##FastQC 0.10.1 >>Basic Statistics pass #Measure Value Filename ZL5_CAGATC_L007_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 31252929 Filtered Sequences 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.226413562709595 34.0 33.0 34.0 31.0 34.0 2 33.372590453841944 34.0 34.0 34.0 31.0 34.0 3 33.39940624445152 34.0 34.0 34.0 31.0 34.0 4 36.64832428986096 37.0 37.0 37.0 35.0 37.0 5 36.588141898636124 37.0 37.0 37.0 35.0 37.0 6 36.581662473939645 37.0 37.0 37.0 35.0 37.0 7 36.58458351215657 37.0 37.0 37.0 35.0 37.0 8 36.580214481657066 37.0 37.0 37.0 35.0 37.0 9 38.4378861258092 39.0 39.0 39.0 37.0 39.0 10-14 36.8565466487957 39.4 38.4 39.4 26.2 39.4 15-19 39.529772694264906 41.0 39.0 41.0 36.6 41.0 20-24 39.974855470346476 41.0 40.0 41.0 38.0 41.0 25-29 39.84254796086473 41.0 40.0 41.0 38.0 41.0 30-34 39.68645933953902 41.0 40.0 41.0 37.8 41.0 35-39 39.51777302536988 41.0 40.0 41.0 37.0 41.0 40-44 39.32948868248477 41.0 39.2 41.0 36.4 41.0 45-49 39.17974529683282 40.0 39.0 41.0 36.0 41.0 50-54 39.11189963027145 40.0 39.0 41.0 35.6 41.0 55-59 38.81803185231055 40.0 38.4 41.0 35.0 41.0 60-64 38.57303311315237 40.0 37.6 41.0 35.0 41.0 65-69 38.0114787193226 39.4 36.6 41.0 34.4 41.0 70-74 37.24395401787782 38.4 35.4 40.4 34.0 41.0 75-79 36.078069226727514 36.2 34.8 39.2 32.8 40.2 80-84 35.64658522725982 35.8 35.0 37.6 33.2 39.2 85-89 34.91748578189264 35.0 35.0 36.4 33.0 37.8 90-94 34.3937603288319 35.0 35.0 35.8 33.0 36.6 95-99 34.085590793746086 35.0 34.8 35.0 32.2 36.0 100-101 33.72131124094001 35.0 34.0 35.0 31.5 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 2.0 11 7.0 12 29.0 13 177.0 14 813.0 15 2601.0 16 6514.0 17 12325.0 18 17550.0 19 21803.0 20 24529.0 21 28498.0 22 32987.0 23 38654.0 24 46955.0 25 58093.0 26 70945.0 27 88546.0 28 111917.0 29 142535.0 30 180979.0 31 232904.0 32 309087.0 33 436397.0 34 685813.0 35 1232284.0 36 2756412.0 37 7053230.0 38 1.2067666E7 39 5512853.0 40 79824.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 18.91476322941288 30.974480310968396 30.42737649651138 19.683379963107345 2 20.037872290305973 30.33666380517487 30.509294024889634 19.11616987962952 3 20.311789016639047 29.408782773608195 30.33060677288839 19.948821436864367 4 20.342557332786313 29.245354891376742 30.38898210148559 20.023105674351356 5 20.56997921698795 29.54551555791779 30.250761456630194 19.63374376846407 6 20.56463891752354 29.73474262204352 29.545153991806654 20.155464468626285 7 20.540372391976444 29.927278176071116 29.869555586294005 19.662793845658435 8 20.149749804250348 29.895341969387896 29.889553711909688 20.065354514452068 9 20.08978102500409 29.702166475340597 30.15313860662468 20.054913893030633 10-14 20.21740325631574 29.851933842582 29.91690843041391 20.01375447068835 15-19 20.088090510945467 29.63088725511201 30.09954007213308 20.18148216180944 20-24 20.237484733874812 29.49136755991497 29.93096707528981 20.340180630920404 25-29 20.421788946565616 29.46833111226151 29.713104330157343 20.39677561101553 30-34 20.54159965362286 29.42975977746019 29.622993225953504 20.40564734296345 35-39 20.627073192430384 29.349102449016584 29.538227373268384 20.485596985284648 40-44 20.670396845949607 29.33370997716888 29.475172835178988 20.520720341702532 45-49 20.693639646124105 29.263810499377463 29.45063321910232 20.591916635396117 50-54 20.65078073332751 29.295178156033625 29.44437211687312 20.609668993765744 55-59 20.705900418866683 29.257493326417872 29.418112248161332 20.618494006554116 60-64 20.667474718929544 29.26124140236584 29.403042511631472 20.668241367073147 65-69 20.68627300966407 29.257144670238933 29.36091150834365 20.69567081175335 70-74 20.73900976763965 29.273089603622527 29.31795331827217 20.669947310465655 75-79 20.723488956279677 29.2372798042277 29.352521530234803 20.686709709257826 80-84 20.743343437921133 29.257627683251798 29.336974842547658 20.662054036279407 85-89 20.702606387433427 29.244490560885495 29.33076945240084 20.722133599280237 90-94 20.69246255161582 29.206001469344372 29.321352951368908 20.780183027670905 95-99 20.700107068199657 29.237791558485664 29.285027077179198 20.777074296135478 100-101 20.77044112146626 29.193693458606262 29.211204589493818 20.824660830433665 >>END_MODULE >>Per base GC content pass #Base %GC 1 38.598143192520226 2 39.1540421699355 3 40.26061045350342 4 40.36566300713766 5 40.20372298545202 6 40.72010338614982 7 40.20316623763488 8 40.215104318702416 9 40.14469491803472 10-14 40.23115772700409 15-19 40.269572672754904 20-24 40.57766536479521 25-29 40.81856455758115 30-34 40.94724699658631 35-39 41.11267017771503 40-44 41.19111718765214 45-49 41.28555628152022 50-54 41.26044972709326 55-59 41.3243944254208 60-64 41.33571608600269 65-69 41.381943821417416 70-74 41.40895707810531 75-79 41.4101986655375 80-84 41.40539747420055 85-89 41.42473998671366 90-94 41.47264557928672 95-99 41.47718136433514 100-101 41.59510195189992 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 321.0 1 293.0 2 322.5 3 506.0 4 743.5 5 1002.0 6 1476.5 7 2111.0 8 2937.5 9 3682.5 10 4331.5 11 5500.0 12 7406.5 13 9655.5 14 12046.0 15 15663.0 16 20999.5 17 29376.0 18 42790.0 19 62361.5 20 89244.0 21 124571.5 22 169036.5 23 221613.5 24 283471.0 25 354538.5 26 431816.5 27 513774.0 28 598566.5 29 684396.5 30 769346.5 31 850645.0 32 927624.5 33 1003555.5 34 1075696.5 35 1142289.0 36 1203263.0 37 1248976.5 38 1271276.5 39 1267681.5 40 1239564.0 41 1190221.0 42 1123814.0 43 1049276.0 44 978434.0 45 918750.0 46 871407.0 47 836484.0 48 807032.5 49 774584.5 50 735764.0 51 688762.5 52 637730.0 53 589531.0 54 545403.5 55 504936.0 56 468573.0 57 431145.5 58 388773.0 59 343155.0 60 295085.0 61 247621.5 62 205636.5 63 170354.0 64 141113.5 65 116791.0 66 96654.0 67 79012.5 68 63904.0 69 51625.5 70 41226.5 71 32866.0 72 26296.0 73 21018.0 74 17060.5 75 13957.0 76 11506.0 77 9599.0 78 8074.5 79 6649.5 80 5242.5 81 4015.0 82 3040.5 83 2266.5 84 1487.5 85 905.5 86 545.5 87 333.0 88 229.0 89 171.0 90 129.0 91 117.0 92 88.0 93 57.0 94 55.0 95 42.5 96 25.0 97 15.5 98 10.0 99 7.5 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1304005778146426 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 2.4359822402565854 15-19 9.023154277795851E-5 20-24 2.3613786726997654E-4 25-29 0.0 30-34 1.862225457332335E-4 35-39 1.324675840782795E-4 40-44 1.9582164602876103E-4 45-49 0.0013221160807039877 50-54 1.7726338545740784E-4 55-59 2.34218047210871E-4 60-64 0.0 65-69 0.003010277852677424 70-74 0.018720805336357435 75-79 0.015589578819956363 80-84 0.0012555623186549971 85-89 0.006974706274730282 90-94 0.00809076166909028 95-99 0.004627406282463957 100-101 0.004409186735745632 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3.1252929E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Duplicate Percentage 7.6469443654488085 #Duplication Level Relative count 1 100.0 2 4.0425485937787 3 0.5823690209933767 4 0.4656791243935907 5 0.3841042429742958 6 0.3462880727799207 7 0.27497758041338477 8 0.2544488023078668 9 0.2042073190496256 10++ 1.4915777986667098 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Kmer Content warn #Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position CCCAG 5708155 3.648385 4.0397215 25-29 CTGGG 5548925 3.5038776 3.699052 85-89 CCAGG 5110720 3.256511 3.5420907 25-29 GGAGG 4974090 3.150034 3.41407 70-74 CCTCC 4905040 3.1259778 3.4692996 70-74 CCTGG 4893155 3.0992987 3.2757812 85-89 >>END_MODULE