##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	ZL4_GCCAAT_L008_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	13406231	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.18936187210261	34.0	33.0	34.0	31.0	34.0
2	33.35986124660988	34.0	34.0	34.0	31.0	34.0
3	33.401884317822066	34.0	34.0	34.0	31.0	34.0
4	36.65040233903175	37.0	37.0	37.0	35.0	37.0
5	36.58974218779312	37.0	37.0	37.0	35.0	37.0
6	36.593670286600315	37.0	37.0	37.0	35.0	37.0
7	36.59163996204452	37.0	37.0	37.0	35.0	37.0
8	36.58460659077111	37.0	37.0	37.0	35.0	37.0
9	38.451546523403934	39.0	39.0	39.0	37.0	39.0
10-14	38.757750213314985	39.4	39.2	39.4	37.2	39.4
15-19	40.045122033179936	41.0	40.0	41.0	38.0	41.0
20-24	39.98459053853391	41.0	40.0	41.0	38.0	41.0
25-29	39.849812031435235	41.0	40.0	41.0	38.0	41.0
30-34	39.6860346953592	41.0	40.0	41.0	37.8	41.0
35-39	39.50768480716168	41.0	40.0	41.0	37.0	41.0
40-44	39.31068236851954	41.0	39.2	41.0	36.4	41.0
45-49	39.155597363643814	40.2	39.0	41.0	35.8	41.0
50-54	39.08539649958291	40.4	39.0	41.0	35.4	41.0
55-59	38.78919016090354	40.0	38.2	41.0	35.0	41.0
60-64	38.53974916589159	40.0	37.6	41.0	35.0	41.0
65-69	37.98034886911914	39.4	36.4	41.0	34.2	41.0
70-74	37.245642432985086	38.4	35.2	40.4	34.0	41.0
75-79	36.08961012233789	36.4	34.8	39.2	32.8	40.2
80-84	35.679815527570724	35.8	35.0	37.8	33.0	39.4
85-89	34.9406982618754	35.0	35.0	36.6	33.0	38.0
90-94	34.41506587496516	35.0	35.0	35.8	33.0	36.8
95-99	34.081859069860876	35.0	35.0	35.0	32.0	36.0
100-101	33.69987657231924	35.0	34.0	35.0	31.5	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	5.0
11	4.0
12	25.0
13	94.0
14	353.0
15	1087.0
16	2841.0
17	5273.0
18	7610.0
19	9535.0
20	11236.0
21	12756.0
22	15146.0
23	17643.0
24	21388.0
25	25892.0
26	31725.0
27	39262.0
28	48577.0
29	61043.0
30	76574.0
31	97370.0
32	128584.0
33	177088.0
34	274188.0
35	475577.0
36	1009530.0
37	2579423.0
38	5349918.0
39	2878267.0
40	48215.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.079754445425746	31.170885225017	29.947840417611282	19.801519911945974
2	20.067668534131627	30.357368898089256	30.54250668961321	19.03245587816591
3	20.179253217267416	29.461624960139638	30.377411901605043	19.981709920987903
4	20.215458262047996	29.331960636767302	30.423954082195902	20.028627018988804
5	20.49587240440658	29.66007373735392	30.26050349274155	19.583550365497953
6	20.477259846527705	29.777107573676	29.62883287267576	20.116799707120542
7	20.501556328546034	30.045357267079766	29.852215734608777	19.600870669765424
8	19.98870525205779	29.94708206952424	30.043902719563757	20.020309958854206
9	19.979478199353718	29.749815589482232	30.284499797146562	19.98620641401748
10-14	20.011490179454615	29.864077383121323	30.109054513531802	20.015377923892256
15-19	20.030954010332167	29.708702485606075	30.14470188266167	20.115641621400087
20-24	20.18120205736964	29.570579307997175	29.97345990238222	20.274758732250962
25-29	20.335583063361852	29.546937234587965	29.791994928209053	20.32548477384113
30-34	20.466317664643277	29.516587293097995	29.674342354067427	20.342752688191293
35-39	20.52593930269452	29.4575750613361	29.612489764263238	20.403995871706144
40-44	20.584275761718203	29.434873561950837	29.547055837733083	20.433794838597876
45-49	20.599740916545205	29.382459086674206	29.51985390740102	20.49794608937957
50-54	20.562182162553754	29.393319103127023	29.522517302411178	20.521981431908046
55-59	20.615362094999202	29.357508208131932	29.50929278273381	20.517836914135053
60-64	20.572151218439355	29.36673712193697	29.482758718433168	20.578352941190513
65-69	20.584523302270263	29.35850442185034	29.445641562339333	20.611330713540067
70-74	20.64731401798737	29.38442164005118	29.403957818089577	20.564306523871874
75-79	20.632090195628404	29.358625300817543	29.4311227630408	20.57816174051325
80-84	20.64399810699896	29.372920951601078	29.43118171236031	20.551899229039655
85-89	20.60168173583607	29.381994917905697	29.395429862888367	20.620893483369866
90-94	20.596341341307664	29.34612513272965	29.397233386605976	20.660300139356714
95-99	20.615799004014807	29.403404021633666	29.330940984362226	20.6498559899893
100-101	20.677579879618037	29.358505169654187	29.281404380139413	20.68251057058836
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	38.881274357371716
2	39.10012441229754
3	40.16096313825532
4	40.244085281036796
5	40.07942276990453
6	40.59405955364824
7	40.10242699831146
8	40.009015210912004
9	39.9656846133712
10-14	40.02686810334687
15-19	40.146595631732254
20-24	40.455960789620605
25-29	40.66106783720298
30-34	40.80907035283457
35-39	40.92993517440066
40-44	41.01807060031608
45-49	41.097687005924776
50-54	41.0841635944618
55-59	41.13319900913426
60-64	41.15050415962987
65-69	41.195854015810326
70-74	41.21162054185924
75-79	41.21025193614166
80-84	41.19589733603861
85-89	41.222575219205936
90-94	41.25664148066438
95-99	41.26565499400411
100-101	41.3600904502064
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	336.0
1	331.5
2	382.0
3	563.5
4	732.5
5	817.0
6	1022.5
7	1373.0
8	1836.0
9	2310.5
10	3065.5
11	4396.5
12	6126.5
13	8007.0
14	10001.0
15	12721.0
16	16020.5
17	20292.0
18	26527.0
19	35125.5
20	46636.5
21	61216.5
22	79253.5
23	100766.5
24	126382.5
25	155901.5
26	188001.5
27	221787.5
28	256952.0
29	292102.5
30	326994.0
31	360653.5
32	392927.0
33	424798.0
34	454128.0
35	481585.0
36	506354.0
37	525353.0
38	534449.0
39	533226.0
40	523018.5
41	503480.5
42	476450.5
43	446397.5
44	417427.5
45	392863.0
46	373563.5
47	358847.0
48	346444.0
49	332213.5
50	315481.5
51	295279.5
52	273306.5
53	252670.5
54	233820.5
55	217003.0
56	201350.5
57	185531.0
58	167618.0
59	147911.5
60	127628.5
61	107467.0
62	89620.0
63	74645.0
64	61998.5
65	50943.0
66	41762.0
67	34095.5
68	27536.0
69	22126.5
70	17485.0
71	13852.0
72	11075.5
73	8787.5
74	7024.5
75	5734.5
76	4742.5
77	3969.0
78	3285.5
79	2640.0
80	2144.0
81	1706.5
82	1310.5
83	938.0
84	609.0
85	378.5
86	223.5
87	143.5
88	106.5
89	72.0
90	56.5
91	50.0
92	36.5
93	23.5
94	23.5
95	13.0
96	3.0
97	4.0
98	2.5
99	2.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13491487652271544
2	0.0
3	4.177162097236725E-4
4	1.11888270461698E-4
5	0.0
6	2.461541950157356E-4
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	1.4769251700944136E-4
20-24	1.909559815879646E-4
25-29	1.4918436061559733E-6
30-34	3.729609015389934E-5
35-39	1.1039642685554202E-4
40-44	9.995352161245021E-5
45-49	3.6400983990205745E-4
50-54	5.2214526215459063E-5
55-59	0.0019200027211227378
60-64	0.002078138143375271
65-69	0.0017663428296886723
70-74	0.01776039813128686
75-79	0.016228274747764677
80-84	0.004764948478062178
85-89	0.006649146952637173
90-94	0.010078895403189757
95-99	0.007845605524774264
100-101	0.002998605648373506
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.3406231E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	6.853683611646226
#Duplication Level	Relative count
1	100.0
2	4.220401733809828
3	0.7517105030513124
4	0.48756704046806437
5	0.3809452371129602
6	0.25985715893078154
7	0.20520678736183368
8	0.17681002566424311
9	0.11090810700757069
10++	0.564184718255903
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCCAG	2363480	3.546133	3.9064043	25-29
CTGGG	2304120	3.4194584	3.604647	85-89
GGAGG	2132255	3.1702218	3.4064727	70-74
CCAGG	2117595	3.1675858	3.44003	25-29
CCTCC	2086155	3.124291	3.4724233	70-74
CCTGG	2034240	3.0281124	3.2226746	85-89
AAAAA	8726020	3.0064235	3.442242	10-14
>>END_MODULE