FastQCFastQC Report
Mon 21 Jul 2014
ZL3_ACAGTG_L008_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename ZL3_ACAGTG_L008_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 7195243
Filtered Sequences 0
Sequence length 101
%GC 41

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC 400945 5.5723621842931506 TruSeq Adapter, Index 5 (100% over 50bp)

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 9221355 5.562278 25.595173 65-69
CACAC 2113175 3.6893888 16.536957 30-34
CTGGG 1311070 3.4488678 3.7018197 1
CCCAG 1354650 3.369331 3.8836675 25-29
TTTTT 4147135 3.3222866 3.8904967 15-19
GGAAG 1840365 3.2096288 75.78967 5
CCTCC 1169910 3.080855 3.5426738 65-69
CCTGG 1159160 3.050353 3.2909825 65-69
GGAGG 1223000 3.0386095 3.3797126 4
CTCCA 1477950 2.7310202 17.521141 20-24
CAGTG 1474350 2.722411 17.481146 35-39
TCCAG 1450160 2.678706 17.203089 25-29
CTGCT 1337880 2.6156068 18.43888 55-59
CACAG 1470190 2.56588 16.826908 30-34
TCTGC 1283750 2.5097806 18.289454 55-59
AGAGC 1428885 2.4928958 74.88423 8
GAGCA 1376160 2.4009094 74.72939 9
TTCTG 1650410 2.3962917 13.908242 55-59
GCACA 1359360 2.3724518 15.973051 10-14
AGCAC 1353115 2.3615525 15.862683 10-14
GAAGA 1900155 2.3261743 53.175755 6
CGTCT 1178045 2.3031232 16.86976 50-54
CTTCT 1564840 2.2728658 14.08051 50-54
AAGAG 1854720 2.2705526 53.10785 7
GTCTG 1150295 2.2480633 17.741152 15-19
ACTCC 1201120 2.2194817 17.15015 20-24
ACACA 1802940 2.2087498 11.962922 30-34
TCTTC 1443430 2.0965233 13.968934 50-54
CCAGT 1129480 2.086352 16.822737 25-29
GATCG 1122380 2.0724926 78.83819 1
TGCTT 1396940 2.0282693 13.984995 55-59
TCTGA 1447055 1.9851197 13.051907 15-19
GCTTG 1010485 1.9748274 17.929356 55-59
ATGCC 1062995 1.9635426 16.837372 45-49
CTGAA 1498825 1.9427027 12.321903 15-19
ATCGG 1040405 1.9211245 78.74357 2
GAAAA 2227475 1.9141166 8.75548 60-64
TCGGA 1008845 1.8628483 78.665665 3
AGTGA 1424420 1.8455995 12.147492 35-39
CAGTC 998600 1.8445935 16.63265 25-29
TCACA 1399345 1.8144137 12.065857 30-34
CGGAA 1002745 1.7494332 74.340614 4
CTTGA 1275195 1.7493563 12.672821 60-64
ATCTC 1274720 1.749333 12.421955 40-44
GTCAC 932010 1.7215899 16.743193 25-29
GTCTT 1175375 1.7065707 13.436787 50-54
TGAAA 1875810 1.7060454 8.940398 60-64
GTGAT 1163795 1.5959601 12.532661 35-39
AACTC 1222730 1.5854117 11.910497 20-24
TGAAC 1218815 1.5797677 11.764915 20-24
AGATC 1212990 1.5722177 6.64527 95-97
ACAGT 1203705 1.5601829 12.023053 30-34
TTGAA 1594385 1.5347625 9.265241 60-64
GAACT 1175650 1.5238194 11.831293 20-24
CCGTC 574140 1.511404 22.242311 50-54
AGTCA 1144335 1.4832305 12.067745 25-29
TGCCG 557170 1.4662043 23.05722 45-49
CACGT 786720 1.453213 16.162943 10-14
TGATC 1045025 1.4336011 12.388545 35-39
GCCGT 534410 1.4063107 22.73435 45-49
GATCT 1024785 1.4058352 12.313768 35-39
ACACG 795180 1.3878046 15.298661 10-14
TCTCG 645105 1.2612052 16.682608 40-44
ACGTC 682410 1.2605338 15.743968 15-19
TATGC 893045 1.2251097 12.258097 45-49
GTATG 889175 1.2193625 12.076926 45-49
CTCGT 560720 1.0962292 16.57642 40-44
CGTAT 543080 0.7450157 11.864116 40-44
TCGTA 518535 0.7113441 11.950204 40-44
Produced by FastQC (version 0.10.1)