##FastQC 0.10.1 >>Basic Statistics pass #Measure Value Filename ZL5_CAGATC_L008_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 30601475 Filtered Sequences 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.18778395485838 34.0 33.0 34.0 31.0 34.0 2 33.360037775956876 34.0 34.0 34.0 31.0 34.0 3 33.402780944382584 34.0 34.0 34.0 31.0 34.0 4 36.649311609979584 37.0 37.0 37.0 35.0 37.0 5 36.58747370837517 37.0 37.0 37.0 35.0 37.0 6 36.591100853798714 37.0 37.0 37.0 35.0 37.0 7 36.58881223862575 37.0 37.0 37.0 35.0 37.0 8 36.58196900639594 37.0 37.0 37.0 35.0 37.0 9 38.4449490098108 39.0 39.0 39.0 37.0 39.0 10-14 38.751562844601445 39.4 39.2 39.4 37.2 39.4 15-19 40.038635843533676 41.0 40.0 41.0 38.0 41.0 20-24 39.979680077512604 41.0 40.0 41.0 38.0 41.0 25-29 39.84664879062202 41.0 40.0 41.0 38.0 41.0 30-34 39.6852634717771 41.0 40.0 41.0 37.8 41.0 35-39 39.50842376061938 41.0 40.0 41.0 37.0 41.0 40-44 39.317520211035585 41.0 39.2 41.0 36.4 41.0 45-49 39.1704345035656 40.0 39.0 41.0 36.0 41.0 50-54 39.11037479729326 40.4 39.0 41.0 35.4 41.0 55-59 38.82339587879342 40.0 38.4 41.0 35.0 41.0 60-64 38.58153051119268 40.0 37.6 41.0 35.0 41.0 65-69 38.02214937678658 39.4 36.6 41.0 34.4 41.0 70-74 37.28033479431955 38.4 35.4 40.4 34.0 41.0 75-79 36.11590656986305 36.4 34.8 39.2 32.8 40.2 80-84 35.69561008415444 35.8 35.0 37.8 33.2 39.4 85-89 34.94414150298311 35.0 35.0 36.6 33.0 38.0 90-94 34.414368882545695 35.0 35.0 35.8 33.0 36.8 95-99 34.07900068869229 35.0 34.8 35.0 32.0 36.0 100-101 33.69572288590665 35.0 34.0 35.0 31.5 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 0.0 9 1.0 10 2.0 11 13.0 12 37.0 13 163.0 14 853.0 15 2612.0 16 6373.0 17 11995.0 18 17410.0 19 21448.0 20 24665.0 21 28480.0 22 33050.0 23 38803.0 24 47593.0 25 57397.0 26 70504.0 27 87901.0 28 108852.0 29 137900.0 30 174495.0 31 220566.0 32 290819.0 33 402439.0 34 619642.0 35 1069670.0 36 2256636.0 37 5881914.0 38 1.2379857E7 39 6509982.0 40 99401.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 18.957244640192243 31.022634258855987 30.36213924115995 19.657981859791825 2 20.026531400855678 30.329792926648143 30.509728697718003 19.133946974778176 3 20.299503985594875 29.429891754047677 30.321104681433937 19.949499578923515 4 20.32755536654473 29.236517178842213 30.407260159348148 20.028667295264906 5 20.56450546909912 29.571914425693535 30.215808224930335 19.647771880277013 6 20.549625582660987 29.73900160299782 29.545366759849724 20.16600605449147 7 20.551888430214557 29.928354760677383 29.86443954090448 19.655317268203575 8 20.1501398216916 29.918593793273036 29.86157693379159 20.06968945124377 9 20.095201293401708 29.696437835104355 30.137298937387826 20.07106193410612 10-14 20.086739609773712 29.796574838304362 30.03881937063491 20.077866181287014 15-19 20.096137190323674 29.634204215363074 30.090127929686123 20.17953066462713 20-24 20.23944992932779 29.492787664896834 29.925357778978405 20.342404626796966 25-29 20.417060689075818 29.46755336729788 29.712517381188665 20.402868562437632 30-34 20.543033729730904 29.426052718207064 29.611124246284465 20.419789305777563 35-39 20.628837062806685 29.345114158080865 29.53462553725668 20.49142324185577 40-44 20.66719418429494 29.32769919357198 29.47743985056286 20.52766677157022 45-49 20.688896269969266 29.270449656916902 29.438338917322298 20.60231515579153 50-54 20.652789292933623 29.289255624939543 29.442788179849394 20.615166902277444 55-59 20.713522884581607 29.252513750128777 29.408790131081513 20.6251732342081 60-64 20.679194250165466 29.250474870198744 29.39498555056591 20.675345329069877 65-69 20.694295035420684 29.249364852703252 29.351678725928526 20.70466138594754 70-74 20.744520668128335 29.26676413953193 29.311495473745257 20.67721971859448 75-79 20.73481836241108 29.22981516462616 29.34534474962257 20.69002172334019 80-84 20.753442606330218 29.253964586988484 29.326771160053273 20.66582164662803 85-89 20.70652052391605 29.244875518671794 29.3149214276709 20.73368252974126 90-94 20.694159758999618 29.20481434197224 29.30566539520767 20.795360503820472 95-99 20.703590715174975 29.232972140664653 29.274305372225957 20.78913177193442 100-101 20.781521352219404 29.182780710417955 29.195787030656273 20.83991090670637 >>END_MODULE >>Per base GC content pass #Base %GC 1 38.61522649998406 2 39.16047837563386 3 40.24900356451839 4 40.35622266180964 5 40.21227734937614 6 40.715631637152455 7 40.20720569841813 8 40.21982927293538 9 40.16626322750783 10-14 40.16460579106072 15-19 40.275667854950804 20-24 40.58185455612476 25-29 40.81992925151345 30-34 40.962823035508464 35-39 41.12026030466245 40-44 41.19486095586516 45-49 41.2912114257608 50-54 41.26795619521106 55-59 41.338696118789706 60-64 41.35453957923534 65-69 41.39895642136822 70-74 41.42174038672281 75-79 41.42484008575127 80-84 41.41926425295825 85-89 41.44020305365731 90-94 41.48952026282009 95-99 41.4927224871094 100-101 41.62143225892577 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 374.0 1 333.0 2 356.5 3 548.0 4 774.0 5 991.0 6 1431.0 7 2075.0 8 2888.5 9 3574.0 10 4254.5 11 5516.0 12 7398.0 13 9520.5 14 11796.0 15 15323.0 16 20562.5 17 28644.0 18 41551.5 19 60473.0 20 86944.5 21 121237.0 22 164142.5 23 215938.0 24 275839.5 25 344364.5 26 420200.0 27 500354.0 28 583320.0 29 667771.5 30 749080.5 31 827194.0 32 904896.0 33 979285.5 34 1047488.0 35 1112524.0 36 1172380.5 37 1217524.5 38 1240500.5 39 1238929.0 40 1213272.0 41 1165037.5 42 1099857.5 43 1027906.0 44 958162.5 45 900775.5 46 856129.0 47 820652.5 48 791361.0 49 760484.0 50 723523.0 51 678181.0 52 628053.5 53 579713.0 54 536484.5 55 497831.0 56 461950.5 57 425245.5 58 384017.5 59 338573.0 60 292226.0 61 246173.0 62 204102.5 63 169400.5 64 140514.0 65 116505.0 66 96196.0 67 78682.5 68 64148.0 69 51541.5 70 40792.0 71 32477.0 72 26215.0 73 21171.5 74 17154.5 75 14002.0 76 11537.0 77 9679.5 78 8131.0 79 6610.0 80 5245.0 81 4049.0 82 3038.0 83 2200.5 84 1496.5 85 948.5 86 584.0 87 367.5 88 240.5 89 177.5 90 145.5 91 106.0 92 70.5 93 58.5 94 49.5 95 41.0 96 32.0 97 15.5 98 13.5 99 11.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13566993094287122 2 0.0 3 4.7710118548207237E-4 4 1.731942659626701E-4 5 0.0 6 1.9933679667401651E-4 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 1.300590902889485E-4 20-24 1.7776920883715572E-4 25-29 1.960689803350982E-6 30-34 3.0717473585832054E-5 35-39 1.333269066278668E-4 40-44 1.0849150245208768E-4 45-49 3.69916809565552E-4 50-54 5.6206441029394825E-5 55-59 0.0018352056559365192 60-64 0.0022606753432636824 65-69 0.0017705028924259372 70-74 0.017884105259632094 75-79 0.01628418238009769 80-84 0.004871007034791624 85-89 0.006910777993544429 90-94 0.010076638462688482 95-99 0.007736228400755192 100-101 0.003161612307903459 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3.0601475E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Duplicate Percentage 8.149852283615452 #Duplication Level Relative count 1 100.0 2 4.455781758515211 3 0.5829842491021608 4 0.4319407996609672 5 0.3526158226163116 6 0.33305623923543765 7 0.2917637854313703 8 0.24612475754266433 9 0.21895866951367268 10++ 1.7511260343488018 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Kmer Content warn #Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position CCCAG 5640940 3.653993 4.0252304 25-29 CTGGG 5471895 3.507325 3.6904368 85-89 CCAGG 5050345 3.2638795 3.5433872 25-29 GGAGG 4898370 3.151071 3.4241838 70-74 CCTCC 4829150 3.1168692 3.4617405 70-74 CCTGG 4843005 3.1114037 3.2908351 70-74 >>END_MODULE