##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	ZL6_CTTGTA_L007_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	12370038	
Filtered Sequences	0	
Sequence length	101	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.241333050068235	34.0	33.0	34.0	31.0	34.0
2	33.384769149456126	34.0	34.0	34.0	31.0	34.0
3	33.411169230037935	34.0	34.0	34.0	31.0	34.0
4	36.65668035942978	37.0	37.0	37.0	35.0	37.0
5	36.59961739810338	37.0	37.0	37.0	35.0	37.0
6	36.59234231940112	37.0	37.0	37.0	35.0	37.0
7	36.59665572571402	37.0	37.0	37.0	35.0	37.0
8	36.591729305924524	37.0	37.0	37.0	35.0	37.0
9	38.45643238929419	39.0	39.0	39.0	37.0	39.0
10-14	36.87952090365446	39.4	38.4	39.4	26.2	39.4
15-19	39.555593976348334	41.0	39.0	41.0	36.6	41.0
20-24	40.000191591974094	41.0	40.0	41.0	38.0	41.0
25-29	39.87028245184049	41.0	40.0	41.0	38.0	41.0
30-34	39.71480008388009	41.0	40.0	41.0	38.0	41.0
35-39	39.54661199909007	41.0	40.0	41.0	37.0	41.0
40-44	39.359281758067354	41.0	39.4	41.0	36.6	41.0
45-49	39.20804356461961	40.4	39.0	41.0	36.0	41.0
50-54	39.1375524957967	40.4	39.0	41.0	35.6	41.0
55-59	38.842825688975246	40.0	38.4	41.0	35.0	41.0
60-64	38.592898744530935	40.0	37.6	41.0	35.0	41.0
65-69	38.02915109880827	39.4	36.6	41.0	34.4	41.0
70-74	37.2618857112646	38.4	35.2	40.4	34.0	41.0
75-79	36.0936545223224	36.2	34.8	39.2	33.0	40.2
80-84	35.65702381835852	35.8	35.0	37.6	33.4	39.2
85-89	34.93262702992505	35.0	35.0	36.4	33.0	37.8
90-94	34.412617794706854	35.0	35.0	35.8	33.0	36.6
95-99	34.107343518265665	35.0	35.0	35.0	32.4	36.0
100-101	33.74629318034431	35.0	34.5	35.0	31.5	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	13.0
13	76.0
14	279.0
15	1026.0
16	2503.0
17	4626.0
18	6813.0
19	8440.0
20	9813.0
21	11123.0
22	12906.0
23	15360.0
24	18430.0
25	22472.0
26	27743.0
27	34514.0
28	43157.0
29	54721.0
30	69956.0
31	90104.0
32	119190.0
33	168306.0
34	264972.0
35	480405.0
36	1077445.0
37	2772567.0
38	4796630.0
39	2222536.0
40	33911.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.149697592599594	30.99180253622822	29.594463973907736	20.264035897264453
2	20.270131748988966	30.201338104216006	30.43487821136847	19.093651935426553
3	20.315572191451636	29.2793199180148	30.25570333737051	20.149404553163052
4	20.2671244825602	29.19813989253711	30.316891508336514	20.217844116566177
5	20.575791325442278	29.51358997551907	30.184436796753317	19.726181902285337
6	20.55054317537262	29.63163896505411	29.563579351979357	20.254238507593914
7	20.628424908638117	29.87911597361301	29.750708930724386	19.741750187024486
8	20.12646202056938	29.790336941568007	29.92385310376573	20.15934793409689
9	20.10659142680079	29.55889868729587	30.19841976233218	20.136090123571165
10-14	20.26680093656402	29.786626695024193	29.857824318930888	20.0887480494809
15-19	20.153733190984973	29.570330507233745	30.03785892559927	20.23807737618202
20-24	20.279859484001296	29.457011336726886	29.875643247371276	20.387485931900546
25-29	20.45970109388508	29.420800485819044	29.670141676201805	20.44935674409408
30-34	20.564671153979138	29.406086364922977	29.585688375830415	20.443554105267467
35-39	20.63385240976459	29.323219613456452	29.528724509260357	20.514203467518602
40-44	20.665073868478903	29.32387359611701	29.47850561572598	20.532546919678108
45-49	20.68482608269722	29.259329316031646	29.45134601261269	20.604498588658444
50-54	20.62953515985751	29.30041157586215	29.45713085577254	20.612922408507796
55-59	20.704632274497182	29.249605432804195	29.415336912915574	20.630425379783052
60-64	20.648318137745413	29.27733770906767	29.403393910350157	20.670950242836764
65-69	20.687633484879946	29.248849002261128	29.359540813225628	20.703976699633298
70-74	20.7201543047106	29.286940019758674	29.330167370428395	20.662738305102334
75-79	20.72051258462764	29.24942362301759	29.352144881751535	20.677918910603236
80-84	20.732147134818437	29.261571472770996	29.360264516305946	20.64601687610462
85-89	20.683194718344904	29.27446204279307	29.33537001280524	20.70697322605679
90-94	20.68085888571023	29.23746604053853	29.315797274162325	20.765877799588914
95-99	20.69466652177194	29.26705931283778	29.281304096526533	20.756970068863748
100-101	20.793477729671537	29.224233177154783	29.17573484094522	20.806554252228455
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	39.41373348986405
2	39.36378368441552
3	40.46497674461469
4	40.484968599126375
5	40.30197322772761
6	40.804781682966535
7	40.3701750956626
8	40.28580995466627
9	40.242681550371955
10-14	40.35554898604492
15-19	40.39181056716699
20-24	40.66734541590184
25-29	40.909057837979155
30-34	41.00822525924661
35-39	41.14805587728319
40-44	41.19762078815701
45-49	41.289324671355665
50-54	41.24245756836531
55-59	41.33505765428024
60-64	41.31926838058218
65-69	41.39161018451325
70-74	41.382892609812934
75-79	41.398431495230874
80-84	41.37816401092306
85-89	41.39016794440169
90-94	41.44673668529914
95-99	41.45163659063569
100-101	41.600031981899996
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	409.0
1	348.5
2	328.5
3	444.0
4	554.5
5	610.0
6	760.5
7	1041.0
8	1384.0
9	1676.5
10	1972.5
11	2592.0
12	3590.5
13	4562.0
14	5596.0
15	7259.5
16	9626.5
17	12823.5
18	17912.0
19	25822.5
20	36494.5
21	50133.0
22	67169.5
23	87694.0
24	111766.5
25	139440.0
26	169893.0
27	201814.5
28	235051.5
29	268958.5
30	302494.0
31	334239.5
32	365754.0
33	396216.0
34	423840.5
35	449526.5
36	473125.5
37	491789.0
38	499867.0
39	497823.0
40	487022.5
41	467016.0
42	440569.0
43	411732.0
44	384513.5
45	362749.5
46	346121.0
47	332848.5
48	322149.5
49	309986.0
50	293570.5
51	275114.5
52	255611.5
53	235700.5
54	217332.5
55	201499.5
56	187080.0
57	172116.0
58	155572.5
59	136981.0
60	117459.0
61	98918.0
62	82397.0
63	68351.5
64	56638.0
65	46805.0
66	38781.5
67	31857.5
68	25794.0
69	20646.5
70	16389.5
71	13111.0
72	10652.5
73	8628.5
74	6963.5
75	5766.5
76	4851.0
77	4076.0
78	3436.0
79	2821.0
80	2240.5
81	1783.0
82	1346.0
83	943.5
84	654.5
85	430.0
86	249.5
87	144.5
88	112.5
89	87.0
90	55.5
91	37.0
92	29.5
93	30.0
94	23.0
95	13.5
96	10.5
97	7.5
98	5.0
99	4.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13208528542919595
2	0.0
3	0.0
4	0.0
5	8.084049539702302E-6
6	0.0
7	0.0
8	0.0
9	0.0
10-14	2.4318033622855486
15-19	7.437325576526118E-5
20-24	2.4413829609900957E-4
25-29	0.0
30-34	1.6653142051786744E-4
35-39	1.3257841245111778E-4
40-44	2.0695166821637896E-4
45-49	0.0014244095288955458
50-54	1.7784908987345067E-4
55-59	2.2958700692754543E-4
60-64	0.0
65-69	0.003060621155731292
70-74	0.01944375595289198
75-79	0.016202052087471355
80-84	0.0013678211821176298
85-89	0.007154383842636538
90-94	0.008291001207918682
95-99	0.004832644814834037
100-101	0.004377512825748797
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.2370038E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	6.145878549297257
#Duplication Level	Relative count
1	100.0
2	3.661175169715431
3	0.7197954377515164
4	0.5098108011844197
5	0.3157743648629252
6	0.2578292373039036
7	0.1796830561004524
8	0.13928094881159328
9	0.09303116809934665
10++	0.4449547868523037
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCCAG	2247125	3.6143985	3.9720042	25-29
CTGGG	2192045	3.4878862	3.7253902	1
CCAGG	2012990	3.2284706	3.458472	25-29
GGAGG	1978945	3.1555989	3.416446	70-74
CCTCC	1945390	3.1223402	3.4202242	95-97
CCTGG	1939770	3.0953987	3.3414056	3
>>END_MODULE