0	31.0	34.0
3	33.404714047492156	34.0	34.0	34.0	31.0	34.0
4	36.651176105092794	37.0	37.0	37.0	35.0	37.0
5	36.59372979716737	37.0	37.0	37.0	35.0	37.0
6	36.588557184361825	37.0	37.0	37.0	35.0	37.0
7	36.59077328866873	37.0	37.0	37.0	35.0	37.0
8	36.58615069882583	37.0	37.0	37.0	35.0	37.0
9	38.44985139359944	39.0	39.0	39.0	37.0	39.0
10-14	36.868241513029766	39.4	38.4	39.4	26.2	39.4
15-19	39.544645351107484	41.0	39.0	41.0	36.6	41.0
20-24	39.99188577126255	41.0	40.0	41.0	38.0	41.0
25-29	39.86113663880859	41.0	40.0	41.0	38.0	41.0
30-34	39.70619728230304	41.0	40.0	41.0	38.0	41.0
35-39	39.53785345655393	41.0	40.0	41.0	37.0	41.0
40-44	39.34849853751261	41.0	39.4	41.0	36.6	41.0
45-49	39.20141033392848	40.4	39.0	41.0	36.0	41.0
50-54	39.133861050191186	40.4	39.0	41.0	35.6	41.0
55-59	38.8394933580233	40.0	38.4	41.0	35.0	41.0
60-64	38.59049528099159	40.0	37.6	41.0	35.0	41.0
65-69	38.027499203061026	39.4	36.6	41.0	34.4	41.0
70-74	37.253683341396865	38.4	35.4	40.4	34.0	41.0
75-79	36.08195972818119	36.2	34.8	39.2	32.8	40.2
80-84	35.648152540749486	35.8	35.0	37.6	33.2	39.2
85-89	34.92345165687173	35.0	35.0	36.4	33.0	37.8
90-94	34.402729033383025	35.0	35.0	35.8	33.0	36.6
95-99	34.09632156548775	35.0	35.0	35.0	32.4	36.0
100-101	33.734268522998256	35.0	34.0	35.0	31.5	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	10.0
13	63.0
14	261.0
15	820.0
16	2025.0
17	3885.0
18	5527.0
19	6998.0
20	7958.0
21	9039.0
22	10530.0
23	12688.0
24	15605.0
25	18647.0
26	23184.0
27	29124.0
28	36663.0
29	47345.0
30	59512.0
31	75171.0
32	98939.0
33	139209.0
34	219605.0
35	395137.0
36	885509.0
37	2281879.0
38	3934915.0
39	1831208.0
40	28116.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.136112135743268	30.815610475839172	30.208297061354973	19.839980327062584
2	20.107028043901636	30.243669308394505	30.374647271619885	19.27465537608397
3	20.38176446462647	29.393928528450353	30.236596321555666	19.987710685367514
4	20.394672665607356	29.140116360457093	30.281097197083373	20.18411377685218
5	20.657375185113327	29.477321007998857	30.188244597637915	19.677059209249894
6	20.64017407406498	29.652662316452304	29.493520112578373	20.21364349690434
7	20.595496373866297	29.899440791978055	29.812423406674636	19.69263942748101
8	20.15706942578644	29.928665980652436	29.812845821166405	20.101418772394723
9	20.16671619394719	29.62630561688479	30.107563429710964	20.099414759457048
10-14	20.25178990419853	29.818200633418346	29.868399743058866	20.06160971932426
15-19	20.113978517397506	29.590668067092913	30.079325374868095	20.21602804064149
20-24	20.239365665014518	29.50612026741601	29.910318674827757	20.344195392741717
25-29	20.425730936704138	29.453131393010025	29.70281568729539	20.41832198299045
30-34	20.505075879736832	29.444027988805328	29.62500445500784	20.42589167645
35-39	20.607026403233505	29.334999599884625	29.541607666921838	20.516366329960032
40-44	20.64232698225133	29.353995058016668	29.494081706975688	20.50959625275631
45-49	20.694080181020453	29.272352178208827	29.458293527632307	20.575274113138413
50-54	20.616129904151208	29.297767114486522	29.472157848635145	20.613945132727125
55-59	20.69204752679174	29.253280143320996	29.447217890227662	20.6074544396596
60-64	20.642325440895128	29.28153876758116	29.42857437564928	20.647561415874435
65-69	20.656142184737433	29.32217945493448	29.34513393752958	20.676544422798504
70-74	20.692978079839914	29.344067886005327	29.312119375889058	20.650834658265705
75-79	20.700617048329182	29.295420472312212	29.336440435917925	20.66752204344068
80-84	20.705188960981804	29.325684987488525	29.32638050599168	20.642745545537995
85-89	20.678543255397074	29.322865543667152	29.31561130915512	20.682979891780654
90-94	20.671095122463427	29.290578074915402	29.29014187322454	20.74818492939663
95-99	20.69745592757787	29.33303688336299	29.239783248073515	20.72972394098563
100-101	20.794064188666958	29.30286665472397	29.12037132320634	20.782697833402732
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	38.97609246280585
2	39.381683419985606
3	40.36947514999398
4	40.57878644245954
5	40.33443439436322
6	40.85381757096932
7	40.28813580134731
8	40.25848819818116
9	40.26613095340424
10-14	40.31339962352279
15-19	40.330006558039
20-24	40.583561057756235
25-29	40.84405291969458
30-34	40.93096755618683
35-39	41.12339273319353
40-44	41.15192323500764
45-49	41.269354294158866
50-54	41.23007503687833
55-59	41.299501966451345
60-64	41.289886856769556
65-69	41.33268660753593
70-74	41.34381273810562
75-79	41.36813909176986
80-84	41.3479345065198
85-89	41.36152314717773
90-94	41.419280051860056
95-99	41.4271798685635
100-101	41.576762022069694
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	314.0
1	276.5
2	282.5
3	383.0
4	460.5
5	509.5
6	655.5
7	866.0
8	1097.5
9	1309.5
10	1581.5
11	2088.5
12	2770.5
13	3586.5
14	4612.0
15	5981.0
16	7844.5
17	10790.5
18	15448.0
19	22228.0
20	31548.0
21	43261.0
22	57431.5
23	74288.0
24	93924.5
25	115949.0
26	140191.0
27	166607.5
28	193891.0
29	221739.0
30	249432.5
31	275039.5
32	299600.5
33	324186.5
34	347021.5
35	367583.0
36	386385.5
37	400982.0
38	408319.0
39	407398.0
40	400282.5
41	386943.5
42	367142.0
43	344034.0
44	321203.0
45	301711.5
46	285896.0
47	273955.0
48	264311.5
49	253426.0
50	240448.5
51	225834.5
52	209229.0
53	192631.5
54	178203.5
55	165450.5
56	153503.0
57	141415.5
58	127491.5
59	112152.5
60	96560.0
61	81078.0
62	67171.5
63	55755.5
64	46230.0
65	37997.0
66	31391.0
67	25921.0
68	20985.5
69	16785.0
70	13297.0
71	10547.5
72	8511.0
73	6867.0
74	5564.0
75	4549.0
76	3779.0
77	3203.0
78	2629.0
79	2097.5
80	1658.0
81	1251.0
82	903.5
83	660.0
84	423.0
85	254.0
86	165.5
87	103.0
88	68.0
89	44.0
90	32.0
91	25.5
92	26.0
93	23.5
94	16.5
95	10.0
96	6.5
97	7.5
98	4.5
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13089937448272643
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	2.4511671656233194
15-19	7.465930552110477E-5
20-24	1.9450713806814135E-4
25-29	0.0
30-34	1.9057770093545162E-4
35-39	1.1002423971531229E-4
40-44	1.7878938953738244E-4
45-49	0.0012633140381597464
50-54	2.0040129376717593E-4
55-59	1.9450713806814135E-4
60-64	0.0
65-69	0.0029431484123846035
70-74	0.019142252991897993
75-79	0.015757042902085794
80-84	0.001169007546975193
85-89	0.006803820395252257
90-94	0.007833332924016966
95-99	0.004463840582735527
100-101	0.004032584857422829
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.0179575E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	5.752761914262207
#Duplication Level	Relative count
1	100.0
2	3.423457750541117
3	0.7514778181741206
4	0.4530035298289065
5	0.33498975979170303
6	0.20586258540122035
7	0.16299659718142898
8	0.11166325326390102
9	0.08784881536401691
10++	0.3106461121607104
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCCAG	1846500	3.6219807	3.9670706	25-29
CTGGG	1795585	3.4872112	3.7145002	85-89
CCAGG	1648890	3.2260041	3.4644809	25-29
GGAGG	1616910	3.1471121	3.4359295	70-74
CCTCC	1595315	3.1224046	3.4366083	70-74
CCTGG	1587435	3.090948	3.275654	3
>>END_MODULE