##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	ZL4_GCCAAT_L007_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	13606977	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.22694408905079	34.0	33.0	34.0	31.0	34.0
2	33.371838726559176	34.0	34.0	34.0	31.0	34.0
3	33.398736986179955	34.0	34.0	34.0	31.0	34.0
4	36.64914367092705	37.0	37.0	37.0	35.0	37.0
5	36.58996381047752	37.0	37.0	37.0	35.0	37.0
6	36.58297129479972	37.0	37.0	37.0	35.0	37.0
7	36.58714084693463	37.0	37.0	37.0	35.0	37.0
8	36.58287193400856	37.0	37.0	37.0	35.0	37.0
9	38.443806511909294	39.0	39.0	39.0	37.0	39.0
10-14	36.86068176642027	39.4	38.4	39.4	26.2	39.4
15-19	39.53527067768248	41.0	39.0	41.0	36.6	41.0
20-24	39.97936682041867	41.0	40.0	41.0	38.0	41.0
25-29	39.844901170921354	41.0	40.0	41.0	38.0	41.0
30-34	39.68711626395782	41.0	40.0	41.0	37.8	41.0
35-39	39.51579957840746	41.0	40.0	41.0	37.0	41.0
40-44	39.322358742871394	41.0	39.2	41.0	36.4	41.0
45-49	39.16436201810292	40.0	39.0	41.0	36.0	41.0
50-54	39.085910162117564	40.0	39.0	41.0	35.4	41.0
55-59	38.783258294623415	40.0	38.2	41.0	35.0	41.0
60-64	38.530232203670224	40.0	37.6	41.0	35.0	41.0
65-69	37.966730214947816	39.4	36.4	41.0	34.2	41.0
70-74	37.20851849753255	38.2	35.2	40.4	34.0	41.0
75-79	36.05001222534587	36.2	34.8	39.2	32.8	40.2
80-84	35.62913486221076	35.8	35.0	37.6	33.0	39.2
85-89	34.91115087502537	35.0	35.0	36.4	33.0	37.8
90-94	34.392817111398074	35.0	35.0	35.8	33.0	36.6
95-99	34.08589628688283	35.0	34.8	35.0	32.2	36.0
100-101	33.72338907459019	35.0	34.0	35.0	31.5	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	6.0
12	26.0
13	90.0
14	332.0
15	1226.0
16	2872.0
17	5451.0
18	7808.0
19	9608.0
20	11147.0
21	12751.0
22	14824.0
23	17818.0
24	21053.0
25	25707.0
26	31709.0
27	39725.0
28	49369.0
29	62556.0
30	80058.0
31	102035.0
32	135093.0
33	190437.0
34	301986.0
35	545851.0
36	1219714.0
37	3065720.0
38	5193442.0
39	2419700.0
40	38861.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.033694464239094	31.128886557682783	30.01196803539666	19.82545094268147
2	20.05462344795615	30.381825441462862	30.540758612291324	19.022792498289665
3	20.142923736844708	29.49023137174407	30.386786131849856	19.980058759561363
4	20.21764275782931	29.318385707567522	30.427419697997575	20.036551836605586
5	20.4625832762119	29.66342928337426	30.2840741187407	19.589913321673137
6	20.48468223323961	29.783044389653924	29.645203339433877	20.087070037672586
7	20.496308621672544	30.021495590093227	29.891305026825577	19.590890761408648
8	20.00563387444544	29.970852453120187	30.026926627420625	19.996587045013744
9	19.987547564752994	29.763598483336896	30.260402439131045	19.98845151277907
10-14	20.148008502400067	29.920499055410232	29.99843164670904	19.93306079548066
15-19	20.02726485951085	29.697914863515418	30.151554585418435	20.123265691555297
20-24	20.171262258869262	29.575773523051563	29.981969059592377	20.270995158486794
25-29	20.34246989614225	29.565364885969895	29.782649004257156	20.309516213630697
30-34	20.476156564956167	29.516455300462933	29.681413612584684	20.32597452199622
35-39	20.513111464943062	29.466215293794907	29.61697194765362	20.40370129360841
40-44	20.57523312119968	29.43086523008187	29.56335922410981	20.430542424608635
45-49	20.589444205446256	29.38510968958633	29.53120431511379	20.494241789853625
50-54	20.56361336469769	29.39021905861063	29.52913187317777	20.51703570351391
55-59	20.598137635894055	29.363193131317722	29.51585785426083	20.522811378527393
60-64	20.570927693932312	29.3687628045524	29.487529816505166	20.572779685010122
65-69	20.58635562520482	29.365149487501974	29.454457689579662	20.59403719771355
70-74	20.637357314629973	29.388247423656438	29.413128872556793	20.5612663891568
75-79	20.62045481624556	29.365736211810972	29.43494498278307	20.578863989160396
80-84	20.642487599845072	29.388959163362205	29.42567755321385	20.542875683578877
85-89	20.600747461607153	29.375022140885854	29.41288617930994	20.611344218197054
90-94	20.588649820302898	29.35336943199829	29.40766207260917	20.650318675089643
95-99	20.610183329517472	29.389838718989953	29.348738831255073	20.65123912023751
100-101	20.691918564613488	29.36236483353018	29.280748649345856	20.66496795251048
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	38.85914540692056
2	39.077415946245814
3	40.122982496406074
4	40.2541945944349
5	40.05249659788504
6	40.57175227091219
7	40.0871993830812
8	40.002220919459184
9	39.975999077532066
10-14	40.08106929788073
15-19	40.15053055106615
20-24	40.44225741735606
25-29	40.65198610977295
30-34	40.80213108695238
35-39	40.91681275855147
40-44	41.00577554580832
45-49	41.08368599529988
50-54	41.0806490682116
55-59	41.12094901442145
60-64	41.14370737894244
65-69	41.180392822918364
70-74	41.198623703786765
75-79	41.19931880540595
80-84	41.185363283423946
85-89	41.2120916798042
90-94	41.23896849539254
95-99	41.26142244975498
100-101	41.35688651712396
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	288.0
1	302.0
2	375.5
3	541.5
4	700.5
5	794.0
6	991.0
7	1325.5
8	1844.0
9	2401.5
10	3107.5
11	4329.0
12	6050.5
13	8000.5
14	10156.5
15	13032.0
16	16414.5
17	20863.0
18	27138.0
19	35658.5
20	47280.5
21	62279.5
22	81233.5
23	103461.0
24	129182.5
25	159098.5
26	192121.0
27	226706.5
28	262631.5
29	298810.0
30	333840.0
31	367642.5
32	400540.0
33	432843.0
34	462512.0
35	490305.0
36	516033.5
37	535757.5
38	544517.0
39	542930.5
40	532163.0
41	510249.5
42	481994.5
43	452094.0
44	422840.5
45	397231.5
46	377928.0
47	363988.5
48	350862.0
49	336355.5
50	319069.5
51	298837.5
52	277363.0
53	255916.5
54	236588.0
55	219558.0
56	203626.0
57	187444.5
58	168563.5
59	148388.0
60	128049.0
61	107490.0
62	89309.5
63	74366.0
64	61605.5
65	50653.5
66	41710.0
67	34047.0
68	27426.5
69	21938.5
70	17362.5
71	13714.0
72	10901.0
73	8688.5
74	7001.5
75	5693.5
76	4702.0
77	3937.0
78	3270.0
79	2650.5
80	2105.5
81	1628.5
82	1221.5
83	898.5
84	605.5
85	367.0
86	230.0
87	137.5
88	77.5
89	55.0
90	42.5
91	37.5
92	30.0
93	23.0
94	16.5
95	12.0
96	7.0
97	4.0
98	2.5
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12820628711285392
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	2.4298681477891817
15-19	8.525038294692494E-5
20-24	2.41052806953374E-4
25-29	0.0
30-34	1.7638010264881023E-4
35-39	1.1317723253298658E-4
40-44	1.5139292144022878E-4
45-49	0.0013184412672998566
50-54	1.8372927359251067E-4
55-59	2.5869081721825504E-4
60-64	0.0
65-69	0.0028514783261557658
70-74	0.018106887371089113
75-79	0.015095197118360677
80-84	0.001258178065561513
85-89	0.006812681464810295
90-94	0.007947393458517641
95-99	0.004510921125243322
100-101	0.004460946762826159
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.3606977E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	6.291103238361368
#Duplication Level	Relative count
1	100.0
2	3.7864346492582612
3	0.7337515042438313
4	0.495734869703198
5	0.34184939457513763
6	0.2641079434723805
7	0.19648353052683146
8	0.13791120435352125
9	0.09904047880214269
10++	0.43982492199231105
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCCAG	2381715	3.546722	3.939791	25-29
CTGGG	2326120	3.4249766	3.5986097	1
CCAGG	2129190	3.160945	3.3913953	25-29
GGAGG	2140255	3.1578994	3.4083345	70-74
CCTCC	2104045	3.1266918	3.4848785	70-74
CCTGG	2052835	3.0318968	3.2113476	70-74
>>END_MODULE