##FastQC 0.10.1 >>Basic Statistics pass #Measure Value Filename ZL6_CTTGTA_L008_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11657818 Filtered Sequences 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.20227018469494 34.0 33.0 34.0 31.0 34.0 2 33.37215523522498 34.0 34.0 34.0 31.0 34.0 3 33.41520960440453 34.0 34.0 34.0 31.0 34.0 4 36.65825131255266 37.0 37.0 37.0 35.0 37.0 5 36.59879473156984 37.0 37.0 37.0 35.0 37.0 6 36.60306465583868 37.0 37.0 37.0 35.0 37.0 7 36.60194695096458 37.0 37.0 37.0 35.0 37.0 8 36.59389698827002 37.0 37.0 37.0 35.0 37.0 9 38.463545579455776 39.0 39.0 39.0 37.0 39.0 10-14 38.771599247818074 39.4 39.2 39.4 37.2 39.4 15-19 40.06452337821709 41.0 40.0 41.0 38.0 41.0 20-24 40.00652926645449 41.0 40.0 41.0 38.0 41.0 25-29 39.87611753760438 41.0 40.0 41.0 38.0 41.0 30-34 39.71545036987196 41.0 40.0 41.0 38.0 41.0 35-39 39.54025861443368 41.0 40.0 41.0 37.0 41.0 40-44 39.35056189760382 41.0 39.4 41.0 36.6 41.0 45-49 39.200138430708044 40.4 39.0 41.0 36.0 41.0 50-54 39.13825336782578 40.6 39.0 41.0 35.6 41.0 55-59 38.8485831739696 40.0 38.4 41.0 35.0 41.0 60-64 38.60266571325784 40.0 37.6 41.0 35.0 41.0 65-69 38.041153893464454 39.4 36.6 41.0 34.4 41.0 70-74 37.29930053805952 38.4 35.4 40.4 34.0 41.0 75-79 36.131735578647735 36.4 34.8 39.2 33.0 40.2 80-84 35.70646630441477 35.8 35.0 37.8 33.4 39.4 85-89 34.96054292492814 35.0 35.0 36.6 33.0 38.0 90-94 34.434110688638306 35.0 35.0 35.8 33.0 36.8 95-99 34.101858615394406 35.0 35.0 35.0 32.2 36.0 100-101 33.72289853041109 35.0 34.0 35.0 31.5 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 15.0 13 61.0 14 325.0 15 971.0 16 2278.0 17 4296.0 18 6412.0 19 8017.0 20 9312.0 21 10593.0 22 12607.0 23 14962.0 24 17778.0 25 21596.0 26 26451.0 27 32381.0 28 40834.0 29 51640.0 30 64522.0 31 82508.0 32 107592.0 33 148492.0 34 230330.0 35 399794.0 36 848758.0 37 2225315.0 38 4720780.0 39 2529336.0 40 39860.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 19.199432521132927 31.040236763343255 29.504752197430147 20.25557851809367 2 20.265061609299444 30.20739387079126 30.435172345287942 19.092372174621357 3 20.3409847720369 29.282485046017403 30.22162740642202 20.154902775523674 4 20.26326220703431 29.182486894833655 30.31329134201656 20.240959556115484 5 20.568248706576135 29.514065153530446 30.178897972159113 19.738788167734306 6 20.554515097159502 29.636168573646344 29.542746055877316 20.266570273316837 7 20.643502926533934 29.870692783160624 29.760912376569955 19.724891913735487 8 20.122848032110298 29.775117436213193 29.912879065361974 20.18915546631454 9 20.102921490110756 29.57681274488931 30.187012698259657 20.133253066740277 10-14 20.15447487685946 29.723627526180284 29.975945755886734 20.14595184107352 15-19 20.173934686757224 29.571610811136495 30.01109677526496 20.243357726841317 20-24 20.29527837466955 29.458670037823264 29.86160543921532 20.384446148291865 25-29 20.471345495357706 29.438466100603044 29.649951646182842 20.4402367578564 30-34 20.56403471305908 29.405233969070565 29.57012937176286 20.460601946107495 35-39 20.636910555389342 29.328756209083085 29.50534012411707 20.528993111410504 40-44 20.66098219075269 29.346775699392154 29.45933373002309 20.532908379832065 45-49 20.71609438001195 29.264492808012516 29.409077983644377 20.61033482833116 50-54 20.65518439687889 29.29711196558064 29.42686187952675 20.620841758013718 55-59 20.719873189713645 29.242462121201722 29.412646331966418 20.62501835711821 60-64 20.667101657054662 29.269075467115137 29.387866890608027 20.675955985222174 65-69 20.703478913252 29.24941536023777 29.3414102346571 20.70569549185313 70-74 20.740880580543784 29.28794660299611 29.301147050792093 20.67002576566801 75-79 20.729881229790763 29.25058612103482 29.332942914852595 20.686589734321824 80-84 20.733433867025685 29.26617496019302 29.335427983512545 20.664963189268747 85-89 20.696260873215937 29.263139542111627 29.31285895657789 20.727740628094544 90-94 20.699542886046135 29.221531951136402 29.301855461947262 20.7770697008702 95-99 20.710776424766742 29.267380713715802 29.25452137453794 20.76732148697952 100-101 20.820261769682876 29.216557995923896 29.152577514409977 20.810602719983248 >>END_MODULE >>Per base GC content pass #Base %GC 1 39.4550110392266 2 39.3574337839208 3 40.49588754756058 4 40.50422176314979 5 40.30703687431044 6 40.82108537047634 7 40.368394840269424 8 40.312003498424836 9 40.23617455685103 10-14 40.30042671793298 15-19 40.417292413598545 20-24 40.67972452296142 25-29 40.91158225321411 30-34 41.024636659166575 35-39 41.16590366679985 40-44 41.193890570584756 45-49 41.32642920834311 50-54 41.276026154892605 55-59 41.34489154683186 60-64 41.34305764227684 65-69 41.409174405105134 70-74 41.410906346211796 75-79 41.416470964112584 80-84 41.39839705629443 85-89 41.42400150131048 90-94 41.47661258691633 95-99 41.47809791174626 100-101 41.63086448966612 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 436.0 1 369.5 2 320.0 3 420.0 4 532.5 5 600.5 6 758.5 7 998.5 8 1330.0 9 1585.0 10 1908.0 11 2552.0 12 3391.0 13 4343.5 14 5394.5 15 6935.0 16 9110.0 17 12084.0 18 16663.5 19 23835.0 20 33960.0 21 46771.5 22 62811.0 23 81919.5 24 104312.0 25 129656.5 26 158127.5 27 189209.5 28 220783.5 29 252203.0 30 283087.5 31 313260.5 32 342006.5 33 370878.5 34 397901.0 35 421684.5 36 443951.0 37 460793.5 38 469324.0 39 468663.5 40 458955.0 41 440635.0 42 416080.0 43 388737.5 44 362615.0 45 342192.0 46 326908.5 47 314795.0 48 304231.5 49 292497.0 50 277767.0 51 260250.5 52 241441.0 53 222792.5 54 206099.5 55 191318.0 56 177648.5 57 163938.5 58 148162.5 59 130620.5 60 112635.5 61 94874.5 62 78613.5 63 65429.5 64 54343.5 65 44919.0 66 37166.5 67 30456.5 68 24772.0 69 19967.0 70 15904.5 71 12749.0 72 10230.5 73 8239.0 74 6794.5 75 5659.5 76 4625.0 77 3924.0 78 3408.0 79 2720.5 80 2119.0 81 1699.0 82 1326.0 83 961.0 84 656.0 85 412.0 86 251.0 87 166.0 88 119.0 89 82.0 90 62.0 91 49.0 92 38.0 93 30.5 94 24.5 95 18.0 96 14.0 97 9.0 98 5.5 99 3.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13854222119439502 2 0.0 3 4.632084666272882E-4 4 9.435728023889205E-5 5 0.0 6 2.487601024479881E-4 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 1.4410930072848968E-4 20-24 1.612651698628337E-4 25-29 0.0 30-34 3.0880564441819214E-5 35-39 1.5268723529566168E-4 40-44 7.891699801798244E-5 45-49 4.546305320601162E-4 50-54 5.832995505676963E-5 55-59 0.0018425403450285465 60-64 0.002079291339082494 65-69 0.0018185221282404648 70-74 0.018466577536207892 75-79 0.01671839447141824 80-84 0.004848248617365617 85-89 0.006881219109785381 90-94 0.010344989088009437 95-99 0.007895130975625112 100-101 0.00305803367319682 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.1657818E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Duplicate Percentage 6.485561305196132 #Duplication Level Relative count 1 100.0 2 3.9007054353742223 3 0.7342504348939712 4 0.46851154203263573 5 0.3569865849884206 6 0.2572010971067545 7 0.20117181246731625 8 0.14994503793982988 9 0.11365940598286038 10++ 0.5400155814772521 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Kmer Content warn #Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position CCCAG 2134500 3.6142223 3.949003 25-29 CTGGG 2081880 3.488877 3.665481 85-89 CCAGG 1906340 3.2198906 3.4292421 25-29 GGAGG 1878730 3.1575427 3.4109364 70-74 CCTCC 1849810 3.1231363 3.4066048 65-69 CCTGG 1843820 3.0976079 3.3126361 3 >>END_MODULE